Optimization of culturing condition and medium composition for the production of alginate lyase by a marine <Emphasis Type="Italic">Vibrio</Emphasis> sp. YKW-34

Carbohydrases secreted by marine Vibrio sp. YKW-34 with strong Laminaria cell wall degrading ability were screened, and among them alginate lyase was found to be dominant. The effects of medium composition and culturing condition on the production of alginate lyase by marine Vibrio sp. YKW-34 in flask were investigated in this study. In the culture medium of marine broth, no alginate lyase was produced. The activity of the alginate lyase, after being induced, reached 5 UmL^-1. The best inoculum volume and inoculum age were 10% and 12h, respectively. The optimal temperature for alginate lyase production was 25℃. The fermentation medium was composed of 0.5% of Laminaria powder and 0.2% of KNO3 with an initial acidity of pH 8.0. Alginate could induce alginate lyase production but not as efficiently as Laminaria powder did. The addition of fucoidan, cellulose and glucose had negative effect on the alginate lyase production. Other kinds of nitrogen sources, such as yeast extract, beef extract and peptone, had positive effect on the growth of the microorganism and negative effect on alginate lyase production. In addition, the time course of alginate lyase production under the optimized condition was described. The optimal harvest time was 48 h.     

HJ卫星图像水稻种植面积的识别分析

 HJ-1A/1B卫星具有较高时空分辨率,是提取水稻等农作物种植面积的潜力数据源。本文以江汉平原腹地的监利县及周边相邻区域为研究区,根据水稻物候历,选取样区水稻生长关键期的多时相HJ-1A/1B卫星数据,利用水稻移栽期的水分信息和生长期的归一化植被指数(NDVI)变化信息,结合陆表水系数(LSWI)、短波红外波段的反射率(R_IRS-B2)、差归一化植被指数(DNDVI),构建了HJ卫星数据的水稻种植面积识别方法,提取了研究区2009-2011年水稻种植面积,得到面积精度和样本点精度均不小于90%,Kappa值不小于0.80的结果。     

Inter-provincial Differences in Rice Multi-cropping Changes in Main Double-cropping Rice Area in China: Evidence from Provinces and Households

Since the early 1980 s, the multi-cropping index for rice has decreased significantly in main double-cropping rice area in China, which is the primary double-cropping rice(DCR) production area. This decline may bring challenges to food security in China because rice is the staple food for more than 60% of the Chinese population. It has been generally recognized that rapidly rising labor costs due to economic growth and urbanization in China is the key driving force of the ‘double-to-single' rice cropping system adaption. However, not all provinces have shown a dramatic decline in DCR area, and labor costs alone cannot explain this difference. To elucidate the reasons for these inter-provincial distinctions and the dynamics of rice cropping system adaption, we evaluated the influencing factors using provincial panel data from 1980 to 2015. We also used household survey data for empirical analysis to explore the mechanisms driving differences in rice multi-cropping changes. Our results indicated that the eight provinces in the study can be divided into three spatial groups based on the extent of DCR area decline, the rapidly-declining marginal, core, and stable zones. Increasing labor cost due to rapid urbanization was the key driving force of rice cropping system adaption, but the land use dynamic vary hugely among different provinces. These differences between zones were due to the interaction between labor price and accumulated temperature conditions. Therefore, increasing labor costs had the greatest impact in Zhejiang, Anhui, and Hubei, where the accumulated temperature is relatively low and rice multi-cropping index declined dramaticly. However, labor costs had little impact in Guangdong and Guangxi. Differences in accumulated temperature conditions resulted in spatially different labor demands and pressure on households during the busy season. As a result, there have been different profits and rice multi-cropping changes between provinces and zones. Because of these spatial differences, regionally appropriate policies that provide appropriate subsidies for early rice in rapidly-declining marginal zone such as Zhejiang and Hubei should be implemented. In addition, agricultural mechanization and the number of agricultural workers have facilitated double-cropping; therefore, small machinery and agricultural infrastructure construction should be further supported.     

福建晋江沿岸近21年来成片开发土地的时空变化遥感分析

利用遥感技术对经济快速发展的晋江沿岸地区的成片开发土地进行时空变化监测。首先,利用归一化差值不透水面指数(NDISI)提取晋江沿岸成片开发的土地,然后,通过变化监测查明了1987-2008年间晋江沿岸成片开发土地的时空变化。研究表明,近21年来,晋江沿岸成片开发土地规模急剧扩大,其面积增加了18097hm²。研究发现,土地成片开发主要发生在城市沿江的周边地区,特别是晋江下游至河口段两岸。土地利用变化分析表明,成片开发土地主要占用了两岸大量的耕地。最后,对变化的驱动力进行分析,结果显示,晋江沿岸成片开发土地的剧增与当地经济、人口、房地产业的快速发展有密切的关系。     

Effects of environment factors on initiation of sperm motility in sea cucumber Apostichopus japonicus (Selenka)

Sperm of sea cucumber Apostichopus japonicus (Selenka) were quiescent in electrolyte NaCl solution and artificial seawater (ASW) and nonelectrolyte glucose and mannitol solutions when the osmolality was less than 200 mOsm kg-1. The sperm started to be motile as a result of increased osmolality, indicating an osmolality-dependent initiation of sperm motility in sea cucumber. After a brief incubation in hypotonic NaCl and glucose solutions with osmolalities of 200 and 400 mOsm kg-1, sperm lost partial motile ability. Sperm became immobilized when pH was 6.0 in NaCl, glucose and mannitol solutions, suggesting that an H+ release is involved in sperm activation. The decreased pH had no effect on the percentage of motile sperm in ASW, whereas it delayed the time period to reach the maximum motility (motilitymax). Extracellular Ca2+ in electrolyte solutions was not essential for motility stimulation but shortened the time of reaching motilitymax. When Ca2+ was mixed in nonelectrolyte solutions the sperm motility was completely suppressed. The K+ channel blocker, quinine, suppressed the sperm motility in electrolyte solution, showing a possible involvement of K+ transport in the process. High K+ concentration did not affect the sperm motility in NaCl solution, but decreased it in ASW and almost entirely suppressed it in nonelectrolyte solutions. The different effects of pH and K+ in ASW and NaCl solution indicate that external ions may also regulate sperm motility.     

SCREENING FOR ANTIBACTERIAL AND ANTIFUNGAL ACTIVITIES IN SOME MARINE ALGAE FROM THE FUJIAN COAST OF CHINA WITH THREE DIFFERENT SOLVENTS

Three different solvents viz ethanol, acetone and methanol-toluene (3:1) were used to extract antibiotics from 23 species of marine algae belonging to the Chlomphyta, Phaeophyta and Rhodophyta. Their crude extracts were tested for antibacterial and antifungal activities. Among them, the ethanol extract showed the strongest activity against the bacteria and fungi tested. Four species of the Rhodophyta (Laurenc/a okamurai, Dasya scoparia, Grateloupia filicina and plocamium telfairiae ) showed a wide spectrum of antibacterial activity. Every solvent extract from the four species was active against all the bacteria tested. The test bacterium Pseudomonas solancearum and the fungus Penicilium citrinum were most sensitive to the extracts of marine algae. In general, the extracts of seaweeds inhibited bacteria more strongly than fungi and species of the Rhodophyta showed the greatest activity against the bacteria and fungi tested.     

Classification of marine diatoms using pigment ratio suites

Diatoms are widely distributed in many temperate areas and some species frequently form extensive blooms in spring. Hence, monitoring the variations of specific genera or species of diatoms is necessary for studying phytoplankton population dynamics in marine ecosystems. To test whether pigment ratios can be used to identify diatoms at a below-class taxonomic level, we analyzed 14 species/strains of diatoms isolated from Chinese seas using high performance liquid chromatography (HPLC). We normalized all pigment concentrations to total chlorophyll a to calculate the ratios of pigment to chlorophyll a, and calculated the ratios between accessory pigments (or pigment sums). Cluster analysis indicated that these diatoms could be classified into four clusters in terms of three accessory pigment ratios: chlorophyll c2: chlorophyll c1, fucoxanthin:total chlorophyll c and diadinoxanthin:diatoxanthin. The classification results matched well with those of biological taxonomy. To test the stability of the classification, pigment data from one species, cultured under different light intensities, and five new species/strains were calculated and used for discriminant analysis. The results show that the classification of diatom species using pigment ratio suites was stable for the variations of pigment ratios of species cultured in different light intensities. The introduction of new species, however, may confuse the classification within the current scheme. Classification of marine diatoms using pigment ratio suites is potentially valuable for the fine chemotaxonomy of phytoplankton at taxonomic levels below class and would advance studies on phytoplankton population dynamics and marine ecology.     

The effect of nitric oxide on the growth of marine phytoplankton

The incubation experiments of Skeletonema costatum, Dicrateria zhanjiangensis nov. sp., and Platymonas sub-cordiformis, and those of Emiliania huxleyi were carried out in the Marine Physical Chemistry Laboratory in Ocean University of China and in the Marine Organic Geochemistry Laboratory in the University of Georgia respectively. Nitric oxide was added into the media when these marine microalgae were growing. We found the growth of these four microalgae were promoted or inhibited when nitric oxide of different concentrations was added one or two times each day during the cultivation process. The results are consistent with the influence of nitric oxide on the growth of high plants. The results show that nitric oxide may be a new factor of regulation and control for the phytoplankton growth in seawater.     

Comparative Evaluation on Combined Ecotoxic Effects of Nitrofurazone and NH_4Cl Using Two Ciliated Protists as Model Organisms: Population Dynamics and Growth Rates

Ecotoxic effects of antibiotics or ammonium have been confirmed independently in aquatic animals,but few studies have investigated their combined effects.In aquaculture ecosystems,these pollutants frequently coexist,and often in high concentrations.In this study,the combined effects of antibiotic nitrofurazone and NH4 Cl on the population dynamics and growth rates of two species of ciliated protists,Euplotes vannus and Pseudokeronopsis rubra,were investigated.Profiles of the dose-responses were visualized,and interactions between the two pollutants were quantified by the response surface method(RSM).Results showed that 1)the dynamics of the population growth differed significantly between the testd ciliates and varied with the concentrations of the pollutants;2)the relative growth rate(RGR)of both ciliates decreased significantly with increased pollutant concentrations,while the difference in RGR between the two ciliates was not significant;3)RSM analysis demonstrated an additive effect of nitrofurazone and NH4 Cl on the RGR in both ciliates.In brief,ecotoxic effects can be caused by nitrofurazone and ammonium independently on the two test ciliates,and such effects can be strengthened when they present at the same time.These findings offer a valuable reference for evaluating combined ecotoxic effects caused by multiple pollutants in aquaculture ecosystems.     

Adverse Effect of TiO_2 Nanoparticles on Antioxidant System and Antitumor Activities of Macroalgae Gracilaria lemaneiformis

Recently, the widespread use of TiO_2 nanoparticles(nano-TiO_2) has attracted the global concern regarding their adverse effects to aquatic organisms. Nanoparticles may get to the aquatic environments and harm the growth of algae. This paper presents the studies of nano-TiO_2 concentrations influence on the photosynthetic characteristics, antioxidant system and antitumor activity of marine macroalgae Gracilaria lemaneiformis after exposed to 0, 5, 10, 20 or 40 mg L-1 of nano-TiO_2. Results showed that the photosynthetic pigment content of G. lemaneiformis obviously decreased and the antioxidant defense system was significantly disturbed after exposure to nano-TiO_2. Compared with control, the maximum level of malondialdehyde showed 3.3-fold increase for 15 days exposure to nano-TiO_2. The activity of superoxide dismutase and catalase enhanced obviously by 1.10-2.24-fold. The aqueous extract of G. lemaneiformis(AEGL) possesses anticancer activity against HepG2 liver cancer cells proliferation and the 48 h-cell inhibition exceeds 35%. But the toxicity of nano-TiO_2 would inhibit the anticancer activity of AEGL. When G. lemaneiformis was exposed to nano-TiO_2, the effect of the aqueous extract of G. lemaneiformis on the cell inhibition, reactive oxygen species production, apoptosis, cycle arrest of HepG2 cells significantly lessened, and which were linearly associated with nano-TiO_2 exposure concentration. Those results further confirmed that nano-TiO_2 can inhibit the anticancer activity of G. lemaneiformis. The results in this work imply that macroalgae can be an effective biomarker of nano-TiO_2 contaminant, and can be useful indicator to evaluate ecological effects of increasing pollutants in marine ecosystem.     

Dynamics of wetland landscape pattern in Kaifeng City from 1987 to 2002

It is very significant for urban development and urban wetland protection and utilization to probe into the process and reasons of urban wetland landscape dynamics. Taking the information of remote sensing and detailed land survey data as the basic information sources, with the help of RS and GIS, according to the principles and methods of landscape ecology, this paper analyzed wetland landscape pattern dynamics and its reasons in Kaifeng City, Henan Province of China, from 1987 to 2002. The results show that the total wetland area in Kaifeng City firstly reduced by 20.1% from 1987 to 1990 and then increased from 1990 to 2002, with an average annual growth rate of 3.3%. At the same time, landscape fragment degree and landscape dominance degree increased, respectively from 0.64 to 0.72 and from 0.3754 to 0.5563, but mean patch area, maximum patch area, patch fractal dimension reduced. As far as single landscape element concerned, fi＇om 1987 to 2002, patch area, patch number, patch density, patch shape and patch location changed in varying degrees in all wetland types in Kaifeng City, among which rice field changed most and others relatively less. In the recent 20 years, rice fields, lake wetlands, puddles and ponds had a higher stability, but river and bottomland wetlands were mostly transformed to land use types. The change of wetland landscape pattern was the result of the combined action of the Huanghe （Yellow） River, urban expansion, wide cultivation of rice, and rapid development of fishery. Among them human activities were the main driving factors for wetland landscape changes.     

基于MGIS与MODIS SST数据的黑潮海表温度锋特征分析

本文利用我国自主研发的海洋地理信息系统软件MaXplorer,以黑潮为例,阐述了基于过程的海洋形态特征提取方法,利用VC++,对MODIS水星SST时间序列数据进行海表温度锋抽取,并分析了2003年东海黑潮表层温度锋位置的季节性变迁。结果表明,东海黑潮表层温度锋位置的季节性变化不大,只是在台湾东北部海域和北纬30°附近海域季节性变化比较活跃,由于夏季东海表层水温比较均匀,基本保持在28℃以上,温度锋位置难以确定。其他3个季节温度锋的位置由于受海底地形的影响,基本保持在200m等深线附近的陆坡区,而在台湾岛东北部有一个明显的先是气旋,后是反气旋的弯曲,冬季的温度锋位置相对稍偏东,秋季的温度锋路线比较曲折。     

An Investigation on Tolerance and Accumulation of a Facultative Marine Fungus Aspergillus flavus to Pentavalent Arsenic

Tolerance of a facultative marine fungus Aspergillus flavus towards As (V) was tested.Luxuriant growth of the test isolate was observed in culture media with As (V) concentrations of 25 mg L-1 and 50 mg L-1,indicating its tolerance to the metal.Accumulation rate of arsenic was always higher when exposed to As (V) at 50 mg L-1 than at 25 mg L-1.The study reveals Aspergillus flavus as a promising candidate for environmental bioremediation.Arsenic contents (mg g-1) in the fungus when exposed to 50 mg L-1 As (V) were measured as 11.1773,4.0983,and 8.0000 mg g-1 on day 3,6 and 9,respectively.The highest content was observed initially,i.e.on day 3,followed by a decline and a rise again.These results provide baseline information for further explorations regarding the exploitation of the fungus for arsenic removal.     

Enhancing effect of Fe2+ on the formaldehyde production from trimethylamine N-oxide decomposition catalyzed by the extract of Harpadon nehereus kidney

The effects of Fe2+ on the trimethylamine N-oxide (TMAO) demethylating activity of the Harpadon nehereus kidney extract were studied in this research.The activity of the kidney extract was presumably inhibited by ethylene diamine tetra-acetic acid (EDTA),which indicates that the kidney extract contains an enzyme or enzyme system with metal cations as activator.Activity of the kidney extract was enhanced significantly when Fe2+ was added into the model system in vitro.As the concentration of Fe2+ increased,the decomposing rate of TMAO increased rapidly until TMAO decomposed completely.The activity of the kidney extract was also enhanced by reductant such as ascorbic acid.Inductively Coupled Plasma-Atomic Emission Spectrometry (ICP-AES) was employed to determine the content of total iron in a number of fishery products.Significant positive correlation between the contents of total iron and endogenous formaldehyde (FA) was found,especially in marine products.     

THE BETAINES FROM CHINESE SEAWEEDS

Cation-exchange chromatography was selected to extract and separate betaines from marine algae. On the basis of the special chemical characteristics of the betaines and their analogues,Dragendorff‘ s reagent ( KBiI4 ) was used to test the existence of betaines and their analogues in marine algae. The total content of betaines from seven species was obtained by using the Reinkeate salt precipitation method. The results showed that the content of betaines in two species of Chlorophyta and two species of Rhodophyta were relatively high, and that the content of betaines in Enteromopha prolifera could even reach to 0.9%. The content in the three species of Phaeophyta was relatively low.     

基于DMSP/OLS夜间灯光影像的中国东部沿海地区城市扩展动态监测

美国国防气象卫星搭载的业务性线性传感器（DMSP/OLS）所获取的夜间灯光影像数据,能够客观地反映人类对城市建成区的开发建设范围与强度,已广泛地应用于城市扩展的动态监测。本文利用不变目标区域法对长时间序列DMSP/OLS夜间灯光影像进行辐射校正,基于校正后的影像对2001-2013年中国东部沿海地区的城市建成区范围进行提取,结果表明：① 建成区面积从2001年的7550 km²,增加到2013年的21 650 km²,共扩展了14 100 km²,虽然建成区面积呈逐年增加的趋势,但其扩展速率则在逐步减缓,城市重心逐渐向南转移;② 在空间上形成了京津唐、长江三角洲和珠江三角洲3城市群,研究发现京津唐的中小城市面临难以获得发展资源的问题,导致了该地区发展的不平衡;③ 综合分析建成区扩展和经济统计数据,结果表明人口和经济是建成区扩展的主要驱动因子,但同时城市快速扩展也给东部沿海地区带来了一定程度的用地浪费问题;④ 由于DMSP/OLS夜间灯光影像受到自身空间分辨率的限制和灯光过饱和的影响,易造成城市建成区边缘细节部分的错提。新一代Suomi NPP/VIIRS夜间灯光影像在空间和光谱分辨率上均有较大提高,在后续的研究中应充分挖掘其数据优势,以期提供更加精准的城市扩展动态监测。     

A statistical dynamics model of the marine ecosystem and its application in Jiaozhou Bay

Models of marine ecosystem dynamics play an important role in revealing the evolution mechanisms of marine ecosystems and in forecasting their future changes. Most traditional ecological dynamics models are established based on basic physical and biological laws, and have obvious dynamic characteristics and ecological significance. However, they are not flexible enough for the variability of environment conditions and ecological processes found in offshore marine areas, where it is often difficult to obtain parameters for the model, and the precision of the model is often low. In this paper, a new modeling method is introduced, which aims to establish an evolution model of marine ecosystems by coupling statistics with differential dynamics. Firstly, we outline the basic concept and method of inverse modeling of marine ecosystems. Then we set up a statistical dynamics model of marine ecosystems evolution according to annual ecological observation data from Jiaozhou Bay. This was done under the forcing conditions of sea surface temperature and surface irradiance and considering the state variables of phytoplankton, zooplankton and nutrients. This model is dynamic, makes the best of field observation data, and the average predicted precision can reach 90% or higher. A simpler model can be easily obtained through eliminating the terms with smaller contributions according to the weight coefficients of model differential items. The method proposed in this paper avoids the difficulties of obtaining and optimizing parameters, which exist in traditional research, and it provides a new path for research of marine ecological dynamics.     

多时相TM影像决策树模型的水稻识别提取

决策树模型的多时相TM影像的小尺度水稻信息提取在我国还鲜有研究。为此,本文利用水稻生长在潮湿土壤这一特性,选取TM影像中对植物含水量和土壤湿度反应敏感的短波红外波段(1.55~1.75μm),以及反映植物覆盖率、植物长势的红光波段(0.62~0.69μm)和近红外波段(0.76~0.96μm),计算水稻移栽期、灌浆期和成熟期3个时期的归一化植被指数(NDVI)和土壤含水量指数(LSWI),提出一种时间差异的决策树水稻提取模型,以唐山市滦南县南部区域为例开展了研究。经过野外实地验证表明:该模型能有效区分出水域、玉米和菜地等较易与水稻混淆的地物,水稻提取的生产者精度和用户精度分别为95.18%和98.84%,分别比单一时相高出6.78%和7.54%。     

无人机影像的面向对象水稻种植面积快速提取

基于抽样技术的地面调查与遥感影像分类相结合的方法在大范围作物种植面积提取中得到广泛使用。无人机影像具有低成本、高时效、高分辨率的一系列优点,可以快速实现特定区域范围内的农情采样任务。本文以水稻样地为研究对象,采用便携式无人机Mavic Pro进行航拍。对所获取无人机影像进行预处理生成分辨率为3.95cm/pix的正射影像,采用面向对象的思想,目视评价和ESP工具相结合快速选择了最优分割尺度为300,应用了支持向量机、随机森林和最邻近监督分类方法对影像进行了地物分类和水稻面积快速提取。采用目视解译分类结果进行分类结果和面积精度评价,总体精度最高的方法为最邻近分类法,此时水稻分类用户精度为95%,面积一致性精度为99%。研究结果说明了无人机遥感和自动分类能够在平原水稻种植区快速获取样方内高分辨率影像并提取水稻种植面积,弥补了农田被遮挡时地面调查数据的缺失,为大范围水稻种植面积、产量等信息的计算提供样本和验证依据。     

水体中芽孢杆菌和微囊藻的生长及其资源竞争

以C、N、P为主要限制性因子研究了芽孢杆菌和微囊藻在水中的生长率 ,并据Monod方程了解种群增长率与营养浓度之间的关系 ,以判断藻菌资源竞争的结果。研究结果显示 ,氮浓度的升高或磷浓度的降低 ,即N/P比值的升高 ,有利于芽孢杆菌的生长 ;而当氮浓度一定时 ,C/N比值越高 ,芽孢杆菌生长得越好。而微囊藻作为一种自养的光合植物 ,其生长与水中的C源无关 ,与氮磷浓度有关。氮磷浓度越高则生长越好。在此研究结果基础上进行藻菌生长动态的比较表明 ,在氮限制条件下 ,微囊藻的生长率稍高于芽孢杆菌 ;在磷限制条件下 ,芽孢杆菌的生长率总是大大高于微囊藻的生长率