Age determination and growth rate of <Emphasis Type="Italic">Mactra chinensis</Emphasis> (Bivalvia: Mactridae) by external rings and chondrophore growth bands

Age, growth and mortality of Mactra chinensis were investigated during the period from October 2012 to September 2013 in Busan, South Korea. The monthly variation of the marginal index (MI) of the shell and chondrophore showed that the ring of this species was formed once a year during July. We estimated the age of M. chinensis by reading the external rings on the shell and the growth bands of the chondrophore to compare growth parameters between the two growth characters. The age of this species ranged from 0 to 8 years (shell-based age reading) and from 0 to 10 years (chondrophore-based age reading). Based on external rings and growth bands of chondrophore for the same period, the von Bertalanffy growth functions were expressed by the equation, L _t = 101.53[1-exp {-0.15(t + 0.75)}] and L _t = 90.03[1-exp {-0.20(t + 0.50)}], respectively. The likelihood test showed that there was a significant difference in L _∞ (P < 0.001), K (P < 0.001), to (P < 0.001) estimated from non-linear regression between the two growth characters.     

Age and growth of redeye round herring Etrumeus whiteheadi off the South African coast

Ages of redeye round herring Etrumeus whiteheadi were estimated with associated errors and used to infer life-history information, such as age composition, age-at-maturity and instantaneous mortality rate. Samples were collected in November 2005 during a research survey aimed at estimating the biomass of spawning pelagic fish off South Africa’s west and south coasts. Replicate age estimates obtained from sagittal otoliths were collected with slight bias and relatively high precision. A von Bertalanffy model describing growth of the combined sexes, including juveniles, was L_t = 20.41(1 ? e^{?0.41(t ? 1.92)}). Kimura’s likelihood ratio test revealed no statistically significant differences between growth parameters of males and females. Results suggested that otolith length is a better predictor of age than otolith weight. Maturity estimates for E. whiteheadi were similar to those previously documented.     

Age and growth of Cape stumpnose Rhabdosargus holubi (Pisces: Sparidae) in the Eastern Cape,South Africa

Rhabdosargus holubi is a small (maximum weight=2.4?kg) yet important fishery species in the estuaries of the south-east coast of South Africa. Little is known of its biology and specifically its growth rate, which is essential for sustainable management of the fishery. We examined and counted the opaque zones in the sectioned otoliths of 134 R. holubi to determine its age and growth parameters. The otoliths from two recaptured fish marked with oxytetracycline confirmed that one opaque zone was deposited annually. The species reached a maximum age of 18 years and growth was adequately described by a von Bertalanffy growth function of the form: L_t = 358.1 (1 – e^{?0.24(t+0.77)}) mm fork length. There were no significant differences between any of the male and female growth parameters (likelihood ratio test: p = 0.3). The growth was slow (omega index: ω = 86.56); however, despite this, the unique life history of R. holubi may provide a degree of resilience to heavy fishing pressure in estuaries.     

Age, growth and reproduction of the sand smelt Atherina boyeri Risso, 1810 in the Gomishan wetland – southeast Caspian Sea

A total of 2256 specimens of Atherina boyeri caught in Gomishan wetland (a marsh lagoon located at the southeast Caspian Sea) during spawning season from February to August 2007 were examined for life-history attributes. The population has a 4-year life cycle. Length–weight relationship was estimated as W = 0.0053TL^3.0181 for males and W = 0.0050TL^3.063 for females, being allometrically positive for both sexes. The von Bertalanffy growth function fitted to back-calculated size at age data was: Lt = 155.17[1 − exp − 0.28(t + 0.738)] and Lt = 162.77[1 − exp − 0.27(t + 0.727)] for males and females respectively. The sex ratio was 1:1.30 in favor of females. The reproductive season, evaluated from GSI, extended from March to July, with a peak in March. The average absolute and relative fecundities were 2976 eggs and 874 eggs g⁻¹ of body weight respectively. The diameter of oocytes ranged from 0.03 to 0.20 mm with a mean value of 0.68. The life-history patterns of A. boyeri in the population under study imply that the population of this species in the southeast Caspian Sea differs markedly from those of other localities of its range distribution. The differences were thought to be due to differences in geographical locations.     

Age,growth, and sexual maturity of two New Zealand endemic skates,Dipturus nasutus and D. innominatus

Abstract

Rough and smooth skates (Dipturus nasutus (Banks 1841) and/), innominatus (Garrick &; Paul 1974)) were aged by counting growth bands on X‐rays of thick sections of vertebral centra. Band counts were imprecise, but there was no between‐reader bias. Age estimates were not validated. The oldest rough skate was 9 years old, but few were more than 6 years old. Females may live longer than males. The combined sexes von Bertalanffy growth curve was L_r = 91.3 (1 ‐ e^?0.16[^{t + 1.20}]). Half the males matured by c. 52 cm pelvic length (PL) and 4 years, and females by 59 cm PL and 6 years. The oldest smooth skate in the sample was 24 years, but longevity probably exceeds that. Females appear to live longer than males. The combined sexes von Bertalanffy growth curve was: L_t = 150.5 (1 ‐ e^{?0.095[t + 1.06]}). Half the males matured by c. 93 cm PL and 8 years, and females by 112 cm PL and 13 years. Smooth skate are late maturing and long‐lived relative to other skates, whereas rough skate are early maturing with a moderate life span.     

Age and growth of bluenose,Hyperoglyphe antarctica (Pisces: Stromateoidei) from the lower east coast,North Island,New Zealand

Growth of the bluenose, Hyperoglyphe antarctica (Carmichael, 1818) from the lower east coast, North Island, was determined by counting growth check rings in otoliths. One growth check ring appeared to be laid down each year, so this technique is probably valid for aging bluenose. Female fish had a significantly higher growth rate than males. Comparisons of samples from three sites indicated no significant regional differences in growth. The von Bertalanffy parameters L8,K, and t0 fitted to back‐calculated fish lengths were, respectively, 81.1 cm, 0.308, ‐0.627 for males, and 86.1 cm, 0.308, ‐0.384 for females.     

The biology of the catfish Cnidoglanis macrocephalus (Plotosidae) in an Australian estuary

This paper describes the age structure, growth, diet and aspects of gonadal development in the cobbler, Cnidoglanis macrocephalus (Valenciennes), in the large Swan estuary in south-western Australia between August 1982 and June 1984. Analysis of otolith annuli showed that while the 0+ to 3+ age classes were regularly represented in monthly samples, the 4+ and more particularly the 5+ and 6+ were much less abundant. The weighted means for the back calculated lengths at the end of the first to fourth years of life were 181 mm (≡ 26 g), 314 mm (≡ 156 g), 418 mm (≡ 410 g) and 518 mm (≡ 833 g) respectively. The mean length at the end of the second year of life was similar to the minimum legal size for capture by commercial fishermen (320 mm). The von Bertlanffy growth curve calculated from the back calculated lengths was L_t = 917 [1 − e^{−0·20(t + 0·11)}]. The relative weight of mulluscs, crustaceans and polychaetes in the intestine varied markedly between small and large fish, apparently reflecting differences in the size of these prey. The large mean diameter of mature eggs ( ) was correlated with a low mean absolute fecundity (2078). Trends shown by egg size, gonadosomatic index and time of appearance of spent females indicate that spawning takes place between October and December. The attainment of sexual maturity is both age- and size-dependent. Although sexually maturing and occasionally spent fish were found in the lower estuary, meristic values, commercial catch statistics and other data indicate that the cobbler found in the Swan estuary are part of a population which typically spawns at sea.     

Growth,sexual maturity and reproduction in the hottentot Pachymetopon blochii (Val.)

The growth of hottentot is described by the relationship L_f (mm) = 538,015 (1?e^{?0,097(t + 0,431)}). By determining the otolith growth rate and validating it with counts of daily rings identified on scanning electron micrographs of otolith sections, it was found that previous growth curves underestimated the age of the fish by one year. Differences in growth rate between sexes and over geographical regions were negligible. The ratio of males to females in sampled catches was 1:1,383 and 50-per-cent sexual maturity was attained at 220 mm fork length. There are two main spawning seasons, late autumn and summer, although some breeding activity continues throughout the year. Spatial differences in mean gonadosomatic indices were minimal.     

Barium variation in Pagrus auratus (Sparidae) otoliths: A potential indicator of migration between an embayment and ocean waters in south-eastern Australia

Chronological variation in otolith chemistry can be used to reconstruct migration histories of fish. The use of otolith chemistry to study migration, however, requires knowledge of relationships between the chemical properties of the water and elemental incorporation into otoliths, and how water chemistry varies in space and time. We explored the potential for otolith chemistry of snapper, Pagrus auratus, to provide information on movement history between a large semi-enclosed bay, Port Phillip, and coastal waters in south-eastern Australia. Comparisons of water chemistry across two years demonstrated that ambient barium (Ba) levels in Port Phillip Bay were approximately double those in coastal waters (11 μg L⁻¹ versus 6 μg L⁻¹). Ba levels in otolith margins of wild juvenile snapper were highly positively correlated with ambient levels across 17 sampling locations, and levels in otolith margins of adult snapper collected from Port Phillip Bay were approximately double those of snapper collected in coastal waters. Mean partition coefficients for Ba (D_Ba) were similar for juvenile (0.43) and adult (0.46) otoliths, suggesting that otolith Ba incorporation relative to ambient levels was similar across life-stages. Low Ba variation across otoliths from adult snapper maintained in tanks for three years indicated that annual temperature and/or growth cycles did not strongly influence otolith Ba variation. We concluded that chronological Ba variation in snapper otoliths would be a reliable proxy for life-history exposure to variable ambient Ba. We used water chemistry data and Ba levels across otoliths of ocean resident snapper to estimate otolith Ba levels indicative of residence in Port Phillip Bay (>10 μg g⁻¹) or coastal waters (<6 μg g⁻¹). Peaks in Ba exceeding 10 μg g⁻¹ were common across otoliths of snapper collected in Port Phillip Bay and a nearby coastal region. The location of strong Ba peaks within otoliths was consistent with residence in Port Phillip Bay during the spring/summer when snapper move into the Bay from coastal waters to spawn. Our results for snapper support the use of otolith Ba as a proxy for ambient levels throughout the life-history, however, confident interpretation of migration history from otolith Ba chronologies will most likely require matching time series of ambient Ba in the water bodies of interest.     

A preliminary study of age and growth of the monkfish Lophius upsicephalus (Pisces: Lophiidae) on the Agulhas Bank,South Africa

The sagittal otoliths of Lophius upsicephalus, although extremely variable in shape, were found to be the only structures suitable for age estimation. The annuli are multi-ringed zones separated by "wider than normal" opaque zones. They are difficult to interpret, especially those of fish older than nine years. Periodicity of annulus formation could not be determined by standard methods. By inference, annulus formation could be related to feeding habits and reproductive seasonality. The growth in length (sexes combined) is best described by

Length-at-age = 733,7 (1 – e^{?0,1054 (t + 1,879)})mm.

There is a significant difference in growth by mass between the sexes. The respective equations describing growth by mass (in g) for males and females are:

Weight-at-age = 8616 (1 – e^{?0,1054 (t + 1,879)})^2,805 and

Weight-at-age = 9499 (1 – e^{?0,1054 (t + 1,879)})^2,890.     

Aspects of the biology and fisheries of an economically important sparid Dentex macrophthalmus (Bloch 1791) in the Namibe province,Angola

The sparid Dentex macrophthalmus is a widespread, important fishery species along most of the West African coast from southern Namibia to the Mediterranean. In southern Angola it is an important artisanal species targeted predominantly by handline fishers. A biological and fisheries study was conducted on this species in southern Angola between June 2008 and July 2009. It was the dominant species in the artisanal fishery, accounting for 99% of the sparids captured and 67% (by mass) of the total catch. The life history of D. macrophthalmus was characterised by slow growth (females: L_t = 309[1 ? e^{?0.06(t + 5.43)}], males: L_t = 248[1 ? e^{?0.16(t ? 1.77)}]), advanced age at maturity (females: 7.4 years, males: 6.0 years) and high longevity (females: 36 years, males: 38 years). The sex ratio was 1:1 male:female. The length- and age-frequency distributions and macroscopic observations suggested that the species is a late gonochorist. Males and females reached 50% maturity at 151 and 166 mm fork length respectively. Although individuals with ripe gonads were found during most of the year, the peak spawning period appeared to be in December and January. Despite a life history that renders D. macrophthal-mus vulnerable to overexploitation, only 38% of artisanal fishers noticed a decline in the catches of this species. Potential reasons for this include: technology creep; limited pressure on the juvenile portion of the stock as a result of late recruitment (above the length of 50% maturity); the ‘basin affect’, whereby depleted areas are reseeded by areas (e.g. deeper water) inaccessible to the linefishery; and a deep-water reserve of large individuals. It is recommended that precautionary management strategies be implemented until the age estimates are revised by the countries in which this species forms significant fisheries.     

Biology of the redspotted tonguesole Cynoglossus zanzibarensis (Pleuronectiformes: Cynoglossidae) on the Agulhas Bank,South Africa

The biology of the redspotted tonguesole Cynoglossus zanzibarensis, a common African cynoglossid inhabiting the Agulhas Bank, South Africa, is described. Growth studies based on sectioned sagittal otoliths revealed that C. zanzibarensis is relatively fast-growing and long-lived, attaining ages >8 years. Growth in length was rapid in immature fish, fish attaining 56% of their maximum size within their first year. By sexual maturity, fish had attained 28% of their maximum age and 68% of their maximum length. Total length-at-age was best described by the Von Bertalanffy growth model with combined-sex growth described as L_t = 354.78(1?e^{?0.43 (t+1.17)}) mm TL. Sexually dimorphic growth patterns were evident, females attaining larger lengths, but at a slower growth rate than males. Despite the similar mean size of adult fish, the trawl-sampled adult population was dominated by females, with a sex ratio of 1 male:2.4 females. Female C. zanzibarensis mature in their second year of life (275 mm TL), after which they spawn small, pelagic eggs throughout the year. Approximations of the rates of total, natural and fishing mortality were estimated to be 0.62, 0.48 and 0.14 year^?1 respectively.     

Indication of thiols in black sea deep water

Potentiometric titrations of deep Black Sea water give reasonably precise values of sulphide in the concentration range 30–300 μmol l⁻¹ and a strong indication of thiols in the concentration range 10–30 μmol l⁻¹. Organic analysis of Black Sea water should therefore include the search for compounds containing SH groups. A simple stoichiometric model indicates that sulphur-containing proteins might be the main source of thiols after hydrolysis and deamination. The alkalinity and total sulphide are simply related by A_t = 3287 ± 30 + (3.84 ± 0.10) [H₂ S]_t μmol kg⁻¹. The slope of 3.84 instead of the stoichiometric slope of 2.31 indicates a lack of reduced sulphate in the form of hydrogen sulphide.     

Growth of red gurnard (Teleostei: Triglidae) from Pegasus bay,Canterbury, New Zealand

Growth of the red gurnard, Chelidonichthys kumu (Lesson and Garnot), from Pegasus Bay, Canterbury, was measured during 1966–67. Otoliths were used as an indicator of fish growth; mean length‐at‐age data were obtained from back‐calculated fish lengths at the time of formation of successive annual rings in the otoliths. Growth in length was found to be adequately expressed by the von Bertalanffy growth equation :

l_t = 52.0 [1 ‐ e‐^{0.406 (t‐o.291)}]

(where l_t is the fork length in cm at age t). The length: weight relationship was:

w = 78.56 × 10^‐4 l ^3.072

(where w is the weight in grams). From this relationship, growth in weight was described by the equation:

w_t = 1469 [1 ‐ e‐0.406 (t‐0.291)]³.     

Alkalinity titration in seawater: How accurately can the data be fitted by an equilibrium model?

Displaying “calculated minus observed” data for precise titrations of seawater with strong acid permits direct evaluation of important parameters and detection of systematic errors.At least two data sets from the GEOSECS (Geochemical Ocean Sections) program fit an equilibrium model (which includes carbonate, borate, sulfate, silicate, fluoride, and phosphate) within the most stringent experimental error, less than 2 μmol kg⁻¹. The effect of various parameters on the fit of calculated to observed values depends strongly on pH. Although standard potential E₀, total alkalinity A_t, total carbonate C_t, and first acidity constant of carbon dioxide pK₁ are nearly independent, and can be determined for each data set, other parameters are strongly correlated. Within such groups, all but one parameter must be determined from data other than the titration curve.Adding an acid-base pair to the theoretical model (e.g. C_x=20 μmol kg⁻¹, pK_x=6.2) produces a deviation approaching 20 μmol kg⁻¹ at constant C_t; however, adjustment of C_t by about −18 μmol kg⁻¹ to produce a good fit leaves only ± 1.5 μmol kg⁻¹ residual deviation from the reference values. Thus, at current standards of precision, an unidentified weak acid cannot be distinguished from carbonate purely on the basis of the titration curve shape.There are few full sets of numerical data published, and most show larger systematic errors (3–12 μmol l⁻¹) than the above; one well-defined source is experiments performed in unsealed vessels. Total carbonate can be explicitly obtained as a function of pH by a rearrangement of the titration curve equation; this can reveal a systematic decrease in C_t in the pH range 5–6, as a result of CO₂ gas loss from the titration vessel. Attempts to compensate for this by adjustment of A_t, C_t, or pK₁ produce deviations which mimic those produced by an additional acid-base pair.Changing from the free H⁺ scale (for which [HSO₄⁻] and [HF] are explicit terms in the alkalinity) to the seawater scale (SWS) (where those terms are part of a constant factor multiplying [H⁺]) requires modification of the titration curve equation as well as adjustment of acidity constants. Even with this change, however, omission of pH-dependent terms in [HSO₄⁻] and [HF] produces small systematic errors at low pH.Shifts in liquid junction potential also introduce small systematic errors, but are significant only at pH <3. High-pH errors due to response of the glass electrode to Na⁺ as well as H⁺ can be adequately compensated to pH 9.5 by a linear selectivity expression.     

Wave interaction with ‘’-type breakwaters

The wave transmission, reflection and energy dissipation characteristics of ‘’-type breakwaters were studied using physical models. Regular and random waves in a wide range of wave heights and periods and a constant water depth were used. Five different depths of immersion (two emerged, one surface flushing and two submerged conditions) of this breakwater were selected. The coefficient of transmission, K_t, and coefficient of reflection, K_r, were obtained from the measurements, and the coefficient of energy loss, K_l was calculated using the law of balance of energy. It was found that the wave transmission is significantly reduced with increased relative water depth, d/L, whether the vertical barrier of the breakwater is surface piercing or submerged, where ‘d’ is the water depth and ‘L’ is the wave length. The wave reflection decreases and energy loss increases with increased wave steepness, especially when the top tip of the vertical barrier of this breakwater is kept at still water level (SWL). For any incident wave climate (moderate or storm waves), the wave transmission consistently decreases and the reflection increases with increased relative depth of immersion, Δ/d from −0.142 to 0.142. K_t values less than 0.3 can be easily obtained for the case of Δ/d=+0.071 and 0.142, where Δ is the height of exposure (+ve) or depth of immersion (−ve) of the top tip of the vertical barrier. This breakwater is capable of dissipating wave energy to an extent of 50–80%. The overall performance of this breakwater was found to be better in the random wave fields than in the regular waves. A comparison of the hydrodynamic performance of ‘’-type and ‘T’-type shows that ‘T’-type breakwater is better than ‘’-type by about 20–30% under identical conditions.     

Age and growth of filefish, <Emphasis Type="Italic">Thamnaconus modestus</Emphasis> (Günther, 1877) off the Jeju Island of Korea

The age and growth of filefish, Thamnaconus modestus (Günther 1877) in the southern waters of Korea were investigated. Samples were collected with commercial trawl catches during the period from May 2009 to December 2011. Of the 2,626 specimens collected, the sex ratio was not significantly different from 1:1 (P > 0.05). The total length ranged from 11.3 to 42.1 cm. The gonadosomatic index for both sexes was the highest in May to June, indicating that May to June is the main spawning period. The length of females at sexual maturity was 25.92 cm. The length-weight relationship of the filefish was TW = 0.0121TL^3.0536 (n = 1,692, r² = 0.9034, P < 0.001). The age of the sampled individuals was estimated by counting growth rings recorded on the 5^th vertebrae; ages ranged from 0 to 9 years. The filefish of the same age displayed a high individual variation in total length. Length-at-age data were fitted by using the Von Bertalanffy growth model. The estimated Von Bertalanffy growth parameters were L_∞ = 42.04 cm, k = 0.21 year^?1 and t₀ = ?1.56 for females, L_∞ = 41.20 cm, k = 0.18 year^?1 and t₀ = ?2.36 for males, and L_∞ = 43.16 cm, k = 0.17 year^?1 and t₀ = ?2.18 for the combination of both male and female. These data can be used as useful biological information for the future fishery management of filefish resources in Korean waters.     

Water mass ages of coastal ponds estimated using Ra and Ra as tracers

Measurements of the naturally occurring radioisotopes ²²³Ra (t_1/2 = 11.4 days) and ²²⁴Ra (t_1/2 = 3.66 days) in southern Rhode Island salt ponds were combined with a simple model to obtain independent estimates of the age of these coastal waters. Surface water and porewater samples were collected quarterly in Winnapaug, Quonochontaug, Ninigret, Green Hill, and Pt. Judith-Potter Ponds, as well as nearly monthly in the surface water of Rhode Island Sound, beginning January 2002 through August 2003. Surface water activities ranged from 1–78 dpm 100 L^− 1 and 5–885 dpm 100 L^− 1 for ²²³Ra and ²²⁴Ra, respectively. Porewater radium activities ranged from 3 to 715 dpm 100 L^− 1 for ²²³Ra, and 57–4926 dpm 100 L^− 1 for ²²⁴Ra. Results indicate seasonally varying water mass ages for Ninigret (5–12 days), Winnapaug (2–6 days) and Pt. Judith-Potter Ponds (1–9 days) and, in contrast, relatively constant ages for Green Hill (5–7 days) and Quonochontaug Ponds (3–6 days).     

Effects of nitrogen and iron enrichments on phytoplankton communities in the Northwestern Indian Ocean

Nutrient-enrichment bottle experiments in the northwestern Indian Ocean surface waters were conducted to investigate phytoplankton growth following enrichments with either NH₄⁺, NO₃⁻, Fe or Fe + NO₃⁻. Stimulation of phytoplankton growth could be achieved by the addition of either NH₄⁺ or NO₃⁻ under the ambient Fe concentrations, but the most significant increases in Chl a, POC, and cell densities were observed in the Fe + NO₃⁻-amended culture. Iron addition caused more rapid responses of phytoplankton growth in the Fe + NO₃⁻ treatment than those in the NO₃⁻ and NH₄⁻ treatment. However, the Fe-enrichment treatment revealed minimal growth of phytoplankton because of severe major nutrient deficiency and was similar to the control treatment. Increases in the cell density of diatoms and spherical phytoplankton cells (< 10 μm) were significant in the NH₄⁺-enriched samples, whereas NO₃⁻ enrichment alone had little effect on the diatoms. Simultaneous addition of Fe and NO₃⁻ stimulated maximal growth of phytoplankton, in particular in diatoms, coccolithophorids and Phaeocystis type colonies. However, the dominance of coccolithophorids and Phaeocystis type colonies in the Fe + NO₃⁻ treatment may be interpreted as resulting from Si-limitation. The high N/P ratio for phytoplankton nutrient uptake in the N-amended culture indicates the possibility of some P-limited growth. From these results, we conclude that in the northwestern Indian Ocean, Fe and major nutrients are co-limiting phytoplankton production during the northeast monsoon. Iron appeared to affect the ability of phytoplankton to respond quickly to transient nutrient inputs.     

Equilibrium and structural studies of silicon(IV) and aluminium(III) in aqueous solution. II. Formation constants for the monosilicate ions SiO(OH)3 and SiO2(OH)2. A precision study at 25°C in a simplified seawater medium

The hydrolysis of silicic acid, Si(OH)₄, was studied in a simplified seawater medium (0.6 M Na(Cl)) at 25°C. The measurements were performed as potentiometric titrations (hydrogen electrode) in which OH⁻ was generated coulometrically. The total concentration of Si(OH)₄, B, and log[H⁺] were varied within the limits 0.00075 B 0.008 M and 2.5 -log[H⁺] 11.7, respectively. Within these ranges the formation of SiO(OH)₃⁻ and SiO₂(OH)₂²⁻ with formation constants log β₋₁₁(Si(OH)₄ SiO(OH)₃⁻ + H⁺) = −9.472 ±0.002 and log β₋₂₁(Si(OH)₄ SiO₂(OH)₂²⁻ + 2H⁺) = −22.07 ± 0.01 was established. With B > 0.003 M polysilicate complexes are formed, however, with -log[H⁺] 10.7 their formation does not significantly affect the evaluated formation constants. Data were analyzed with the least squares computer program LETAGROPVRID.