AXENIC CULTURE OF FREE-LIVING CONCHOCELIS OF PORPHYRA YEZOENSIS AND PORPHYRA HAITANENSIS

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USE OF RAPD FOR DETECTING AND IDENTIFYING PORPHYRA (BANGIALES, RHODOPHYTA)

imDUCnONManymolectilarbiologybasedDNAtecboqUesPbodepowrfulmeansforstUdvingofgeneticvariationandphylDgenehcrelationships.AmoleculartecboqUebasedonthePOl}-menachainreaction(PCR)isaneffeChvetoolforraPididentificationofgeneticTnarersknownasRandomAInPlifiedPolymorphicDNA(m)(Wlliamsetal.,l99o;Welsh&McClelland,l99o).Sucheders,derivedfromPrindngsitesrandoInldistributedthIDughoutthegenome,areinheritedinaMendelianPatem.ThesePOlyTnorphismsallowtheanalsisofcomplexgenomeswithoutPriorknoWl…     

SUBUNIT COMPOSITION AND CHROMOPHORE CONTENT OF R-PHYCOERYTHRIN FROM PORPHYRA HAITANENSIS

R-phycoerythrin from Porphyra haitanensis exists in two aggregaion states with different molecularweights. A more highly aggregated form, RPE I,Was chromatographed on Bio-Rex 70 column with ureasolution (pH 3.0) as eluent, and the molecular weights of the 3 subunits (α,β,γ) obtained were determinedon SDS-PAGE at 18000, 19200 and 30000, respatively. α subunit carried two phycoerythrobilin (PEB),β subunit, three PEB and one phycourobilin (PUB),γ subunit, one PEB and three PUB chromophores.The molar ratio of α,β, and γ subunits of RPE I wn 6:6:1, and their subunit composition was con-fired to be (αβ)_6γon account of the molecular weight of RPE I, 232000.A lower aggregated form, RPE Ⅱ, contained α and β subunits similar to those of RPE I, but itssubunit composition was the (αβ) monomer of RPE.     

EFFECT OF CYTOKININS AND AUXINS ON THE GROWTH OF FREE-LIVING CONCHOCELIS OF PORPHYRA YEZOENSIS

IAA 3-Indolylaceic acid, NAA a-Naphthylacetic acid and Cytokinins in PESI culture mediumwere used in a study on the effects of plant hormones on the growth of free-living conchocelis ofPorphyra yezoensis which showed that its growth in medium with cytokinins, IAA and NAA was morerapid than that in medium with non-phytohormones, that the optimal concentrations for promotinggrowth were 10μg/L for IAA and ZA (Zeatin), and 0.1 μg/L for BA 6-Benzyl amino purine and KIN6-Furfurylamino- purine. Mix use of NAA, IAA and cytokinins, NAA/ZA 1-1000/1μg/L, NAA/BA10/1-1000μg/L, NAA/KIN 1/1-1000μg/L promoted growth. IAA/ZA 0.1 - 1/0.1 - 1 μg/L, IAA/BA0.1 - 1/0.1 - 10 μg/L IAA/KIN 1/0.1 - 1000μg/L also promoted growth.     

COMPARATIVE STUDIES ON STRUCTURAL FEATURE OF AGAR POLYSACCHARIDES FROM PORPHYRA HAITANENSIS GROWN IN SOUTH AND NORTH CHINA

The structural feature of agar polysaccharides from Porphyra haitanensis grown in south China and transplanted to the north was investigated by fractioriation on DEAE - Sephadex A 50, chemical analysis, and infrared and 13C - NMR spectroscopy.The agars composed mainly of Charged molecules were eluted from DEAE - Sephadex A 50 with 1.0 mol/L- NaCl solution from the southern P. haitanensis and with 0.5 mol/L NaCl from the northern one. The 13C-NMR spectra showed that agarobiose [(1 -3)- B D - galactopyranosyl - (1 -4)-3,6-anhydro-a-L-galactopyranose] and the biological precursor of agarobiose [(1-3} B-D-galactopyra nosyl- (1-4)- 6- sulfate-a- L-galactopyranose ] were the major disaccharide repeating units in the charged fractions. The content of the biological precur sor in the agar polysaccharides from southern P. haitanensis was higher than that in the northern one, the content of the biological precursor extracted from cold water was higher than that from hot water, and the content of 6- OMe-D-galactos     

STUDIES ON INTERCROPPING OF PORPHYRA HAITANENSIS T.J.CHANG ET B.F.ZHENG WITH BAY SCALLOP ARGOPECTEN IRRADIANS LAMARCK

Results of the experiment of northward transplantation of Porphyra haitanensis showed thatintercrop of P. haitanensis with Bay Scallop is feasible because the growth duration of both of them is about the same in August - December.Full use of cultivating area, lowering its cost and increasing its product per unit area as well as improving the ecological envronment are realized during intercrop.     

Development of suspended conchocelis ofPorphyra haitanensis (Bangiales, Rhodophyta)

This Mar. 1993 to Aug. 1994 study on suspended conchocelis ofPorphyra haitanensis showed that there were three patterns for development of vegetative filaments: filaments to filaments by “budding”; filaments to sporangial branchlets by “budding”, or cell swelling. There were also three patterms for sporangial branchlet development: vegetatively propagating, changing into conchospores, or dying. Each developmental stage had one or more different developmental directions between vegetative filaments and sporangial branchlets. Developments from conchosporangial branchlets to conchospores were sequential and irreversible. Although sporangial branchlets formed at 29°C could give rise to filaments, they could not propagate as healthily under the same conditions as those formed at 25°C did. Probably the crucial period of plant cell differentiation is in the late stage of sporangial branchlets. In line with the developmental directions of different stages, the authors regulated the development of conchocelis to get ideal different developmental stages materials to obtain very developmentally homogeneous stages, including filaments and sporangial branchlets. Contribution No 3283 from the Institute of Oceanology, Chinese Academy of Sciences. Study supported by the Climbing Project B PD-B6-4-2 of the State Science and Technology Commission of China.     

Formation of primary pit connection during conchocelis phase of Porphyra yezoensis （Bangiophyceae, Rhodophyta）

1 INTRODUCTION Pit connection is an important feature in Rhodophyta and significant in algal phylogeny. Various documents have illustrated the existence of pit-connection in red alga previously (Migita, 1967; Ramus 1969a, b; Bourne et al., 1970; Lee and F…     

越南紫菜果孢子放散规律及壳斑藻的培育初步研究

报导了越南紫菜果孢子的放散规律及其壳斑藻的培育情况,并对该种紫菜的人工移植进行了初步探讨。     

STUDIES ON COLOR TYPE VARIANTSFROM MUTAGENIZED PROTOPLASTS OFPORPHYRA HAITANENSIS CHANG ET ZHENG& P. YEZOENSIS UEDA (RHODOPHYCEASE )

Isolated protoplasts from thalli of Porphyra haitanensis and Porphyra yezoensis were treated with colchicine or irradiated by ultraviolet (UV ). Several types of color variants were observed among the protoplast offspring. After treatment with colchicine: (1) 0.04-0.09% of red type variants in P. haitanensis were obtained; (2) The rate of red type variants and the variegated chimeral thalli composed of red type and wild type of sectors were 6.31- 1.11% in P. yezoensis. After irradiation with UV: (1) 3.5- 10.5% of red type variants in P. yezoensis were obtained: (2) 0.5-2-0% of red type variants and the variegated chimeral thalli composed of red type and wild type of sectors were obtained in P. haitanensis. Colchicine and UV's mutangenic effects on P. yezoensis protoplasts were stronger than those on P. haitanensis protoplasts. The most efficient concentration of colchicine was 0.05%. The optimal length of UV-radiation was 1/2 min (radiation distance 5 cm). The red type variants induced, by colchicine tr     

REPRODUCTIVE MORPHOLOGY OF EUCHEUMA GELATINAE (ESPER) J. AGARDH (SOLIERIACEAE, GIGARTINALES, RHODOPHYTA)

Eucheuma gelatinae (esper)J.Agardh has vegetative and reproductive features distinguishing it fromother species of Eucheuma.The occurrence of reproductive nemathecia containing carpogonial branches,auxiliary cells and post-fertilization stages including gonimoblast and pericarp initiation,fusion cell foram-tion stages and carposporophyte development are described and ilustrated for the first time for this species.     

LABORATORY OBSERVATION ON SPAWNING,FECUNDITY AND LARVAL DEVELOPMENT OF AMPHIOXUS (BRANCHIOSTOMA BELCHERI TSINGTAUNESE)

Although amphioxus is widespread in temperate and tropical seas,its population is diminishing be-cause of environmental pollution.To keep the population of this evolutionarily important animal fromdiminishing,study on its reproduction and development is necessary.The main findings in this study onthe spawning and fecundity of the amphioxus reared in laboratory and its larval development are as fol-lows.1.Water temperature markedly affected the spawning.It spawned only when water temperature reached 21℃.2.Spawning of the amphioxus in laboratory was markedly extended.Initially,the amphioxus spawned at about 7:00 PM,but spawning time was postponed as spawning days went on.3.The number of eggs produced by a female ranged from 1400 to 12800,average of 5800.This al-so represents the fecundity of the amphioxus because it shedded all eggs within the ovary at a time.4.During the first few months of life of the amphioxus,its growth rate changed seasonally.Thegrowth rate in summer and fall was greater than that in     

A NEW“TETRASPORANGIAL”SPECIES OF LIAGORA(RHODOPHYTA,NEMALIALES)FROM HAWAIT

A new species of Liagora,L.perennis sp.nov.from Hawai’i,is described.Unlike dpecies of this genus in China,it is carpotetrasporangial,and at the place of expected carposporangia bears cruciately di-vided tetrasporangia,a detail which differentiates it from the usual species of Liagora without tetraspores that have been reported in China.Notes are provided on other Liagora species that have an impact on the taxonomy of species found in China.     

Cloning and Analysis of Calmodulin Gene from Porphyra yezoensis Ueda (Bangiales, Rhodophyta)

In order to understand the mechanisms of signal transduction and anti-desiccation mechanisms of Porphyra yezoensiss,cDNA and its genomic sequence of Calmodulin gene (CaM) was cloned by the technique of polymerase chain reaction (PCR) based on the analysis of P. yezoensis ESTs from dbEST database. The result shows that the full-length cDNA of CaM consists of 603 bps including an ORF encoding for 151 amino acids and a terminate codon UGA, while the length of genomic sequence is 1231 bps including 2 exous and 1 intron. The average GC content of the coding region is 58.77%, while the GC content of the third position of this gene is as high as 82.23%. Four Ca2+ binding sites (EF-hand) are found in this gene. The predicted molecular mass of the deduced peptide is 16688.72 Da and the pI is 4.222. By aligning with known CaM genes, the similarity of CaM gene sequence with homologous genes in Chlamydomonas incerta and Chlamydomonas reinhardtii is 72.7% and 72.2% respectively, and the similarity of the deduced amino acid sequence of CaM gene with homologous genes in C. incerta and C. reinhardtii are both 71.5%. This is the first report on CaM from a species of Rhodophyta.     

抓住西部开发机遇确保广西地勘经济可持续发展

中央实施西部大开发战略,为西部经济发展带来了一次千载难逢的机遇。作为调整和发展中的广西地勘局,必须抓住这一历史机遇,实现地勘经济的可持续发展。     

HYPOGLOSSUM FUJIANENSIS SP. NOV. (DELESSERIACEAE,RHODOPHYTA) FROM FUJIAN COAST, CHINA

lNwnDUCnoNTheDelesseriaceangenusHopdeumKtitzingincludesabout25speciesoccurringintropicalandtemPerateseasthroughouttheworld-ThereareanuInberofmorphologicalandtaxonodricstudiesonHopghsuminconteInPOngliteratUre(WOInrsleyandShep-ley,l982;Mikdri,l985;WynnandKraf,l985;WynneandBallantin,1986;Yoshi-daandMibo,l986;Miknd,l987;Wynneetal-,'l989).Uptodate,onlyonespecies,H-attenuatam,hasbeendescrihedfrOInChineseseas(Tsengetal.,1983).DuringataXodricstUdyoftheChineseIhadesum,specimenscollectedb…     

从新马泰旅游业宏观现状谈广西旅游业可持续发展

新马泰旅游资源丰富 ,旅游业各具特色 ,广西应学习他们的先进经验 ,保护旅游资源 ,加强环境管理 ,拓宽旅游新领域 ,实现旅游业可持续发展     

Observation of female gamete （Carpogonia） germination of Porphyra yezoensis and P. oligospermatangia

Several papers have reported that part or whole leafy thallus seemingly consisting of zygotospores can give rise to both blades and conchocelis in the same culture of Porphyra. Study on samples of wild and cultivated Porphyra yezoensis and P. oligospermatangia were conducted to clarify the origination of the young blades in the culture. It is confirmed that single cells on the blade of both species, which normally intermixed with zygotospores, germinated into young blades. TEM and SEM observation has shown that the single cells of Porphyra yezoensis had typical features of female gamete （carpogonia） but archeospore. Therefore, the female gametes are responsible in developing leafy thalli.     

地质旅游资源的开发与保护

地质旅游资源是以地质遗迹为依托的旅游资源 ,开发地质旅游资源可以提高人们的生活质量 ,促进经济发展。必须实行“在保护中开发 ,在开发中保护”的总原则 ,实现地质旅游资源的可持续发展     

西部大开发与广西矿业发展

矿业是广西国民经济的支柱产业 ,在西部大开发中 ,必须认清矿业发展面临的形势 ,研究对策 ,才能作出新贡献。