印度洋深海沉积物样品纯培养细菌的分离鉴定

为探索印度洋深海沉积物中纯培养细菌的多样性,本文对采自印度洋12个沉积物样品进行细菌纯培养分离,共获得343株细菌。所有细菌采用16S rRNA 基因进行比对分析,鉴定为4个门：厚壁菌门（Firmicutes）、变形菌门（Proteobacteria）、放线菌门（Actinobacteria）和拟杆菌门（Bacteroidetes）,5个纲,13个目,26个科,39个属下的121个种。分离出的优势类群为链霉菌属（Streptomyces,分离率为16.53%）和芽孢杆菌属（Bacillus,分离率为8.26%）,其中可能有12个潜在的新分类单元。研究结果表明,印度洋深海沉积环境中纯培养细菌资源丰富,潜在新物种较多;其次,分离培养方法与从海洋沉积物中获得的细菌物种多样性直接相关。     

印度洋中央盆地深海沉积物岩心的地球化学

印度洋中央盆地深海沉积物岩心的地球化学Ａ．Ｖ．Ｍｕｄｈｏｌｋａｒ等用４个采自印度洋中央盆地（ＣＩＯＢ）的沉积物岩心研究粗粒部分、过渡金属和主要氧化物在沉积物岩心中的分布型式及其在铁锰结核生长认识上的意义，试图追踪地下陆源输入对印度洋中央盆地的影响．在...     

南大西洋深海沉积物中可培养放线菌的多样性

探究了南大西洋深海沉积物中可培养放线菌的多样性,筛选药源活性次级代谢产物产生菌,为后续资源开发奠定基础.采用3种预处理方法及8种选择性培养基对南大西洋3个深海沉积物样品中的放线菌菌株进行选择性分离鉴定;利用兼并引物扩增法,选取代表菌株进行聚酮合酶（PKSⅠ、PKSⅡ）基因和非核糖体多肽合成酶（NRPS）基因的检测;以4株细菌为指示菌检测代表菌株的抑菌活性.共分离得到132株放线菌纯菌株,分布于放线菌亚纲的6个目、13个科、19个属中,其中有5个属为较新或较稀有种属,有2株为潜在新种.34株化合物合成基因检测菌中PKSⅠ基因、PKSⅡ基因呈阳性的比率均为17.64%,NRPS基因呈阳性的则为52.94%.抗枯草芽孢杆菌（Bacillus subtilis）和抗副溶血性弧菌（Vibrio parahaemolyticus）的菌株分别有47.06%和7.82%.南大西洋深海沉积物中放线菌资源丰富,存在较多潜在新分类单元,筛选到的活性菌株可用于后续药源活性次级代谢产物的分离.     

南海北部巴士海峡深海沉积物中细菌多样性分析

从南海北部巴士海峡深海沉积物中提取到高质量的总DNA, 通过TA克隆构建了含有23个可操作分类单元(OTU)的16S rRNA基因文库, 选择各OTU中代表性克隆子进行测序与系统发育分析, 结果表明, 南海北部巴士海峡深海沉积物中细菌在系统进化树中至少分属于9个类群: 其中放线菌Actinobacteria, 变形细菌Proteobacteria, 和浮霉菌Planctomycetes为优势种;其他细菌如疣微菌Verrucomicrobia, 鞘脂杆菌Sphingobacteria, 硝化螺旋菌门(Nitrospira), 绿弯菌Chloroflexi, 厚壁菌Firmicute, 酸杆菌Acidobacteria等为非优势种群;另外有两个克隆子属于未知种群.在所获得的23个代表克隆子序列中,有11个序列与已知细菌的同源性≤95%, 占到了所有序列的48%, 这一结果说明在南海北部巴士海峡海区具有非常丰富的微生物多样性, 这一海区蕴含着大量未知的微生物资源, 因而值得进行进一步的研究探索.     

深海超长沉积物柱状取样系统关键技术优化及应用

作为深海科学研究的重要基础装备,深海超长沉积物柱状取样系统在海底地形地貌、海洋地质构造、气候环境变化等研究领域发挥了重要作用,其在海底矿产资源勘探、海洋工程地质勘察等方面展现了巨大应用价值。针对传统超长柱状沉积物取样系统在作业过程中存在的盲目采样、安全隐患大、甲板作业空间受限等问题,自主研制了一套适用于深海的超长可控可视沉积物柱状取样系统,该系统具有液压锤击、立式收放和实时监控功能,能获取连续、超长、具有精准姿态方位信息的柱状沉积物样品。现通过对独立排缆方式、取样管快速拆装、样品封堵、低扰动取样刀头等关键技术细节进行优化,解决了样品扰动大、取样率低的问题,提高了取样作业效率与样品质量,且降低了作业安全风险。2017—2021年,该取样系统搭载不同母船在南海1 700,1 725 和1 778 m水深分别获取了15.25、16.01和15.83 m的长柱状沉积物样品。该设备的成熟应用,可为我国深海探测研究提供技术支持。     

印度洋深海热液区可培养细菌的分子鉴定与系统发育分析

采用MMJHS寡营养培养基从印度洋深海热液区沉积物和热液硫化物中分离获得16株细菌,通过16S rDNA序列比对和生理生化分析,对它们进行了鉴定并构建了系统发育树.结果表明,12株细菌属于γ-变型菌(γ-Gammaproteobacteria),其中6株属于盐单胞菌属(Halomonas),4株属于嗜冷杆菌属(Psychrobacter),2株属于食碱菌属(Alcanivorax);其余4株属于芽孢杆菌(Bacillus),其中1株为地衣芽孢杆菌(Bacillus licheniformis).分离获得的16株细菌中,4株为革兰氏阳性细菌,12株为革兰氏阴性细菌;H2S反应、吲哚测定反应、M.R和V-P均呈阴性.本研究为深入认识和开发利用深海热液区微生物资源奠定了基础.     

深海沉积物地热探针贯入沉积物深度的探讨

针对新型自容式高精度深海沉积物地热探针贯入沉积物深度、插入过程的运动轨迹以及探针的稳定性问题,进行了深入研究,对探针的结构设计以及探针下放要求具有重要指导意义.     

西南印度洋中脊表层沉积物中存在热液组分的地球化学证据

Hydrothermal materials in deep-sea sediments provide a robust tracer to the localized hydrothermal activity at mid-ocean ridges. Major, trace and rare earth element(REE) data for surface sediments collected from the ultraslow spreading Southwest Indian Ridge are presented to examine the existence of hydrothermal component.Biogenic carbonate oozes dominate all the sediment samples, with CaO content varying from 85.5% to 89.9% on a volatile-free basis. The leaching residue of bulk sediments by ~5% HCl is compositionally comparable to the Upper Continental Crust(UCC) in SiO_2, Al_2O_3, CaO, MgO, alkali elements(Rb, Cs) and high field strength elements(Nb, Ta, Zr, Hf, Ti). These detritus-hosted elements are inferred to be prominently derived from the Australian continent by means of eolian dust, while the contribution of local volcaniclastics is insignificant. In addition, the residual fraction shows a clear enrichment in Fe, Mn, and Ba compared with the UCC. Combining the positive Eu anomaly of residual fraction which is opposed to the UCC but the characteristic of hydrothermal fluids and associated precipitates occurred at mid-ocean ridges, the incorporation of localized hydrothermal component can be constrained. REE mixing calculations indicate that more than half REE within the residual fraction(~55%–60%) are derived from a hydrothermal component, which is inferred to be resulted from a diffuse fluid mineralization. The low-temperature diffuse flow may be widely distributed along the slow-ultraslow spreading ridges where crustal faults and fissures abound, and probably have a great mineralization potential.     

雅浦海沟深海沉积物可培养真菌多样性及其反硝化能力研究

自雅浦海沟4个站位的12个沉积物样品中分离得到38株真菌菌株,基于形态观察和ITS序列信息对所得菌株进行分类鉴定,发现它们分别属于青霉属(Penicillium,17株)、曲霉属(Aspergillus,7株)、篮状菌属(Talaromyces,1株)、枝孢属(Cladosporium,6株)、赭霉属(Ochroconis,1株)、Meyerozyma属(1株)、梅拉菌属(Meira,1株)、Cystobasidium属(2株)、Wallemia属(1株)和红酵母属(Rhodotorula,1株)等10个属.其中青霉属、曲霉属和枝孢属菌株为优势菌株,分别占分离菌株总数的44.7%、18.4%和15.8%;而Wallemia属(节担菌纲)菌株是首次从深海环境中分离到.利用PCR技术在分离的深海真菌菌株中检测nir K和P450nor两种反硝化关键酶基因,结果显示nir K基因可在黄曲霉(Aspergillus flavus)、黑曲霉(Aspergillus niger)、杂色曲霉(Aspergillus versicolor)、枝孢菌(Cladosporium sp.)、枝状枝孢(Cladosporium cladosporioides)和产黄青霉(Penicillium chrysogenum)等6种真菌菌株中检测到,而P450nor基因仅在黄曲霉菌株中检测到.同时还通过富集培养方法从上述沉积物样品中分离获得两株反硝化枝孢菌.结果表明,雅浦海沟深海沉积物环境中存在着丰富的真菌资源,它们在深海环境氮循环中起着一定作用,这将增强人们对深海环境可培养真菌多样性及其生态作用的认识.     

东太平洋深海沉积物中DNA的提取及细菌多样性初步分析

以东太平洋海隆附近深海柱状沉积物为材料,通过化学裂解和酶消化相结合的方法提取了沉积物微生物的总基因组DNA,并进行了纯化。结果表明所得到的DNA分子片段大小在21kb左右,纯化后的DNA可直接用于PCR等分子生物学操作。细菌16SrDNAV3可变区的PCR—DGGE图谱展示出15条以上条带,表明深海沉积物中细菌多样性较高,群落结构比较复杂。对其中9条主要条带进行回收、测序和系统发育分析,结果表明所获得的序列分属放线菌门（Actinobacteria）,绿弯菌门（Chloroflexi）,γ-变形细菌亚门（Gamma—proteobacteria）,α-变形细菌亚门（Alpha—proteobacteria）和嗜酸菌门（Acidobacteria）5个大类群。     

Microscale Response of Sediment Variables to Benthic Disturbance in the Central Indian Ocean Basin

Abstract

The response of sediment bacteria and biochemical variables to benthic disturbance was investigated in the Central Indian Ocean Basin from cores at nine stations. Generally, bacterial density and biochemical variables declined vertically with depth in the upper layers of the cores. Mechanical disturbance caused by a hydraulic disturber brought about a substantial decrease in total bacterial numbers. However, the numbers of retrievable bacteria increased by two orders of magnitude. The biochemical parameters decreased in quantity with a shift in the linear relationship. The data suggest that abiotic artifacts will directly affect the biomass and biochemistry of the sediment.     

Western Indian Ocean SST signal and anomalous Antarctic sea-ice concentration variation

Teleconnection between El Nino/La Nina-Southern Oscillation (ENSO) phenomenon and anomalous Antarctic sea-ice variation has been studied extensively.In this study,impacts of sea surface temperature in the Indian Ocean on Antarctic sea-ice change were investigated during Janaury 1979 and October 2009.Based on previous research results,sea areas in the western Indian Ocean (WIO;50°–70°E,10 °–20 °S) are selected for the resreach.All variables showed 1-10 year interannual timescales by Fast Founer Tranaform (FFT) transformation.Results show that i) strong WIO signals emerged in the anomalous changes of Antarctic sea-ice concentration;ii) significant positive correlations occurred around the Antarctic Peninsula,Ross Sea and its northwest peripheral sea region iii) negative correlation occurred in the Indian Ocean section of the Southern Ocean,Amundsen Seas,and the sea area over northern Ross Sea;and iv) the atmospheric anomalies associated with the WIO including wind,meridional heat flux,and surface air temperature over southern high latitudes were the possible factors for the teleconnection.     

印度洋海表温度的变化及其对印度夏季季风降水影响的诊断研究

对印度洋海表温度(SST)的主要特征及变化趋势进行分析,并研究了其与印度夏季季风降水(ISMR)和季风环流的关系,揭示出:从北印度洋到南半球中高纬度印度洋,SST最显著的变化模态是全海盆一致的变化,近50 a来总体趋势是上升的,在1976,1986年以及1996年间分别有一次跳跃性增温,与太平洋SST变化趋势基本一致.除了长期变化趋势外,南印度洋中高纬度比热带地区有更显著的模态分布.在印度洋SST升温的背景下,ISMR具有逐渐减少的趋势,但两者相关较弱.印度洋SST发生跳跃后的不同阶段,许多海区SST与ISMR相关均发生变化,但在春季,热带外南印度洋具有一对相对稳定区,其分布与EOF分析的第2模态相似.根据它们的分布,文中定义了春季南半球偶极子(SIOD),在正SIOD(PSIOD)情况下印度降水偏多,而负SIOD(NSIOD)则反之.环流分析表明,PSIOD(NSIOD)通过与大气的相互作用,对夏季马斯克林高压具有增强(减弱)作用,进而使得索马里越赤道气流增强(减弱),在印度地区低空产生异常的辐合(辐散),高层辐散(辐合),从而影响印度季风环流,使得印度季风降水偏多(少).     

Response of Meiofauna to Immediate Benthic Disturbance in the Central Indian Ocean Basin

Abstract

Twenty-four stations (12 each, during pre- and postdisturbance studies) 5300 to 5330 m deep between 10°01' and 10°03'S latitude and between 75°59' and 76°02'E longitude were sampled to study the effect of benthic disturbance on the distribution of meiofauna in the Central Indian Ocean. Bottom-sampling was conducted with a box corer. Total meiofauna density ranged from 35 to 45 organisms per 10 cm² of bottom area during the predisturbance period and 21 to 32 organisms per 10 cm² during the postdisturbance period in the test and reference sites. Differences between pre- and postdisturbance study results were statistically significant (P < 0.05). Nematoda was the most abundant meta-zoan group, on an average representing >55% of the meiofaunal population. The abundance of nematodes and harpacticoid copepods as well as total meiofauna showed marked decreases during postdisturbance sampling. Vertical distribution of meiofauna in the sediment cores revealed that 75% to 90% of the metazoan population was confined to the top 2-cm layer of the sediment. Recolonization experiments suggest that harpacticoid copepods may take more time for recolonization than the nematodes and are more sensitive to the physical disturbance. These findings suggest that nematodes and harpacticoid copepods can be used as indicator organisms in recolonization experiments.     

Study on low-temperature lipase of psychrophilic bacterium 2-5-10-1 isolated from deep sea of Southern Ocean

A strain of psychrophilic bacterium, 2-5-10-1, which produces low-temperature lipase, is isolated from the deep sea of Prydz Bay in Southern Ocean. The highest lipase secretion of this strain is observed at 5 ℃ and this temperature is also for optimal growth. Tween 80 and olive oil enhance secretion of lipase. The optimal temperature and pH for lipase activity are 35 ℃ and 7.5 ℃ respectively. At 0℃, the lipase still has 37% relative enzyme activity. The lipase shows high thermolability, more than 50% activity lost after incubation at 60 ℃ for 15 min. EDTA has no effect on lipase activity, indicating the lipase activity is independent of divalent cation. In contrast, the lipase activity is inhibited drastically by Cu2 and Zn2 .     

南大洋深海嗜冷菌2-5-10-1及其低温脂肪酶的研究

从南大洋普里兹湾的水样中筛选到一株产低温脂肪酶的深海嗜冷菌2-5-10-1,对其生长及产酶情况和酶性质做了初步研究.该菌株的最适生长温度为5℃,此时分泌的胞外脂肪酶最多;添加Tween80,橄榄油可显著促进脂肪酶的产生.该脂肪酶的最适作用温度为35℃,在0～20℃均保持较高的酶活性,在0℃可保持37%的相对酶活性;酶的最适作用的pH值为7.5,在pH6～9的范围内均存在较高酶活性;对热较敏感,在60℃保温15min可丧失50%以上的酶活性.该脂肪酶的催化作用不需要金属离子的参与,Cu2+和Zn2+对酶活有着强烈的抑制作用.     

中印度洋洋盆GC11岩心富稀土深海沉积的元素地球化学特征

对中印度洋洋盆的沉积GC11岩心开展了主量元素、微量元素和稀土元素分析研究,根据主微量元素相关性特征、稀土元素富集程度以及澳大利亚后太古代平均页岩归一化模式特征,初步探讨了GC11岩心的沉积地球化学特征,以及影响稀土元素富集的可能因素。研究表明,GC11岩心稀土元素总量在400.64×10?6～742.74×10?6,平均值为658.41×10?6,略低于邻近海域的GC02岩心,与沃顿海盆DSDP213岩心中含沸石型深海粘土层位中的稀土元素含量相当。δCe负异常明显,(La/Yb)_N为0.42,显示重稀土相对富集的特点。稀土元素与P₂O₅呈显著正相关性,CaO/P₂O₅的平均值为2.3,表明生物钙磷灰石可能是稀土元素的主要载体矿物,而铁锰水合物可能对富集稀土元素有一定的促进作用,但影响不大;GC11岩心中δCe负异常程度远低于GC02岩心,略低于DSDP213岩心,中稀土富集特征与GC02和DSDP213岩心基本一致。不同程度陆源物质的混入可能是导致以上不同岩心中稀土元素富集程度和分馏特征的主要原因。     

印度洋西北部公海鸢乌贼资源特征及其与海况的关系

根据2004年9月~2005年4月我国鱿钓船在印度洋西北部12°N~21°30′N,58°E~65°E获得的鸢乌贼资源调查资料,对其资源特征及其与海况关系进行了分析。研究认为,调查海域内鸢乌贼均有一定的分布,其中以14°30′N、60°E~63°E附近海域CPUE为最高。渔获物胴长范围为163~560 mm,优势胴长组为280~440 mm,占总数的70.51%,平均胴长为365.0 mm。渔获个体组成随纬度变化差异显著,而随经度变化差异不明显。16°N以南海域,雌性个体一般以Ⅰ、Ⅱ期为主;16°N以北海域,则以Ⅲ~Ⅴ期为主。分析还认为,鸢乌贼分布与表温、海面高度距平关系密切,9~11月适宜表温为27~28℃,12月~翌年3月为26~27℃,高产渔场大都分布在海面高度距平SSHA≤0的附近海域。     

赤道印度洋海温偶极子的气候影响及数值模拟研究

在分析研究印度洋海温变化的基本特征,尤其是在分析赤道印度洋海温偶极子及其影响的基础上,利用IAP9L大气环流模式模拟研究了赤道印度洋海温偶极子异常对亚洲季风区气候变化的影响.其结果表明,印度洋、亚洲南部和东部地区的流场和降水都对印度洋海温异常的强迫作用比较敏感.正位相印度洋偶极子的作用使得赤道东印度洋-印度次大陆南部-阿拉伯海一带出现距平东风,孟加拉湾-中南半岛出现异常反气旋性环流,从而对减少印度南部和中南半岛南部、印度尼西亚地区的夏季降水,以及增加中国南部和东非的夏季降水有十分重要的作用.与此相反,负位相印度洋偶极子的作用将使赤道东印度洋附近出现西风异常,孟加拉湾-中南半岛存在异常气旋性环流,从而使印度次大陆和中南半岛南部、印度尼西亚地区的降水增加,使中国西部和孟加拉湾的降水减少.数值模拟结果与资料分析相互映证,切实地揭示了印度洋海温偶极子对亚洲季风区的气候变化有重要影响.     

Distribution of Deep-Sea Benthos in the Proposed Mining Area of Central Indian Basin

Abstract

During the first stage of the project work on the Indian Deep-Sea Environment Experiment (INDEX), the abundance and distribution of deep-sea benthos were surveyed in the Central Indian Basin for the collection of baseline data. The deep-sea community of the sediment was characterized by a moderately high standing crop and diverse fauna. The macrofaunal component was dominated by polychaetes (100% prevalence) and peracarid crustaceans, whereas the meiofauna was dominated by nematodes and harpacticoid cope-pods. The results of this study conform to the general distribution reported elsewhere. The macrofaunal abundance showed an inverse relation to the abundance of polymetallic nodules. However, the relation between meiofaunal vertical distribution and the vertical profile of the total organic matter and total labile matter was positive.