液质联用技术在测定浮游植物色素与粒级分类中的应用

本研究为探讨液质联用技术在测定黄海浮游植物特征色素含量与粒级分类中应用的可行性,在黄海47个站位取表、中、底三层水样进行了分析,对比了超高效液相色谱(UHPLC)和超高效液相色谱串联三重四级杆质谱(UHPLC-MS/MS)两种仪器的色素检测结果。研究发现UHPLC-MS/MS的灵敏度更高,但多数情况下两种方法检测结果吻合度较好,均适用于黄海色素样品的测定。叶绿素a(Chl a)浓度和浮游植物粒级结构空间分析结果显示,山东半岛近岸和长江口东北侧海域,Chl a浓度较高,小型浮游植物占优势;黄海冷水团海域,Chl a浓度较低,微型和微微型浮游植物比例明显升高。该航次的Chl a绝对浓度和不同粒径浮游植物的贡献率与历史数据相比存在一定差异,这与调查时间、站位以及粒级分析方法的差异有关,需进一步比较研究。     

东海、黄海浮游植物生物量的粒级结构及时空分布

浮游植物是海洋初级生产力的主要贡献者,初级生产过程是碳的生物地球化学循环的基础,它启动了海洋生态系统的能量流和物质流,支持着大量的渔业生产量.不仅如此,通过复杂的反馈机制,这一过程还对全球气候变化系统产生深远影响.沿岸海域只占全球海洋面积的8%,但却提供了26%的全球生物生产量和2/3到3/4的世界渔业产量.因此,许多重大的国际研究计划,如海岸带陆海相互作用(LOICZ)、全球海洋生态系统动力学研究(GLO-BEC)等,都将近海浮游植物研究作为非常重要的课题.     

The distribution of phytoplankton size and major influencing factors in the surface waters near the northern end of the Antarctic Peninsula

The waters near the Antarctic Peninsula have always been a study hot spot because of their variable and unique oceanographic conditions.To determine the distribution and possible influencing factors on phytoplankton size and abundance near the Antarctic Peninsula,a large-scale survey was conducted during the austral summer of2018.Samples were collected in 27 stations located in the Drake Passage(DP),South Shetland Islands(SSI),and South Orkney Islands(SOI).Phytoplankton communities were described using chlorophyll a(Chl a),flow cytometry and light microscopy to cover a size range from pico-to microphytoplankton.Nanophytoplankton,especially small nanophytoplankton(2-6 μm) with abundance ranging from 0.66 ×10~3 cells/mL to 8.46 ×10~3 cells/mL,was predominant throughout the study area.Among different regions,there was an obvious size shift.The proportion of picophytoplankton near the Elephant Island(EI) and DP was higher than other regions,and larger cells were found mainly in east of SOI.The distribution of phytoplankton abundance detected by flow cytometry was not completely consistent with Chl a concentrations due to the contribution of larger cells to Chl a.Possible influencing factors on the phytoplankton size distribution were discussed.The properties of water masses such as temperature and salinity can influence the phytoplankton size distribution.Correlation analysis revealed that only picophytoplankton is significantly correlated with salinity.Light and Fe availability might affect phytoplankton abundance and size distribution especially near the waters of SSI and EI in this study.It was also speculated that the abundance of cryptophytes is possibly related to ice melting.     

东、黄海典型海区分粒级浮游植物叶绿素a的周日波动及影响因子

本文分析了东、黄海典型海区3个测站在2000~2003年间4个航次的叶绿素a周日变动特征,结果表明由于地理环境、水文特征以及季节差异,各海区叶绿素a表现出各自不同的变动特点.在东海陆架区,日变化上表层各粒级主要以半日周期为主,受黑潮入侵程度不同而略有变化;长江口由于受到潮汐的影响,各粒级的日变化同潮汐的涨落相对应,主要为半日周期;黄海中部海区叶绿素a尤其是NANO级浮游植物在周日变化上以全日周期为主,受黄海冷水团强弱不同而不同.本文进一步应用渐近回归初步分析了多种环境因子对叶绿素a周日变化的影响.     

The past, present and future of the East/Japan sea in change: a simple moving-boundary box model approach

The East/Japan Sea is a mid-latitude marginal sea that has undergone dramatic changes during the last 50–60 years. One of the most prominent characteristics of these changes is a rapid decrease in the amount of dissolved oxygen in deep waters. As a consequence of these changes, some investigators have even argued that the East/Japan Sea might become an anoxic sea in the next 200 years. While the causes of these changes are still under investigation, it has been shown that they are mainly due to modifications in the mode of the deep water ventilation system in the East/Japan Sea: a slowdown and complete cessation of bottom water formation accompanied by an enhancement of upper water formation instead. A simple moving-boundary box model (MBBM) was developed in order to analyze and quantify the processes involved in such changes over the last 50–60 years. Using a MBBM, we estimated the levels of several conservative chemical tracers (CFCs, Tritium, SF₆, ¹³⁷Cs) and bioactive tracers (oxygen and phosphate) in the deep water masses of the East/Japan Sea, comparing these with the historical data available, and making predictions for the near future. The model predicts that the East/Japan Sea should remain well-oxygenated, despite recent rapid oxygen decreases in its deep waters, accompanied by such structural changes as a shrinking of its oxygen-depleted deeper waters and an expansion of its oxygen-rich upper waters over the next few decades.     

Long-term changes in the fish community structure from the Tsushima warm current region of the Japan/East Sea with an emphasis on the impacts of fishing and climate regime shift over the last four decades

Japanese fisheries production in the Japan/East Sea between 1958 and 2003 increased to their peak (1.76 million tons) in the late 1980s and decreased abruptly with the collapse of Japanese sardine. Catch results for 58 fisheries and various environmental time-series data sets and community indices, including mean trophic level (MTL) and Simpson’s diversity index (DI), were used to investigate the impacts of fishing and climate changes on the structure of the fish community in the Tsushima warm current (TWC) region of the Japan/East Sea. The long-term trend in fisheries production was largely dependent on the Japanese sardine that, as a single species, contributed up to 60% of the total production in the Japanese waters of the Japan/East Sea during the late 1980s. Excluding Japanese sardine, production of the small pelagic species was higher during 1960s and 1990s but lower during 1970s and 1980s. This variation pattern generally corresponds with the trend in water temperature, warmer before early 1960s and after 1990s but colder during 1970s and 1980s. The warm-water, large predatory fishes and cold water demersal species show opposite responses to the water temperature in the TWC region, indicating the significant impact of oceanic conditions on fisheries production of the Japan/East Sea. Declines in demersal fishes and invertebrates during 1970s and 1980s suggested some impact of fishing. MTL and DI show a similar variation pattern: higher during 1960s and 1990s but lower during 1970s and 1980s. In particular, the sharp decline during the 1980s resulted from the abundant sardine catches, suggesting that dominant species have a large effect on the structure of the fish community in the Japan/East Sea. Principal component analysis for 58 time-series data sets of fisheries catches suggested that the fish community varied on inter-annual to inter-decadal scales; the abrupt changes that occurred in the mid-1970s and late 1980s seemed to correspond closely with the climatic regime shifts in the North Pacific. These results strongly suggest that the structure of the fish community in the Japan/East Sea was largely affected by climatic and oceanic regime shifts rather than by fishing. There is no evidence showing “fishing down food webs” in the Japan/East Sea. However, in addition to the impacts of abrupt shifts that occurred in the late 1980s, the large predatory and demersal fishes seem to be facing stronger fishing pressure with the collapse of the Japanese sardine.