Numerical responses of larval fishes to deepwater sewage disposal: a field assessment

This paper summarizes the results of a large-scale ‘beyond BACI’ designed assessment of the influences of effluent discharges from Sydney's deepwater sewage outfalls on assemblages of larval fishes. Larval fishes were sampled at three outfalls and at multiple control locations three times before and three times after the deepwater outfalls were commissioned. Sampling was stratified to account for time of year, oceanography and plume behaviour. Large numbers of fish larvae were caught near the outfalls and were thus exposed to sewage plumes. Results indicated that some larvae that normally occur at depth were displaced upwards by rising plumes, particularly when plumes surfaced. No predictable positive or negative numerical responses of larvae to outfalls were evident. Rather responses varied depending on the taxa, outfall and season. The large inherent level of natural spatial and temporal variability in abundances of larvae swamped the detection of possible impacts for many taxa. Future field based studies need to track larvae through plumes to assess changes in numbers, but also to test effects on the health and condition of larvae.     

The application of a Marine Biotic Index to different impact sources affecting soft-bottom benthic communities along European coasts

Following the European Water Framework Directive (2000/60/EC), the authors proposed, in a previous contribution, a Marine Biotic Coefficient (BC) to establish the ecological quality of soft-bottom benthos within European estuarine and coastal environments. The present study examines the application of the BC to the Atlantic (North Sea; Bay of Biscay; South of Spain) and Mediterranean (Spain and Greece) European coasts. The investigation assesses also the usefulness of the BC, in relation to different impact sources (e.g. drilling cuts with ester-based mud, submarine outfalls, heavy metals, industrial and mining wastes, jetties and sewerage works). The results obtained are consistent with those obtained using several methods and parameters, such as richness, diversity, evenness, Abundance-Biomass comparison plots and univariate and multivariate statistical analyses. The BC values provide a simple and clearly defined way to establish the ecological quality of soft-bottom benthos, complementary to the above mentioned methods.     

Organochlorine compound and trace metal contaminants in fish near Sydney's ocean outfalls

Sydney's inshore sewage outfalls were significant contributors to organochlorine contamination of inshore sedentary fish such as red morwong. Diversion of the sewage to deepwater outfalls has resulted in marked declines in the concentrations of these compounds in inshore red morwong. Apart from lead, however, similar trends did not occur for concentrations of trace metals. A wide variety of trace metals and organochlorine compounds were found in the flesh and/or livers of a variety of fish species caught at sites associated with the present deepwater ocean outfalls off Sydney. Fish caught at locations remote from Sydney also contained a wide variety of trace metals although organochlorine compounds were usually at lower levels. The mean contaminant levels in all species of offshore fish were generally found to be low when compared to the Australian National Food Authority's Maximum Residue Limit (NFA MRL). No fish analysed was found to have levels of an organochlorine compound above the MRL and no fish was found to have levels of zinc or lead above the MRL. When detected, fish with levels of a trace metal above the MRL appeared to be distributed throughout New South Wales, independent of the deepwater ocean outfalls off Sydney. There is no evidence as yet that the commissioning of the deepwater outfalls has led to an increase in levels of contaminants in the fish examined to the extent that they are of concern relative to the NFA MRLs.     

Baseline studies of delaware ocean outfall sites

A quarterly sampling programme was conducted during 1973–1974 off the coast of Delaware to provide an environmental baseline for two ocean sewage outfalls. Extensive physical measurements and water quality data, together with biological data (fish, invertebrates, bacteriological samples), were collected. Based on this research the design of sewage treatment for one of the outfalls was improved.     

The impact of the 1998 coral mortality on reef fish communities in the Seychelles

Coral reef fish communities in the Seychelles are highly diverse and remain less affected by the direct impacts of human activities than those on many other coral reefs in the Indian Ocean. These factors make them highly suitable for a detailed survey of the impacts of the 1998 mass coral mortality, which devastated the coral faunas of the region. Using underwater visual census (UVC) techniques. fish communities were sampled in three localities in the southern Seychelles and one locality in the northern (granitic) Seychelles. Initial surveys were undertaken from the latter site in 1997. Surveys were undertaken at all sites during the coral bleaching episode in 1998 prior to any major changes in the reef fish communities. Repeat surveys were undertaken in 1999 one year after the coral mortality. Over 250 fish species were sampled from 35 families. Results suggest that changes in the overall fish community structures are not great, despite massive changes in the benthic cover. Significant changes have been observed in a number of individual species. These include those most heavily dependent on live coral cover for shelter or sustenance. Future potential changes are discussed, and potential management interventions are considered.     

Efficacy of a multi-metric fish index as an analysis tool for the transitional fish component of the Water Framework Directive

The WFD has introduced an international commitment to assess the ecological status of transitional waters (TWs), within which fish communities are a key biological monitoring component. The Transitional Fish Classification Index (TFCI) outlined in this paper uses 10 ecological measures to analyse fish populations caught from various ecological niches using a variety of gear types within the Thames estuary. These reach and method-specific communities are then compared to a reference population created from a 'healthy' population from TWs of a similar type. The results indicate a progressive downstream increase the quality of fish communities, consistent with previous work; variation between methods can be accounted for by gear selectivity. Overall, the TFCI is an effective communication tool for converting ecological information into an easily understood format for managers, policy makers and the general public.     

Impacts of dredged material disposal on a tropical soft-bottom benthic assemblage

The soft-bottom benthic macrofauna in a spoil-ground of dredged material in Cleveland Bay, North Queensland, Australia, was studied to detect possible impacts of the disposal of sediments. The spatial distribution of the assemblage was studied in relation to the source of the impact at 28 stations on four occasions during 1998 and 1999. Additionally, environmental variables were measured on each occasion at each station. Macrobenthic assemblages inside the spoil-ground were different from assemblages outside the spoil ground only immediately after (15 days) the disposal of dredged material. Given the decrease in the abundance of organisms and number of species, it is suggested that this effect was due to direct burial of the macrobenthic assemblage inside the spoil-ground. Macrobenthic assemblages inside the spoil ground were not different from assemblages outside the spoil ground 3 months after dumping. These results suggest that the soft-bottom macrobenthic assemblages may respond quickly to the disturbance associated with the dumping of dredged material.     

Assessment of the macrobenthos in an Antarctic environment following recent pollution abatement

Quantative population data for shallow Antarctic soft-bottom communities in King Edward Cove, South Georgia indicate that although once grossly polluted by organic effluent and fuel oil from a whaling station, the fauna recovered within some eight years after contamination ceased. The results are discussed in relation to comparable studies in other polar and temperate areas.     

Amelioration of marine farming impact on the benthic environment using artificial reefs as biofilters

An in situ monitoring of the sediment characteristics and macrobenthic communities was undertaken at a marine fish culture site in subtropical waters of Hong Kong before and after the deployment of biofilters which were made of cement concrete artificial reef (AR) structures. According to the distance to the boundary of the fish cages, 6 points were selected as sampling stations: 2 at the fish cages, 2 near the boundary of the fish culture area, and 2 reference sites further away from the culture area. Bimonthly sediment samples were collected for analysis of silt-clay fraction (SCF), moisture content (MC), total organic carbon (TOC), total Kjeldahl nitrogen (TKN) and total phosphorus (TP). The macrobenthos (>0.5mm) present in the sediment were sorted, identified and enumerated. TOC, TKN and TP levels at the fish cage stations were consistently higher than those at the reference stations over the 1-year pre-AR and 2-year post-AR deployment monitoring period. The diversity of macrofauna was significantly reduced at the fish cage stations relative to the reference sites. The intermediary stations near the fish culture area showed a transitional state of disturbance. Over the 2-year post-AR deployment period, TOC, TKN and TP showed a decreasing trend at the fish cage and intermediary stations. More diverse macrofaunal communities were recorded at the fish cage stations, with species diversity H'increasing from 0-1 at the beginning of the AR deployment to H'>2 at the end of the study. The present results demonstrated that artificial reefs can improve the benthic abiotic environment and biotic conditions beneath fish rafts which are deteriorated due to farming activities.     

Impacts of gold mine waste disposal on deepwater fish in a pristine tropical marine system

Little is known about the impacts of mine waste disposal, including deep-sea tailings, on tropical marine environments and this study presents the first account of this impact on deepwater fish communities. The Lihir gold mine in Papua New Guinea has deposited both excavated overburden and processed tailings slurry into the coastal environment since 1997. The abundances of fish species and trace metal concentrations in their tissues were compared between sites adjacent to and away from the mine. In this study (1999-2002), 975 fish of 98 species were caught. Significantly fewer fish were caught close to the mine than in neighbouring regions; the highest numbers were in regions distant from the mine. The catch rates of nine of the 17 most abundant species were lowest, and in three species were highest, close to the mine. There appears to be limited contamination in fish tissues caused by trace metals disposed as mine waste. Although arsenic (several species) and mercury (one species) were found in concentrations above Australian food standards. However, as in the baseline (pre-mine) sampling, it appears they are accumulating these metals mostly from naturally-occurring sources rather than the mine waste.     

Assessing water stress in Mediterranean lotic systems: insights from an artificially intermittent river in Greece

Water stress in Mediterranean countries is the result of both variable and changing climatic conditions and widespread anthropogenic pressures. Evrotas, an intermittent river located in Southern Greece, was used as a case study to assess the impacts of water stress on Mediterranean lotic ecosystems. Based on hydrological analyses, it was revealed that during prolonged drought years, such as the summers of 2007 and 2008, the vast majority of the Evrotas riverbed was completely desiccated, primarily as a result of substantial water abstraction for irrigation. The effects of desiccation on the riverine ecosystem were evaluated using fish and macroinvertebrate assemblages according to the demands of the Water Framework Directive 2000/60/EC (WFD). Faunal responses to water stress were assessed through comparisons of assemblages attributes in perennial and intermittent reaches and pre-drought versus post-drought communities. Effects of hydrological disturbance on fish species richness, density, percentage composition and size structure were more pronounced in intermittent than in perennial sites. The most obvious and immediate impact was the elimination of populations in the intermittent reaches. However, upon flow resumption, the recolonisation from upstream perennial reaches began, thereby permitting partial re-establishment of the depleted fish communities. Nevertheless, the structural integrity of fish communities remained severely impacted and recovery was markedly slow. On the contrary, post-drought macroinvertebrate assemblages were not affected by summer droughts, and the recruitment processes were rapid after flow resumption. Our findings point to the necessity of establishing a distinction between naturally and artificially driven intermittent rivers. We, therefore, propose the introduction of an “artificially intermittent Mediterranean river” condition within the context of the WFD assessment applications.     

Projected entrainment of fish resulting from aggregate dredging

Previous research to assess impacts from aggregate dredging has focussed on infaunal species with few studies made of fish entrainment. Entrainment evidence from hydraulic dredging studies is reviewed to develop a sensitivity index for benthic fish. Environmental monitoring attendant with the granting of new licences in the Eastern Channel Region (ECR) in 2006 offers a unique opportunity to assess the effects of dredging upon fish. Projected theoretical fish entrainment rates are calculated based upon: abundance data from 4m beam trawl sampling of fish species over the period 2005-2008; sensitivity data; and dredging activity and footprint derived from Electronic monitoring System (EMS) data. Results have been compared with actual entrainment rates and also against summary results from independent analysis of the changes in fish population over the period 2005-2008 (Drabble, 2012). The case is made for entrainment surveys to form part of impact monitoring for marine aggregate dredging.     

Ecological Quality Status of the soft-bottom communities on the algerian coast: General patterns and diagnosis

Between 1995 and 2001, the soft-bottom communities along the 1180 km of the Algerian coast were sampled in nine gulfs and 12 harbours, providing a total of 655 samples. Eight macrozoobenthos-based biotic indices (S, N, H′, BQI, AMBI, BENTIX, BO2A and ITI) were selected to describe the general patterns of the coastal water quality status and to establish a quality diagnosis for the different zones subjected to anthropogenic pressure (e.g., harbour construction, industrial and urban pollution). Reference values were determined for each of the eight indices selected by analyzing the indices’ parameter distribution. The Ecological Quality Ratio (EQR) was estimated for each index, resulting in an EQR Mean Score and an EQR Bad Score. From these EQR, we defined an EQS for each sample. The agreement between these EQS was analysed using the Kappa method in order to propose a survey strategy for the Algerian coastal waters that would take into account the soft-bottom biological compartment. The results clearly indicate that high and good quality assessments are prevalent in the gulfs, while quality assessments in harbours vary greatly from bad to good. The effect of pollution observed in the harbours can be classified in two main groups, according to when they were constructed and their relative degree of openness to the sea, which permits better water circulation and probably dilutes the pollution.     

The influence of pearl oyster farming on reef fish abundance and diversity in Ahe,French Polynesia

Many cultured pearl farms are located in areas of the Pacific that have thriving, highly diverse fish communities but the impacts of farming on these communities are poorly understood. We studied the effects of pearl oyster farming on shore fish abundance and diversity in the lagoon of Ahe, French Polynesia by adapting roving diver census methods to the coral reef bommies of the lagoon and compared 16 sites with high pearl farming impact to others with no direct impact. Pearl farming has a slightly positive effect on reef fish abundance (N) and no significant impact on fish diversity (H) or community composition. This is important when considering the ecological sustainability of pearl farming in French Polynesia and suggests that a potential synergy between pearl farms and marine conservation should be further explored.     

岱海生物群落结构的演变及其对渔业发展的影响

岱海水生生物种类贫乏,生物量低,渔产力不高,优化岱海水体生物群落组成,引种耐低温、耐盐碱的水生植和藻类,改善水体环境调整鱼类种群结构是渔业发展的重要措施。     

Taxonomic level for assessing oil spill effects on soft-bottom sublittoral benthic communities

Infralittoral soft-bottom macrofauna abundance data collected during four-year surveys after the Aegean Sea (Galicia, Spain) and the Amoco Cadiz oil spill (Brittany, France) at a total of seven sites were analysed at three different taxonomic levels (species, genus, and family). The effects of the spills on macrofauna populations were very similar in the two areas. The post-spill time-courses of Shannon-Weaver diversity values were similar regardless of whether species-, genus- or family-level data were used. Non-metric multidimensional scaling identified well-defined groups of samples corresponding to different times post-spill, and again the results obtained were very similar regardless of the taxonomic level considered. In general, the use of genus- or family-level data did not lead to relevant information loss by comparison with species-level data. In view of these findings and those of previous studies of this type, we conclude that family-level data is generally sufficient for monitoring pollution effects in infralittoral soft-bottom environments.     

Verifying a biotope classification using benthic communities – An analysis towards the implementation of the European Marine Strategy Framework Directive

The HELCOM Red List biotopes project proposed a Baltic Sea wide classification consisting of six levels: The HELCOM Underwater biotopes/habitats classification system (HELCOM HUB). We present a case study from the south-western Baltic Sea where we tested the applicability of this system. More than 500 sampling stations were analyzed regarding macrozoobenthic communities and their linkage to environmental parameters. Based on the analyses of biotic and abiotic data, 21 groups were assigned to 13 biotopes of the classification. For some biotopes varying states of communities were recognized. Even though not all abiotic parameters are considered directly in the hierarchy of the classification in general, all soft-bottom communities could be allocated to a corresponding biotope. The application of the HELCOM HUB for the south-western Baltic Sea is feasible, in regard to the implementation of the European Marine Strategy Framework Directive as well as the Baltic Sea Action Plan.     

Amelioration of marine farming impact on the benthic environment using artificial reefs as biofilters

An in situ monitoring of the sediment characteristics and macrobenthic communities was undertaken at a marine fish culture site in subtropical waters of Hong Kong before and after the deployment of biofilters which were made of cement concrete artificial reef (AR) structures. According to the distance to the boundary of the fish cages, 6 points were selected as sampling stations: 2 at the fish cages, 2 near the boundary of the fish culture area, and 2 reference sites further away from the culture area. Bimonthly sediment samples were collected for analysis of silt-clay fraction (SCF), moisture content (MC), total organic carbon (TOC), total Kjeldahl nitrogen (TKN) and total phosphorus (TP). The macrobenthos (>0.5 mm) present in the sediment were sorted, identified and enumerated. TOC, TKN and TP levels at the fish cage stations were consistently higher than those at the reference stations over the 1-year pre-AR and 2-year post-AR deployment monitoring period. The diversity of macrofauna was significantly reduced at the fish cage stations relative to the reference sites. The intermediary stations near the fish culture area showed a transitional state of disturbance. Over the 2-year post-AR deployment period, TOC, TKN and TP showed a decreasing trend at the fish cage and intermediary stations. More diverse macrofaunal communities were recorded at the fish cage stations, with species diversity H′increasing from 0–1 at the beginning of the AR deployment to H′ > 2 at the end of the study. The present results demonstrated that artificial reefs can improve the benthic abiotic environment and biotic conditions beneath fish rafts which are deteriorated due to farming activities.     

Effects of industrial outfalls on tropical macrobenthic sediment communities in Reunion Island (Southwest Indian Ocean)

Temporal changes in the composition of soft bottom macrobenthic assemblages at Reunion Island (Southwest Indian Ocean) were studied in the context of a long-term environmental monitoring programme studying the impacts of effluents of industrial sugar cane refineries that are transferred to shallow and deep coastal environments by different pathways: surface discharge and deep underground injection. Seven stations (between 20 and 160 m depth) were surveyed between 1994 and 2003 on the industrial zone. One additional station was surveyed on a reference site. Spatio-temporal changes in the composition of macrobenthic communities were assessed using several diversity indices, ABC curves, MDS and associated ANOSIM tests and biotic indices. Among the 171 taxa recorded, polychaetes were dominant (89 species), followed by crustaceans and molluscs. The analysis of spatial changes in the composition of macrobenthos showed the existence of distinct benthic communities along the depth gradient. Temporal changes in macrobenthos composition were most prominent at the shallowest station. They mainly corresponded to the decline of several initially dominant taxa and the increase of the Eunicid polychaete Diopatra cuprea. This station further showed increasing macrofaunal abundance, biomass and sediment organic content over time, concomitant with decreasing sediment grain sizes. In deeper environments, temporal changes were much smaller. Macrofaunal abundance and species richness increased progressively, suggesting a moderate impact on benthic ecosystems resulting from slight enrichments due to effluents rich in organic matter. Our results highlight an original response to disturbance pattern involving opportunistic Eunicidae species (D. cuprea) not previously described. Moreover, they allow for the comparison of the impact on macrofauna caused by industrial effluents exported by two distinct and different pathways in a tropical coastal high-energy marine environment.     

Benthic input rates predict seagrass (Posidonia oceanica) fish farm-induced decline

Fish farms represent a growing source of anthropogenic disturbance to benthic communities, and efficient predictors of such impacts are urgently needed. We explored the effects of fish farm benthic organic and nutrient inputs on the population dynamics of a key seagrass species (Posidonia oceanica) in four Mediterranean deep meadows adjacent to sea bream and sea bass farms. We performed two annual plant censuses on permanent plots at increasing distance from farms and measured benthic sedimentation rates around plots. High shoot mortality rates were recorded near the cages, up to 20 times greater than at control sites. Recruitment rates increased in variability but could not compensate mortality, leading to rapid seagrass decline within the first 100 m from cages. Seagrass mortality increased with total sedimentation rates (K=0.55, p<0.0002), and with organic matter (K=0.50, p=0.001), total nitrogen (K=0.46, p=0.002) and total phosphorus (K=0.56, p<3.10(-5)) inputs. P. oceanica decline accelerated above a phosphorus loading threshold of 50mg m(-2)day(-1). Phosphorus benthic sedimentation rate seems a powerful predictor of seagrass mortality from fish farming. Coupling direct measurements of benthic sedimentation rates with dynamics of key benthic species is proposed as an efficient strategy to predict fish farm impacts to benthic communities.