微囊藻胶鞘多糖和水溶性胞外多糖的化学特性

微囊藻(Microcystis)产生大量胞外多糖(EPS),包括包裹在细胞外的胶鞘多糖(CPS)和释放到周围环境中的水溶性多糖(RPS).为探究EPS在蓝藻水华发生中的生理生态学意义,迫切需要了解微囊藻EPS的化学特性.本文从太湖分离群体微囊藻,经过大约18个月实验室培养后,其中一些藻株转变为单细胞形态.选择5株群体藻和4株单细胞藻,比较分析这些藻株EPS的化学特性发现:(1)所有9株藻的EPS均为含有脱氧己糖的酸性杂多糖;(2)所有9株藻的CPS的糖醛酸含量(1.2%~2.1%)均低于RPS的糖醛酸含量(2.4%~6.2%);(3)所有9株藻的EPS均含有乙酰基和硫酸基,其中,每一株藻CPS的乙酰基含量均高于RPS的乙酰基含量,所有群体藻CPS的乙酰基含量(4.1%~6.6%)高于所有单细胞藻CPS的乙酰基含量(2.0%~3.2%).本文进而讨论了EPS化学特性对EPS水溶性和微囊藻群体形成的影响,以及对其生态学作用的影响.在这些化学特性中,乙酰取代基团被认为可能是影响微囊藻EPS生理生态学作用的重要因素.     

温度波动对浮游藻类生长及多糖组成的影响

以铜绿微囊藻、蛋白核小球藻及梅尼小环藻为研究对象,分析了春季藻类演替期间主要门类藻对温度波动的响应,实验在恒温(20℃)和昼夜波动(20±5℃)2种温度模式下测定了3种藻的生长和不同形态多糖含量的变化.结果表明,蛋白核小球藻的生长曲线在不同处理条件下没有显著性差异,铜绿微囊藻和梅尼小环藻的生长曲线在波动温度组显著低于恒定温度组,而且温度波动对梅尼小环藻的生长抑制明显强于铜绿微囊藻.随着实验的进行,3种藻在2个温度处理组中的生长速率逐渐趋近,无显著差异,但是在实验中段3种藻的生长速率对2个温度处理的响应差异显著,其中温度波动对蛋白核小球藻的生长速率起到了显著的抑制作用,对梅尼小环藻起到短暂的显著抑制作用,而对铜绿微囊藻的生长速率无显著性影响,表明铜绿微囊藻对短期(3~6 d)的温度波动表现出较强的适应能力.铜绿微囊藻的游离多糖含量在温度波动条件下显著增加,而固着多糖和总多糖的含量显著减少;蛋白核小球藻和梅尼小环藻多糖的含量没有显著性变化,表明温度波动可能不利于铜绿微囊藻固着多糖的积累.因此,相对短期的温度波动有利于铜绿微囊藻生长优势的确立与维持,而相对长期的温度波动可能会通过影响铜绿微囊藻固着多糖的积累而影响其生长优势的维持.     

滇池微囊藻水华多糖的提取和化学特征

为了研究"水华"微囊藻多糖及其对水体化学和生态特性的影响,以去离子水、pH5的缓冲液和pH9.2的缓冲液为提取液,分别在4℃和80℃条件下从滇池"水华"微囊藻中提取多糖,并对其所提多糖部分化学特征进行分析.结果表明pH9.2的缓冲液所提多糖总得率最高,为2.25%;pH5的磷酸盐缓冲液所提多糖总得率最低,为0.383%.溶解性分析表明,不同提取液所提多糖在所试验的几种溶剂中表现为不溶、分散或部分溶解,未能找到将多糖完全溶解的溶剂.气相色谱和气-质联用分析表明,不同提取液所提多糖都含有中性单糖鼠李糖、阿拉伯糖、甘露糖、葡萄糖、半乳糖和一个未知糖.硫酸-咔唑法分析显示不同提取液所提多糖含有糖醛酸.电感耦合等离子光谱显示去离子水4℃条件下所提多糖含有很高的金属元素钙和镁,分别为2.15%和0.4%.初步研究显示,"水华"微囊藻多糖为酸性杂多糖,低溶解性显示在自然水体中它易形成多糖颗粒,有较高含量的金属元素说明它会影响到这些金属元素在滇池中的溶解、沉积、生物利用和循环,特别是钙和镁.     

与微囊藻胞外多糖降解相关的微生物菌群分析

从铜绿微囊藻培养液中提取胞外多糖,化学分析显示其为含8.3%蛋白质的酸性杂多糖.以胞外多糖为碳源接种自然水体的菌群进行富集培养,分析胞外多糖的微生物降解过程及降解菌的组成.结果表明,接种太湖水华期微生物富集菌群后,多糖立即开始被降解,大约在18d后多糖降解过程显著减慢,37d后仍有一部分多糖未能被降解.这些结果说明在自然界中微囊藻胞外多糖是可以被微生物降解的.比较不同水体的菌群对多糖的降解能力后显示,降解菌群只存在于微囊藻水华暴发的阶段.变性梯度凝胶电泳(DGGE)结果显示在整个降解过程中,降解菌群的组成未发生显著变化.对DGGE条带中的DNA片段进行序列分析和系统发育分析表明降解菌群包含以下几类:鞘氨醇单胞菌属(Sphingomonas)3个,褐螺菌属(Phaeospirillum)1个,假单胞菌属(Pseudomonas)1个,红环菌科(Rhodoeyclaceae)1个,Hylemonella(无译名)1个,分枝杆菌属(Mycobacterium)1个.     

光照周期性波动对铜绿微囊藻(Microcystis aeruginosa)和斜生栅藻(Scenedesmus obliquus)生长的抑制

利用自行研制的光生物反应器,以水库中典型的铜绿微囊藻(Microcystis aeruginosa)和斜生栅藻(Scenedesmus obliquus)为研究对象,研究了两种藻类在光照强度周期性波动条件下的抑制特性.结果表明,相对恒定时平均光照强度6100 lx而言,在光照强度周期性波动条件下,在培养至24天时,铜绿微囊藻生物量降低14%、颜色稍黄,斜生栅藻生物量降低24%、藻体严重泛黄;铜绿微囊藻的叶绿素a和类胡萝卜素含量分别降低12.7%和55.5%,斜生栅藻的叶绿素a和类胡萝卜素含量分别降低78%和74%;模拟内波的周期性波动光强下,两种藻的光合作用参数Fv/Fm、YⅡ和ETR下降,热耗散量NPQ增高,且光合作用受到抑制,生长代谢能力减弱,这为内波控藻提供了理论依据.     

温度对铜绿微囊藻(Microcystis aeruginosa)和鱼腥藻(Anabaena sp.)生长及胞外有机物产生的影响

以巢湖优势种铜绿微囊藻(Microcystis aeruginosa)和鱼腥藻(Anabaena sp.)为研究对象,研究不同温度(35、25和10℃)对这两种藻生长特性和胞外有机物产生的影响.结果表明,温度对铜绿微囊藻和鱼腥藻的藻细胞密度、碱性磷酸酶活性和胞外有机物浓度影响显著.25℃是铜绿微囊藻和鱼腥藻最适宜的生长温度,最高细胞密度分别达到3.12×107cells/ml和2.03×107 cells/ml.不同温度下两种藻的碱性磷酸酶活性特征,证实了高温对鱼腥藻生长的抑制和低温对铜绿微囊藻生长的抑制.胞外有机物释放总量受蓝藻生物量和单位细胞有机物释放速率的影响.铜绿微囊藻的溶解性有机碳和胞外总多糖释放量在25℃最高,最大值分别为49.28和38.46 mg/L;而鱼腥藻在35℃时释放量最高,最大值分别为45.82和40.60 mg/L;10℃条件抑制了两种藻的生长及胞外有机物的释放.鱼腥藻胞外多糖含量在35℃培养条件下最高,而铜绿微囊藻在10℃条件下最高,说明不利的生长条件会促进蓝藻胞外多糖的分泌.三维荧光图谱分析结果表明,铜绿微囊藻和鱼腥藻胞外有机物以类蛋白质和类腐殖酸为主,温度主要影响藻细胞胞外有机物浓度,而对有机物种类组成没有影响.     

黑藻(Hydrilla verticillata)对铜绿微囊藻(Microcystis aeruginosa)抑制作用

采用黑藻(Hydrilla verticillata)和铜绿微囊藻(Microcystis aeruginosa)共同培养的实验方法,追踪测定微囊藻的生长量、光合速率等相关生理生化指标以及观察藻细胞超微结构变化,研究黑藻对铜绿微囊藻的抑制作用及其机制．结果表明,黑藻对铜绿微囊藻的生长有明显的抑制作用,表现为藻细胞生长量显著降低,细胞超微结构进行性损伤直至细胞解体,藻体叶绿素a含量和光合速率急剧下降,呼吸速率、超氧物歧化酶(SOD)活性均呈现先升高后下降的趋势,膜脂过氧化产物丙二醛(MDA)的积累量与膜的损伤程度相一致．在黑藻养殖水中初步分离得到黑藻分泌的抑藻物质．     

洱海流域入湖河口湿地沉积物氮、磷、有机质分布及污染风险评价

以云南洱海罗时江河口湿地为典型对象,利用柱状底泥分层采样器采集罗时江河口湿地表层(0~10 cm)沉积物样品,研究分析总氮(TN)、总磷(TP)、有机质(OM)的空间分布特征,并对沉积物进行污染风险评价.结果表明:表层沉积物TP含量在0.04~1.28 g/kg之间,空间分布特征为:Ⅱ区Ⅰ区,水道Ⅰ水道Ⅱ;TN含量在0.33~2.96 g/kg之间,空间分布特征为:Ⅰ区Ⅱ区,水道Ⅰ水道Ⅱ,OM含量在32.43~233.03 g/kg之间,空间分布表现为Ⅰ区Ⅱ区,水道Ⅰ水道Ⅱ.结合综合污染指数与有机指数评价法可知,罗时江河口湿地表层沉积物氮、磷污染:Ⅰ区和水道Ⅰ属于中度污染,Ⅱ区和水道Ⅱ属于轻度污染;有机污染:水道Ⅰ和水道Ⅱ属于重度污染,Ⅰ区和Ⅱ区属于中度污染.罗时江河口湿地表层沉积物空间分布受外源污染物、养殖活动和湿地水生植物的影响,氮、磷外源输入以水道Ⅰ为主,有机质输入以水道Ⅱ为主.     

实时荧光定量PCR方法检测南太湖入湖口产毒微囊藻

南太湖入湖口产毒微囊藻的丰度对于周边县市取水口的水质安全和太湖水质有着重要影响.以藻毒素合成酶基因mcyE/ndaF为靶基因,建立实时荧光定量PCR检测产毒微囊藻的方法,并对南太湖入湖口7个监测点水样中产毒微囊藻的丰度进行检测,结果表明:该实时荧光定量PCR方法的特异性强及准确性、重复性较好.建立铜绿微囊藻(Microcystis aeruginosa)的标准曲线方程为y=-3.454x+49.88,斜率为-3.454,R~2=0.991,扩增效率E为94.6%,定量检测区间为1.689×10~4~1.689×10~8拷贝数/μl.对南太湖入湖口7个监测点检测表明,夹浦和合溪2个监测点的产毒微囊藻数量最高,预测产毒微囊藻浓度分别为(1.99±0.35)×10~5和(1.47±0.23)×10~5cells/ml.7个监测点的产毒微囊藻的种类较为一致,均为铜绿微囊藻.该方法可以快速准确地检测水体中微囊藻毒素产毒藻种种类和数量,为蓝藻水华监测、预警提供技术依据.     

环境因子对三峡库区铜绿微囊藻(Microcystis aeruginosa)群体形成影响及其形态特征

为揭示微囊藻群体形成机理,为有效防治水华暴发提供依据,以三峡库区铜绿微囊藻(Microcystis aeruginosa)为研究对象,研究N、P浓度,Ca~(2+)浓度,光强,温度对铜绿微囊藻生长、胞外多糖(EPS)合成和群体微囊藻形成的影响,并对比分析了单细胞和群体微囊藻的形态特征.研究发现当N≤100 mg/L、P≤5 mg/L时,铜绿微囊藻生物量和EPS合成量随着N、P浓度增加而增加;适宜浓度的Ca~(2+)(65 mg/L)有利于藻生长,EPS产量随着Ca~(2+)浓度增加而降低,过高浓度的Ca~(2+)在刺激微囊藻细胞分泌EPS的同时可能会促进其溶解,Ca~(2+)和EPS均对微囊藻群体形成起桥架粘结作用;光照和温度对EPS合成有一定促进作用,且其促进效果均高于N、P和Ca~(2+)作用,20℃是同时满足微囊藻生长及EPS合成的最有利条件.群体细胞比单细胞周围的胶质鞘更加明显和清晰,多糖胶鞘表面有许多Ca~(2+)晶体,从微观角度可以确定Ca~(2+)在EPS合成及群体形成中起着重要作用.     

基于地图计算的流域水文过程模拟--以太湖流域上游西苕溪流域为例

利用 PcRaster 的环境动态模拟语言，基于地图运算和网格之间水流运动的模拟，开发了水文过程模拟模型，选择 太湖流域上游的西苕溪流域进行试验，用实测资料对模拟结果进行了验证，结果表明模型模拟结果与实际是符合的．本 模型可以用于流域的物质迁移、污染物迁移、人类活动的环境影响变化等的模拟．在此水文过程模拟的基础上，只要叠加 网格上的物质产出率，就可以很容易得到子流域出口的物质通量的变化和其在流域上的空间分布，集成各个子流域过程， 就可以得到流域出口断面的物质通量的变化．     

湖北牛山湖小型鱼类的群落结构及多样性

流域数据模型是流域特征的语义、行为和规则的表达,是进行集成流域模拟和管理的空间数据组织的重要内容．本文以长江三角洲太湖流域上游的西苕溪流域为例,将汇流单元分为自然流域、“大包围”、圩区三种形式,分别建立了各自内部的河湖网络关系,即山区由自然流域单元,内包含水库(或湖泊)、河流、水工点的树状河湖网络关系;平原区由人工汇流单元,内包含大包围、圩区、湖泊、湿地、河道、水工点的网状河湖网络关系,为建立适合我国的流域数据模型做了有益的探索．     

平原圩区磷素流失过程模拟

圩区是太湖流域平原区的主要地理单元,其磷素流失是造成平原区水污染的重要原因之一,定量模拟圩区磷素流失过程是非点源磷污染控制的重要环节.以太湖流域的典型圩区——尖圩为研究对象,根据物质守恒原理构建圩区磷素流失过程模型,模型考虑了圩区自然降雨、人工灌溉、洪涝排水、地面渗漏、作物需水、水面蒸发、沟渠磷素拦截等过程,充分体现了圩区系统磷素流失特征;通过已有研究案例、实地监测与野外调研相结合的方法确定模型参数;模拟结果表明:(1)与太湖流域平原非圩区相比,圩区的年度磷素流失量较低(-0.17~0.54 kg/(hm2·a)),并且年度差异显著;(2)人工灌溉与自然降雨是圩区磷素输入的主要渠道,其磷素输入量分别为0.27~0.69、1.05~1.19 kg/(hm2·a);水体下渗和洪涝排水是圩区磷素的输出途径,其磷素输出量分别为1.04~1.06、0.65~0.93 kg/(hm2·a).     

Modelling hydrological processes and nutrient retention in plain polders

Application of the Soil and Water Assessment Tool (SWAT) model is limited in plain polders, where hydrological and nutrient processes are confined by water conservancy facilities such as dikes and pumping stations. Watershed delineation techniques are proposed to enable SWAT to simulate these processes in a plain polder in Jiangxiang Town (Nanchang County, Jiangxi Province, China). Drainage unit division and multiple-outlet modelling approaches are introduced, and the main river network and land features are incorporated into a digital elevation model (DEM), so that drainage unit delineation can agree well with real flow direction and concentration. The impoundments of pumping stations are regarded as functioning like reservoirs, which are set up in the SWAT model to simulate confined hydrological processes. The results show that confined hydrological and nutrient processes in plain polders are simulated well; the retention rates of total nitrogen and total phosphorus are estimated as, respectively, 39% and 29% in both streams and impoundments.     

Development of a polder module in the SWAT model: SWATpld for simulating polder areas in south‐eastern China

Polders are one of the most common artificial hydrological entities in the plain river network regions of China. Due to enclosed dikes, manual drainage, and irrigation intake operations, polders have had a significant impact on the hydrological processes of these areas. Distributed hydrological models are effective tools to understand and reproduce the hydrological processes of a watershed. To date, however, few models are able to simulate the drainage and irrigation intake interactions of polders at a watershed scale. This study develops a modified version of the Soil and Water Assessment Tool (SWAT) model, which is designed to better represent polders (SWATpld). The SWATpld model simulates drainage and irrigation intake processes by calculating the excess‐water storage in the inner rivers and irrigation schedule for paddy rice in the polder. Both SWAT and SWATpld models were tested for the Liyang watershed. SWATpld outperformed SWAT in simulating the daily discharge and intake of the experimental polder and predicting the monthly peak flow at the outlet of the Liyang watershed, which suggests that the modified model simulates the hydrological responses of the study watershed with polder operations more realistically than the original SWAT model does. Further evaluation at various locations and in various climate conditions would increase the confidence of this model.     

太湖流域省市边界圩区建设问题初探

太湖流域的苏、浙、沪边界地区地处太湖流域下游,地势低洼,水网纵横,是太湖流域洪涝威胁最严重和水环境恶化地区,同时因为地处省市行政区边界,水利矛盾极为突出.解决好边界圩区问题,有利于减轻流域洪涝灾害,促进水资源保护,水环境恢复.本文分析了太湖流域二省一市边界圩区的发展历史和现状,归纳了边界圩区的格局和特点.考虑到防洪、水资源、水环境的影响,从圩区面积、圩内水面率和圩堤线长度几个方面对边界圩区做了研究,给出了边界圩区合适的圩区规模和圩内排涝动力,在此基础上,就边界圩区的建设提出了建议.研究表明,边界圩区的理想规模是400-533hm2,排涝模数为0.83m3/(s·km2).圩区建设应该局部服从整体利益,采用有效的协商机制解决边界圩区的矛盾.     

云南洱海桃溪河口净化工程的设计思路及初步净化效果

以太湖上游西苕溪流域为研究对象,通过分布式水文模型HEC-HMS模拟次降雨洪水过程:采用可视化数据存储系统HEC-DSS建立水文气象数据库,利用Geodatabase地理数据库技术集成流域自然属性数据库,通过距离平方倒数法对雨量数据进行空间插值,SCS曲线数法计算水文损失,运动波法计算直接径流与河道洪水演进,选用基流指数退水法模拟流域基流,并对模型中水库模拟部分进行适当修正.经模型校验,模拟结果表明,计算流量与观测流量拟合较好,效率系数大于0.8,洪峰流量误差低于4%,峰现时间误差低于2 h,该模型在土地利用变化对洪水水文要素的影响研究方面有较好的应用前景.     

Impacts of massive pumping on flood processes in polders—with a case study on Lannihu basin in the Dongting Lake District,China

Flooding risk in polders is dictated by not only rainfall, topography, and land use, but also massive pumping. Unfortunately, existing models are inadequate for resolving floods as water transfer due to pumping is insufficiently accounted for. Here an improved hydrological model (MGB-MP) is proposed under the framework of the large-scale hydrological model (MGB) based on the principle of water balance, explicitly incorporating massive pumping within a polder and also out to external rivers. The proposed model is calibrated and validated for the Lannihu basin, a typical polder with an area of 1353 km² and 126 pumping stations in the Dongting Lake District, China and surrounded by Xiangjiang River and Zishui River. The model performs fairly well, with Nash-Sutcliffe efficiencies concerning water levels over 0.76 for the calibration and over 0.73 for the validation. The model is applied to the Lannihu basin under different pumping station settings and rainfall scenarios to unravel how and to what extent massive pumping affects the flood processes as characterized by water levels and discharge hydrographs. It is shown that massive pumping considerably alters the discharge hydrographs and accordingly leads to substantial decrease in the water levels of rivers, which are independent unit-polders, due to water transfer between unit-polders within the basin and out of the basin. The closer the unit-polders are to pumping stations, the more the water levels in unit-polders decrease. The water levels in unit-polders away from a pumping station is affected by the pumping station capacity to a greater extent than the pumping station's threshold water level for initiating pumping.     

Simulating and understanding effects of water level fluctuations on thermal regimes in Miyun Reservoir

ABSTRACT

Water temperature dynamics in a reservoir are affected by its bathymetry, climatic conditions and hydrological processes. Miyun Reservoir in China is a large and deep reservoir that experienced a large water level decline in 1999–2004 due to low rainfall and relatively high water supply to Beijing. To study changes of stratification characteristics in Miyun Reservoir from 1998 to 2011, the one-dimensional year-round lake model MINLAKE2010 was modified by adding a new selective withdraw module and a reservoir hydrological model. Simulation results under three scenarios demonstrated that the new MINLAKE2012 model accurately predicted daily water levels and temperature dynamics during the water level fluctuation period. The water level decline led to 7.6 and 3.8°C increases in the maximum and mean bottom temperatures and about 29 days reduction in the stratification days. These simulation results provide an insight into the thermal evolution of Miyun Reservoir during the planned future water filling process.

Editor D. Koutsoyiannis Associate editor M. Acreman     

Understanding hydrological processes with scarce data in a mountain environment

Performance of process‐based hydrological models is usually assessed through comparison between simulated and measured streamflow. Although necessary, this analysis is not sufficient to estimate the quality and realism of the modelling since streamflow integrates all processes of the water cycle, including intermediate production or redistribution processes such as snowmelt or groundwater flow. Assessing the performance of hydrological models in simulating accurately intermediate processes is often difficult and requires heavy experimental investments. In this study, conceptual hydrological modelling (using SWAT) of a semi‐arid mountainous watershed in the High Atlas in Morocco is attempted. Our objective is to analyse whether good intermediate processes simulation is reached when global‐satisfying streamflow simulation is possible. First, parameters presenting intercorrelation issues are identified: from the soil, the groundwater and, to a lesser extent, from the snow. Second, methodologies are developed to retrieve information from accessible intermediate hydrological processes. A geochemical method is used to quantify the contribution of a superficial and a deep reservoir to streamflow. It is shown that, for this specific process, the model formalism is not adapted to our study area and thus leads to poor simulation results. A remote‐sensing methodology is proposed to retrieve the snow surfaces. Comparison with the simulation shows that this process can be satisfyingly simulated by the model. The multidisciplinary approach adopted in this study, although supported by the hydrological community, is still uncommon. Copyright © 2007 John Wiley & Sons, Ltd.