H5、H7和H9亚型禽流感分子鉴别诊断方法的建立

为快速、敏感、特异地鉴别诊断现较为重要的禽流感病毒亚型,根据H5、H7和H9亚型禽流感病毒HA基因序列的保守区域设计出并合成3对特异性引物,利用这3对引物对H5、H7和H9亚型禽流感病毒的HA基因片断进行多联RT-PCR扩增,并对多联RT-PCR检测方法进行敏感性及特异性实验。结果表明:3对引物能特异性地扩增出H5、H7和H9亚型禽流感病毒HA基因片段,大小分别为490bp,365bp和686bp;该检测方法敏感性高,特异性强;初步建立起快速、敏感、特异地鉴别检测H5、H7和H9亚型禽流感病毒的分子诊断方法。     

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新疆阿克苏断层H2浓度影响因素及映震效能分析

利用相关和回归分析方法,分析断层H2浓度与气温、气压之间的相关关系,得到气温与断层H2浓度的回归方程.通过R值评分方法对断层H2浓度回归残差进行映震效能检验,结果表明:1)气温为阿克苏断层H2浓度变化的主要影响因素,与断层H2浓度呈高度正相关;气压对阿克苏断层H2浓度变化影响较小,与断层H2浓度呈中低度负相关.2)断层...     

线性状态等式约束下两种H∞滤波的比较

近年带有线性等式约束的H∞滤波在实际应用中越来越广泛,针对此种情况,从理论上研究了线性状态等式约束下两种H∞滤波状态估计性能的比较问题。在投影系统和H∞滤波方程的基础上,考虑线性状态等式约束条件,给出了一种含线性状态等式投影系统约束的H∞滤波。通过比较其与状态投影下的H∞滤波的估计性能,指出并证明了投影系统约束的H∞滤波的估计性能优于状态投影下的H∞滤波的估计性能。最后给出一个数值模拟例子,来验证结论。     

胶东玲珑矿田石英脉型金矿床中流体包裹体特征研究

玲珑矿田含有两种类型金矿床,一是蚀变岩型金矿床,二是石英脉型金矿床。该文重点研究玲珑金矿田西山矿区石英脉型金矿床中流体包裹体的特征。石英脉型金矿床分布在玲珑帚状构造之中,含金石英脉受"帚状构造"的断裂和节理控制,有300余条。流体包裹体显微观察研究表明,玲珑石英脉型金矿新鲜花岗岩和钾化花岗岩主要含有H2O包裹体,其次有少量H2O-CO2包裹体;(黄铁)绢英岩通常含较粗粒石英集合体,石英中有丰富的H2O,特别是H2O-CO2包裹体;金矿体(含金石英脉)含有明显数量的H2O-CO2包裹体;特别是存在较大的三相H2O-CO2包裹体。不同期次(世代)石英中H2O和H2O-CO2包裹体的大小、形态、相对含量和丰度变化较大。     

数值计算在地磁FHD仪器干扰排除中的应用

FHD分量质子磁力仪是一种用来测量地磁总场F、水平分量H（计算得出垂直分量Z）、相对磁偏角D的数字化分量质子磁力仪,观测中发现,作为绝对连续观测F、H、Z分量会出现漂移的现象,引起漂移的因素有记录墩的倾斜、温度的影响等。通过观测实例与理论计算得出：1）由于观测墩倾斜造成的漂移,水平分量H增大,垂直分量Z减小,地磁总场F基本不变,绝对值变化H大于Z,且影响量可通过数值模拟估算得出;2）由于场地铁磁性物质造成的漂移,水平分量H增大,垂直分量Z减小,地磁总场F减小,绝对值变化Z大于H。     

Allelopathic eff ects of macroalga Corallina pilulifera on the red-tide forming alga Heterosigma akashiwo under laboratory conditions

Over the past few years, harmful algal blooms(HABs), such as red tides, have been frequently observed in coastal zones worldwide. The natural allelopathic interactions among macroalgae and red tide microalgae can alter the structure and succession of aquatic ecosystems. We investigated the influence of four environmental factors(temperature, salinity, light, and p H) on the allelopathic eff ects of the macroalgae Corallina pilulifera on red-tide forming Heterosigma akashiwo under laboratory conditions. Each of the factors had four levels: temperature(15, 20, 25, and 30°C), salinity(10, 20, 30, and 40), light(20, 100, 200 and 400 μmol/(m~2 ?s)), and p H(5.5, 7, 8.5, and 10. Two-factor experiments were designed for each two environmental factors, with six combination treatments(temperature-salinity, temperature-light, temperature-p H, salinity-light, salinity-p H, and light-p H). Results showed that the allelopathic eff ect was significantly influenced by temperature, salinity, light, and p H. As single factors, the low temperature(15°C), low salinity(10), high-intensity light(400 μmol/(m~2 ?s)), and high p H(10) treatments substantially enhanced the allelopathic eff ect. The strongest allelopathic eff ect of C. pilulifera on H. akashiwo was observed under the following treatments: 15°C and salinity of 40, 25°C and p H 10, 25°C with medium- to high-intensity light at 200–400 μmol/(m~2 ?s), 400 μmol/(m~2 ?s) and salinity of 10, 400 μmol/(m~2 ?s) and p H 10, and p H 10 with a salinity of 40.     

Strain H<Subscript>2</Subscript>-419-4 of <Emphasis Type="Italic">Haematococcus pluvialis</Emphasis> induced by ethyl methanesulphonate and ultraviolet radiation

Two strains H2-410 and H2-419 were obtained from the chemically mutated survivors of wild Haematococcus pluvialis 2 by using ethyl methanesulphonate (EMS). Strains H2-410 and H2-419 showed a fast cell growth with 13% and 20% increase in biomass compared to wild type, respectively. Then H2-419-4, a fast cell growth and high astaxanthin accumulation strain, was obtained by exposing the strain H2-419 to ultraviolet radiation (UV) further. The total biomass, the astaxanthin content per cell, astaxanthin production of H2-419-4 showed 68%, 28%, and 120% increase compared to wild H. pluvialis 2, respectively. HPLC (High Performance Liquid Chromatography) data showed also an obvious proportional variation of different carotenoid compositions in the extracts of H2-419-4 and the wild type, although no peak of carotenoids appeared or disappeared. Therefore, the main compositions in strain H2-419-4, like its wild one, were free of astaxanthin, monoester, and diester of astaxanthin. The asexual reproduction in survivors after exposed to UV was not synchronous, and different from the normal synchronous asexual reproduction as the mother cells were motile instead of non-motile. Interestingly, some survivors from UV irradiation produced many mini-spores (or gamete?), the spores moved away from the mother cell gradually 4 or 5 days later. This is quite similar to sexual reproduction described by Elliot in 1934. However, whether this was sexual reproduction remains questionable, as no mating process has been observed.     

马氏珠母贝Su(H)基因克隆与组织特异性表达

Su(H)基因(Suppressor of Hairless gene)对生物体细胞的分化、增殖和凋亡起重要的调控作用。根据马氏珠母贝(Pinctada martensii)转录组数据库中注释为Su(H)的unigene序列设计基因特异性引物,应用cDNA末端快速扩增(RACE)技术克隆获得了马氏珠母贝Su(H)[pm-Su(H)]基因cDNA全长序列。结果表明:pm-Su(H)基因全长3 830 bp,其中开放阅读框含有1 920 bp,编码640个氨基酸残基,5'UTR为1 568bp,3'UTR为342 bp,含有27 bp polyA;预测其分子质量为70.6 ku,等电点为7.38;多序列比对显示,pm-Su(H)与其他物种的Su(H)有较高的保守性,与牡蛎(Crassostrea gigas)的同源序列高达80%;荧光定量PCR分析表明,pm-Su(H)在马氏珠母贝闭壳肌、鳃、珍珠囊、外套膜、肝胰脏、性腺、足、血淋巴等8组织中均有表达,其中以性腺中表达量最高,其次是珍珠囊和外套膜。     

Morphological study of the genus Herposiphonia(Rhodophyta,Rhodomelaceae) on the coast of eastern Guangdong,China, with a description of H. pinnata sp. nov.

We present a taxonomic study of taxa of the red algae genus Herposiphonia(Rhodophyta, Rhodomelaceae), collected from the coast of eastern Guangdong, China. We made detailed morphological studies and considered recent taxonomic criteria for species delimitation, and are making the first report of five diff erent species on the coast of Guangdong, including a new species. The species identified were H. caespitosa Tseng, H. hollenbergii Dawson, H. pecten- veneris(Harvey) Falkenberg, H. subdisticha Okamura and H. pinnata Ding and Tan sp. nov. H. pinnata sp. nov. is characterized by bright green thalli; most parts of the feathery thalli are free of the substratum; determinate branches and indeterminate branches are arranged in a chaotic sequence; the primary axis has bare segments; the determinate branch has 9–11 periaxial cells per segment; vegetative trichoblasts are abundant; and tetrasporangia are formed on the middle of the determinate branch with 1–8 successive segments in a single rectilinear series. This paper is also the first record of sporophyte plants of H. pecten-veneris.     

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天文(重力)水准的新手段及其计算方法

介绍当今确定天文大地垂偏差的新仪器－CCD自动天体测量仪和确定两点间高程异常差的新方法，该方法用单极坐标代替繁琐的双极坐标进行计算，讨论了天文重力水准的误差及垂线偏差非线性影响等问题，估计在不久的将来，用这一手段施测山区似大地水准面的精度可望达到厘米级。     

论多种数据联合反演的模式及算法

针对日益丰富的数据资料，综述了大地测量反演的发展进程，论述了多种数据联合反演的意义和必要性，给出了兼顾权比确定的大地测量、地震和地质三类数据的联合反演模式及采用水准测量、GPS和重力三种数据联合反演断层参数的具体公式，特别介绍了求解优化问题所有全局最优解的区间算法，并给出了基本算例，结果体现了区间算法无可比拟的优越性及其在大地测量反演领域中的应用前景。     

位错模式反演的算法研究

综述了大地测量反演算法的发展状况，详细介绍了模拟退火，随机耗费和区间算法等3种优化方法，并基于位错模式，采用模拟的重力测量观测数据，比较了3种算法的反演效果，结果表明：模拟退火法优于随机耗费法，而区间算法的可靠性又优于模拟退火法，区间算法是最可靠的反演方法。     

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