5种波长光对日本沼虾人工育苗幼体成活率的影响

光照与日本沼虾繁殖和孵化关系极为密切。研究了不同波长的光对日本沼虾孵化的影响，结果表明：在环境光谱主峰值470．5nm、477．9nm、492．6nm、504．8nm和615．2nm条件下，适度成熟的亲虾都能顺利孵化，日本沼虾孵化与否，与孵化环境中的光波波长无关，而与抱卵日本沼虾胚胎发育有关。日本沼虾在孵化期对不同波长的光谱有明显的视觉敏感度分布，在前4种光谱条件下，日本沼虾视觉器官敏感，而在光谱主峰值615．2nm时日本沼虾视觉器官不敏感。这一特点在日本沼虾育苗生产上具指导意义，可防止抱卵亲虾在孵化过程中吞食幼体，提高幼体成活率。     

凡纳对虾人工繁殖的初步研究

研究了海水比重的变化对凡纳对虾亲虾人工繁殖活动的影响及换水对亲虾交配率的影响。试验采用剪眼柄、强化培育、控光、换水等技术措施 ,将凡纳对虾培育成熟并使之自然交配产卵 ,孵化出无节幼体 ;亲虾的平均产卵量可达 1 6万粒左右 ,卵的受精孵化率可达 80 %以上。结果表明 :适时换水可将亲虾的交配率从 4 6 %提高到 79% ;凡纳对虾人工繁殖的适宜海水相对密度为 1 0 1 6以上。     

褶皱臂尾轮虫在斑节对虾育苗中的应用

我们于 1 998年和 1 999年连续用轮虫代替部分配合饲料进行斑节对虾育苗 ,均取得很好的效果 ,现将我们其中一批育苗的做法、结果及体会报告如下 :1　材料和方法1 .1　育苗时间和地点于 1 999年 2月 2 3日至 4月 1 6日 ,在湛江市南三镇地聚中村虾苗场进行。1 .2　生产用幼体取自从新加坡进口的斑节对虾亲虾经培育产卵孵化所得。1 .3　生产用轮虫褶皱臂尾轮虫。1 .4　生产用水取自然海水 ,不经药物处理 ,经沙滤后使用。海水相对密度 1 .0 1 9～ 1 .0 2 0 ,p H值 8.2。1 .5　生产条件用于轮虫育苗的对虾育苗池 1 2个、每个 40 m3,共 480 m3水…     

氮浓度、温度和照度对波吉卵囊藻繁殖模式的影响

【目的】探索波吉卵囊藻(Oocystisborgei)的繁殖模式,调控其生殖过程以实现藻生物质稳定增长。【方法】在荧光显微镜下观察波吉卵囊藻似亲孢子形成过程,探究温度、照度、氮浓度对其繁殖模式的影响。【结果】波吉卵囊藻以2、4和8似亲孢子型模式繁殖,在藻细胞第二轮分裂过程中因分裂不同步,有时也形成3个似亲孢子。氮限制和弱光显著影响波吉卵囊藻繁殖模式(P 0.05)。通常情况下,波吉卵囊藻以4似亲孢子型繁殖模式繁殖,但在缺氮和弱光下2和3似亲孢子型模式的频率上升。在15～35℃温度范围内,波吉卵囊藻繁殖模式变化不大。【结论】波吉卵囊藻有4种繁殖模式。在不良环境中,2和3似亲孢子型模式频率上升。     

两种消毒剂对罗氏沼虾幼体的急性毒性试验

以半静水式试验法试验了碘伏制剂和TCCA（三氯异氰脲酸）两种消毒剂对罗氏沼虾各期幼体及仔虾的急性毒性。结果表明,不同发育期的蚤状幼体对消毒剂的耐受力不同。两种消毒剂对蚤状幼体的24hLC50、48hLC50和安全浓度分别为：碘伏制剂304×10-6～472×10-6、209×10-6～325×10-6、29．8×10-6～48．3×10-6,TCCA0．80×10-6～1．03×10-6、0．58×10-6～0．74×10-6、0．087×10-6～0．116×10-6;两种消毒剂对仔虾的24hLC50、48hLC50和安全浓度分别为：碘伏制剂499×10-6、355×10-6、53.6×10-6、TCCA1.19×10-6、0.84×10-6、0．124×10-6。在蚤状幼体阶段,ZⅪ对消毒剂的敏感性最大。     

三江源区毒杂草型退化草地植被光谱特征分析

毒草型退化草地具有群落演替特点,通过高光谱遥感技术反演毒杂草分布与退化草地群落结构能对该类退化草地进行有效监测,而光谱特征分析是毒杂草与优良牧草遥感识别的基础。本文选取了三江源区毒草型退化草地的8种典型毒杂草和4种优良牧草的地面实测高光谱数据作为研究样本,经过SG平滑、包络线去除、导数变换和光谱参量化对毒杂草种和优良牧草种的光谱特征进行了分析,并通过马氏距离法提取其特征识别波段。结果表明：① 8种典型毒杂草和4种优良牧草的 “近红外峰值”差异较大,其中鹅绒萎陵菜的“近红外峰值”达到60.07%,而最小者早熟禾仅为17.53%;② 经包络线去除处理后,植被光谱曲线中吸收谷和反射峰光谱差异更加明显,且可减少环境背景对植被光谱的影响,如沼泽草甸的鹅绒委陵菜和驴蹄草,其“绿峰幅值”分别为6.46%和6.89%,经处理后其“绿峰指数”分别为0.2866和0.3671,而在2种环境下生长的同一草种（狼毒草1和狼毒草2）的峰谷特征差异不明显;③ 基于马氏距离法提取的毒杂草与优良牧草的敏感识别波段主要分布在680~750 nm和900~1000 nm波长范围内,以醉马草与矮嵩草为例,其基于反射率的敏感识别波段为713.1~737.1 nm和934.6~965.6 nm。该研究可为利用高光谱遥感进行大面积毒杂草草种识别和植被群落生长监测提供重要科学依据,对于三江源区毒杂草的监测防治和畜牧业的可持续发展具有重要意义。     

淡水螯虾类胚胎离体孵化技术研究进展

【目的】综述淡水螯虾类胚胎离体孵化技术的主要研究成果,为提高孵化效果及出苗同步性,节省人力、物力,实现数字渔业与智慧渔业奠定基础。【方法】从胚胎与初孵幼虾生物学、影响离体孵化的环境因素、影响离体孵化的人为调控因素、离体孵化装置、研究展望等五个方面介绍国内外的一系列研究成果。【结果】离体孵化期间应将水温、水质控制在适宜水平,定期进行消毒,离体胚胎的发育阶段及孵化密度、孵化装置等是重要影响因素之一,必要的人工管理措施也是决定孵化成功与否的关键。【结论】离体孵化技术应用于淡水螯虾类意义重大,而控制适宜的环境条件及应用合理的管理措施是基础。今后在离体孵化装置的优化、离体孵化期间水质与菌群环境的维持、死卵与死虾的挑除、苗种生产的批量化与智能化等方面进行深入研究,实现孵化效率质的提升,更好地应用于实际生产。     

合浦绒螯蟹的繁殖生物学

为保护和开发合浦绒螯蟹资源 ,通过生态调查和实验 ,研究了该蟹的繁殖生物学 ,结果表明 :1、该蟹在淡水中生长 ,在盐度为 8～ 2 5的海水中繁殖 ;2、其生活史包括卵、氵蚤状幼体、大眼幼体、幼蟹、成蟹几个阶段 ;3、繁殖季节为 10月到翌年 3月 ,卵和幼体生长发育的适宜水温为 15～ 2 5℃。     

石油开发污染物毒性监测的实验生物筛选Ⅰ:钻井泥浆对水生动物的毒性比较

用静水式试验方法评价和比较了两种钻井泥浆的水可溶部份对斑节对虾仔虾、罗氏沼虾仔虾、虾虎鱼和剑尾鱼等4种水生动物的急性毒性。结果表明：泥浆水溶性成分对班节对虾仔虾、罗氏沼虾仔虾、虾虎鱼和剑尾鱼的半数致死浓度均明显大于10000mg／L（1％）,但斑节对虾仔虾对泥浆水可溶性成份的敏感性高于其余3种动物,适于钻井泥浆毒性试验。可以认为在试验条件下两种泥浆是无急性毒性的物质,但由于泥浆组成的复杂性,综合评价泥浆对水环境的影响时,需探讨其对底栖生物的影响。     

罗氏沼虾溞状幼体的形态特征

罗氏沼虾的幼体变态发育需经过多次蜕皮,每次蜕皮均伴随形态及附肢特征的改变。采用显微观测摄像法对Z1-Z12各期幼体样本30尾进行观察测量,结果显示:Z2与Z1在复眼上存在较大的差异;Z3的主要特征为尾节与第六腹节分界;Z4尾扇分为内肢和外肢,均具羽状刚毛;Z5无腹足,尾节狭长,且末端平直;Z6的腹部出现腹足萌芽;Z7腹肢芽延长分为内外肢,Z7与Z8的分期特征主要位于第1触角的内鞭、外鞭的分枝上;Z8的第1、第2对步足具不完全的螯;Z9第1、2对步足具完全的螯;Z10后额角上缘的齿刻增多,Z11第1触角内鞭和外鞭分节数量比Z10多;Z12的形态与成虾相似,额角上、下缘均有齿刻。     

Studies on the ingestion characteristics of giant freshwater prawn, Chinese prawn and giant tiger prawn

ＩＮＴＲＯＤＵＣＴＩＯＮＡｔｐｒｅｓｅｎｔｇｉａｎｔｆｒｅｓｈｗａｔｅｒｐｒａｗｎ (Ｍａｃｒｏｂｒａｃｈｉｕｍｒｏｓｅｎｂｅｒｇｉｉ) ,Ｃｈｉｎｅｓｅｓｈｒｉｍｐ (Ｐｅｎａｅｕｓｃｈｉｎｅｎｓｉｓ)ａｎｄｇｉａｎｔｔｉｇｅｒｐｒａｗｎ (Ｐ .ｍｏｎｏｄｏｎ)ａｒｅｔｈｅｍａｉｎｓｈｒｉｍｐｓｆｏｒｃｕｌｔｕｒｅｉｎＣｈｉｎａ.Ａｓｗｅｋｎｏｗ ,ｆｅｅｄｓｔｕｆｆｃｏｓｔｉｓａｍａｊｏｒｅｘｐｅｎｓｅｉｔｅｍｉｎｃｕｌｔｕｒｅｐｒａｃｔｉｃｅ.Ｏｖｅｒｆｅｅｄｉｎｇｗｉｌｌｎｏｔｏｎｌｙｗａｓｔｅｆｅｅ…     

Solar radiation penetrating through sea ice under very low solar altitude

Based on the optical data for transmitted radiation through sea ice in the Arctic during the late autumn and early winter of 2007,the authors studied the arriving solar radiation,reflected radiation and transmitted radiation under very low solar altitude.Through the atmosphere,the light of the arriving solar radiation at short wavelength was weakened,with the spectral distribution of double peaks centered at 490 nm and 683 nm.The magnitude of the peak at 683 nm even exceeded that at 490 nm under the very low solar radiation condition.The reflection was lower than that in summertime because of the thin thicknesses of ice and snow,allowing higher ratio of heat to enter the sea ice and snow.When higher ratio of solar radiation entered sea ice in late autumn,the new ice freezing would be affected.The spectral reflectivity from snow surface was almost a constant,but the reflection without snow decreased at longer wavelengths.In the transmission spectrum,the light of 490nm was dominant.It indicates that the radiation at longer wavelength was weakened by sea ice.Therefore,under the condition of low solar altitude,the radiation at shorter wavelength was weakened by the atmosphere while the radiation at longer wavelength was weakened by the sea ice.The combined effect of atmosphere and sea ice made the solar radiation under sea ice much weaker.The absorption of sea ice for the longer-wavelength radiation allowed the sea ice to gain more heat to slow down the freezing process.     

FOOD SOURCES AND CARBON BUDGET OF CHINESE PRAWN PENAEUS CHINENSIS

This study deals with contribution of artificial food pellet and natural food to Chinese prawn (Penaeus orientalis) growth in a semiintensive culture pond. The prawn carbon consumption, budget, and the effects of some factors on the budget were investigated. The results showed that 26.2% of P. orientalis growth carbon came from formulated feed at the initial culture stage (when the prawns were 0.06±0.01 g in wet weight), and was 62.5% when the prawns were 9.56±1.04 g. The remaining part of the growth carbon was derived from organic fertilizer and natural food. The highest growth rate occurred at 20×10-3 salinity. Suitable salinity for culturing Chinese prawn was (20-28)×10-3.     

Dietary effects of A zolla pinnata combined with exogenous digestive enzyme(Digestin~(TM)) on growth and nutrients utilization of freshwater prawn,Macrobrachium rosenbergii(de Man 1879)

The present study was conducted to evaluate the effect of either individual or combined wheat bran(WB) replacement with Azolla pinnata supplemented with Digestin~(TM) in the diet of freshwater prawn, Macrobrachium rosenbergii Postlarvae(PL) on growth performance, nutrient utilization, chemical body composition and survival(%). Experimental diets were a wheat bran-soybean based diet with no Azolla and Digestin~(TM)(control, T_1), and diets containing 17% Azolla supplemented with Digestin~(TM) 0%(T_2), 1%(T_3), 2%(T_4) and 3%(T 5). Each experimental diet was allocated into three tanks(6 m~3/tank) fed for 12 wks. Each tank was subdivided into three equal pens by nets(2 m~3) and stoked with 84 PL/m~2. The experimental diets were readily consumed by prawns PLs where both high growth and good feed efficiency were achieved for all diets. The results showed that the diets containing A. pinnata supplemented with Digestin? at the level up to 3% have the higher growth and better nutrient utilization than the control diet. No differences were observed for moisture and protein content among the experimental diets. However, the highest protein content was observed on prawns fed on diets T_1 and T 5 respectively, while the lowest value was recorded for T_4 diet. The results also show that prawn PLs fed the diets contain A. pinnata and supplemented with Digestin~(TM) recorded the highest values of body lipid content compared to the control diet. Feed efficiency and economic conversion rate(ECR) values show that economic performance and the cost-effectiveness of the A. pinnata supplemented with up to 3% Digestin~(TM) recorded the highest net return, and therefore it is recommended for prawn, M. rosenbergii PL's. These results are clearly indicating that A. pinnata have a good potential for use in prawn diets at reasonable levels than other conventional diets.     

星载高光谱Hyperion数据在海滩涂调查应用中的分析

以江苏苏北地区为研究区,较系统地分析了目前世界上星载传感器中光谱分辨率最高的EO-1卫星Hyperion数据的基本特点和处理方法,研究了将图像的灰度值转变为辐射能量值和反射率的方法和步骤,并选取了15类典型地物,进行了样本采集、辐射亮度和反射率反演,最后对典型地物的光谱曲线进行综合分析研究,并概括了苏北沿海滩涂典型地物的基本光谱特征。     

ONTOGENIC STUDY OF ISOZYMES IN GIANT TIGER PRAWN,PENAEUS MONODON (CRUSTACEA:DECAPODA)

The patterns of isozyme variation throughout the life cycle of the giant tiger prawn, Penaeusmonodon were described for ten enzyme systems (G3PD, MDH, LGG, GPI, MPI, FBP, PGM, LDH,PGD, and PROT). The zymograms showh little variation in the number of isozymic loci expressed duringlarval development compared with results reported for other penaeid prawns. There were specific ontogenicpatterns at some loci. As larvae developed beyond the embryonic stage, there was tendency for intensityof staining and complexity of banding patterns to increase.     

Ontogenic study of isozymes in giant tiger prawn,Penaeus monodon (Crustacea: Decapoda)

The patterns of isozyme variation throughout the life cycle of the giant tiger prawn,Penaeus monodon were described for ten enzyme systems (G3PD, MDH, LGG, GPI, MPI, FBP, PGM, LDH, PGD, and PROT). The zymograms showed little variation in the number of isozymic loci expressed during larval development compared with results reported for other penaeid prawns. There were specific ontogenic patterns at some loci. As larvae developed beyond the embryonic stage, there was a tendency for intensity of staining and complexity of banding patterns to increase.     

Photobehaviors ofthe calanoid copepod Calanus sinicus from the Yellow Sea to visible and UV-B radiation as a function of wavelength and intensity

The effects of wavelength-specific visible light,white light,and ultraviolet-B(UV-B,280-31 5 nm)on selected behaviors,grazing rate,spawning rate,and hatching rate of the marine copepod Calanus sinicus collected from the Yellow Sea were studied.Calanus s inicus placed in a partitioned experimental sy stem responded positively phototaxis to blue-cyan and yellow light but negatively to orange light and UVB.No obvious dodge activity was found among C.s inicus irradiated with0.005 mW/cm~2 UV-B.Under0.20,0.30 and 0.50 mW/cm~2 UV-B radiation,the lethal half times of individuals were 30.47,2.86,and1.96 h,respectively.Grazing of C.sinicus was restrained at0.10 mW/cm~2 UV-B,whereas yellow-red light stimulated grazing.Egg production rate was highest at a white-light intensity of 1.58 mW/cm~2,with an average rate of 10.04 eggs/(female d).These results are consistent with the observed phenomenon that C.sinicus in the Yellow Sea mostly spa.wn near dawn.Our results indicate that light intensity and spectrum are important factors affecting the diel vertical migration of C.sinicus under natural conditions in the Yellow Sea.     

Researches on the stability of porphyra-334 solution and its influence factors

1　Introduction　Ultravioletradiationisahighlyactivecomponentofsolarspectrum .Ultravioletradiationhasdetrimentaleffectsonlivingthingsexposedtosunlight ,includingthedestructionofDNA ,proteinsandothermolecules(Harm ,1980 ) ,inhibitionofphotosynthesisandgrow…     

对虾病毒病暴发前期病毒和弧菌相互作用关系

模拟天然继发感染研究对虾病毒病暴发前期病毒和弧菌的关系。结果显示 ,弧菌先感染再病毒感染组比病毒先感染再弧菌感染组死亡率高、死亡快、生长慢、免疫功能低下。表明弧菌的潜伏感染对病毒的增殖有利 ,而病毒的潜伏感染对弧菌的继发感染没有明显的促进作用。说明病毒性流行病的暴发前期可能是由弧菌先感染 ,使对虾体内环境发生变化而助长病毒感染 ,其中对虾的免疫力下降是关键