Vegetation cutting as a clean-up method for salt and brackish marshes impacted by oil spills: a review and case history of the effects on plant recovery

Manual cutting of marsh vegetation contaminated by oil spills is often debated as a clean-up technique. Cutting oiled marsh vegetation may be proposed to prevent the oiling of sensitive wildlife associated with marshes. Less frequently, oiled marshes may be cut to aid vegetation recovery. This paper reviews several studies of oiled marsh cutting, and presents a case history of the Grand Eagle oil spill, where marsh cutting was used as a clean-up technique. The paper focuses on the effects of cutting on oiled vegetation survival and structural recovery. We conclude that cutting is often detrimental. Marshes should not be cut when impacted by light oils, where high flushing rates are present, or where re-oiling would be likely. Cutting should only be considered for areas where oil may persist, significant impacts to wildlife are likely, and less destructive clean-up techniques have proven insufficient. Seasonality should be considered prior to cutting, since vegetation impacts may be less likely during fall and winter. Concurrent stresses, such as unusual or extreme salinity or hydrological conditions, should also be considered prior to cutting, to avoid stressing oiled vegetation beyond recovery. Finally, if vegetation cutting is used in marsh environments, substrate disturbance should be avoided.     

Use of remote sensing to document changes in marsh vegetation following the Amoco Cadiz oil spill (Brittany, France, 1978)

Image analysis of historical aerial photographs was used to examine the effects of the Amoco Cadiz oil spill and resulting clean-up on marsh ecology. Two heavily oiled marsh systems were compared. The marsh which received no clean-up recovered to its prior condition. The marshes in the system cleaned by sediment removal were extensively altered as a result of changes in intertidal height of the sediment surface.     

Water budget and vertical conductance for Lowry (Sand Hill) Lake in north-central Florida, USA

Water-budget components and the vertical conductance were determined for Lowry (Sand Hill) Lake in north-central Florida, USA. In this type of lake, which interacts with both the surface-water and groundwater systems, the inflow components are precipitation, surface-water inflow, groundwater inflow, and direct runoff (i.e. overland flow), and the outflow components are evaporation, groundwater outflow, and surface-water outflow. In a lake and groundwater system that is typical of many karst lakes in Florida, a large part of the groundwater outflow occurs by means of vertical leakage through an underlying confining unit to a deeper, highly transmissive aquifer called the upper Floridan aquifer. The water-budget component that represents vertical leakage to the upper Floridan aquifer was calculated as a residual using the water-budget equation. For the 13 month period from August 1994 to August 1995, relative to the surface area of the lake, rainfall at Lowry Lake was 1.55 m yr⁻¹, surficial aquifer inflow was 0.79 m yr⁻¹, surface-water inflow was 1.92 m yr⁻¹, and direct runoff was 0.01 m yr⁻¹. Lake evaporation was 1.11 m yr⁻¹, and surface-water outflow was 1.61 m yr⁻¹. The lake stage increased 0.07 m yr⁻¹, and the vertical leakage to the upper Floridan aquifer was 1.48 m yr⁻¹. Surficial aquifer outflow from the lake was negligible. At Lowry Lake, vertical leakage is a major component of the water budget, comprising about 35% of the outflow during the study period. The vertical conductance (K_V/b), a coefficient that represents the average of the vertical conductances of the hydrogeologic units between the bottom of a lake and the top of the upper Floridan aquifer, was determined to be 2.51 × 10⁻⁴ day⁻¹ for Lowry Lake.     

Effects of oil on mangrove seedlings grown under different environmental conditions

The effects of oil treatments (one-time addition of 120 ml per plant and weekly addition of 15 ml per plant) and environmental conditions at time of oiling (air conditioned laboratory/diffuse light vs hot, direct sunlight) on the survival and growth of Rhizophora mangle and Avicennia germinans seedlings were examined. No oiled A. germinans survived longer than a few weeks. Both one-time and weekly oiling depressed survival, stem growth, leaf production and maximum leaf size in R. mangle. Significant interaction terms between oil treatment and environmental conditions occurred later (50 and 59 weeks) for R. mangle stem growth and earlier (21 and 23 weeks) for leaf production. Relative to other treatment combinations, R. mangle mortality was greatest and growth lowest in the combination of one-time oiling under hot, bright outdoor conditions. Results indicate that synergistic interactions between oil effects and environmental conditions may contribute to the wide range of effects reported in the literature in studies of oil contamination of mangroves.     

Firth of Forth oil pollution incident, February 1978

Where seaducks and other waterfowl are highly concentrated, even small quantities of oil can be a major hazard. A pollution incident on the Firth of Forth in February 1978 was notable in that it affected 241 Great Crested Grebes Podiceps cristatus (a high proportion of those present at the time) as well as 700 diving ducks. Evidence is provided indicating that the slick passed close to the main wildfowl feeding area at night, oiling nocturnal-feeding Scaup Aythya marila and Pochard A. ferina and a raft of roosting grebes, while Eider Somateria mollissima and Goldeneye Bucephala clangula, which had moved elsewhere to roost, largely escaped. The behaviour of freshly oiled birds is described. Heavily oiled Pochard flew 7 km inland to a loch before they were incapacitated. This is the sixth oil pollution incident to affect a large number of birds on the Forth since January 1970.     

Gladstone, Australia Field Studies: Weathering and Degradation of Hydrocarbons in Oiled Mangrove and Salt Marsh Sediments With and Without the Application of an Experimental Bioremediation Protocol

This field study was a combined chemical and biological investigation of the relative rates of weathering and biodegradation of oil spilled in sediments and testing the influence of a bioremediation protocol. The aim of the chemistry work presented here was to determine whether the bioremediation protocol affected the rate of penetration, dissipation or long-term retention of a medium range crude oil (Gippsland) and a Bunker C oil stranded in tropical Rhizophora sp. mangrove and Halosarcia sp. salt marsh environments. Permission for the planned oil spills was granted in the Port Authority area of Gladstone, Queensland (Australia). Sediment cores from three replicate plots of each treatment for mangroves and four replicate plots for the salt marsh (oil only and oil plus bioremediation) were analysed for total hydrocarbons (THC) and for individual alkane markers using gas chromatography with flame ionization detection (GC–FID). Sediments were collected at day 2, then 1, 2, 5 or 6 and 12 or 13 months post-spill for mangroves and day 2, 1, 3 and 9 months post-spill for salt marshes. Over this time, hydrocarbons in all of the oil treated plots decreased exponentially. There was no statistical difference in initial oil concentrations, penetration of oil to depth, or in the rates of oil dissipation between untreated oil and bioremediated oil in the mangrove plots. The salt marsh plots treated with the waxy Gippsland oil showed a faster rate of biodegradation of the oil in the bioremediated plots. In this case only, the degradation rate significantly impacted the mass balance of remaining oil. The Bunker C oil contained only minor amounts of highly degradable n-alkanes and bioremediation did not significantly impact its rate of loss in the salt marsh sediments. At the end of each experiment, there were still n-alkanes visible in the gas chromatograms of residual oils. Thus it was concluded that there was unlikely to be any change in the stable internal biomarkers of the oils over this time period. The predominant removal processes in both habitats were evaporation and dissolution, with a lag-phase of 1–2 months before the start of microbial degradation.     

Long-term biological effects of petroleum residues on fiddler crabs in salt marshes

In September 1969, the Florida barge spilled 700,000L of No. 2 fuel oil into the salt marsh sediments of Wild Harbor (Buzzards Bay, MA). Today the aboveground environment appears unaffected, but a substantial amount of moderately degraded petroleum still remains 8-20cm below the surface. The salt marsh fiddler crabs, Uca pugnax, burrow into the sediments at depths of 5-25cm, and are chronically exposed to the spilled oil. Behavioral studies conducted with U. pugnax from Wild Harbor and a control site, Great Sippewissett marsh, found that crabs exposed to the oil avoided burrowing into oiled layers, suffered delayed escape responses, lowered feeding rates, and achieved lower densities. The oil residues are therefore biologically active and affect U. pugnax populations. Our results add new knowledge about long-term consequences of spilled oil, a dimension that should be included when assessing oil-impacted areas and developing management plans designed to restore, rehabilitate, or replace impacted areas.     

Re-evaluating the use of beached bird oiling rates to assess long-term trends in chronic oil pollution

The oiling rate (oiled birds/total birds) has become the international standard to analyze beached bird survey data. However, this index may not reliably track long-term changes in marine oil pollution in regions where other activities that kill seabirds vulnerable to oil, such as hunting and gill-netting, are also changing. We compare the oiling rate from beached bird surveys conducted in southeastern Newfoundland between 1984 and 2006 to an alternative approach, namely trends derived from a model examining the linear density of oiled birds (birds/km). In winter, there was no change in the oiling rate since 1984, while in summer oiling rates significantly increased. In contrast, the number of oiled birds/km showed a significant decline in both winter and summer. The discrepancy in these trends was attributed to steep declines in the number of unoiled birds found in both seasons. In winter, the decline in unoiled birds/km was related to a reduction in the legal murre hunt and less onshore winds, while in summer a reduced cod fishery resulting in fewer murres drowning in nets and warming summers may have lead to the decline. The significant declines in oiled birds/km over the past three decades are hopefully an indication of less oil being present in the marine environment. Although oiled bird densities since 2000 have remained relatively low for the region (winter: 0.58 birds/km, summer: 0.27 birds/km), they still exceed densities reported elsewhere in the world.     

Can biomass burning produce a globally significant carbon-isotope excursion in the sedimentary record?

Negative carbon-isotope excursions have been comprehensively studied in the stratigraphic record but the discussion of causal mechanisms has largely overlooked the potential role of biomass burning. The carbon-isotopic ratios (δ¹³C) of vegetation, soil organic matter and peat are significantly lower than atmospheric carbon dioxide (CO₂), and thereby provide a source of low ¹³C CO₂ when combusted. In this study, the potential role of biomass burning to generate negative carbon isotope excursions associated with greenhouse climates is modeled. Results indicate that major peat combustion sustained for 1000 yr increases atmospheric CO₂ from 2.5× present atmospheric levels (PAL) to 4.6× PAL, and yields a pronounced negative δ¹³C excursion in the atmosphere ( 2.4‰), vegetation ( 2.4‰) and the surface ocean ( 1.2‰), but not for the deep ocean ( 0.9‰). Release of CO₂ initiates a short-term warming of the atmosphere (up to 14.4 °C, with a duration of 1628 yr), which is consistent with the magnitude and length of an observed Toarcian excursion event. These results indicate that peat combustion is a plausible mechanism for driving negative δ¹³C excursions in the rock record, even during times of elevated pCO₂.     

Effect of Bioremediation on the Microbial Community in Oiled Mangrove Sediments

Bioremediation was conducted in the field on a mature Rhizophora stylosa mangrove stand on land to be reclaimed near Fisherman’s Landing Wharf, Gladstone Australia. Gippsland crude oil was added to six large plots (>40 m²) and three plots were left untreated as controls. Bioremediation was used to treat three oiled plots and the remaining three were maintained as oiled only plots. The bioremediation strategy consisted of actively aerating the sediment and adding a slow-release fertilizer in order to promote oil biodegradation by indigenous micro-organisms. Oil addition stimulated the numbers of alkane-degrading bacteria slightly to levels of 10⁴–10⁵/g sediment. Bioremediation of the oiled sediment had a marked effect on the alkane-degrading population, increasing the population size by three orders of magnitude from 10⁵ to 10⁸ cells/g of sediment. An effect of bioremediation on the growth of aromatic-degraders was detected with numbers of aromatic-degraders increasing from 10⁴ to 10⁶ cells/g of sediment. Active aeration and nutrient addition significantly stimulated the growth of hydrocarbon-degraders in oiled mangrove sediment in the field.     

Glacial geomorphology and ice ages in Tibet and the surrounding mountains

Abstract   Since 1973 new data have been obtained on the maximum extent of glaciation in High Asia. Evidence for an ice sheet covering Tibet during the last glacial period means a radical rethinking about glaciation in the northern hemisphere. The ice sheet's subtropical latitude, vast size (2.4 million km²) and high elevation (6000 m a.s.l.) are supposed to have resulted in a substantial, albedo-induced cooling of the Earth's atmosphere and the disruption of summer monsoon circulation. Moraines were found to reach down to 460 m a.s.l. on the southern flank of the Himalayas and to 2300 m a.s.l. on the northern slope of the Tibetan Plateau, in the Qilian Shan region. On the northern slopes of the Karakoram, Aghil and Kuen-Lun Mountains, moraines occur as far down as 1900 m a.s.l. In southern Tibet, radiographic analyses of erratics suggest a former ice thickness of at least 1200 m. Glacial polish and roches moutonnées in the Himalayas and Karakoram suggest former glaciers as thick as 1200–2700 m. On the basis of this evidence, a 1100–1600-m lower equilibrium line has been reconstructed, resulting in an ice sheet of 2.4 million km², covering almost all of Tibet. Radiometric ages, obtained by different methods, classify this glaciation as isotope stage 3–2 in age (Würmian, the last glacial period, ca  60 000–18 000 years ago).     

Profiles of Fecal Porphyrins in River Otters Following the Exxon Valdez Oil Spill

Median levels of Coproporphyrin III (Copro III) in fecal samples of river otters (Lontra canadensis) collected from an oiled area in Prince William Sound, Alaska, USA, during 1990 were significantly higher than in samples collected from the same oiled area during 1996 (p=0.011, one way analysis of variance), a nonoiled reference area in Prince William Sound during 1996 (p=0.002) and a reference area in southeast Alaska during 1998 (p=0.004). An overall test of significance that combined probabilities from the statistical analysis of this porphyrin study with those from other biomarker studies revealed a significant difference in physiological response of river otters between oiled and nonoiled areas of the Sound for 1990 (p < 0.01). We demonstrated that changes in levels of fecal porphyrins may serve as a biomarker that may contribute to a health assessment of wild river otters.     

Results from a sixteen year study on the effects of oiling from the Exxon Valdez on adult pink salmon returns

For sixteen years following the 1989 Exxon Valdez oil spill adult returns of pink salmon in Prince William Sound, Alaska were monitored to assess spill effects on survival. No evidence of spill effects was detected for either intertidal or whole-stream spawning fish. From 1989 through 2004 mean densities for oiled and reference streams tracked each other, illustrating similar responses of oiled and reference stream adult populations to naturally changing oceanographic and climactic conditions. Hatchery fish strayed into the study streams, but similar incursions occurred in oiled and reference streams, and their presence was compensated for to eliminate their influence on determining the success of the returning natural populations. These results, showing no detectable effects of oiling on pink salmon spawning populations, are supported by published field studies on pink salmon incubation success in oiled streams.     

Effects of prescribed burnings on soil hydrological parameters

Prescribed burning is a forest management tool to reduce forest fire hazards. It is largely applied in the USA and is gaining importance worldwide, particularly in Europe. However, its effects on soils still have to be better understood. This study analyses the effects of two types of prescribed burnings (i.e. low and high burn severities of up to 200 °C and at or above 400 °C) on soil hydrophobicity, infiltration, and water storage capacity of top soils. Prescribed burnings were performed on four different plots in southern and western Catalonia, Spain. Soil samples were collected before and after burning to assess water repellency with the water drop penetration time (WDPT). Three rainfall simulations before burning and three after burning were executed on areas of 1 m², and soil water contents at four to five depths were measured every 4 min during and after rainfall simulations using time domain reflectometry equipment (TDR). Following burning at both severities, water storage capacity of the top soil decreased between 1·7 and 5·4%vol on all four plots. No significant changes in volume flux density and velocity of the wetting fronts were discernible. Water drop penetration times increased moderately at the soil surface of the plots that were exposed to the high burn severity, and decreased slightly when burn severity was low. On two of the four plots the presence of partially moist organic litter prevented the underlying soil from excessive heating. Changes in hydrophobicity and water storage capacity of the top soil did not affect infiltration. Copyright © 2008 John Wiley & Sons, Ltd.     

Aspects of Assaying Sediment Toxicity in Irish Estuarine Ecosystems

The toxicity of surface sediments collected from eight estuarine sites around the Irish coast was assessed in March 1997 using a whole sediment bioassay. Two molluscan species, Tapes semidecussatus (1 + age class) and Scrobicularia plana (unknown age) were exposed to test sediments for 21 days. Survival, morphometric, behavioural and histochemical measurements were taken during the 21 day bioassay. 100% mortality occurred in both species in sediments from one site in Dublin Bay within 18 days of initial exposure. 100% survival of T. semidecussatus was observed in all but four of the test sediments to which it was exposed during this study. The animal condition and wet tissue weight of T. semidecussatus decreased for all sediments during the bioassay. 95% survival of S. plana was observed after 21 days exposure to Youghal (reference) sediment. Condition and wet tissue weight of S. plana increased in all test sediments during the bioassay, however, there was significant variation in wet tissue weight of this species on day 25 of the bioassay in all test sediments. There was significant variation in the burrowing behaviour of S. plana during the bioassay. Lipofuscin accumulation was observed in the digestive diverticula of T. semidecussatus exposed to all test sediments with the exception of Youghal (reference). The potential for both species as bioassay organisms is discussed.     

Microbial modulation in the biomass and toxin production of a red-tide causing alga

The effect of S₁₀, a strain of marine bacteria isolated from sediment in the Western Xiamen Sea, on the growth and paralytic shellfish poison (PSP) production in the alga Alexandrium tamarense (A. tamarense) was studied under controlled experimental conditions. The results of these experiments have shown that the growth of A. tamarense is obviously inhibited by S₁₀ at high concentrations, however no evident effect on its growth was observed at low concentrations. Its PSP production was also inhibited by S₁₀ at different concentrations, especially at low concentrations. The toxicity of this strain of A. tamarense is about (0.95–12.14) × 10⁻⁶ MU/cell, a peak toxicity value of 12.14 × 10⁻⁶ MU/cell appeared on the 14th day, after which levels decreased gradually. The alga grew well in conditions of pH 6–8 and salinities of 20–34‰. The toxicity of the alga varied markedly at different pH and salinity levels. Toxicity decreased as pH increased, while it increased with salinity and reached a peak value at a salinity of 30‰, after which it declined gradually. S₁₀ at a concentration of 1.02 × 10⁹ cells/ml inhibited growth and the PSP production of A. tamarense at different pH and salinity levels. S₁₀ had the strongest inhibitory function on the growth of A. tamarense under conditions of pH 7 and a salinity of 34‰. The best inhibitory effect on PSP production by A. tamarense was at pH 7, this inhibitory effect on PSP production did not relate to salinity. Interactions between marine bacteria and A. tamarense were also investigated using the flow cytometer technique (FCM) as well as direct microscope counting. S₁₀ was identified as being a member of the genus Bacillus, the difference in 16S rDNA between S₁₀ and Bacillus halmapalus was only 2%. The mechanism involved in the inhibition of growth and PSP production of A. tamarense by this strain of marine bacteria, and the prospect of using it and other marine bacteria in the bio-control of red-tides was discussed.     

Taxonomic composition and seasonality of diatoms in three Dinaric karstic lakes in Croatia

The composition and temporal distribution of planktonic diatoms were studied in three small shallow karstic lakes – Modro Oko, Desne, and Kuti – throughout the seasons in 1994, 1995, 1997, and 1998. Physico-chemical parameters did not differ among the three lakes, except for NO₃ concentrations. Altogether, 137 diatom taxa within 41 genera were found in all three lakes combined. The highest number of taxa belonged to the genera Surirella (13), Navicula (12), andCymbella (9). Most taxa were tychoplanktonic, with true panktonic taxa contributing only 14% of the total. Despite their physical-chemical similarity, the lakes showed differences in the structure and dynamics of their diatom communities. Blooms occurred at different times in each of the lakes, and community similarity (Jaccard index) among all three was low (17.6–29.1%). The highest mean values of surface diatom abundance in Modro Oko (2199 cells L⁻¹), Desne (7900 cells L⁻¹), and Kuti (20690 cells L⁻¹) were not significantly different. Margalef's species richness index did not differ either among the lakes or seasonally. Only five diatom taxa in quantitative samples, and 22 taxa in net-samples, were common to all lakes. The most frequent diatoms were Cyclotella striata (56–93%), Fragilaria ulna (50–67%), and Asterionella formosa (27–83%). The most abundant taxa in Modro Oko, Desne, and Kuti were, respectively, Cymbella sp. 1 (402 cells L⁻¹), Cyclotella striata (3000 cells L⁻¹) and Asterionella formosa (20690 cells L⁻¹). Eighteen taxa were recorded for the first time in Croatia: Amphora commutata, Caloneis amphisbaena var. subsalina, C. silicula var. peisonis, Campylodiscus echeneis, Coscinodiscus lacustris, Denticula elegans, Gyrosigma acuminatum var. lacustre, G. distortum var. parkeri, G. fasciola, Navicula menisculus var. menisculus, N. pusilla, N. helensis, Stauroneis parvula var. prominula, Staurosira construens f. subsalina, Stephanodiscus sp. 1, Surirella gracilis, S. ovalis, and S. striatula. The genus Stephanodiscus was recorded for the first time in Croatia.     

Dispersant Use and a Bioremediation Strategy as Alternate Means of Reducing Impacts of Large Oil Spills on Mangroves: The Gladstone Field Trials

Over a three-year period (1995–1998), we studied short-term effects of dispersant use and a bioremediation strategy in two consecutive field trials in sub-tropical Australian mangroves. In each case, weathered oil was applied, and a large spill simulated, in mature Rhizophora stylosa trees around 4–9 m tall. In the first trial, we used Gippsland light crude oil with or without dispersant, Corexit 9527. In the second, a bioremediation strategy followed application of Gippsland oil or Bunker C fuel oil. Bioremediation involved forced aeration with supplemental application of nutrients. Dispersant use had an overall positive benefit shown as reduced tree mortality. By contrast, there was no apparent reduction in mortality of trees with bioremediation. However, one year after oiling, leaf densities of surviving trees were greater in bioremediation plots than in controls, and less in oil-only plots. These and other results have been incorporated into spill response management strategies in Australia.     

Declines in oil-rates of stranded birds in the North Sea highlight spatial patterns in reductions of chronic oil pollution

Strandings of oiled seabirds are used to signal the problem of chronic oil pollution. Species-specific oil rates reflect the risk for marine birds to become oiled at sea. High oil rates were characteristic for seabirds common in areas with frequent oil spills; low oil rates for birds wintering away from the busiest shipping lanes. Declining trends in oil-rates were found, reflecting a reduction in the amount of oil intentionally discharged over the past 50 years. Spatial patterns in the risk to become oiled could be identified, when the winter distribution patterns of the affected birds were incorporated in the analysis. Declines in oil rates were most pronounced in coastal birds. These trends were consistent with tendencies to police nearshore waters more effectively than offshore waters. While levels of chronic oil pollution are much reduced, future emphasis should be to reduce chronic oiling more effectively in offshore waters.     

The toxicity of marine sediments in Victoria Harbour, Hong Kong

When the toxicity of marine sediment in Hong Kong was evaluated, it was found that the seven sediments collected within Victoria Harbour were severely contaminated with heavy metals, at concentrations many times higher than those in sediments collected from outside the harbour. The highest metal content was recorded in site VS14 (located near the airport runway and the industrialized area), with copper, zinc, lead and chromium values of 3789, 610, 138 and 601 mg kg⁻¹ dry wt, respectively. This site also had the greatest alkaline phosphatase activities (15 fluorescent intensity unit g⁻¹ wet wt), the largest number of total coliforms (910 CFU g⁻¹ wet wt) and sulphate-reducing bacteria (8.5 × 10⁴ cells g⁻¹ wet wt), implying that site VS14 was also contaminated with organic matter and nutrients. Sediment bioassays, Microtox and algal tests, demonstrated that sediment elutriates obtained from site VS14 were of greatest toxicity. The EC₁₀ value in Microtox tests was 17% elutriate, and the 96-h IC₅₀ values using Skeletonema costatum and Dunaliella tertiolecta were 40 and 79% elutriate, respectively. No toxic effects were found in sediment samples collected from the control site outside Victoria Harbour. Significant correlations were found between the results of the algal toxicity test (using S. costatum) and the coliform count and metal content of the sediments. The Microtox test was less sensitive than the algal bioassay, and no sediment elutriate, even from the site mostly contaminated by heavy metals, caused more than 50% inhibition of the light-emitting activity of the bacteria. In this study, S. costatum (the diatom) provided a more sensitive and reliable test species than D. tertiolecta (the flagellate) in differentiating the toxicity of marine sediments.