Spatial and temporal distribution of landlocked Galaxias maculatus and Galaxias platei (Pisces: Galaxiidae) in a lake in the South American Andes

Galaxiids are present in many of the Andean lakes in southern South America. We studied landlocked Galaxias maculatus (Jenyns) and Galaxias platei Steindachner populations in a deep oligotrophic lake (Lake Gutiérrez, Patagonia, Argentina). Their temporal and spatial distribution, intralacustrine movements (horizontal and vertical), and spawning periods were analysed using several sampling techniques (ichthyoplankton net, seine net, gill net, and baited benthic taps). We identified the early life stages of both species based on their morphology and otolith shape. The free embryos of both species migrate to the limnetic zone, where they coexist as larvae, facing the same food availability and probably the same predation risk. Each species then moves on to its own juvenile and adult habitat: the littoral and benthic zone for G. maculatus and only the deeper benthic zone for G. platei. Their adult habitats and part of their spawning periods partly overlap.     

Effect of cadmium, copper and mercury on antioxidant enzyme activities and lipid peroxidation in the gills of the hydrothermal vent mussel Bathymodiolus azoricus

Metals are known to influence lipid peroxidation and oxidative status of marine organisms. Hydrothermal vent mussels Bathymodiolus azoricus live in deep-sea environments with anomalous conditions, including high metal concentrations. Although B. azoricus are aerobic organisms they possess abundant methano and thioautotrophic symbiotic bacteria in the gills. The enzymatic defences (superoxide dismutase (SOD), catalase (CAT), total glutathione peroxidase (Total GPx) and selenium-dependent glutathione peroxidase (Se–GPx)) and lipid peroxidation were determined in the gills of B. azoricus exposed to Cd (0.9 μM), Cu (0.4 μM) and Hg (0.1 μM) with different times of exposure. The experiments were performed in pressurized containers at 9 ± 1 °C and 85 bars.Results show that vent mussels possess antioxidant enzymatic protection in the gills. Cd and Cu had an inhibitory effect in the enzymatic defence system, contrarily to Hg. These enzymatic systems are not completely understood in the B. azoricus, since reactive oxygen species might be produced through other processes than natural redox cycling, due to hydrogen sulphide and oxygen content present. Also the symbiotic bacteria may play an important contribution in the antioxidant protection of the gills.     

短蛸(Octopus ocellatus)酪氨酸蛋白激酶基因的克隆及其mRNA在细菌刺激后的表达规律

从前期构建的短蛸(Octopus ocellatus)c DNA文库中克隆获得了一个短蛸酪氨酸蛋白激酶(Oo Btk)基因的c DNA全长,其c DNA全长1191 bp,包括5’非编码区(UTR)259 bp,3’UTR 227 bp,开放阅读框(ORF)705 bp,编码234个氨基酸,预测理论等电点为8.50,分子量为27.7 k Da。运用实时荧光定量PCR法分析了在健康短蛸的不同组织以及鳗弧菌和藤黄微球菌刺激后血细胞中Oo Btk的表达规律,结果表明:Oo Btk在肌肉、外套膜、鳃、鳃心、系统心脏、肝胰脏、肾囊、胃、性腺和血细胞等各检测组织中均有表达,其中在肝胰脏的表达量最高;短蛸经鳗弧菌和藤黄微球菌刺激后,Oo Btk在血细胞中的表达量呈现出了明显的被诱导表达趋势,在鳗弧菌和藤黄微球菌刺激后6 h表达即增强,并分别在鳗弧菌刺激后48 h和藤黄微球菌刺激后12 h达到最高。Oo Btk参与短蛸对于鳗弧菌和藤黄微球菌刺激的应答过程。     

深海化能极端环境中劳盆拟刺铠虾(Munidopsis lauensis)的全长转录组测序分析

劳盆拟刺铠虾(Munidopsis lauensis)是一种十足目铠甲虾, 能够适应深海化能合成生态系统的极端环境, 但是其已知基因序列信息却很少。为此, 采用PacBio平台的SMRT测序技术对来自南海台西南冷泉劳盆拟刺铠虾的肝胰腺、鳃、肌肉和肠混合组织进行了三代全长转录组测序。通过聚类、校正、去冗余之后共获得28 811条高质量isoform, 平均长度为2 086 bp, N50长度为2 275 bp。使用Nr、SwissPort、KEGG、KOG数据库对转录本序列进行功能注释, 共有20 616 (71.56%)条isoform得到注释。进一步高级注释共获得21 848个蛋白编码序列, 共预测到537个转录因子、6 430个长链非编码RNA和10 060个SSRs位点。KEGG通路分析发现两条可能与劳盆拟刺铠虾适应深海化能极端生境相关的通路, 分别为过氧化物酶体通路和谷胱甘肽代谢通路。其中, 共有180条isoform被发现参与编码过氧化物酶体通路中的31个关键酶, 213条isoform参与谷胱甘肽代谢通路中19个关键酶的编码。基于全长转录组数据, 利用同源比对、功能结构域预测及系统发育树构建等方法对劳盆拟刺铠虾谷胱甘肽S-转移酶(GST)基因家族进行挖掘与鉴定, 共发现属于theta、delta、mu、kappa四种亚家族的20条GST候选序列。通过对劳盆拟刺铠虾全长转录组测序、功能注释和环境适应相关的重要基因及通路的分析, 不仅丰富了深海组学数据资源, 也为进一步揭示甲壳动物适应深海化能极端环境的分子机制奠定了基础。     

西太平洋马里亚纳海沟附近海山的金相柳珊瑚属(珊瑚虫纲:软珊瑚目:金柳珊瑚科)二新记录种

为探究热带西太平洋海山生物多样性,2016年利用"发现"号遥控无人潜水器(ROV)对西太平洋马里亚纳海沟附近的M2海山进行了采样调查,其中获得了3个深水金相柳珊瑚样本。通过光学显微镜和扫描电镜观察,鉴定其为黑发金相柳珊瑚Metallogorgia melanotrichos(WrightStuder,1889)和长刺金相柳珊瑚Metallogorgia macrospina Kükenthal,1919。这两种金相柳珊瑚均为该区域的新记录种,对其作了详细的形态描述,并依据特征性状对该属所有物种作了分类检索。     

Estimates of size for the large octopus Macroctopus maorum from measures of beaks in prey remains

Macroctopus maorum, also known as Octopus maorum and Pinnoctopus cordiformis, grows to 12 kg and is the largest octopod in Australasia. In New Zealand, this species features in the diets of seals and albatrosses. Beaks of depredated octopus resist digestion and accumulate in the stomach until regurgitated or defecated. Beaks of M. maorum were differentiated reliably from those of other octopods in prey remains for octopus > c.100 g. Previous studies estimated the size of depredated M. maorum from measures of lower beaks using equations derived either from a small sample of M. maorum or from related species. Here, allometric equations to estimate ventral mantle length (4.6–27.5 cm), dorsal mantle length (5.1–29.0 cm), total length (20–198 cm), and body mass (36–12 000 g) were derived from measures of both upper and lower beaks of 90 specimens of M. maorum. These regressions were imprecise and reflected innate variability in octopus morphometrics, a characteristic reflected in the results of analyses of published data for M. maorum from northern New Zealand and for two other large octopods, Octopus vulgaris and Enteroctopus magnificus.     

Diet and feeding habits of the sperm whale (Physeter Catodon L.) in the cook strait region of New Zealand

Stomachs of 151 sperm whales were examined at the Tory Channel whaling station in 1963 and 1964. In nine stomachs examined quantitatively there was a 1.69:1 ratio of squid to fish by weight. Some gastroliths were found. The weight of fresh food varied from 12.70 to 105 kg.

Two samples of lower mandibles from squids totalling 2,118 specimens were separated into 11 types and subtypes with the help of a previously published key by M. R. Clarke. These types have been designated: Ai, Aii, Aiii; Bi, Bii, Biii; and C, D, E, F, and G. Some beaks (2.36% of the total) could not be grouped into these categories.

Onychoteuthid squid made up the bulk of the stomach contents by number and weight. The following squid were identified: Nototodarus sloanei, Histioteuthis cookiana, Architeuthis sp., and Moroteuthis sp. The most common fish in the stomachs were: Polypriori oxygeneios (groper), Genypterus blacodes (ling), Hoplostethus sp., an unidentified eel, and Jordanidia solandri (southern kingfish). Other species recorded included Zeus faber (John Dory), Koinga kirki (spined dogfish), Myctophus humboldti (lantern fish), Echinorhinus cooki, and Echinorhinus sp. or Dalatias sp. Quantitative estimates suggested that commercial species of fish were usually less than 32% of the contents by weight.

The feeding habits are discussed.     

Temporal and cross-shelf distribution of ichthyoplankton in the central Cantabrian Sea

Environmental variables have been measured and sampling for ichthyoplankton has been conducted monthly, since April 2001, at three stations, located at the inner (1), middle (2) and outer (3) shelf of the central Cantabrian Sea. This paper presents the results of the study of the ichthyoplankton collected from July 2001 to June 2004. Fish larvae from 99 species, belonging to 37 families, were identified. Families with higher number of species were Gadidae, Sparidae and Labridae. The larval fish assemblage was dominated by pelagic fish species, with Sardina pilchardus, as the most abundant. There was a pronounced spring peak in larval abundance, dominated by S. pilchardus. A smaller peak, dominated by S. pilchardus and Micromesistius poutassou, was recorded in late winter at Stns 2 and 3. This pattern was evident for the three-year study. Results also indicate that this study was limited to the coastal larval fish assemblage inhabiting the central Cantabrian Sea shelf. This assemblage was temporally structured into other three assemblages: winter, late winter–spring and summer–autumn. Temperature was apparently a key factor in larval fish assemblage succession. In a scenario of global warming, this study constitutes a basis to evaluating the ongoing changes in the pelagic coastal ecosystem of the central Cantabrian Sea.     

Reproductive biology of Parazoanthus axinellae (Schmidt, 1862) and Savalia savaglia (Bertoloni, 1819) (Cnidaria,Zoantharia) from the NW Mediterranean coast

Despite the wide distribution of zoanthids, little is known about their pattern of reproduction. Here we investigate the reproductive biology of two Mediterranean species, the common Parazoanthus axinellae (Schmidt) and the rare Savalia savaglia (Bertoloni). For both species, samples were collected during an annual cycle, from January to December 2005, in the Western Mediterranean (Ligurian Sea, Italy). Both species are gonochoric. In P. axinellae the sex‐ratio (n colonies = 30) showed a slight predominance of male colonies (M/F = 1.35), whereas in the population of S. savaglia (n colonies = 15) a predominance of females was found (M/F = 0.3). In P. axinellae the first gametocytes were visible in March, whereas in S. savaglia they became visible in May. Both species reproduce at the end of autumn when seawater temperature begins to decrease. Parazoanthus axinellae (10 m depth) spawns eggs and sperms in November, whereas S. savaglia (67 m depth) spawns in December. In P. axinellae sexes were segregated on a rocky wall, with males occurring deeper, whereas male and female colonies of S. savaglia were irregularly dispersed in the population. The maximum number of oocytes differed between the species, being higher in P. axinellae than in S. savaglia.     

Spread and status of seven submerged pest plants in New Zealand lakes

The distribution of seven submerged aquatic pest plants is reported. Lake vegetation surveys recorded pest plants in 27.9% of 344 lakes, with two species co‐occurring in 5.8%, and three species in 2.6% of lakes. Egeria densa was most frequent (15.4% of lakes), followed by Ceratophyllum demersum (9.0%), Lagarosiphon major (7.3%), and Utricularia gibba (5.5%). Spread since 2000 has continued for five pest plants, with 34 lakes invaded by U. gibba over 2004–08 alone. Early regional sites in proximity to human population centres were likely plant liberations and numerous potential founder colonies remain in garden ponds. Human activities were important for inter‐lake dispersal, with the exception of bird‐dispersed U. gibba. Significant lake associations between pest plants, and with presence of six exotic fish species, suggest common dispersal pathways and similar introduction risks. Therefore, predictions of future spread should be possible based on sources, dispersal pathways, and identifying key risk factors for lakes.     

Prevalence of Kudoa thyrsites (Myxozoa) in South African sardine Sardinops sagax,kingklip Genypterus capensis,and Cape hakes Merluccius capensis and M. paradoxus

Prevalence of infection with the myxozoan parasite Kudoa thyrsites is not well documented in sardine Sardinops sagax, the Cape hakes Merluccius capensis and M. paradoxus, and kingklip Genypterus capensis. Quantitative polymerase chain reaction assays were used to determine the prevalence of K. thyrsites in wild-caught S. sagax (n?=?296), Merluccius spp. (n?=?162) and G. capensis (n?=?70), harvested in 2015. Sardinops sagax were harvested from the east, south and west coasts of South Africa, whereas M. capensis, M. paradoxus and G. capensis were taken from the west coast only. Relationships between K. thyrsites infection prevalence and fish capture locality, season, sex and size were determined with Chi-square tests. Prevalence was highest in S. sagax (91%), followed by M. capensis and M. paradoxus (87%) and G. capensis (40%). Prevalence was independent of sex of the host for all fish species. In the case of S. sagax only, the prevalence of infection was independent of locality of capture but depended on season, with prevalence lowest during spring and highest during autumn. No significant relationship (p?>?0.05) was found between infection prevalence and host size for any of the fish species.     

Feeding of hake and a first attempt at determining their trophic role in the South African west coast marine environment

During intensive biannual demersal fish-sampling cruises since January 1983 over the South African west coast shelf region (Orange River to Cape Agulhas, coast to 500-m isobath), feeding studies on both species of Cape hake (Merluccius capensis and M. paradoxus) were undertaken. These studies were of two forms, random sampling of the dietary preference and constituents of the hake caught during daylight only and intensive sampling of stomachs with food throughout a 28-hour cycle. From the index of relative importance, crustaceans were shown to be most important in the majority of length classes of hake studied, with mesopelagics (fish and cephalopods) and other hake also important. Differences by hake species, by predator size, by geographical location and by season were evident. The Cape hakes, which constitute some three-quarters of the demersal fish biomass in the area, are deduced to play a major role in the marine faunal environment, both as predators and prey, and some base-line figures of the quantities of different food items consumed by the resource are presented. Hake are largely opportunistic feeders and it is concluded that they can adapt to perturbations in the availability of prey, whether they be triggered by environmental cause or by, for example, purse-seining of particularly mesopelagic fish.     

几何形态学对8种扇贝的形态分类的应用

几何形态学(morphometrics)是基于笛卡尔地标点的统计分析方法,主要运用广义普鲁克分析(GPA)、薄板样条分析(TPS)、主成分分析(PCA)、典型变量分析(CVA)等方法,定量的对形态变化进行识别,结果更为客观。本研究运用几何形态学方法对8种常见扇贝(栉孔扇贝Azumapecten farreri、虾夷盘扇贝Mizuhopecten yessoensis、海湾扇贝Argopecten irradians、平濑掌扇贝Volachlamys hirasei、北海道扇贝Swiftopecten swiftii、荣套扇贝Gloripallium pallium、华贵类栉孔扇贝Mimachlamys nobilis、美丽环扇贝Annachlamys striatula)间形态关系进行研究,运用界标点和半界标点对扇贝的壳盘和壳耳进行数字化标点,利用PCA、CVA、TPS方法,获得8种扇贝的形态差异并进一步分析,建立系统发育树。PCA和CVA分析结果表明可以通过几何形态测量学分析方法将8种扇贝基于形态差异进行有效区分。此外,结合TPS的分析结果,发现壳耳和壳盘在扇贝中具有种间规律性差异。...     

基于16S rRNA序列初步探讨贻贝属的系统发育

通过比较贻贝属5个物种包括中国的两种贻贝的线粒体16S rRNA基因部分序列,来初步确定它们的系统发育关系和了解中国沿海两种贻贝的遗传多样性情况.以Perna viridis为外群,采用NJ法和MP法构建分子系统树.系统发育分析表明,5种贻贝(Mytilus californianus, M. corcuscus, M. galloprovincialis, M. edulis, M. trossulus)在系统树上依次进行分叉,呈放射状.M. californianus最为原始,M. corcuscus次之.每一个贻贝物种都形成单系.其中,M. edulis和M. trossulus是非常相似的,M. corcuscus和M. californianus的亲缘关系近.同时发现,我国沿海分布的紫贻贝(M. galloprovincialis)和厚壳贻贝(M. corcuscus)的遗传多样性都较高,但厚壳贻贝的遗传多样性要低于紫贻贝,可能是由于厚壳贻贝过度被渔民开采等导致厚壳贻贝群体大小降低的缘故.这里系统发育分析为将来进行物种进化、迁移和育种方面的比较研究提供理论基础.     

基于延绳钓观察员数据的热带太平洋中东部食物网拓扑结构分析

热带太平洋是金枪鱼类、旗鱼类等渔业的主要作业海域,但目前针对此海域食物网的研究仍十分缺乏。本文根据2017年6月-11月中国金枪鱼科学观察员在热带太平洋中东部海域（13°09''N -34°27''S, 100°03''-176°17''W）采集的鱼类样品,结合胃含物及其他分析方法以35个物种间的摄食关系为基础对此海域的食物网结构进行了分析。拓扑学指标（节点强度,D;中心性指数,BC和CC;拓扑重要性指数,TI¹和TI³;关键性指数,K、K_t和K_b）和Key-Player算法（KPP-1和KPP-2）用来筛选关键种,并结合体长数据建立了基于关键种的简化食物网。肯德尔相关性分析和聚类分析结果表明,不同拓扑学指标筛选出的关键种是一致的。大多数关键种与太平洋其他海域的研究结果是相同的,如剑鱼（Xiphias gladius）、长鳍金枪鱼（Thunnus alalunga）、小吻四鳍旗鱼（Tetrapturus angustirostris）头足类和鲭属。移除5个关键种后,食物网将被离散为许多互不连接的部分（F=0.632,F^D=0.795,R^D=0.957）,表明这些物种在维持食物网结构和稳定性中的必要性。物种体长是本研究构建简化食物网时的决定性因素。研究结果有助于提高对热带太平洋食物网结构的认识,同时为进一步的生态系统动力学研究提供科学依据。     

Spawning strategies and transport of early stages of the two Cape hake species,Merluccius paradoxus and M. capensis,in the southern Benguela upwelling system

Seasonal and short-term variability of environmental parameters influence the spawning strategies of fish species. In this study, the spawning strategies and the transport of early stages of the two Cape hake species off South Africa were investigated. Distribution of eggs and larvae of Merluccius paradoxus and M. capensis was analysed in order to derive more detailed and species-specific information on spawning season, spawning location, and transport of early stages. Samples were collected during three pilot surveys between January and October 2007 and during an extensive survey in September/October 2008 in the southern Benguela upwelling system off South Africa. Eggs and larvae of M. paradoxus were found in greater numbers than those of M. capensis during all surveys. Highest abundances were found from September to October, indicating one spawning peak for M. paradoxus during late austral winter to spring. The western Agulhas Bank was identified as the primary spawning ground, and smaller spawning events occurred on the West Coast. Larvae of both species were mainly distributed in subsurface waters between 25 and 100 m. More than 50% of all larvae caught had a total length between 3 and 4 mm and size increased significantly with decreasing latitude. Merluccius capensis were found closer inshore than M. paradoxus, indicating that early stages of the two species followed separate drift routes. We assume that this distribution pattern most likely evolved from differences in spawning location and phenology. The spawning strategies of M. paradoxus and M. capensis are well adapted to a time-frame of optimal transport conditions favourable for larval survival in the highly variable environment of the southern Benguela upwelling system, but the peak spawning of the two species is separated in time and space.     

南极菲尔德斯半岛土壤微生物在富集传代培养中对菲的响应

为揭示富集传代培养过程中南极多环芳烃降解菌群的演替规律,以菲为唯一碳源和能源对南极菲尔德斯半岛不同地理位置的6份土壤样品进行了富集和连续传代培养,并采用高通量测序技术分析了富集传代培养过程中菲降解菌群的群落结构及生物多样性。Alpha多样性分析结果表明,在富集培养阶段南极菲降解菌的物种丰富度显著高于传代培养阶段(P0.05),但传代培养阶段的代际物种丰富度无显著差异(P0.05)。Beta多样性分析结果表明富集培养阶段与传代培养阶段的群落结构具有显著性差异(P0.05),但传代培养阶段菲降解菌群趋于相对稳定,代际间群落结构无显著性差异(P0.05)。大量微生物在富集培养阶段丰度较高,但因不能适应实验室培养条件或不能利用菲而在传代培养阶段被淘汰。反之,假单胞菌属(Pseudomonas)、鞘氨醇菌属(Sphingobium)、贪噬菌属(Variovorax)、甲基娇养杆菌属(Methylotenera)和产碱杆菌属(Alcaligenes)等适应了该选择压力并在传代培养阶段形成了动态平衡的优势种群。此外,Pseudomonas、Sphingobium和Variovorax等在富集传代培养过程中具有显著性差异的优势种在维持群落的动态平衡中也发挥着重要的作用。该研究有助于加深人们对南极土壤环境中持久性有机污染物的降解潜力以及关键微生物动态变化的认识,为下一步充分挖掘利用南极环保功能微生物资源提供重要的依据。     

Response of Acartia populations to environmental variability and effects of invasive congenerics in the estuary of Bilbao, Bay of Biscay

The effect of environmental factors (river discharge, water temperature and dissolved oxygen saturation) on the abundance and distribution of Acartia populations and the interactions between their congenerics was evaluated by means of transfer function (TF) models in the estuary of Bilbao during the period 1998–2005. The recorded species were Acartia clausi, Acartia tonsa, Acartia margalefi and Acartia discaudata. Acartia clausi dominated in the entire euhaline region of this estuary until 2003 when it was displaced from the inner part by A. tonsa. This invasive species (A. tonsa) was found for the first time in 2001 and colonized successfully the inner (salinity 30) and intermediate (salinity 33) waters of this estuary since 2003. The TF models revealed an immediate and negative effect of A. tonsa on A. clausi in the intermediate salinity (33) waters, where these species showed the highest spatial overlap. The results indicate that environmental changes from 2003 influenced the abundance of Acartia species, being unfavourable for A. clausi. The decrease of A. clausi abundance in low salinity waters was related to a significant decrease of dissolved oxygen saturation levels, whereas the increase of temperature was linked to a significant increase of A. tonsa. Acartia margalefi and A. discaudata were scarce over the entire period, but they were found to be valuable indicators of hydrological changes, which were associated to climate factors. These two latter species increased in abundance and expanded their seasonal distribution, and in the case of A. margalefi also its spatial distribution, in 2002, coinciding with the period in the time-series when autumn–winter rainfall and summer temperatures were lowest, and dissolved oxygen saturation levels were highest.     

黑潮入侵对南海东北部浮游植物群落结构的影响

To further understand the effect of Kuroshio intrusion on phytoplankton community structure in the northeastern South China Sea(NSCS, 14°–23°N, 114°–124°E), one targeted cruise was carried out from July to August, 2017. A total of 79 genera and 287 species were identified, mainly including Bacillariophyta(129 species), Pyrrophyta(150 species), Cyanophyta(4 species), Chrysophyta(3 species) and Haptophyta(1 species). The average abundance of phytoplankton was 2.14×10~3 cells/L, and Cyanobacterium was dominant species accounting for 86.84% of total phytoplankton abundance. The abundance and distribution of dominant Cyanobacterium were obviously various along the flow of the Kuroshio, indicating the Cyanobacterium was profoundly influenced by the physical process of the Kuroshio. Therefore, Cyanobacterium could be used to indicate the influence of Kuroshio intrusion. In addition, the key controlling factors of the phytoplankton community were nitrogen, silicate, phosphate and temperature, according to Canonical Correspondence Analysis. However, the variability of these chemical parameters in the study water was similarly induced by the physical process of circulations. Based on the cluster analysis, the similarity of phytoplankton community is surprisingly divided by the regional influence of the Kuroshio intrusion, which indicated Kuroshio intrusion regulates phytoplankton community in the NSCS.     

Ecological consequence of the introduction of the polychaete Marenzelleria cf. viridis into a shallow-water biotope of the northern Baltic Sea

Effects of the North American polychaete Marenzelleria cf. viridis on a simple shallow-water benthic community of the northern Baltic Sea were studied in a field experiment combining natural densities of dominating macrofaunal species. The presence of M. cf. viridis increased benthic production (chlorophyll a) and reduced the survival of the native polychaete Nereis diversicolor. Adult Macoma balthica caused a significant mortality on M. cf. viridis whereas adult Cerastoderma glaucum had no effect on M. cf. viridis. We suggest that the competitive interactions between M. cf. viridis and M. balthica are a possible key factor determining the distribution pattern of M. cf. viridis in the Baltic Sea.