Microbial iron reduction during passive in situ remediation of an acidic mine pit lake mesocosm

Ferric iron reduction was studied in a pilot-scale enclosure experiment for passive biological remediation of an acidic mine pit lake in Lusatia, Germany. The metabolic properties of prokaryotes involved in Fe(III) reduction may be important for the outcome of biological remediation, as chemolithotrophic Fe(III) reduction can counteract the desired pH increase, but heterotrophic Fe(III) reduction will provide the necessary Fe(II) for precipitation of sulfide minerals following sulfate reduction. Therefore, vertical profiles of sediment parameters related to iron and sulfur cycling were determined in conjunction with viable counts of different ferric iron-reducing micro-organisms using selective media. Findings were compared to an untreated reference site. The addition of organic matter stimulated ferric iron reduction and sulfate reduction in the enclosure and led to elevated pH and accumulations of ferrous iron and reduced sulfur compounds. Numbers of neutrophilic heterotrophic Fe(III) reducers increased during treatment, those of acidophilic heterotrophic Fe(III) reducers remained similar, and those of acidophilic chemolithotrophic Fe(III) reducers decreased. Zones of ferric iron-reducing activity corresponded well with microbial depth profiles; however, viable counts of neutrophilic or acid-tolerant Fe(III) reducers must have been underestimated based on the corresponding observed activity levels. Ferric iron reduction by chemolithotrophic acidophiles seemed to be of minor importance, so a lowering of pH values due to Fe(III) reducing activity is unlikely.     

Temporal and spatial characteristics of dissolved organic carbon in the Wujiang River,Southwest China

River systems play an important role in the global carbon cycle. Rivers transport carbon to the ocean and also affect the carbon cycle in the coastal ocean. The flux from land to the ocean is thought to be a very important part of the land carbon budget. To investigate the effect of dam-building on dissolved organic carbon(DOC)in rivers, three reservoirs of different trophic states in the Wujiang basin, Guizhou Province, were sampled twice per month between May 2011 and May 2012. Temporal and spatial distributions of DOC in the reservoirs and their released waters were studied. It was found that different factors controlled DOC in river water, reservoir water, and released water. DOC in the rivers tended to be affected by primary production. For reservoirs, the main controlling factors of DOC concentration varied by trophic state. For the mesotrophic Hongjiadu Reservoir, the effect of primary production on DOC concentration was obvious. For the eutrophic Dongfengdu Reservoir and the hypereutrophic Wujiangdu Reservoir, primary production was not significant and DOC came instead from soil and plant litter.     

基于MODIS数据的全国144个重点湖库营养状态监测:以2018年夏季为例

随着经济社会的快速发展和进步,我国湖库水体富营养化情况越来越严重.卫星遥感在水体营养状态监测方面具有重要潜力,但基于卫星遥感的全国范围内湖库水体营养状态监测和分析方面还鲜有研究.本文基于2018夏季的MO-DIS卫星遥感数据生产FUI指数产品,构建基于FUI水色指数的湖库营养状态评价方法,监测全国范围内144个重点湖库...     

新疆博斯腾湖浮游细菌丰度对富营养化及咸化的响应

本文以地处干旱地区我国最大的内陆淡水湖——博斯腾湖为研究对象,于2010年6月,在博斯腾湖大小湖区共选取23个采样点,采用表面荧光显微镜直接计数法( FDC)研究了水体中浮游细菌丰度的空间分布规律及其与环境因子的关系.结果表明:博斯腾湖水体中总氮(TN)浓度在0.26-1.45 mg/L之间变动(均值0.91 mg/L...     

Microbial colonization and decomposition of invasive and native leaf litter in the littoral zone of lakes of different trophic state

Riparian invasion by non-native trees may lead to changes in the quality of leaf litter inputs into freshwater ecosystems. Different plant species may affect the community of decomposers and the rate of litter decay in different ways. We studied the microbial colonization and decomposition of leaf litter of the invasive to Lithuania Acer negundo and native Alnus glutinosa during 64-day litterbag experiments in the littoral zones of mesotrophic and eutrophic lakes. The decomposition of A. negundo leaf litter proceeded faster than that of A. glutinosa irrespective of differences in the trophic conditions of the lakes. The amount of terrestrial and cellulose-degrading fungi (during the initial period) and bacterial numbers (during the experiment) were higher on A. negundo leaves than on A. glutinosa in both lakes. Differences in the assemblages of aquatic fungi colonizing the leaves of both types might be one of the reasons causing variation in their decay. The trophic conditions of the lakes did not significantly determine the extent of differences in decomposition rates between the two leaf species, but affected the microbial decomposers. The sporulation rate and diversity of aquatic fungi, especially on A. glutinosa leaves, was higher in the mesotrophic lake than in the eutrophic lake, while heterotrophic bacteria were more numerous on the leaves in the eutrophic lake. Generally, differences in the colonization dynamics of heterotrophs and the faster decay of A. negundo litter than of A. glutinosa suggest that the replacement of native riparian species such as the dominating A. glutinosa by invasive A. negundo may cause changes of organic matter processing in the littoral zones of lakes.     

Seasonal changes of microbial populations in the sediments of the basins of Lugano and Agno

This research was carried out with the aim to explore the heterotrophic microbial population of two sediments in different oxic conditions of the Lake of Lugano (Lago di Lugano). The values of the viable bacterial counts found in our sediment samples were typical for an eutrophic lake.The increase in the proportion of anaerobic to aerobic bacteria at 30 °C observed in the sediment samples of Agno may reflect the depletion of oxygen concentrations in the water column.The generaAeromonas, Pseudomonas, Bacillus andClostridium, as well as strains belonging to the Coryneform-group, represented the major taxonomic groups of heterotrophic bacterial communities in the water-sediment interface.     

Periphyton and sphagnicolous protists of dystrophic bog lakes (Brandenburg, Germany): I. Annual cycles, distribution and comparison to other lakes

The periphyton of acid, dystrophic bogs was investigated. Glass-slides were exposed in four different bog lakes about 100 km north of Berlin, Germany, in order to examine the species composition and the annual cycle of the protist community. The annual cycles of the periphyton in bog lakes were comparable to those in other mesotrophic lakes: During the winter months small heterotrophic flagellates (HF) dominated the periphyton community. Besides that bacterivorous ciliates were present as typical pioneer species. At the end of the ice covering, almost exclusively unicellular green algae colonized the slides. In May high abundances of ciliates and choanoflagellates were observed. At the same time, a maximum of species was reached. From August to October peritrich ciliates significantly contributed to the periphyton. Their number decreased when early frosts occurred.

The four investigated bog lakes exhibit very similar species compositions, almost all of the species were present in each of the four lakes. Differences were only distinct concerning the dominance of some protistan groups. The largest deflections, however, occurred not between two lakes, but between two habitats within one lake: The upper 30 cm of the Sphagnum mat comprise two very different protistan communities, determined by a strong vertical zoning. Due to the different light and nutrient conditions, the slides of the upper region exhibited a denser colonization, mainly consisting of autotrophic cryptomonads and vagile ciliates. In contrast to this, the slides of the deeper region were mainly colonized by heterotrophic flagellates and sessile peritrich ciliates.     

Fischwachstum bei Oligo-, Meso- und Eutrophie des Bodensees

Recent growth of 14 fish taxa of Lake Constance has been studied. In the mesotrophic basin summer-pelagic species opposite to the others appear to grow faster now than during earlier oligotrophic status. From trends of growth, feeding and yield (table) it is concluded that eutrophication has favoured planktivores.—In the eutrophic lower basin only coregonids grow slower than in the mesotrophic part of the lake. Factors that have caused growth differences are thought to be food density and water temperature (through changed fish distribution and different shape of basin).     

不同污染程度湖泊底栖动物群落结构及多样性比较

首次对湖北梁子湖水系污染程度不同(中营养型、中富营养型、富营养型)的4个湖泊的底栖动物群落结构和物种多样性进行了周年研究,结果表明,底栖动物种类数、物种多样性与湖泊受污染程度呈负相关系,密度与污染程度大体上呈正相关．还讨论了底栖动物环境指示种生态特性,研究表明中国长足摇蚊的密度与水体营养水平呈正相关,软体动物种类数与湖泊污染程度呈负相关．通过对软体动物、寡毛类及摇蚊类密度和生物量在不同湖泊之间的差异的分析,表明湖泊水体污染导致了底栖动物多样性明显降低．     

The dynamics of phytoplankton, bacteria and heterotrophic flagellates at two Banks near Magdeburg in the River Elbe (Germany)

The main objectives of this study were to describe the seasonal standing stock dynamics of phytoplankton, bacterioplankton and heterotrophic flagellates in the highly eutrophic River Elbe (Germany), and to compare the seasonal patterns observed with other streams. Emphasis was placed on examining and assessing abiotic and biotic controlling factors influencing the structure and dynamics of the riverine plankton. All the physico-chemical and biological parameters determined were within the range or somewhat higher (in the case of phytoplankton abundance and biomass) than reported for other large streams. The underwater light conditions resulting from atypically short phytoplankton growth periods of about 6 months per year and the low phytoplankton carbon to chl a ratio of 23 were identified as a major limiting factor for phytoplankton development in the River Elbe. The seasonal distribution pattern of bacterioplankton indicated probable tight trophodynamical coupling both with phytoplankton and with heterotrophic flagellates, whereas heterotrophic flagellates showed a more trophic link with bacterial densities. Although approximately constant DOC and DON levels throughout the year sustained bacterial growth rates, during the phytoplankton growing season an increase of bacterial standing stocks was observed. Although the left-bank sampling site of the Elbe is strongly influenced by the tributaries Mulde and Saale containing higher concentrations of chloride, nitrogen nutrients, heavy metals and organic pollutants, no clear differences were observed between the two sides of the river concerning the biological parameters measured. Possible reasons and the slightly higher phytoplankton abundance and diversity at the right bank are discussed.     

洱海富营养化影响因素分析

水质富营养化评价表明,洱海现属中营养水平,根据全年平均状态下的某些单项指标评分或近年来某些时段的全湖营养类型综合评分,将洱海评价为从中营养状态向营养营养状态过渡型的湖泊可能更为适宜。与历史资料相比,８０年代至今富营养化进程料快,主要营养盐守趋势。     

白洋淀超微真核藻的空间分布特征及关键影响因子

超微真核藻个体微小、比表面积大,具有高效的碳吸收速率,对水体初级生产力具有重要的贡献.目前对淡水超微真核藻的认知还非常有限.于2017年5月对白洋淀进行采样调查,结合流式细胞术和高通量测序技术探究了白洋淀超微真核藻群落结构的空间分布特征及关键环境影响因子.结果表明,白洋淀超微真核藻的平均丰度为7.59×104cells/ml,且随着营养水平的升高呈现先增加后降低的变化趋势.回归分析表明,超微真核藻丰度在中营养水平水域主要受溶解性总磷和TN/TP影响,在富营养水域主要与盐度有关.测序比对结果表明在纲水平上白洋淀超微真核藻主要以绿藻纲Chlorophyceae、硅藻纲Bacillariophyceae、中心硅藻纲Coscinodiscophyceae、金藻纲Chrysophyceae为主,不同区域差异不大.但在操作分类单元(Operation Taxonomy Units,OTU)水平上,超微真核藻群落结构在白洋淀不同营养状态湖区存在显著差异,中营养水域主要类群为栅列藻科Scenedesmaceae,近囊胞藻属Paraphysomona sp.,定鞭藻纲Haptophyceae和甲藻纲Dinophyceae为主,而富营养水域主要类群为红球藻科Haematococcaceae,金藻纲的Chromulinale sp.和Chrysophycea sp..Bioenv分析表明,对超微真核藻群落组成影响最大的环境因子是溶解性总氮、溶解性总磷、TN/TP、硝态氮、溶解氧.本研究表明超微真核藻的群落结构存在较明显的环境异质性,对白洋淀水体富营养化状态有很好的指示.     

Drivers of phytoplankton,bacterioplankton, and zooplankton carbon biomass in tropical hydroelectric reservoirs

Studies of carbon sources in plankton communities are important because carbon content has become the main currency used in functional studies of aquatic ecosystems. We evaluated the contribution to the total organic carbon pool from different plankton communities (phytoplankton, bacterioplankton, and zooplankton – C-biota) and its drivers in eight tropical hydroelectric reservoirs with different trophic and hydrological status and different physical features. Our systems were separated into three groups based on trophic status and water residence time: (i) mesotrophic with low residence time (ML); (ii) mesotrophic with high residence time (MH); and (iii) eutrophic with low residence time (EL). Our hypothesis that reservoirs with low water residence times and low nutrient concentrations would show the lowest C-biota was supported. Phytoplankton carbon (C-phy) showed the highest concentrations in the EL, followed by MH and ML systems. The EL group also showed significantly higher zooplankton carbon (C-zoo). No significant difference was observed for bacteria carbon (C-bac) among the three system groups. In addition to trophic status and water residence time, regression analyses revealed that water temperature, light, pH, and dissolved organic carbon concentrations were the main drivers of plankton communities in these large tropical hydroelectric reservoirs.     

原生动物纤毛虫对太湖梅梁湾水质富营养化的响应

１９９３年４月－１９９４年１月研究了太湖梅梁湾湖区纤毛虫的群落分布,共检出７目１９属,根据ＴＳＩ指标,该湖区可分为富营养型和中营养型水域,在富营养型水域中分布着特有的纤毛虫种类。     

水库浮游细菌生产和呼吸过程代谢内外源有机碳研究

全球气候变化和人类活动的加剧影响水生生态系统中溶解性有机碳(DOC)的种类和浓度.浮游细菌生产和呼吸过程对碳源变化的响应,可能影响水体乃至区域的碳循环特征.本研究选取了南京市11座中小型水库,利用碳稳定同位素技术和双端元混合模型定量分析浮游细菌在生产和呼吸代谢过程中对内外碳源的利用.依据DOC中内外碳源的比例,将11座...     

Untersuchungen über den Schwermetallhaushalt in Seen

In the mesotrophic Lake of Lucerne (Bay of Horw) and in the eutrophic Lake of Alpnach concentration and sedimentation of iron, copper, zinc, cadmium and lead were investigated as a function of time and depth, and compared with a series of physical and chemical parameters. A metal balance was made for the Lake of Alpnach. A model for the distribution of the metals between biomass, allochthonous material and dissolved phase was employed. Phenomenology and model lead to the following conclusions: the sedimentation of the metals is dominated by allochthonous particles, the biogenic particularization is partially reversed by lake internal decomposition processes and the trace metals reaching the sediment are partially redissolved.     

Paleolimnological evidence of limnetic nutrient concentration equilibrium in a shallow, macrophyte-dominated lake

: Paleolimnological techniques were used to infer long-term changes in trophic status of Lake Opinicon (Ontario), a mesotrophic, shallow, macrophyte-dominated lake, and to assess whether these patterns support the hypothesis of 'alternative equilibria' proposed for shallow lake systems. Analysis of the diatom assemblages indicated cold, oligotrophic to mesotrophic, alkaline conditions during the period shortly following deglaciation (>11000 yr BP). With the establishment of an open spruce-woodland (ca. 11000 yr BP), benthic diatoms dominated, indicating shallow waters and the presence of macrophytes. Pieces of aquatic macrophytes also became common in the core. An increase in lake productivity to mesotrophic levels (diatom-inferred [TP] of 23 7g/L) occurred following the transition to a deciduous forest in the catchment ca. 7500 yr BP. Paleolimnological data did not indicate any significant change in limnetic nutrient concentrations resulting from the hemlock decline (ca. 4800 yr BP) or from extensive flooding with the construction of the Rideau Canal (1828-1832). Modest nutrient enrichment of the lake has occurred more recently with increasing agricultural and residential/recreational activities, with a diatom-inferred [TP] increase to 32 7g/L. However, compared to other, similarly-disturbed deep lakes in the area, Lake Opinicon showed much smaller changes in lakewater conditions, still maintaining a clear-water, mesotrophic state and a large macrophyte community.¶The apparent inertia of Lake Opinicon to changes in trophic status, as compared to other deep, near-by, stratifying lakes in southeastern Ontario, supports the 'alternative equilibria' hypothesis. Paleolimnological evidence suggests that Lake Opinicon has been in a clear-water, equilibrium state dominated by macrophytes since early in its development, and has displayed hysteresis to nutrient enrichment. These results may provide lake managers with important information for effective management strategies of shallow water systems in southeastern Ontario.     

Effect of eutrophication on species composition and growth of freshwater mussels(Mollusca,Unionidae) in Lake Hallwil (Aargau,Switzerland)

Species composition, relative abundance and life history of unionid mussels are compared between 1982–86 and 1915–19 in Lake Hallwil and the outflowing brook. The recent samples of unionid mussels were collected by divers, whereas the older ones were from a shell collection. The motivation for the comparison was that the trophic degree of the lake has changed since the beginning of the century from mesotrophic to highly eutrophic. The effects of this increased trophic degree of the lake on the life cycle of unionid mussels is discussed. Predictions are made about species composition and life history in the context of the ongoing lake restoration by the authorities.     

The significance of transparent exopolymeric particles in the vertical distribution of bacteria and heterotrophic nanoflagellates in Lake Pavin

The abundance, size distribution, and bacterial colonization of Transparent Exopolymeric Particles (TEP) were examined in two consecutive years during the spring diatom development throughout the water column of the deep meromictic Lake Pavin, France. TEP concentrations ranged from 1.9 to 13.4 × 10⁵ particles l⁻¹ and their distribution and size spectra indicated that these particles are the main factor in governing the transport of diatoms to the deep hypolimnion of the lake. The majority of TEP was colonized by bacteria that constituted 0.4–8.9% of total DAPI-stained bacteria. The intensity of bacterial colonization was strongly related to temperature and decreased with particle size. A more important colonization of small particles in the hypolimnion during thermal stratification suggested that bacterial colonisation also increased with the age of the particle. The abundance of heterotrophic nanoflagellates (HNF) was more significantly related to the density of particles than to the density of total bacteria and the intensity of bacterial colonization of TEP. Our results therefore suggest that TEP are a more important factor for HNF development than attached and free bacteria. We conclude that TEP are involved not only in sedimentation processes but also in the dynamics of bacteria and protozoa in freshwater pelagic environments.     

Lacustrine environment responses to human activities in the past 300 years in Longgan Lake catchment,southeast China

The chronology of a gravity core sediment from Longgan Lake center was defined by²¹⁰Pb, combining with historic events recorded by document and sediment. The relationship between vegetation, soil erosion and lake nutrient state was discussed based on pollen, magnetic parameters, diatoms, phosphorus and pigments. The results show that the lake has undergone twice obvious transformations from oligotrophic to mesotrophic condition. Two eutrophications occurring at about 1768AD and the beginning of the 20th century respectively were related to external nutrient loading increase resulting from the enhanced human activities in the lake catchment. It is probable that strength of human actions in historic periods was influenced more or less by climatic changes. The lake eutrophication presented a more serious tendency because of the wide use of chemical fertilizer, reclamation of wetland and wetland vegetation destruction around the lake in the last 40 years.