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1.
Hydrophobic and electrostatic parameters in bacterial adhesion   总被引:5,自引:0,他引:5  
Recently it has been shown that the initial stages of bacterial adhesion to a model-surface of sulphated polystyrene can best be described using hydrophobic and electrostatic parameters. In the present study it is tested whether these parameters can generally be applied to predict bacterial adhesion by using (i) glass, as a model for hydrophilic and natural surfaces of silicates and oxides, (ii) polystyrene coated with proteins, as a model for a surface coated with an organic layer, and (iii) river Rhine sediment, as an example of a natural surface. Adhesion to glass was dominated by electrostatic interaction, whereas adhesion to polystyrene coated with various types of proteins depended on the surface characteristics of the bacteria and the type of protein. By relating Van der Waals interactions to hydrophobicity of the interacting species, the adhesion of bacteria to the various surfaces including the river Rhine sediments could be interpreted in terms of the DLVO-theory. It is therefore concluded that the conceptual principles of the DLVO-theory (interplay of Van der Waals and electrostatic interactions) are suitable to describe, at least qualitatively, the initial processes of bacterial adhesion to a wide range of surfaces.  相似文献   

2.
Current models describing the behavior for the triple friction pendulum (TFP) bearing are based on the assumption that the resultant force of the contact pressure acts at the center of each sliding surface. Accordingly, these models only rely on equilibrium in the horizontal direction to arrive at the equations describing its behavior. This is sufficient for most practical applications where certain constraints on the friction coefficient values apply as a direct consequence of equilibrium. This paper presents a revised model of behavior of the TFP bearing in which no assumptions are made on the location of the resultant forces at each sliding surface and no constraints on the values of the coefficient of friction are required, provided that all sliding surfaces are in full contact. To accomplish this, the number of degrees of freedom describing the behavior of the bearing is increased to include the location of the resultant force at each sliding surface and equations of moment equilibrium are introduced to relate these degrees of freedom to forces. Moreover, the inertia effects of each of the moving parts of the bearing are accounted for in the derivation of the equations describing its behavior. The model explicitly calculates the motion of each of the components of friction pendulum bearings so that any dependence of the coefficient of friction on the sliding velocity and temperature can be explicitly accounted for and calculations of heat flux and temperature increase at each sliding surface can be made. This paper presents (a) the development of the revised TFP bearing model, (b) the analytic solution for the force–displacement relations of two configurations of the TFP bearing, (c) a model that incorporates inertia effects of the TFP bearing components and other effects that are useful in advanced response history analysis, and (d) examples of implementation of the features of the presented model. Copyright © 2016 John Wiley & Sons, Ltd.  相似文献   

3.
Force identification is a type of system identification procedure which determines applied force from system responses; it has engineering applications where direct measurements of forces are not feasible. To identify the force, one can consider that the system model and its parameters are known, and then use the measured response of the system to determine the unknown forces. In contrast, postulating the system model and its parameter values is a difficult task, especially when considering a nonlinear system where the model poses many unknowns or inherent mathematical problems. In this research, a more direct way to identify the unknown force without knowing the model of the system is proposed. The approach, called the sum of weighted acceleration technique (SWAT), is a method that can predict input forces with measured linear and nonlinear structural responses. SWAT employs measured accelerations multiplied by effective or optimal weights to estimate the input force. Results using this method show that the force calculated using SWAT accurately predicts the force which excites a nonlinear structure response. The technique could be applied to both constrained and unconstrained structures.  相似文献   

4.
The distribution of seismic base shear demand among ductile flexural cantilever walls, comprising the lateral load resisting system of a multistorey building, is studied. It is shown that the base shear force demand depends on the sequence of hinge formation at the wall bases, and this in turn depends on the relative wall lengths. Hence, the routine elastic approach in which the shear forces are allocated per relative flexural rigidity or (when some consideration is given to plastic hinge formation) to moment capacity at the wall base, may appreciably underestimate the shear force demand on the walls, particularly the shorter (usually the more flexible) ones. A simple procedure yielding the results of ‘cyclic’ pushover analysis is proposed to predict the peak seismic wall forces for a given total base shear when plastification is confined to the wall base. The effects of plastic hinges developing at higher floors on (1) shear distribution among the walls and (2) the in‐plane floor forces are also considered. Two numerical examples are presented to demonstrate the main points made. Copyright © 2004 John Wiley & Sons, Ltd.  相似文献   

5.
Controlled rocking steel frames have been proposed as an efficient way to avoid the structural damage and residual deformations that are expected in conventional seismic force resisting systems. Although the base rocking response is intended to limit the force demands, higher mode effects can amplify member design forces, reducing the viability of the system. This paper suggests that seismic forces may be limited more effectively by providing multiple force‐limiting mechanisms. Two techniques are proposed: detailing one or more rocking joints above the base rocking joint and providing a self‐centring energy dissipative (SCED) brace at one or more levels. These concepts are applied to the design of an eight‐storey prototype structure and a shake table model at 30% scale. A simple numerical model that was used as a design tool is in good agreement with frequency characterization and low‐amplitude seismic tests of the shake table model, particularly when multiple force‐limiting mechanisms are active. These results suggest that the proposed mechanisms can enable better capacity design by reducing the variability of peak seismic force demands without causing excessive displacements. Similar results are expected for other systems that rely on a single location of concentrated nonlinearity to limit peak seismic loads. Copyright © 2012 John Wiley & Sons, Ltd.  相似文献   

6.
Shear wave velocity–depth information is required for predicting the ground motion response to earthquakes in areas where significant soil cover exists over firm bedrock. Rather than estimating this critical parameter, it can be reliably measured using a suite of surface (non-invasive) and downhole (invasive) seismic methods. Shear wave velocities from surface measurements can be obtained using SH refraction techniques. Array lengths as large as 1000 m and depth of penetration to 250 m have been achieved in some areas. High resolution shear wave reflection techniques utilizing the common midpoint method can delineate the overburden-bedrock surface as well as reflecting boundaries within the overburden. Reflection data can also be used to obtain direct estimates of fundamental site periods from shear wave reflections without the requirement of measuring average shear wave velocity and total thickness of unconsolidated overburden above the bedrock surface. Accurate measurements of vertical shear wave velocities can be obtained using a seismic cone penetrometer in soft sediments, or with a well-locked geophone array in a borehole. Examples from thick soil sites in Canada demonstrate the type of shear wave velocity information that can be obtained with these geophysical techniques, and show how these data can be used to provide a first look at predicted ground motion response for thick soil sites.  相似文献   

7.
Modern ethohydraulics is the study of the behavioral responses of swimming fish to flow fields. However, the exact drag forces experienced by fish remain poorly studied; this information is required to obtain a better understanding of the behavioral responses of fish and their current resistance strategies. We measured near-ground frontal drag forces on preserved individuals of three benthic fish species, round goby (Neogobius melanstomus), gudgeon (Gobio gobio) and bullhead (Cottus gobio), in a flow channel. The forces were compared to acoustic Doppler velocity (ADV) measurements and fish tracking data based on video observations of live fish in the flow channel. Overall, we observed drag coefficients (CD) of ∼10−3 at Reynolds numbers ∼105. The frontal drag forces acting on preserved fish with non-spread fins ranged from -1.96 mN*g-1 (force per fish wet weight, velocity 0.55 m*s-1) to 11.01 mN*g-1 (velocity 0.85 m*s-1). Spreading the fins strongly increased the drag forces for bullhead and round goby. In contrast, the drag forces were similar for gudgeon with spread fins and all fish with non-spread fins. Video tracking revealed no clear relationship between the position of the fish in the flow field and the forces experienced by the preserved fish at these positions. Collectively, these results suggest that i) the differences in frontal drag forces between species are small in homogenous flow, ii) individuals chose their position in the flow field based on factors other than the drag forces experienced, and iii) whether fins are spread or non-spread is an essential quality that modulates species-specific differences. The methodology and results of this study will enable integration of flow measurements, fish behavior and force measurements and inform ethohydraulics research. More advanced force measurements will lead to a detailed understanding of the current resistance strategies of benthic fish and improve the design of fish passes.  相似文献   

8.
We propose a new method for removing sea-surface multiples from marine seismic reflection data in which, in essence, the reflection response of the earth, referred to a plane just above the sea-floor, is computed as the ratio of the plane-wave components of the upgoing wave and the downgoing wave. Using source measurements of the wavefield made during data acquisition, three problems associated with earlier work are solved: (i) the method accommodates source arrays, rather than point sources; (ii) the incident field is removed without simultaneously removing part of the scattered field; and (iii) the minimum-energy criterion to find a wavelet is eliminated. Pressure measurements are made in a horizontal plane in the water. The source can be a conventional array of airguns, but must have both in-line and cross-line symmetry, and its wavefield must be measured and be repeatable from shot to shot. The problem is formulated for multiple shots in a two-dimensional configuration for each receiver, and for multiple receivers in a two-dimensional configuration for each shot. The scattered field is obtained from the measurements by subtracting the incident field, known from measurements at the source. The scattered field response to a single incident plane wave at a single receiver is obtained by transforming the common-receiver gather to the frequency–wavenumber domain, and a single component of this response is obtained by Fourier transforming over all receiver coordinates. Each scattered field component is separated into an upgoing wave and a downgoing wave using the zero-pressure condition at the water-surface. The upgoing wave may then be expressed as a reflection coefficient multiplied by the incident downgoing wave plus a sum of scattered downgoing plane waves, each multiplied by the corresponding reflection coefficient. Keeping the upgoing scattered wave fixed, and using all possible incident plane waves for a given frequency, yields a set of linear simultaneous equations for the reflection coefficients which are solved for each plane wave and for each frequency. To create the shot records that would have been measured if the sea-surface had been absent, each reflection coefficient is multiplied by complex amplitude and phase factors, for source and receiver terms, before the five-dimensional Fourier transformation back to the space–time domain.  相似文献   

9.
Adhesion of two marine bacteria Shewanella sp. strain T1 and Pseudoalteromonas sp. strain T8, on differently terminated alkanethiolate self-assembled monolayers on gold was investigated. The selected model surfaces—terminated by CH3, OH, NH2, COOH, OH-terminated oligo(ethylene glycol), and methyl-terminated oligo(ethylene glycol)—are characterized by contact angle measurement using water, methylene iodide, 1-bromonaphthalene, and formamide. Surface free energies were calculated. Cell counting of the two bacterial strains on the model surfaces after different times revealed differences between the two strains by at least one order of magnitude. For the different surfaces, the bacteria showed comparably small selectivity. Atomic force microscopy images of adhered bacteria showed very different fingerprints on the different surfaces. Electronic supplementary material  Supplementary material is available in the online version of this article at and is accessible to authorized users.  相似文献   

10.
The principal causes of earthquake-induced ground deformation are identified and their interaction with underground infrastructure, primarily pipelines and conduits, is described. The coupled forces normal and parallel to underground pipelines arising from earthquake-induced ground movement are evaluated, including a review of measured stresses on pipe surfaces during large-scale testing, evaluation of frictional forces related to soil-pipe interaction, and the resolution of interaction forces along and across pipelines. Methods for characterizing soil reaction to pipe lateral and vertical movements are presented. The maximum downward pipe force is only about one-third the maximum force determined with conventional bearing capacity equations, thus requiring changes in current analytical and design practice. The analytical results for pipeline response to strike-slip and normal fault rupture are shown to compare favorably with the results of both large-scale and centrifuge tests of soil-pipeline interaction simulating these types of severe ground deformation.  相似文献   

11.
This paper provides a review of bacterial transport experiments conducted by a multiinvestigator, multiinstitution, multidisciplinary team of researchers under the auspices of the U.S. Department of Energy (DOE). The experiments were conducted during the time period 1999-2001 at a field site near the town of Oyster, Virginia known as the South Oyster Site, and included four major experimental campaigns aimed at understanding and quantifying bacterial transport in the subsurface environment. Several key elements of the research are discussed here: (1) quantification of bacterial transport in physically, chemically, and biologically heterogeneous aquifers, (2) evaluation of the efficacy of conventional colloid filtration theory, (3) scale effects in bacterial transport, (4) development of new methods for microbial enumeration and screening for low adhesion strains, (5) application of novel hydrogeophysical techniques for aquifer characterization, and (6) experiences regarding management of a large field research effort. Lessons learned are summarized in each of these areas. The body of literature resulting from South Oyster Site research has been widely cited and continues to influence research into the controls exerted by aquifer heterogeneity on reactive transport (including microbial transport). It also served as a model (and provided valuable experience) for subsequent and ongoing highly-instrumented field research efforts conducted by DOE-sponsored investigators.  相似文献   

12.
A scheme is proposed to calculate the effect of torsion on each lateral load resisting element of asymmetrical buildings in the context of the response spectrum technique. The scheme consists of: (i) Obtain the modal shear and torque on the building by the response spectrum technique, (ii) Compute the total modal shear forces on each frame by resolving the modal shear and torque on the building according to principles of structural mechanics. The shears on each frame due to the lateral load effect and torsional effect are combined algebraically, (iii) Obtain the total shear force on each frame by combining the total modal shears on that frame in a root sum square manner. Since the proper phase relationship between the lateral load effect and torsional effect is accounted for on a modal basis, it is believed that the proposed scheme provides a more realistic load estimate on the frames than the conventional approach. An example of a simplified mono-symmetrical frame structure is chosen to illustrate the accuracy of the proposed scheme, using dynamic time-history analysis as a standard for comparison.  相似文献   

13.
应用极限平衡拟静力方法进行地震边坡稳定性分析时,对粘聚力较小、内摩擦角较大的浅层滑坡而言,随着地震水平设计加速度值的增大,其临界滑动面基本保持不变;然而,对粘聚力较大而摩擦角较小的深层滑坡而言,随着地震水平设计加速度值的增大,临界滑动面越深、越宽,会呈现出发散现象,选取两个典型的均质土坡,设计了由浅及深的多条滑动面,分别采用极限平衡瑞典方法进行了相应安全系数的计算,同时比较了自重、地震力、粘聚力在抗滑力矩以及滑动力矩中所占比重的变动情况。研究发现:对于浅层滑坡,随着滑动面由浅及深的变化,粘聚力产生的抗滑力矩所占百分比下降幅度较大,自重产生的滑动力矩与总体抗滑力矩的比值下降幅度较大;而对于深层滑坡,随着滑动面由浅及深变化,自重产生的抗滑力矩所占百分比增长的幅度较大,地震力产生的滑动力矩与总体抗滑力矩比值增长幅度也较大。  相似文献   

14.
Accelerations and displacements due to dynamic excitation by simulated traffic consisting of two trucks, were measured on the deck and tower of the Alamillo cable-stayed bridge. Also the dynamic testing program included the measurement with accelerometers of the free-damped vibration of the 26 cables achieved by means of quick-releasing force. From these measurements it was possible to obtain the dynamic parameters of the bridge (natural frequencies and damping ratios) and the real forces in the cables. In the paper, only the tests, results and conclusions related to dynamic parameters of the bridge are presented. The objective of the dynamic tests herein described was to validate the mathematical modelling and the wind-tunnel models used in the dynamic analysis of the bridge in front of traffic and wind-forces. As the agreement between dynamic parameters of the real bridge and theoretical and scaled models was very satisfactory, the correct dynamic behaviour of the bridge in response to traffic and wind (vortex shedding, flutter, etc.) can be deduced jointly with the correct alignment and expected internal forces in the permanent state in tower and deck.  相似文献   

15.
The principles of operation and force–displacement relationships of three novel spherical sliding isolation bearings are developed in this paper. These bearings are completely passive devices, yet exhibit adaptive stiffness and adaptive damping. That is, the stiffness and damping change to predictable values at calculable and controllable displacement amplitudes. The primary benefit of adaptive behavior is that a given isolation system can be separately optimized for multiple performance objectives and/or multiple levels of ground shaking. With the devices presented here, this is accomplished using technology that is inherently no more complex than what is currently used by the civil engineering profession. The internal construction consists of various concave surfaces and behavior is dictated by the different combinations of surfaces upon which sliding can occur over the course of motion. As the surfaces upon which sliding occurs change, the stiffness and effective friction change accordingly. A methodology is presented for determining which surfaces are active at any given time based on the effective radius of curvature, coefficient of friction and displacement capacity of each sliding surface. The force–displacement relationships and relevant parameters of interest are subsequently derived based on the first principles. Copyright © 2007 John Wiley & Sons, Ltd.  相似文献   

16.
海洋中尺度涡与内波的地震图像   总被引:10,自引:5,他引:5       下载免费PDF全文
海洋反射地震通常用于调查、研究海底地质构造,勘探油气与天然气水合物资源.近期研究表明多道反射地震方法也可以对水柱的热盐细结构成像.中尺度涡与内波是重要的物理海洋现象,但是常规的物理海洋调查是在间隔若干公里的离散测站上进行的,水平分辨率较低,因此对中尺度涡的结构与内波的横向分布了解较差.本文利用在大西洋东部、南海采集的地震数据给出了低频反射地震可以对中尺度涡与内波清晰成像的新的证据.反射地震方法较传统海洋观测手段,具有明显的优势,主要体现在高的水平分辨率和短时间内对整个海水剖面进行成像方面.从地震剖面上,能够清楚地观测到中尺度涡、内波造成的反射特征变化,从而有助于改进对能量在不同尺度的海水运动之间传递过程的认识.  相似文献   

17.
In this paper,the characteristics of forces in active control systems connected to adjacent levels of a building are analyzed.The following characteristics are observed:(1) active control can provide signifi cantly superior supplemental damping to a building,but causes a small frequency shift;(2) the linear quadratic regulator(LQR)-based control force is composed of an elastic restoring force component and a damping force component,where the damping force is almost identical to the total control force,howev...  相似文献   

18.
An Erratum has been published for this article in Earthquake Engng. Struct. Dyn. 2004; 33:1429. Based on structural dynamics theory, the modal pushover analysis (MPA) procedure retains the conceptual simplicity of current procedures with invariant force distribution, now common in structural engineering practice. The MPA procedure for estimating seismic demands is extended to unsymmetric‐plan buildings. In the MPA procedure, the seismic demand due to individual terms in the modal expansion of the effective earthquake forces is determined by non‐linear static analysis using the inertia force distribution for each mode, which for unsymmetric buildings includes two lateral forces and torque at each floor level. These ‘modal’ demands due to the first few terms of the modal expansion are then combined by the CQC rule to obtain an estimate of the total seismic demand for inelastic systems. When applied to elastic systems, the MPA procedure is equivalent to standard response spectrum analysis (RSA). The MPA estimates of seismic demand for torsionally‐stiff and torsionally‐flexible unsymmetric systems are shown to be similarly accurate as they are for the symmetric building; however, the results deteriorate for a torsionally‐similarly‐stiff unsymmetric‐plan system and the ground motion considered because (a) elastic modes are strongly coupled, and (b) roof displacement is underestimated by the CQC modal combination rule (which would also limit accuracy of RSA for linearly elastic systems). Copyright © 2004 John Wiley & Sons, Ltd.  相似文献   

19.
针对20多个现役结构,以基于力的抗震设计方法所求基底剪力与直接基于位移的抗震设计方法所求基底剪力相等为原则,回归给出了直接基于位移的抗震设计中基底剪力的调整系数。在此基础上,通过进一步分析,提出了直接基于位移可靠度的抗震设计中层间目标位移代表值的确定方法。最后,总结给出了直接基于位移可靠度的抗震设计步骤。  相似文献   

20.
This paper is focused on the research of seismic performance of cylindrical latticed-intersected-three-dimensional-beam-system (LITDBS) reticulated mega-structure with single-layer latticed-intersected-cylindrical-shell (LICS) substructures by time history method accounting for geometrical nonlinearity. The distribution laws of the dynamic internal forces and those of the ratios of the dynamic to static internal forces of members in this innovative structure are first studied. The behaviors of the structure under different multi-component seismic actions are analyzed, and the reasonable components of the seismic action that should be considered in design are thus obtained. Then taking the maximum dynamic internal force of the structure as the index, a series of parametric analyses are carried out, and the suitable values of several key form parameters are recommended. Additionally, the results by the response spectrum method with those by the time history method are compared, which indicates that the former is not on the safe side. Therefore, the mode-superposition response spectrum method can only be used to do preliminary seismic design of this kind of structure, and the results must be checked by the time history method at the end.  相似文献   

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