Metabolism of polychaete Neanthes japonica Izuka: relations to temperature, salinity and body weight

Polychaete Neanthesjaponica is a species geographically specific in China and Japan with important scientific implication and commercial value. In this study, the relations of body weight, salinity and temperature to oxygen consumption and ammonia excretion of N. japonica were determined. Threedifferent groups in body weight (large: 2.34±0.36 g, middle: 1.50±0.21 g and small: 0.62±0.12 g) were set for all experiments. Results show that the body weight is negatively related to the rates of oxygen consumption and ammonia excretion; and the relationship is significant. The oxygen consumption and ammonia excretion at 24℃ decreased at salinity from 5 to 30 and increased above 30, indicating that both lower and higher salinity are adverse and certain degree of salinity stress is necessary for enhancing the energy demand. At salinity 30, rising temperature from 18℃ to 30℃, the oxygen consumption increased before 27℃ and then decreased. However, the relation of ammonia excretion and temperature seems more complex. Two-way ANOVA shows that salinity, temperature and body weight all have a significant effect on the oxygen consumption and ammonia excretion of the worm. Moreover, interaction between salinity/temperature and body weight is also significant. O:N (oxygen/nitrogen) ratio varies greatly in this case from 5.97 to 463.22, indicating that N. japonica can regulate the type of metabolic substrate against environment changes.     

The effect of temperature on growth and energy budget of the polychaete, Neanthes japonica Izuka

Growth and energy budget of the polychaete, Neanthes japonica, at various temperatures (17, 20, 23, 26 and 29°) were investigated in this study. The growth, as indicated by final dry weight and specific growth rate (SGR), increased with increasing temperature, with the maximum level at 26°C, and then decreased significantly at 29°C. A similar trend was observed in feeding rate, food conversion efficiency (FCE) and apparent digestive rate (ADR). However, no significant differences were detected in ADR among all the temperature treatments. In the pattern of energy allocation, faeces energy was only a small component of energy budget and had little influence on the proportion of food energy allocated to growth. The metabolic energy accounted for a large portion of energy intake for each temperature treatment. The nitrogen excretion was appreciable with changing temperature. The two expenditure terms (respiration energy and excretion energy) in energy budget were the major factors influencing the proportion of food energy allocated to growth. These results revealed that temperature affected the growth of N. japonica mainly by influencing feeding rate and FCE. In addition, regression equations describing the relationship between feeding rate, faecal production, SGR, FCE and temperature were obtained. The optimum temperatures for feeding rate, FCE and SGR were estimated at 25.01°C, 24.24°C and 24.73°C, respectively, from the regression equations.     

Population dynamics of marine ciliate Euplotes vannus (Protozoa, Ciliophora) in different artificial seawaters

To study population dynamics of marine ciliates in different artificial seawaters (ASW), the population growth dynamics of a common marine ciliate Euplotes vannus were investigated using beef extract media and rice media for five types of ASW and natural seawater (NSW). The results show that: (1) the population growth rate was in the order of NSW>Flack ASW>Nakamula ASW>Schmadz ASW>Oshima ASW>Subow ASW and was considerably higher in rice media than in beef extract media (apart from Subow ASW); (2) the maximum density of E. vannus in stationary phase in each treatment was ranked as Flack ASW>Nakamula ASW>Schmadz ASW>NSW>Oshima ASW>Subow ASW, and was again higher in rice media than in beef extract media (except for Subow ASW); (3) the exponential and stationary phases were longer in rice media than in beef extract media; (4) strains of E. vannus that had been domesticated for >1 year in ASW grew significantly slower, with lower maximum density and longer stationary phase than those isolated and maintained in NSW. It was demonstrated that: (1) E. vannus may grow well in Flack, Nakamula and Schmads ASW compared with NSW (mainly in terms of growth rate); and (2) Oshima ASW is the preferred choice for stock cultures of E. vannus, but the ASWs Flack, Nakamula and Schmadz are preferred for mass culture. These findings suggest that these three ASWs are effective for the cultivation of marine protozoa for experimental studies on ecology, toxicology and molecular biology.     

Population growth in Bejing: trends and countermeasures

Beijing’ population has experienced a dramatic increase eversince the founding of the People’s Republic of China. Population growthin Beijing can be broadly broken down into three major components:natural increase, immigration, and the growth of floating population. Thecontinuous growth of Beijing’s population is closely linked with its centralized multi-function. The comprehensive countermeasures to control Beijing’s population growth are proposed, for example, decentralizing economicfunctions, including developing the suburbs, developing the metropolitan areaand creating counter-magnetic centers, reforming the administrative and economic systems.     

Genetic improvement on Chinese shrimp （Fenneropenaeus chinensis）： growth and viability performance in F1 hybrids of different populations

Fenneropenaeus chinensis distributed in the Yellow Sea and Bohai Sea of China and the west coast of the Korean Peninsula. Different geographical populations represent potentially different genetic resources. To learn further the characteristics of different geographical population, crosses among two wild and three farmed populations were produced. The two wild populations were from the Yellow Sea and Bohai Sea (WYP), and the west coast of the Korean Peninsula and coast (WKN). The three farmed populations included the offspring of first generation of wild shrimp from coast in Korea (FKN), the Huang Hai (the Yellow Sea in Chinese) No. 1 (HH 1), and JK98. The phenotypes growth and survival rates of these populations were compared to confirm the feasibility for crossbreeding. The body length (BL), carapace length (CL), carapace width (CW), height of the second and third abdominal segment (HST), width of the second and third abdominal segment (WST), length of the first abdominal segment (LF), length of the last abdominal segment (LL), live body weight (BW), and survival rate were measured. Different combinations were statistically performed with ANOVA and Duncan's Multiple significant difference (P<0.05) in BL, CL, HST, LL, and BW; and insignificant difference (P0.05) in other growth traits and survival rate. The results of Duncan's Multiple Range Test are that BL and CL of was the best combination in all growth traits. Therefore, hybridization can introduce the variation to base populations. The systematic selection program based on additive genetic performance may be more effective than crossbreeding.     

DISTRIBUTION AND POPULATION DYNAMICS OF PARACALANUS PARVUS,PRACALANUS CRASSIROSTRIS,AND ACARTIA BIFILOSA（COPEPODA,CALANOIDA）IN THE BOHAI SEA

Paracalanus parvus,Paracalanus crassirostris,and Acartia bifilosa are dominant and widely distributed in the Bohai Sea,and comprise an important part of zooplankton in terms of biomass as well as production rate.In order to understand their seasonal distribution and population dynamics,their stage-specific abundance in different months of the year were analyzed based on the never analyzed yet samples collected in 1959.The three species showed clear and remarkable seasonal variation in abundance,which maximized in spring and summer,when they formed high biomass patches or concentrations in the nearshore area.For Paracalanus parvus,two peaks were observed in the annual circle,one in June and the other in September,For Paracalanus crassirostris,one peak occurred in summer and a small one in December.The seasonal pattern of Acartia bifilosa was different in different regions.In Bohai Bay it had a two-peak pattern,with the first large peak occurring in May and the second one in October.In Laizhou Bay,a winter peak in December and January could be observed besides the spring one.The number of generations during the reproductive season for the three species was estimated based on the annual cycle in abundance and ambient temperature.     

Microsatellite variation between Japanese eel (Anguilla japonica) and European eel (Anguilla anguilla)

1 INTRODUCTIONFreshwater eels of genus Anguilla Shaw (fam-ily Anguillidae) have complicated evolutional his-tory and are widely distributed in the world. As acatadromous fish, it spawns in the sea and grow upin rivers. Continental distribution of freshwater eelsshows that they may be related to subtropical circu-lations in the oceans, with most species inhabitingwestern Pacific Ocean, northern Atlantic Ocean andIndian Ocean (Ege, 1939; Tesch, 1977).A. japonica is an important aquacult…     

Application of restriction site amplified polymorphism (RSAP) to genetic diversity in Saccharina japonica

Restriction site amplified polymorphism (RSAP) was used, for the first time, to analyze the genetic structure and diversity of four, mainly cultivated, varieties of the brown alga, Saccharina japonica. Eighty-eight samples from varieties "Rongfu", "Fujian", "Ailunwan" and "Shengchanzhong" were used for the genetic analyses. One hundred and ninety-eight bands were obtained using eight combinations of primers. One hundred and ninety-one (96.46%) were polymorphic bands. Nei’s genetic diversity was 0.360, and the coefficient of genetic differentiation was 0.357. No inbreeding-type recession was found in the four brown alga varieties and the results of the "Ailunwan" variety using samples from 2 years showed that the variety was becoming less diverse during the selection inherent in the breeding program. Genetic diversity and cluster analyses results were consistent with these genetic relationships. The results show the RSAP method is suitable for genetic analysis. Continuous inbreeding and selection could reduce the genetic diversity effectively; therefore periodical supervision is required.     

京津冀都市圈人口集疏过程与空间格局分析

人口集疏过程及其空间格局变化是人口空间分布最直观的表现。以1982-2010年4期人口普查数据为基础,采用人口总量和人口密度指标,结合人口增减变化分级、人口商度等方法,定量分析了京津冀都市圈人口集疏过程及其空间格局变化。研究表明:近30年来,京津冀都市圈人口总量呈现持续增长趋势,人口空间分布日益不均衡,区域人口分布存在明显的南北、东西差异;人口增加是主要特征,人口集聚效应凸显,人口增加地区的县域单元比例在80%以上,以人口显著增加为主;人口减少只是零星分布,人口减少地区的县域单元比例占不到20%;人口流动比较频繁,以人口流入为主,主要流向北京、天津和河北的市辖区,人口流出地区仅是散落分布在张家口和承德的山区贫困县域;无论从静态人口指标还是动态人口分析方法,都表明京津冀都市圈人口地域集疏特征十分明显,已形成以北京、天津、石家庄为中心,其他地市(县域)人口分别向外依次扩展的人口多中心分布的圈层结构。     

耕地粮食生产潜力及人口承载力研究——以长清县为例

以长清县为例 ,利用 GIS技术和遥感数据 ,按照“机制法”原理计算耕地资源粮食生产潜力。重点是因地制宜、科学确定并计算土壤有效系数和社会有效系数 ,从而得到耕地生产潜力和社会生产潜力像元分布数据。在此基础上 ,进行粮食增产潜力计算 ,并预测未来人口数量发展趋势 ,探讨未来的人口承载力问题。结果表明 :该县粮食增产潜力巨大 ,但是粮食问题仍不容乐观。     

Genetic improvement on Chinese shrimp （Fenneropenaeus chinensis）： growth and viability performance in F1 hybrids of different populations

Fenneropenaeus chinensis distributed in the Yellow Sea and Bohai Sea of China and the west coast of the Korean Peninsula. Different geographical populations represent potentially different genetic resources. To learn further the characteristics of different geographical population, crosses among two wild and three farmed populations were produced. The two wild populations were from the Yellow Sea and Bohai Sea （WYP）, and the west coast of the Korean Peninsula and coast （WKN）. The three farmed populations included the offspring of first generation of wild shrimp from coast in Korea （FKN）, the Huang Hai （the Yellow Sea in Chinese） No.1 （HH1）, and JK98. The phenotypes growth and survival rates of these populations were compared to confirm the feasibility for crossbreeding. The body length （BL）, carapace length （CL）. carapace width （CW）, height of the second and third abdominal segment （HST）, width of the second and third abdominal segment （WST）, length of the first abdominal segment （LF）, length of the last abdominal segment （LL）, live body weight （BW）, and survival rate were measured, Different combinations were statistically performed with ANOVA and Duncan＇s Multiple Range Test. The results show that the survival rate of JK98（♀）×WKN（♂） was the highest, followed by WYP（♀）×WKN（♂）, FKN（♀）×WYP（♂）, FKN（♀）×HHI（♂） and WYP（♀）×FKN（♂）; the body weight of FKN（♀）×HHI（♂） was the highest, followed by FKN（♀）×WYP（♂）, WYP（♀）×WKN（♂）, WYP（♀）×FKN（♂） and JK98（♀）×WKN（♂）; the total length had the same ranking as the body weight. All growth traits in hybrids JK98（♀）×WKN（♂） were the lowest among all combinations. F1 hybrids had significant difference （P〈0.05） in BL, CL, HST, LL, and BW; and insignificant difference （P〉0.05） in other growth traits and survival rate. The results of Duncan＇s Multiple Range Test are that BL and CL of J     

Characterization of ribulose-1, 5-bisphosphate carboxylase/oxygenase and transcriptional analysis of its related genes in Saccharina japonica (Laminariales,Phaeophyta)

Saccharina japonica is a common macroalga in sublittoral communities of cold seawater environments,and consequently may have highly effi cient ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco)activity for carbon assimilation.In our study,we cloned the full-length Rubisco gene from S.japonica(SJ-rbc).It contained an open reading frame for a large subunit gene(SJ-rbcL)of 1 467 bp,a small subunit gene(SJ-rbcS)of 420 bp,and a SJ-rbcL /S intergenic spacer of 269 bp.The deduced peptides of SJ-rbcL and SJ-rbcS were 488 and 139 amino acids with theoretical molecular weights and isoelectric points of 53.97 kDa,5.81 and 15.84 kDa,4.71,respectively.After induction with 1 mmol/L isopropyl-β-Dthiogalactopyranoside for 5 h and purifi cation by Ni 2+ affi nity chromatography,electrophoresis and western blot detection demonstrated successful expression of the 55 kDa SJ-rbcL protein.Real-time quantitative PCR showed that the mRNA levels of SJ-rbcL in gametophytes increased when transferred into normal growth conditions and exhibited diurnal variations: increased expression during the day but suppressed expression at night.This observation implied that Rubisco played a role in normal gametophytic growth and development.In juvenile sporophytes,mRNA levels of SJ-rbcL,carbonic anhydrase,Calvin-BensonBassham cycle-related enzyme,and chloroplast light-harvesting protein were remarkably increased under continuous light irradiance.Similarly,expression of these genes was up-regulated under blue light irradiance at 350 μmol/(m 2·s).Our results indicate that long-term white light and short-term blue light irradiance enhances juvenile sporophytic growth by synergistic effects of various photosynthetic elements.     

Life cycle and production of chironomidae (diptera) in Biandantang, a typical macrophytic lake (Hubei, China)

ＩＮＴＲＯＤＵＣＴＩＯＮＡｌｔｈｏｕｇｈｄｅｓｃｒｉｐｔｉｏｎｏｆｌｉｆｅｈｉｓｔｏｒｙａｎｄｅｓｔｉｍａｔｅｓｏｆａｎｉｍａｌｐｒｏｄｕｃｔｉｏｎｈａｖｅａｌｏｎｇｈｉｓｔｏｒｙ (Ｌｉｎ ｄｅｇａａｒｄ ,1 989) ,ｆｅｗｓｔｕｄｉｅｓｏｎｔｈｅｐｒｏｄｕｃｔｉｏｎｒａｔｅｏｆｂｅｎｔｈｉｃｍａｃｒｏｉｎｖｅｒｔｅｂｒａｔｅｓｗｅｒｅｃａｒｒｉｅｄｏｕｔｉｎＣｈｉｎｅｓｅｗａｔｅｒｓ (Ｌｉａｎｇ ,1 984 ) .Ｗｉｔｈｔｈｅｄｅｖｅｌｏｐｍｅｎｔｏｆｆｉｓｈｅｒｉｅｓａｎｄｔｈｅｄｅｔｅｒｉｏｒａｔｉｏ…     

Population genetics of Sargassum horneri (Fucales, Phaeophyta) in China revealed by ISSR and SRAP markers

Sargassum horneri is a common brown macro-alga that is found in the inter-tidal ecosystems of China. To investigate the current status of seaweed resources and provide basic data for its sustainable development, ISSR (inter simple sequence repeat) and SRAP (sequence related amplified polymorphism) markers were used to analyze the population genetics among nine natural populations of S. horneri. The nine studied populations were distributed over 2 000 km from northeast to south China. The percentage of polymorphic loci P% (ISSR, 99.44%; SRAP, 100.00%), Nei’s genetic diversity H (ISSR, 0.107-0.199; SRAP, 0.100-0.153), and Shannon’s information index I (ISSR, 0.157-0.291; SRAP, 0.148-0.219) indicated a fair amount of genetic variability among the nine populations. Moreover, the high degree of gene differentiation G st (ISSR, 0.654; SRAP, 0.718) and low gene flow N m (ISSR, 0.265; SRAP, 0.196) implied that there was significant among-population differentiation, possibly as a result of habitat fragmentation. The matrices of genetic distances and fixation indices (F st ) among the populations correlated well with their geographical distribution (Mantel test R=0.541 5, 0.541 8; P=0.005 0, 0.002 0 and R=0.728 6, 0.641 2; P=0.001 0, 0.001 0, respectively); the Rongcheng population in the Shandong peninsula was the only exception. Overall, the genetic differentiation agreed with the geographic isolation. The fair amount of genetic diversity that was revealed in the S. horneri populations in China indicated that the seaweed resources had not been seriously affected by external factors.     

山东半岛城市群城市土地扩张与人口增长协调性研究

新型城镇化是以人为核心的城镇化,山东半岛城市群作为我国11个国家级城市群之一,近年来城镇化发展速度不断加快。以山东半岛城市群8个设区城市为研究对象,通过构建城市土地扩张与人口增长协调关系模型,计算8个城市的土地扩张与人口增长协调度,并根据城市土地扩张与人口增长的协调性分级标准,把1994年和2013年8个城市的土地扩张与人口增长协调性关系进行分等定级评价。结果表明:山东半岛城市群城市土地扩张与人口增长的协调性较弱,呈现出土地快速扩张态势。8个城市中有5个城市表现为土地快速扩张,其中2个城市表现为土地显著扩张,3个城市表现为土地明显扩张;人地基本协调的仅有3个,协调性空间分异明显。针对8个城市土地扩张与人口增长间不同类型的协调度,提出发展建议,促进山东半岛城市群城市土地扩张与人口增长协调发展,提高城镇化发展质量。     

ABUNDANCE AND PRODUCTION OF LIMNODRILUS HOFFMEISTERI （OLIGOCHAETA ： TUBIFICIDAE） IN ALGAE-DOMINATED LAKE HOUHU （WUHAN, CHINA）

April, 1996 to March, 1997 studies on the abundance and secondary production of Limnodrilus hoffmeisteri in an algae-dominated lake showed that the worm density and biomass peaked in December at 152 ind/m^2 and 0.087 g/m^2 , respectively. The secondary production of the animal was 0.294g/m^2 fresh wt/a. The turnover rate (P/B ratio) was 11.4.     

Abundance and production ofBranchiura sowerbyi (Oligochaeta: Tubificidae) in two typical shallow lakes (Hubei, China)

An April 1996 to March 1997 comparative study on the abundance and secondary production ofBranchiura sowerbyi Beddard, 1892 in two typical shallow lakes showed that in Houhu, an algae-dominated lake, the worm density (68 ind·m⁻²) peaked in July, biomass (1.930 g·m⁻²) peaked in June, while in Biandantang, a macrophyte-dominated lake, standing stock (density: 60 ind·m⁻²; biomass: 1.019 g·m⁻² in wet weight) peaked in December. Secondary production of the animal in Houhu Lake was 3.413 g wet wt m⁻² a⁻¹, a little more than that (2.675 g wet wt m⁻² a⁻¹) in Biandantang Lake. Their turnover rates (P/B ratios) were 4.0 and 5.0, respectively. Project (39600019 and 39430101) supported by NSFC.     

Population genetic structure of the mantis shrimp Oratosquilla oratoria(Crustacea: Squillidae) in the Yellow Sea and East China Sea

The mantis shrimp O ratosquilla oratoria is an ecologically and economically important species in the Western Pacific. In present study, the population genetic structure of O ratosquilla oratoria from the Yellow Sea and East China Sea was examined with mitochondrial DNA control region sequences. In total, 394 samples were collected from 18 locations and 102 haplotypes were obtained. For the Yellow Sea, the overall nucleotide diversity and haplotype diversity were 0.006 9 and 0.946 8, respectively; while across all the East China Sea locations, the overall nucleotide diversity and haplotype diversity were 0.027 94 and 0.979 0, respectively. The results of AMOVA and pairwise F_(ST)(0.145 2, P 0.001) revealed moderate differentiation between the Yellow Sea and East China Sea populations of O. oratoria. However, neither the neighbor-joining tree nor haplotype network showed clades with geographic pattern, which indicated considerable gene flow was existed between the Yellow Sea and East China Sea, and supporting the high larval dispersal ability in this species. Mismatch distribution analysis and neutrality tests suggested that O. oratoria has undergone population expansion event, and the Pleistocene glacial cycles might have an impact on the historical demography of O. oratoria. The genetic information obtained in this study can provide useful information for sustainable improvements for capture fisheries management strategies.     

Estimation of natural mortality coefficient from fish abundance and catch data using Virtual Population Analysis (VPA)

1 Introduction Natural mortality coefficient (M) is one of the key population parameters common to most analyses in fish stock assessment. Many mathematical models of fish stock dynamics include M directly or indirectly. However, until now M is the least known among the four factors affecting fish stock variation: recruitment, growth, natural death and fishing death (Quinn and Deriso, 1999). There is an extensive literature concerning the impact of errors in M on stock assessment (Mertz and…