Factors influencing seed germination of Cyperus capitatus, inhabiting the moving sand dunes in southern Europe

Cyperus capitatus Vandelli (Cyperaceae) is distributed in coastal sandy habitats and mobile dunes of south Europe. Its seed germination ecology is not known, despite its potential to be used in re-vegetation projects. Laboratory experiments were conduced to assess the effects of salinity, light regime, cold stratification and burial on seed germination of this species. Overall, increasing salinity delayed germination, increased seed dormancy and mean time to germination (MTG), and reduced final germination percentage, inhibiting it completely above 1% of salinity; although it did not affect seed viability. C. capitatus seeds exhibited their greatest germination at levels between 0 and 1% in non-stratified seeds, and between 0 and 0.5% for stratified seeds. Thus, the effect of salt was greater for stratified seeds at 5 °C. Germination in light/darkness conditions was similar to that in full darkness. Finally, burial in sand of C. capitatus seeds appeared to have a significant effect on cumulative percentage of germination. Seeds buried at depths greater than 2 and 3 cm showed a lower germination success than those on sand surface or buried at shallower depths. Burial also affected the beginning and speed of seed germination.     

Dispersal and germination ofProsopis flexuosa(Fabaceae) seeds by desert mammals in Argentina

The ‘algarrobo’Prosopis flexuosais an important food resource in the Monte Desert of Argentina. Native, domestic, and exotic mammals consume the fruit of this legume and disperse the seed through faeces. In the present study we analyse the effect that different dispersal agents (cattle, horse, European wild boar, rodents, gray fox) have on seed damage, viability, and germination. Cattle increase germination at the expense of reduced viability, whereas horses maintain viability but do not contribute to a prompt germination response. Among native mammals, the gray fox maintains seed viability without increasing germination, whereas rodents affect seed viability but enhance germination rates. The European wild boar, however, damages all of the seeds it consumes.     

Effects of salt and alkali stress on Reaumuria soongorica germination

Seed germination and early seedling growth are crucial stages for plant establishment. Two neutral (NaCl and Na₂SO₄) and two alkali (NaHCO₃ and Na₂CO₃) salts were selected to investigate their effects on germination and recovery responses in Reaumuria soongorica. Results show that both salt types significantly reduced germination and radicle elongation. The rate of germination and emergence of R. soongorica seeds continuously decreased as salinity increased, and the time to achieve maximum germination rate was delayed. The speed of seed germination dropped rapidly as salt concentration increased. Alkaline salts restricted the germination rate of R. soongorica seeds, and stresses resulting from alkaline salts and high concentrations of neutral salts resulted in many deformed seedlings. The length of the radicle and germ decreased with increasing salt concentration, but certain concentrations of salt and increased pH promoted germ growth. The results of regression analysis show that salt concentration was the dominant factor inhibiting R. soongorica seed germination rate. Salinity, buffering capacity, and pH all affected embryo growth, but salinity had the most pronounced effect. Seed viability under highly saline conditions appears to be a better indicator of adaptation to saline environments than seed germination under saline conditions.     

Seed predation and moisture limit germination of recalcitrant Dobera glabra (Salvadoraceae) seeds in the Afar rangelands, Ethiopia

We studied current recruitment failure of Dobera glabra, an important famine-food throughout the dryland parts of the Horn of Africa. The species has previously been found to have recalcitrant seeds, a strategy not common for plants in dryland areas. We experimentally tested (1) how germination capacity of D. glabra is affected by seed form, storage period and moisture under nursery conditions, and (2) how seed predation by vertebrate herbivore and seed exposure at germination site affect germination under field conditions in northern Afar rangelands, Ethiopia. D. glabra seeds that received either mulching or supplementary watering (3 days/week) had a higher germination success than controls. The seeds are probably desiccation sensitive since stored seeds had poor germination performance compared to fresh seeds. Seeds in the rangeland plots protected from herbivores had significantly higher germination success compared to open plots. Our findings demonstrate that seed predation and moisture limitation highly reduced the germinability of the recalcitrant D. glabra seeds, indicating that the species might have persisted due to past wetter periods and low herbivore density in dry environments.     

Critical conditions for successful regeneration of an endangered annual plant,Cryptantha minima: A modeling approach

Tiny cryptanthe (Cryptantha minima Rydb.) is an endangered annual plant adapted to prairie sand dunes of Canada. Thermal time and hydrothermal time models were used to quantify the effect of temperature and water potential on seed germination and to relate it to field conditions. Tiny cryptanthe seeds had a base temperature of ?3.9 °C for germination. Final germination percentage was highest at temperatures near the freezing point. Seeds required approximately 130.6 °C d to reach 50% germination under optimal water conditions. Seed germination was sensitive to water stress and large seeds had lower germination than small ones especially at reduced water potentials and higher temperatures. The high potential germination and low seed dormancy indicate that tiny cryptanthe lacks a dormancy mechanism, but the unique germination response to temperature enables it to fully utilize water from spring snowmelt before drought in the summer. Future climate change with higher temperatures could reduce regeneration success of this species unless there is an associated increase in winter and spring precipitation.     

Pre- and post-dispersal seed loss and soil seed dynamics of the dominant Bulnesia retama (Zygophyllaceae) shrub in a sandy Monte desert of western Argentina

In deserts and semi-deserts dominant plants seldom rely on a persistent seed bank to ensure their recruitment from sexual reproduction, which is generally based on the seeds produced in the last reproductive event. We studied pre- and post-dispersal seed loss of Bulnesia retama, a dominant shrub of the sandy Monte deserts (western Argentina). We hypothesised that pre- and post-dispersal seed depletion in B. retama regulate soil seed density and in turn decrease soil seed reserves. We also hypothesised that spatial patches induced by standing vegetation and other microsites additionally affect post-dispersal seed loss and soil seed density.We observed that as much as half the total seed production was lost due to pre-dispersal seed predation. Seed dispersal plus predation showed a negative exponential function with 61% of seeds falling during the first 28 days, and 99% after 90 days, when 34% of the seeds remained inside excluded branches. A significant patch effect was observed on soil seed density and seed dynamics, with a greater number of seeds and increased seed depletion rates below B. retama shrubs. However, spatial patches did not affect seed germination although they increased with burial time, from 22% to 70% after 75 and 276 days, respectively. Likewise, a similar trend was found in potential seed germination; seeds collected directly from mother plants had a low germination rate (less than 25%), which increased to 75% after 276 days of soil burial. About 15% of the dispersed seeds should persist to the next rainy season, thus contributing to a low-density but persistent seed bank.The delay in seed germination and temporal decrease in soil seed density that we observed for B. retama suggest that a set of mechanisms have been developed promoting germination events whenever soil water is available, at any moment of the year.     

Effect of sand burial depth on seed germination and seedling emergence of Calligonum L. species

A greenhouse study was conducted to determine the effects of sand burial on seed germination and seedling emergence of ten Calligonum L. species. The results suggested that the deeper the seeds in sand, the lower and slower their germination and seedling emergence. Seedling emergence occurred from a maximum depth of 12 cm with most seedlings emerging from 2 and 4 cm depths. The percent seedling emergence, number of days of first emergence, percent seed germination and percent of dormant seeds were significantly affected by different species and burial depth. There was a significant positive correlation between the number of days to first emergence and burial depth. Both mean percent germination and percent seedling emergence were negatively correlated with depth significantly for each species.     

Effect of burial on the germination of Opuntia tomentosa's (Cactaceae,Opuntioideae) seeds

Opuntia tomentosa seeds have physiological dormancy and a permeable but hard funicular envelope that restricts water uptake and embryo growth. Dormancy break, germination, and changes in the funiculus were compared in laboratory-stored seeds and in those buried for 7 months at different microsites and later exhumed and stored in the laboratory. The funicular envelopes of both lots were examined with a scanning electron microscope (SEM). Seeds in both lots were: scarified with H₂SO₄ (0, 45, 60 and 90 min), treated with gibberellins (0, 1000 and 2000 ppm) and germinated at the same ages (0, 2, 4, 7, and 10 months after exhumation) at 24 °C. Light effect was determined at 24 °C and 20–35 °C. In laboratory-stored seeds germination was low. Exhumed seeds germinated in ～50%, the remaining ones required scarification and gibberellins, but lost dormancy two months later. Gibberellins reduced germination heterogeneity, and scarification reduced germination; the adverse effect of scarification was offset by gibberellins. Exhumed seeds germinated mainly in darkness at 20–35 °C and partially in far red light at both temperatures. SEM showed that fungi eroded the funiculus reducing its resistance. O. tomentosa could form a seed bank, ensuring its survival under heterogeneous environments.     

沙埋对六种沙生植物种子萌发和幼苗生长的影响

分别对6种植物的种子进行0 cm、0.5 cm、1 cm、2 cm、3 cm、4 cm、6 cm、8 cm、12 cm 九个深度的沙埋处理。结果表明：①对于所有的植物种子,0 cm沙埋较浅层沙埋有较低的出苗率;②沙埋对所有植物种子的幼苗出土均有显著的影响(P<0.05),随着埋沙深度的增加,植物幼苗的出土均显著的下降;③植物种子幼苗出土的最大深度与繁殖体的大小呈现显著的正相关性,繁殖体大的植物种能从更深的沙层中出苗。     

Effect of environmental heterogeneity on field germination of Opuntia tomentosa (Cactaceae,Opuntioideae) seeds

We studied in Opuntia tomentosa the effect of plant shade (orientation and distance), and the place where the seeds remained before germination. Seeds were collected in 1998 and a) sown immediately on soil (non-buried seeds), b) stored in the laboratory and sown on soil during the 1999 rainy season (control) or c) buried after collection (under the shade of a tree, a rock and in an open space), exhumed 7 months later and sown in 1999 (exhumed seeds). Seeds were sown under or outside the shade of several trees; or on the northern, eastern and western sides of a 14-m-tall tree. In 1999, germination was high in all samples, but the control seeds had delayed germination. After two rainy seasons, non-buried seeds had the lowest germination rate. Germination was not affected by the distance from the tree. Outside of the plant's shade, germination was low. Germination was affected more by the environmental heterogeneity than by the individual plant shade. Germination probabilities were the highest at 16–19 °C; field germination was zero at 20.6 °C. Seed polymorphism and microenvironmental heterogeneity spread germination across two rainy seasons creating a permanent seed bank.     

Soil seed bank longevity of the exotic annual grass Bromus rubens in the Mojave Desert,USA

Although arid environments are often considered among the least invaded terrestrial biomes, the impacts of exotic plant species can be severe and long lasting. Bromus rubens (red brome) is an exotic annual grass species in the Mojave Desert known to outcompete native plant species, alter habitat, and promote accumulation of fuel that contributes to increasing fire frequency and severity. We assessed longevity of the exotic B. rubens seeds in the soil by burying seeds at four depths (0, 2, 5, and 10 cm) and recovering seeds 6, 12, 18, and 24 months after burial. Seed viability was reduced with greater burial depth and greater time since burial. A relatively small proportion of seeds retained viability for two years, suggesting that while the B. rubens seed bank can be large, it is relatively short-lived. Although B. rubens apparently relies more on the annual production, dispersal, and germination of seeds than on a long-lived seed bank for its annual recruitment, the numerous seeds produced by individual plants indicate that even a small proportion of seeds remaining viable for more than a year can aid recruitment from the seed bank and is an important factor in understanding population dynamics.     

Factors controlling regeneration in a rare desert tree Acacia peuce: Limits to soil seed bank accumulation in time and space

We assessed reasons for rare recruitment in Acacia peuce, an Australian arid zone tree, by testing recruitment-limitation hypotheses relating to seed bank inputs and losses. Viable seed occurred in low density in the soil (<1 seed m²) whereas freshly produced seed was non-dormant and germination was high and fast, indicating small rain events can deplete seed banks without providing sufficient moisture for seedling survival. Seed bank seed had lower viability than fresh seed. Insect predation of seed was negligible but seed pod production and seed number/pod were low. Thus, seed bank accumulation is limited by low inputs and continual high seed losses through germination and potentially seed death. No seed shadow effect under adult canopies was found, but dispersal beyond existing stands was negligible. Seed dispersal from parent plants occurred by wind movement of unopened pods whereas the resident ant fauna did not transport seed. Microsite parameters strongly influenced horizontal distribution of buried seed, with all seed being retrieved from vegetated sandy mounds. Overall, we found that seedling recruitment is likely to be confined to large above average rainfall events because the species is unlikely to maintain a seed bank in below-average and average rainfall phases.     

甜瓜种子萌发和幼苗生长对NaCl胁迫的响应

在盆栽条件下研究了NaCl胁迫对黄河蜜和白沙蜜甜瓜种子萌发和幼苗生长的影响。结果表明:NaCl胁迫对甜瓜种子吸水和累计发芽率影响较小,但明显延缓后期吸水和发芽进程。50 mmol·L-1以上NaCl浓度下,甜瓜种子相对发芽指数、相对活力指数和胚根生长量均下降。NaCl胁迫抑制甜瓜幼苗高度、株叶面积增长和干物质积累,抑制作用具有浓度和时间依赖性。NaCl胁迫对甜瓜幼苗形态生长的抑制作用强于对物质积累的抑制作用,对根系干物质积累的抑制作用高于对地上部干物质积累的抑制作用,对白沙蜜的抑制作用强于对黄河蜜的抑制作用。甜瓜种子和幼苗能适应的临界NaCl浓度在100~150 mmol·L-1之间,厚皮甜瓜可能更适合作为咸水灌溉和盐碱地生产的经济作物。     

Passage time, viability, and germination of seeds ingested by foxes

Seed passage through the gut of vertebrates is critical for endozoochorus seed dispersal because it may influence seed viability and germination capacity. Foxes are important fruit-eaters and presumed seed dispersers in the Chaco ecoregion. We experimentally assessed the seed passage time (SPT) and its effect on seed viability and germination in two fox species (Pseudalopex gymnocercus and Cerdocyon thous) that occur in the Chaco. Passage time was measured using both artificial seeds of three sizes and wild seeds of four native fleshy-fruit species commonly consumed by foxes (Ziziphus mistol, Acacia aroma, Celtis tala, and Syagrus romanzoffiana). The average SPT was 6.8 h for the Pampa fox and 7.7 h for the crab-eating fox. Passage time did not differ either among seeds of different sizes or between fox species. Passage through digestive tract of both fox species did not affect seed survival, whereas germination rate was slightly improved. Our data suggest that both fox species are legitimate seed dispersers of the four species studied, having a positive effect on both germination rate and dispersal distance from the parent plant.     

水分和沙埋对4种沙生植物种子萌发和出苗的影响

通过室内试验研究了土壤水分和沙埋深度对4种沙生植物种子萌发和出苗的影响。结果表明:草本雾冰藜（Bassia dasyphylla）种子萌发和幼苗出土喜好水分,水分含量越高,种子发芽率越高;半灌木黑沙蒿（Artemisia ordosica）、灌木沙冬青（Ammopiptanthus mongolicus）和沙棘（Hippophae rhamnoides）种子在含水量为15%~20%的土壤中发芽率高,干旱或水分含量过高会导致种子发芽率降低。沙埋深度显著影响种子的萌发和出苗,雾冰藜种子在无沙埋时发芽率最高,黑沙蒿适宜沙埋深度为0.5 cm,沙冬青和沙棘适宜沙埋深度1.0 cm。     

Disentangling Fabiana imbricata (Solanaceae) regeneration: The importance of disturbance and rainfall

In Mediterranean ecosystems, fire influences plant population dynamics and changes plant community structure by affecting germination and establishment of seeder shrubs. Fabiana imbricata is a long-lived seeder shrub with a broad distribution in South America. In Northwestern Patagonia grasslands there are many records of F. imbricata recruitment after fires. We hypothesized that recruitment is promoted by: 1) soil erosion that exposes deeply buried seeds; 2) direct fire effects such as heat that could break seed dormancy; 3) indirect fire effects (i.e. increase of light availability and elimination of allelopathic substances) and 4) water availability in spring. In field conditions, we tested the effects of shading and soil disturbance on F. imbricata recruitment and seed availability and distribution in the soil profile. Under controlled conditions, we investigated the influence of leaf leachate, light, heat and water on seed germination and seedling emergence. Seed germination was inhibited by leachate, and seedling emergence was inhibited by seed heated to 80 °C and 120° for 5 min. F. imbricata seedlings only emerged in germination treatments that simulated wet and very wet spring conditions. Fire and postfire wind that favor seed exposure, combined with high precipitation early in the growing season, would be necessary for successful shrub recruitment. When F. imbricata shrubland colonizes the grassland, it strongly modifies local vegetation structure, reduces species richness and increases fuel loads. F. imbricata is a key species in the ecosystem specially related to fire regime. Information on the postfire regeneration of this species would provide valuable knowledge of the changes in biomass accumulation in a fire-prone Mediterranean ecosystem.     

The effect of biological soil crusts of different successional stages and conditions on the germination of seeds of three desert plants

Biological soil crusts greatly affect soil surface properties, which, in turn, may alter seed germination of vascular plants. We investigated the effects of two types of biological soil crusts (moss crust and algal crust) at different ages on the seed germination of Bassia dasyphylla and Artemisia ordosica. We also investigated the effects of crust conditions (living crust and dead crust) on seed germination of B. dasyphylla, A. ordosica and Eragrostis poaeoides. The presence of both algal and moss crusts significantly enhanced the germination of B. dasyphylla and A. ordosica compared with uncrusted soil. Crust age had no significant effect on the germination of either B. dasyphylla or A. ordosica for both moss and algal crusts. Germination of B. dasyphylla differed significantly between moss and algal crusts, but germination of both A. ordosica and E. poaeoides did not. Germination of A. ordosica, E. poaeoides and B. dasyphylla was significantly higher on living algal crust than on dead crust and uncrusted soil, while living moss crust enhanced the germination of both A. ordosica and B. dasyphylla but had no significant effect on E. poaeoides.     

Seed biology of Australian arid zone species: Germination of 18 species used for rehabilitation

Revegetation of disturbed land, particularly in arid environments, is often hindered by low seedling establishment. Information on seed biology and germination cues of keystone species is lacking, particularly in arid Australia; a major zone for mining developments. This study investigated seed characteristics and germination of 18 common species required for rehabilitation of disturbed areas at Shark Bay Salt in the Shark Bay World Heritage Area, Western Australia. Untreated seeds of seven species (Aphanopetalum clematideum, Atriplex bunburyana, Austrostipa elegantissima, Melaleuca cardiophylla, Pembertonia latisquamea, Rhagodia baccata, Salsola tragus) exhibited high germination percentages. Seeds of two species (Acacia tetragonophylla, Stylobasium spathulatum) had low imbibition, which increased with hot-water treatment, hence require scarification for germination. Gibberellic acid, smoke water and karrikinolide (a butenolide isolated from smoke) substantially increased germination percentages of three species (Anthocercis littorea, Diplolaena grandiflora, Solanum orbiculatum). Seeds of the remaining six species (Dioscorea hastifolia, Eremophila oldfieldii, Nitraria billardierei, Ptilotus exaltatus, Thryptomene baeckeacea, Zygophyllum fruticulosum) had low germination percentages regardless of treatment. Most species germinated equally well at 26/13 °C and 33/18 °C, however seven species had improved performance at 26/13 °C. This study is significant to land managers and conservation agencies with an interest in optimising germination of arid zone seeds for restoration.     

Seed germination of wild,in situ-managed,and cultivated populations of columnar cacti in the Tehuacán-Cuicatlán Valley,Mexico

Seed germination was compared among wild, in situ-managed (wild plants let standing in areas cleared for agriculture), and cultivated populations of the columnar cacti Stenocereus pruinosus, Polaskia chichipe, Myrtillocactus schenckii, and Polaskia chende, species representing a gradient from higher to lower management intensity, respectively. We hypothesized that seeds from cultivated populations have higher water requirements to germinate than seeds of other populations, and that such difference is stronger in species more intensely managed. Germination was evaluated under water potential treatments at 0.0, ?0.2, and ?0.4 MPa. Interspecific differences were identified; germination rates markedly decreased in S. pruinosus and P. chichipe as water potential reduced. M. schenckii seeds germinated better at ?0.2 MPa, and seeds of P. chende in all treatments. Seed germination of wild and cultivated populations was similar in all cases and, therefore, at the population level domestication does not appear to have influenced variations in germination of the studied cacti species. However, experiments to test whether germination of seeds from plants with clear signs of domestication differs with seeds from other plants and their differential germination and seedling survival in wild, in situ-managed and cultivated environments could help to precise the influence of domestication on these plants.     

Factors controlling the regenerative cycle of Thymus funkii subsp. funkii in a semi-arid gypsum steppe: A seed bank dynamics perspective

We researched the soil seed bank dynamics of Thymus funkii subsp. funkii, a gypsovag shrub endemic to the southeastern Iberian Peninsula, to determine possible reasons for low recruitment in isolated populations growing on crusted gypsum soils. Recruitment-restriction hypotheses involving main factors in the seed bank dynamics were analysed, and a diagrammatic dynamics model drafted from a population perspective. Viable seed production averaged 362 per plant. Seed shadows resulting from primary dispersal were concentrated under the mother plant. There were two thyme-seed predators: coleopteran larvae (pre-dispersal phase) and Messor bouvieri ants (pre- and post-dispersal phases), which predated 16.4% and 3.8% of population yield, respectively. However, ants may simultaneously contribute to the spatial secondary dispersal, since they lost a small fraction of seeds they harvested. Seeds showed innate physiological dormancy to high summer temperatures, although this disappeared rapidly. The response of germination to other temperature × light conditions was wide and rapid. No permanent seed bank was detected in the soil. Seeds buried in the upper soil layer (2 cm) germinated highly and fast. In contrast, a significant fraction of seeds buried deeply (8 cm) remained viable for over one year. Germination in the field accounted for only 1.0% of the seed output, and seed death due to pathogen attack and aging was also low (3.0%). Seedling emergence was confined to autumn and showed a high correlation with total plant cover, irrespective of plant species. Seedling survival was very low. The small spatial correspondence between primary dispersal and seedling emergence patterns may reflect the importance of secondary dispersal in T. funkii seed bank dynamics. Hypotheses and consequences of such horizontal seed movements for conservation management are discussed.