小波辅助的拟准检定法和补偿最小二乘估计解算污染半参数模型

利用拟准检定法探测粗差时,解算的污染半参数模型的标准化残差受到模型系统结构的干扰,难以准确初选拟准观测值。通过小波变换滤波分析的模极大值点初选拟准观测值,在拟准观测真误差估值范数极小的条件下实现对粗差的定位定值,最后采用补偿最小二乘估计分离系统误差,得到较为准确的参数估值。     

Evaluation of Ordinary Least Square (OLS) and Geographically Weighted Regression (GWR) for Water Quality Monitoring: A Case Study for the Estimation of Salinity

Landsat-5 Thematic Mapper (TM) dataset have been used to estimate salinity in the coastal area of Hong Kong. Four adjacent Landsat TM images were used in this study, which was atmospherically corrected using the Second Simulation of the Satellite Signal in the Solar Spectrum (6S) radiative transfer code. The atmospherically corrected images were further used to develop models for salinity using Ordinary Least Square (OLS) regression and Geographically Weighted Regression (GWR) based on in situ data of October 2009. Results show that the coefficient of determination (R²) of 0.42 between the OLS estimated and in situ measured salinity is much lower than that of the GWR model, which is two times higher (R² = 0.86). It indicates that the GWR model has more ability than the OLS regression model to predict salinity and show its spatial heterogeneity better. It was observed that the salinity was high in Deep Bay (north-western part of Hong Kong) which might be due to the industrial waste disposal, whereas the salinity was estimated to be constant (32 practical salinity units) towards the open sea.     

Application of Wet Waste from Shrimp (Litopenaeus vannamei) with or Without Sea Mud to Feeding Sea Cucumber (Stichopus monotuberculatus)

In the present study, the applicability of the wet waste collected from shrimp(Litopenaeus vannamei) to the culture of sea cucumber(Stichopus monotuberculatus) was determined. The effects of dietary wet shrimp waste on the survival, specific growth rate(SGR), fecal production rate(FPR), ammonia- and nitrite-nitrogen productions of sea cucumber were studied. The total organic matter(TOM) level in the feces of sea cucumber was compared with that in corresponding feeds. Diet C(50% wet shrimp waste and 50% sea mud mash) made sea cucumber grow faster than other diets. Sea cucumber fed with either diet D(25% wet shrimp waste and 75% sea mud mash) or sole sea mud exhibited negative growth. The average lowest total FPR of sea cucumber occurred in diet A(wet shrimp waste), and there was no significant difference in total FPR between diet C and diet E(sea mud mash)(P > 0.05). The average ammonia-nitrogen production of sea cucumber in different diet treatments decreased gradually with the decrease of crude protein content in different diets. The average highest nitrite-nitrogen production occurred in diet E treatment, and there was no significant difference in nitrite-nitrogen production among diet A, diet B(75% wet shrimp waste and 25% sea mud mash) and diet C treatments(P > 0.05). In each diet treatment, the total organic matter(TOM) level in feces decreased to different extent compared with that in corresponding feeds.     

非等间距GM(1,1)模型的总体最小二乘算法及其病态问题

在非等间距GM(1,1)模型中,系数矩阵中有无误差的常数项和有误差的随机项,并且系数矩阵与观测向量误差同源,即系数矩阵与观测向量中有相同的元素存在,这些相同元素应该有相同的改正数,为此本文推导了一种适合非等间距GM(1,1)模型求解的总体最小二乘算法。同时,考虑到非等间距GM(1,1)模型中存在病态问题时影响总体最小二乘计算结果的稳定性,提出对系数矩阵常数列乘以某一常数的方法,以改善病态问题。     

Analysis of drift bottle and drift card experiments in Bohai Sea and Huanghai Sea (1975–80)

The results of drift bottle and drift card experiments in the Bohai Sea and Huanghai Sea obtained by researchers of the Institute of Oceanology, Academia Sinica from 1975–1980 are reported in this article. Of over 50,000 bottles and cards released, around 10,000 were recovered. The results gave some convincing evidences for the existence of the Huanghai Sea Coastal Current, the Huanghai Sea Warm Current, the cyclonic movement around the northern Huanghai Sea Cold Water Mass, the anticyclonic eddy in the area near the Shidao-Qingdao coast and the flow of some of the Huanghai Sea water to the Japan Sea and to the North Pacific off Tokyo. The results show that the drift bottles and cards are still useful for getting the flow pattern of enclosed and semi-enclosed seas. Contribution No. 1312, Institute of Oceanology, Academia Sinica, Qingdao.     

基于最小二乘配置法的优化GM（1,1）预测模型及其应用

针对灰色GM(1,1)预测模型预测结果精度低、模型缺乏稳定性的问题,基于最小二乘配置理论的GM（1,1)预测优化模型,首先通过使得生成序列新预测值的误差在最小二乘意义下最小,选取GM(1,1)模型的最优初值,利用指数函数法构造新的背景值;然后将优化的GM(1,1)模型和最小二乘配置理论有机结合,进一步对优化的GM(1,1)模型进行改进,构建优化的灰色最小二乘配置预测模型;最后通过对建筑物的沉降数据进行定量分析与预报,与其他模型进行对比分析。     

Climatic variations in the coastal zone — Comparison between the Po River delta (Adriatic Sea,Italy) and the Huanghe River delta (Bohai Sea,China)

The aim of this work is to research the influence of natural climatic changes on the evolution of the coastal zone in modern times and the possible implication of human activities on the configuration of the present coastline. Comparison of data of two very far and different areas, the Po River delta, Adriatic Sea and the Huanghe River delta, Bohai Sea, reveals the planetary diffusion of climatic fluctuations and their effects on coastal evolution. Contribution No. 820 from the Marine Geology Institute, C. N. R., Italy. Contribution No.2207 from the Institute of Oceanology, Academia Sinica. Project supported by the National Natural Science Foundation of China.     

Mechanical Stress Induces Neuroendocrine and Immune Responses of Sea Cucumber(Apostichopus japonicus)

Grading procedure in routine sea cucumber hatchery production is thought to affect juvenile sea cucumber immunological response. The present study investigated the impact of a 3-min mechanical perturbation mimicking the grading procedure on neuroendocrine and immune parameters of the sea cucumber Apostichopus japonicus. During the application of stress, concentrations of noradrenaline and dopamine in coelomic fluid increased significantly, indicating that the mechanical perturbation resulted in a transient state of stress in sea cucumbers. Coelomocytes concentration in coelomic fluid increased transiently after the beginning of stressing, and reached the maximum in 1 h. Whereas, coelomocytes phagocytosis at 3 min, superoxide anion production from 3 min to 0.5 h, acid phosphatase activity at 0.5 h, and phenoloxidase activity from 3 min to 0.5 h were all significantly down-regulated. All of the immune parameters recovered to baseline levels after the experiment was conducted for 8 h, and an immunostimulation occurred after the stress considering the phagocytosis and acid phosphatase activity. The results suggested that, as in other marine invertebrates, neuroendocrine/immune connections exist in sea cucumber A. japonicus. Mechanical stress can elicit a profound influence on sea cucumber neuroendocrine system. Neuroendocrine messengers act in turn to modulate the immunity functions. Therefore, these effects should be considered for developing better husbandry procedures.     

Petroleum Exploration in the Yemeni Red Sea and Offshore Area (Tihama Basin)

The Red Sea is a pericratonic upper Tertiary basin which developed on the continental margin of Arabia. It is characterized by halokinetic tectonics in the upper structural stage and block- Rifting in the lower structural stage. This margin is overprinted on a Pre - rift Mesozoic platform. Hydrocarbon exploration interest in the Yemeni Red Sea sector and Offshore area (Tihama Basin) is based on three objectives, which cross pond to stages in geotectonic evolution; Mesozoic Pre - rift targets; Miocene Syn- Rift Salt targets; and upper Miocene- Pliocene Post - rift drift sequences. Reservoirs with appropriate sealing units ( salt or shale ) have been recorded. Models of source rock maturation using observed geothermal gradients are consistent with analytical results, and prove of oil and gas generation. Most traps are described as faulted, fault - blocks, listric - faults or structural. The evaporite group mainly consists of Halite and Anhydrite, that exhibits a strong halokinesis affecting a structural style- of Infra and Intra evaporite sequences in offshore (Tihama basin ).     

New genus and species of polyplacophora (Mollusca) from the East China Sea

The present paper dealing with two new species and one new genus of the Polyplacophora, is the first of a series of reports of systematic study on the deep sea mollusca collected by the research vesselsJINXING andKEXUE I of the Academia Sinica, Institute of Oceanology, during 1978 and 1981 from the continental margin to the bathyal zone of the East China Sea. Contribution No. 1647 from the Institute of Oceanology, Academia Sinica.     

Petroleum Exploration in the Yemeni Red Sea and Offshore Area (Tihama Basin)

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Effect of Co-Culture of Chinese Shrimp(Fenneropenaeus chinensis) and Sea Cucumber(Apostichopus japonicus Selenka) on Pond Environment

Monoculture of sea cucumber(pond S) and polyculture of shrimp with sea cucumber(pond SS) were established to evaluate the effect of shrimp on the environmental conditions of sea cucumber farming pond. Contributions of sediment organic matter(SOM2) resuspended from benthic sediment and the suspended particulate organic matter(SPOM) deposited from the water column to the precipitated organic matter(SOM1) collected with sediment traps were estimated with carbon stable isotope analysis. The results showed that the levels of SPOM and SOM2 in pond SS significantly decreased in comparison with those in pond S at the end of experiment(P 0.05), indicating that co-culturing shrimp in sea cucumber farming pond could purify the farming water. Carbon stable isotope analysis showed that the proportion of SOM2 in SOM1 in pond SS(84.97% ± 0.38%) was significantly lower than that in pond S(95.20% ± 0.30%)(P 0.05), suggesting that the resuspension of organic matter from benthic sediment into overlying water was reduced in polyculture pond. In contrast, the proportion of SPOM in SOM1 in pond SS(15.03% ± 0.38%) was significantly higher than that in pond S(4.80% ± 0.30%)(P 0.05), indicating that the sedimentation of SPOM from water column was enhanced in pond SS owing to the biodeposition effect of shrimp.     

Population genetic structure of the mantis shrimp Oratosquilla oratoria(Crustacea: Squillidae) in the Yellow Sea and East China Sea

The mantis shrimp O ratosquilla oratoria is an ecologically and economically important species in the Western Pacific. In present study, the population genetic structure of O ratosquilla oratoria from the Yellow Sea and East China Sea was examined with mitochondrial DNA control region sequences. In total, 394 samples were collected from 18 locations and 102 haplotypes were obtained. For the Yellow Sea, the overall nucleotide diversity and haplotype diversity were 0.006 9 and 0.946 8, respectively; while across all the East China Sea locations, the overall nucleotide diversity and haplotype diversity were 0.027 94 and 0.979 0, respectively. The results of AMOVA and pairwise F_(ST)(0.145 2, P 0.001) revealed moderate differentiation between the Yellow Sea and East China Sea populations of O. oratoria. However, neither the neighbor-joining tree nor haplotype network showed clades with geographic pattern, which indicated considerable gene flow was existed between the Yellow Sea and East China Sea, and supporting the high larval dispersal ability in this species. Mismatch distribution analysis and neutrality tests suggested that O. oratoria has undergone population expansion event, and the Pleistocene glacial cycles might have an impact on the historical demography of O. oratoria. The genetic information obtained in this study can provide useful information for sustainable improvements for capture fisheries management strategies.     

Mitochondrial DNA control region diversity and population structure of Pacific herring (Clupea pallasii) in the Yellow Sea and the Sea of Japan

To investigate the genetic variation and population structure of Pacific herring in the Yellow Sea and the genetic differentiation between the Yellow Sea and the Sea of Japan,fragments of 479-bp mitochondrial DNA control region were sequenced for 110 individuals collected from three different periods in the Yellow Sea and one locality in the Sea of Japan.High haplotype diversity and moderate nucleotide diversity were observed in Pacific herring.AMOVA and exact test of population differentiation showed no significant genetic differentiations among the three populations of the Yellow Sea and suggested the populations can be treated as a single panmictic stock in the Yellow Sea.However,a large and significant genetic differentiation(WST50.11;P50.00) was detected between the populations in the Yellow Sea and the Sea of Japan.The high sea water temperature in the Tsushima Strait was thought a barrier to block the gene exchange between populations of the two sea areas.The neutrality tests and mismatch distribution indicated recent population expansion in Pacific herring.     

Phytoplankton distribution and community structure in the East China Sea (ECS) continental shelf

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Effect of Food on Specific Dynamic Action(SDA) of Green and Red Types of Sea Cucumber(Apostichopus japonicus Selenka)

Specific dynamic action(SDA), the energy expended on all physiological processes that is associated with meal digestion and absorption, is strongly affected by food type. Effects of formulated diet(FMD), macroalgae(ALG) and sea mud(SMD) diets on the postprandial metabolic response of the green type and the red type of sea cucumber(Apostichopus japonicus) were studied in order to understand their feeding physiology. Food offered to A. japonicus was different in protein, lipid content and energy but not in meal mass. SDA of A. japonicus resulted in a 1.3–2.7 folds of increase in oxygen consumption that can persist for up to 4.8–31.7 h after digesting three different diets. In a given type of sea cucumber, the magnitude of SDA was the highest when fed with FMD, medium with ALG, and the lowest with SMD, which is probably due to the differences in diet components and protein contents. The red type sea cucumber showed greater SDA magnitude than the green type with each diet treatment, which might result from the difference in factorial scope between the two types of sea cucumber. However, the smallest magnitude or even no difference was observed between the two types of A. japonicus in SMD group, perhaps owing to the poor nutrition and digestion of sea mud.     

Characterization and Expression Analysis of a Complement Component Gene in Sea Cucumber (Apostichopus japonicus)

The complement system plays a crucial role in the innate immune system of animals. It can be activated by distinct yet overlapping classical, alternative and lectin pathways. In the alternative pathway, complement factor B(Bf) serves as the catalytic subunit of complement component 3(C3) convertase, which plays the central role among three activation pathways. In this study, the Bf gene in sea cucumber(Apostichopus japonicus), termed Aj Bf, was obtained by rapid amplification of c DNA ends(RACE). The full-length c DNA of Aj Bf was 3231 bp in length barring the poly(A) tail. It contained an open reading frame(ORF) of 2742 bp encoding 913 amino acids, a 105 bp 5'-UTR(5'-terminal untranslated region) and a 384 bp 3'-UTR. Aj Bf was a mosaic protein with six CCP(complement control protein) domains, a VWA(von Willebrand factor A) domain, and a serine protease domain. The deduced molecular weight of Aj Bf protein was 101 k Da. Quantitative real time PCR(q RT-PCR) analysis indicated that the expression level of Aj Bf in A. japonicus was obviously higher at larval stage than that at embryonic stage. Expression detection in different tissues showed that Aj Bf expressed higher in coelomocytes than in other four tissues. In addation, Aj Bf expression in different tissues was induced significantly after LPS or Poly I:C challenge. These results indicated that Aj Bf plays an important role in immune responses to pathogen infection.     

Genetic Variation Assessed with Microsatellites in Mass Selection Lines of the Pacific Oyster(Crassostrea gigas) in China

Four successive mass selection lines of the Pacific oyster, Crassostrea gigas, selected for faster growth in breeding programs in China were examined at ten polymorphic microsatellite loci to assess the level of allelic diversity and estimate the effective population size. These data were compared with those of their base population. The results showed that the genetic variation of the four generations were maintained at high levels with an average allelic richness of 18.8–20.6, and a mean expected heterozygosity of 0.902–0.921. They were not reduced compared with those of their base population. Estimated effective population sizes based on temporal variances in microsatellite frequencies were smaller to that of sex ratio-corrected broodstock count estimates. Using a relatively large number of broodstock and keeping an equal sex ratio in the broodstock each generation may have contributed to retaining the original genetic diversity and maintaining relatively large effective population size. The results obtained in this study showed that the genetic variation was not affected greatly by mass selection progress and high genetic variation still existed in the mass selection lines, suggesting that there is still potential for increasing the gains in future generations of C. gigas. The present study provided important information for future genetic improvement by selective breeding, and for the design of suitable management guidelines for genetic breeding of C. gigas.