Food web flows through a sub-arctic deep-sea benthic community

The benthic food web of the deep Faroe–Shetland Channel (FSC) was modelled by using the linear inverse modelling methodology. The reconstruction of carbon pathways by inverse analysis was based on benthic oxygen uptake rates, biomass data and transfer of labile carbon through the food web as revealed by a pulse-chase experiment. Carbon deposition was estimated at 2.2 mmol C m⁻² d⁻¹. Approximately 69% of the deposited carbon was respired by the benthic community with bacteria being responsible for 70% of the total respiration. The major fraction of the labile detritus flux was recycled within the microbial loop leaving merely 2% of the deposited labile phytodetritus available for metazoan consumption. Bacteria assimilated carbon at high efficiency (0.55) but only 24% of bacterial production was grazed by metazoans; the remaining returned to the dissolved organic matter pool due to viral lysis. Refractory detritus was the basal food resource for nematodes covering ∼99% of their carbon requirements. On the contrary, macrofauna seemed to obtain the major part of their metabolic needs from bacteria (49% of macrofaunal consumption). Labile detritus transfer was well-constrained, based on the data from the pulse-chase experiment, but appeared to be of limited importance to the diet of the examined benthic organisms (<1% and 5% of carbon requirements of nematodes and macrofauna respectively). Predation on nematodes was generally low with the exception of sub-surface deposit-feeding polychaetes that obtained 35% of their energy requirements from nematode ingestion. Carnivorous polychaetes also covered 35% of their carbon demand through predation although the preferred prey, in this case, was other macrofaunal animals rather than nematodes. Bacteria and detritus contributed 53% and 12% to the total carbon ingestion of carnivorous polychaetes suggesting a high degree of omnivory among higher consumers in the FSC benthic food web. Overall, this study provided a unique insight into the functioning of a deep-sea benthic community and demonstrated how conventional data can be exploited further when combined with state-of-the-art modelling approaches.     

First observations of jelly-falls at the seafloor in a deep-sea fjord

Faunal communities at the deep-sea floor mainly rely on the downward transport of particulate organic material for energy, which can come in many forms, ranging from phytodetritus to whale carcasses. Recently, studies have shown that the deep-sea floor may also be subsidized by fluxes of gelatinous material to the benthos. The deep-sea scyphozoan medusa Periphylla periphylla is common in many deep-sea fjords in Norway and recent investigations in Lurefjorden in western Norway suggest that the biomass of this jellyfish currently exceeds 50000 t here. To quantify the presence of dead P. periphylla jellyfish falls (hereafter termed jelly-falls) at the deep seafloor and the standing stock of carbon (C) and nitrogen (N) deposited on the seafloor by this species, we made photographic transects of the seafloor, using a ‘Yo-Yo’ camera system during an opportunistic sampling campaign in March 2011. Of 218 seafloor photographs taken, jelly-falls were present in five, which resulted in a total jelly-fall abundance of 1×10^-2 jelly-falls m⁻² over the entire area surveyed. Summed over the entire area of seafloor photographed, 1×10^-2 jelly-falls m⁻² was equivalent to a C- and N-biomass of 13 mg C m⁻² and 2 mg N m⁻². The contribution of each jelly-fall to the C- and N-amount of the sediment in the immediate vicinity of each fall (i.e. to sediment in each 3.02 m² image in which jelly-falls were observed) was estimated to be 568±84 mg C m⁻² and 88±13 mg N m⁻². The only megafaunal taxon observed around or on top of the jelly-falls was caridean shrimp (14±5 individuals jelly-fall⁻¹), and shrimp abundance was significantly greater in photographs in which a jelly-fall was found (14±5 individuals image⁻¹) compared to photographs in which no jelly-falls were observed (1.4±0.7 individuals image⁻¹). These observations indicate that jelly-falls in this fjord can enhance the sedimentary C- and N-amount at the deep-sea floor and may provide nutrition to benthic and demersal faunas in this environment. However, organic enrichment from the jelly-falls found in this single sampling event and associated disturbance was highly localized.     

Selective uptake of prokaryotic picoplankton by a marine sponge (Callyspongia sp.) within an oligotrophic coastal system

Marine sponges are key players in the transfer of carbon from the pelagic microbial food web into the benthos. Selective uptake of prokaryotic picoplankton (<2 μm) by a demosponge (Callyspongia sp.), and carbon flux through this process, were examined for the first time in the oligotrophic coastal waters of southwestern Australia, where sponge abundance and biodiversity ranks among the highest in the world. Water sampling and flow rate measurements were conducted over five sampling occasions following the InEx method of Yahel et al. (2005), with heterotrophic bacteria and autotrophic Synechococcus cyanobacteria identified and enumerated by flow cytometry. Callyspongia sp. demonstrated high filtration efficiencies, particularly for high DNA (HDNA) bacteria (up to 85.3% in summer 2008) and Synechococcus (up to 91.1% in autumn 2007), however efficiency varied non-uniformly with time and food type (p < 0.01). Overall filtration efficiency for Synechococcus (86.6 ± 6.3%; mean ± s.d.) was always significantly higher (p < 0.05) than for low DNA (LDNA) bacteria (40 ± 17.2%), except during winter 2007 (p = 0.14) when ambient Synechococcus concentrations were lowest. When compared to ambient abundances of the different food types, Callyspongia sp. exhibited consistently negative selectivity for LDNA bacteria and positive selectivity for Synechococcus, while HDNA bacteria was generally a neutral or positive selection. The total carbon removal rate (sum of all prokaryotic picoplankton cells), calculated on a per unit area basis, varied significantly with time (p < 0.01), with lowest rates recorded during the winter (0.5 ± 0.4–0.6 ± 0.8 mg C m⁻² d⁻¹) and highest values recorded in summer (3.5 ± 1.9 mg C m⁻² d⁻¹). These flux estimates quantify the role of a demosponge species in the ultimate fate of prokaryotic picoplankton within the nearshore food webs of southwestern Australia, and support the conclusion that sponges actively select food particles that optimise their nutritional intake.     

Time-series measurements of macrobenthos abundance and sediment bioturbation intensity on a flood-dominated shelf

Four 70-m stations on the continental shelf offshore from the Eel River (northern California) were occupied at roughly four-month intervals between February 1995 and March 1998, and in August 1999. At each of the stations, profiles of excess ²³⁴Th were used to quantify sediment bioturbation intensity. In addition, at two of the stations macrofaunal abundance, species composition and functional groupings were quantified. During the study period, the Eel River displayed a range of hydrological conditions, with historically significant floods in January 1995 and January 1997 (return periods of 15 and 40 y, respectively), relatively low flows during the winters of 1995-1996 and 1998-1999 and an El Niño year characterized by moderate, but frequent discharges in 1997-1998. The January 1995 and 1997 floods deposited 3-7 cm of fine-grained, high porosity sediment with high C/N ratios and a terrestrial organic carbon signature at the study sites. The following general questions are addressed herein: (1) how do macrofaunal abundance, species composition and functional groupings vary over time? (2) Does the sediment deposition following the January 1997 flood constitute a major disturbance to the Eel shelf macrobenthos? (3) How does sediment bioturbation intensity vary in time/space and what are the main factors controlling this variation?The Eel shelf macrofauna is strongly dominated by subsurface-deposit feeding polychaetes, with anomalously low abundances of surface-deposit feeders and virtually no suspension feeders among the community dominants. The abundance data revealed a clear seasonal pattern, with peak density (∼4.5 × 10⁴ m⁻²) in the fall and a factor of two lower density in the late winter/spring (∼2 × 10⁴ m⁻²). Within this seasonal context there was little evidence for extraordinary mortality caused by the January 1997 flood, in that overall wintertime mortality and the mortality of most community dominants during a year (1995-1996) when there was no flood deposition were comparable to the mortality observed following the January 1997 flood. In contrast, the depth distribution of the macrofauna revealed a distinctive post-flood pattern, whereby a majority (55-70%) of individuals were temporarily found at depths >4 cm. This pattern suggests an active response by the resident fauna to sediment deposition, and supports the idea that the floods did not cause a widespread disturbance. Although there may not be clear evidence for short-term flood effects, the overall species composition and functional groupings do imply that the sedimentary environment (high sediment accumulation rates and abundant terrestrial organic matter) has had a long-term influence on the Eel shelf macrofauna.Model fits to ∼75 profiles of excess ²³⁴Th show that in general the data are consistent with a steady-state, biodiffusive model. The resultant mixing intensities ranged from 3 to 325 cm² y⁻¹, with averages (±standard deviation) of 35 ± 33, 24 ± 19, 37 ± 35, and22 ± 9 cm² y⁻¹ at stations C70, I70, L70, and O70, respectively. The average biodiffusivity for all stations and times was 29 ± 25 cm² y⁻¹ (N = 62). Due to the large amount of variability, which is consistent with other continental margin studies, it was not possible to detect significant spatial or temporal variability, although there is a hint of higher mixing intensities during the late summer - early fall, the period of maximal carbon flux to the seabed. Correlations between total macrofaunal abundance and mixing intensity are notably poor, whereas a slightly better correlation (r² = 0.22) was obtained between the abundance of large animals and bioturbation intensity. By explicitly considering organic carbon flux, or some measure of seabed food resources, and the abundance of larger organisms it may be possible to predict bioturbation intensity better in future studies, although the pervasive small-scale variability detected on the Eel River shelf warrants in-depth theoretical and experimental consideration.     

The planktonic food web of the Bizerte lagoon (south-western Mediterranean) during summer: I. Spatial distribution under different anthropogenic pressures

The structure and the trophic interactions of the planktonic food web were investigated during summer 2004 in a coastal lagoon of south-western Mediterranean Sea. Biomasses of planktonic components as well as bacterial and phytoplankton production and grazing by microzooplankton were quantified at four stations (MA, MB, MJ and R) inside the lagoon. Station MA was impacted by urban discharge, station MB was influenced by industrial activity, station MJ was located in a shellfish farming sector, while station R represented the lagoon central area. Biomasses and production rates of bacteria (7–33 mg C m⁻³; 17.5–35 mg C m⁻³ d⁻¹) and phytoplankton (80–299 mg C m⁻³; 34–210 mg C m⁻³ d⁻¹) showed high values at station MJ, where substantial concentrations of nutrients (NO₃⁻ and Si(OH)₄) were found. Microphytoplankton, which dominated the total algal biomass and production (>82%), were characterized by the proliferation of several chain-forming diatoms. Microzooplankton was mainly composed of dinoflagellates (Torodinium, Protoperidinium and Dinophysis) and aloricate (Lohmaniellea and Strombidium) and tintinnid (Tintinnopsis, Tintinnus, Favella and Eutintinnus) ciliates. Higher biomass of these protozoa (359 mg C m⁻³) was observed at station MB, where large tintinnids were encountered. Mesozooplankton mainly represented by Calanoida (Acartia, Temora, Calanus, Eucalanus, Paracalanus and Centropages) and Cyclopoida (Oithona) copepods, exhibited higher and lower biomasses at stations MA/MJ and MB, respectively. Bacterivory represented only 35% of bacterial production at stations MB and R, but higher fractions (65–70%) were observed at stations MA and MJ. Small heterotrophic flagellates and aloricate ciliates seemed to be the main controllers of bacteria. Pico- and nanophytoplankton represented a significant alternative carbon pool for micrograzers, which grazing represented 67–90% of pico- and nano-algal production in all stations. Microzooplankton has, however, a relatively low impact on microphytoplankton, as ≤45% of microalgal production was consumed in all stations. This implies that an important fraction of diatom production would be channelled by herbivorous meso-grazers to higher consumers at stations MA and MJ where copepods were numerous. Most of the microalgal production would, however, sink particularly at station MB where copepods were scare. These different trophic interactions suggest different food web structures between stations. A multivorous food web seemed to prevail in stations MJ and MA, whereas microbial web was dominant in the other stations.     

The role of two sediment-dwelling invertebrates on the mercury transfer from sediments to the estuarine trophic web

The annual total and organic mercury bioaccumulation pattern of Scrobicularia plana and Hediste diversicolor was assessed to evaluate the potential mercury transfer from contaminated sediments to estuarine food webs. S. plana was found to accumulate more total and organic mercury than H. diversicolor, up to 0.79 mg kg⁻¹ and 0.15 mg kg⁻¹ (wet weight) respectively, with a maximum annual uptake of 0.21 mg kg⁻¹ y⁻¹, while for methylmercury the annual accumulation was similar between species and never exceeded 0.045 mg kg⁻¹ y⁻¹. The higher organic mercury fraction in H. diversicolor is related to the omnivorous diet of this species. Both species increase methylmercury exposure by burrowing activities and uptake in anoxic, methylmercury rich sediment layers. Integration with the annual biological production of each species revealed mercury incorporation rates that reached 28 μg m⁻² y⁻¹, and to extract as much as 11.5 g Hg y⁻¹ (of which 95% associated with S. plana) in the 0.4 km² of the most contaminated area, that can be transferred to higher trophic levels. S. plana is therefore an essential vector in the mercury biomagnification processes, through uptake from contaminated sediments and, by predation, to transfer it to economically important and exploited estuarine species.     

A temporal analysis on the dynamics of deep-sea macrofauna: Influence of environmental variability off Catalonia coasts (western Mediterranean)

A seasonal analysis of deep-sea infauna (macrobenthos) based on quantitative sampling was conducted over the Catalan Sea slope, within the Besòs canyon (at ∼550-600 m) and on its adjacent slope (at 800 m). Both sites were sampled in February, April, June-July and October 2007. Environmental variables influencing faunal distribution were also recorded in the sediment and sediment/water interface. Dynamics of macrobenthos at the two stations showed differences in biomass/abundance patterns and trophic structures. Biomass was higher inside the Besòs canyon than on the adjacent slope. The community was mostly dominated by surface-deposit feeding polychaetes (Ampharetidae, Paraonidae, Flabelligeridae) and crustaceans (amphipods such as Carangoliopsis spinulosa and Harpinia spp.) inside the canyon, while subsurface deposit feeders (mainly the sipunculan Onchnesoma steenstrupii) were dominant over the adjacent slope. The taxonomic composition in the suprabenthic assemblages of polychaetes, collected on the adjacent slope by a suprabenthic sledge, was clearly different from that collected by the box-corer. The suprabenthic assemblage was dominated by carnivorous forms (mainly Harmothoe sp. and Nephthys spp.) and linked to higher near-bottom turbidity. Inside Besòs a clear temporal succession of species was related to both food availability and quality and the proliferation of opportunistic species was consistent with higher variability in food sources (TOC, C/N, ??¹³C) in comparison to adjacent slope. This was likely caused by a greater influence of terrigenous inputs from river discharges. Inside the canyon, Capitellidae, Spionidae and Flabelligeridae, in general considered as deposit feeders, were more abundant in June-July coinciding with a clear signal of terrigenous carbon (depleted ??¹³C, high C/N) in the sediments. By contrast, during October and under conditions of high water turbidity and increases of TOM, carnivorous polychaetes (Glyceridae, Onuphidae) increased. Total macrobenthos biomass found over Catalonian slopes were higher than that found in the neighboring Toulon canyon, probably because the two canyons are influenced by different river inputs, connected with distinct terrigenous sources.     

Annual Primary Production in Narragansett Bay with no Bay-Wide Winter–Spring Phytoplankton Bloom

Primary production was estimated over the annual cycle from ¹⁴C incubations conducted in 5 m deep enclosures and modeled for 16 stations in Narragansett Bay with data from biweekly surveys in which light, chlorophyll, attenuation coefficients and other parameters were measured. Annual values ranged from 160 g C m⁻² y⁻¹ in the lower West Passage to 619 g C m⁻² y⁻¹ at the mouth of the Providence River. The annual bay-wide, area mean fell near the middle of this range at 323 g C m⁻² y⁻¹ and was not apparently different from previous surveys. In the 1998 warm, El Niño winter, no bay-wide winter–spring phytoplankton flowered. Bloom limitation was correlated with warm temperatures which may have stimulated grazing rates. The lack of a bloom did not change annual levels of primary production but this alteration in carbon flow may impact macrofauna in the benthic infauna community.     

The fate of production in the central Arctic Ocean - top-down regulation by zooplankton expatriates?

We estimated primary and bacterial production, mineral nutrients, suspended chlorophyll a (Chl), particulate organic carbon (POC) and nitrogen (PON), abundance of planktonic organisms, mesozooplankton fecal pellet production, and the vertical flux of organic particles of the central Arctic Ocean (Amundsen basin, 89-88° N) during a 3 week quasi-Lagrangian ice drift experiment at the peak of the productive season (August 2001). A visual estimate of ≈15% ice-free surface, plus numerous melt ponds on ice sheets, supported a planktonic particulate primary production of 50-150 mg C m⁻² d⁻¹ (mean 93 mg C m⁻² d⁻¹, n = 7), mostly confined to the upper 10 m of the nutrient replete water column. The surface mixed layer was separated from the rest of the water column by a strong halocline at 20 m depth. Phototrophic biomass was low, generally 0.03-0.3 mg Chl m⁻³ in the upper 20 m and <0.02 mg Chl m⁻³ below, dominated by various flagellates, dinoflagellates and diatoms. Bacterial abundance (typically 3.7-5.3 × 10⁵, mean 4.1 × 10⁵ cells ml⁻¹ in the upper 20 m and 1.3-3.7 × 10⁵, mean 1.9 × 10⁵ cells ml⁻¹ below) and Chl concentrations were closely correlated (r = 0.75). Mineral nutrients (3 μmol NO₃ l⁻¹, 0.45 μmol PO₄ l⁻¹, 4-5 μmol SiO₄ l⁻¹) were probably not limiting the primary production in the upper layer. Suspended POC concentration was ∼30-105 (mean 53) mg C m⁻³ and PON ∼5.4-14.9 (mean 8.2) mg N m⁻³ with no clear vertical trend. The vertical flux of POC in the upper 30-100 m water column was ∼37-92 (mean 55) mg C m⁻² d⁻¹ without clear decrease with depth, and was quite similar at the six investigated stations. The mesozooplankton biomass (≈2 g DW m⁻², mostly in the upper 50 m water column) was dominated by adult females of the large calanoid copepods Calanus hyperboreus and Calanus glacialis (≈1.6 g DW m⁻²). The grazing of these copepods (estimated via fecal pellet production rates) was ≈15 mg C m⁻² d⁻¹, being on the order of 3% and 20% of the expected food-saturated ingestion rates of C. hyperboreus and C. glacialis, respectively. The stage structure of these copepods, dominated by adult females, and their unsatisfied grazing capacity during peak productive period suggest allochthonous origin of these species from productive shelf areas, supported by their long life span and the prevailing surface currents in the Arctic Ocean. We propose that the grazing capacity of the expatriated mesozooplankton population would match the potential seasonal increase of primary production in the future decreased ice perspective, diminishing the likelihood of algal blooms.     

Community structure and nutrition of deep methane-seep macrobenthos from the North Pacific (Aleutian) Margin and the Gulf of Mexico (Florida Escarpment)

Methane seeps occur at depths extending to over 7000 m along the world's continental margins, but there is little information about the infaunal communities inhabiting sediments of seeps deeper than 3000 m. Biological sampling was carried out off Unimak Island (3200–3300 m) and Kodiak Island (4500 m) on the Aleutian margin, Pacific Ocean and along the Florida Escarpment (3300 m) in the Gulf of Mexico to investigate the community structure and nutrition of macrofauna at these sites. We addressed whether there are characteristic infaunal communities common to the deep‐water seeps or to the specific habitats (clam beds, pogonophoran fields, and microbial mats) studied here, and ask how these differ from background communities or from shallow‐seep settings sampled previously. We also investigated, using stable isotopic signatures, the utilization of chemosynthetically fixed and methane‐derived organic matter by macrofauna from different regions and habitats. Within seep sites, macrofaunal densities were the greatest in the Florida microbial mats (20,961 ± 11,618 ind·m⁻²), the lowest in the Florida pogonophoran fields (926 ± 132 ind·m⁻²), and intermediate in the Unimak and Kodiak seep habitats. Seep macrofaunal densities differed from those in nearby non‐seep sediments only in Florida mat habitats, where a single, abundant species of hesionid polychaete comprised 70% of the macrofauna. Annelids were the dominant taxon (>60%) at all sites and habitats except in Florida background sediments (33%) and Unimak pogonophoran fields (27%). Macrofaunal diversity (H′) was lower at the Florida than the Alaska seeps, with a trend toward reduced richness in clam bed relative to pogonophoran field or non‐seep sediments. Community composition differences between seep and non‐seep sediments were evident in each region except for the Unimak margin, but pogonophoran and clam bed macrofaunal communities did not differ from one another in Alaska. Seep δ¹³C and δ¹⁵N signatures were lighter for seep than non‐seep macrofauna in all regions, indicating use of chemosynthetically derived carbon. The lightest δ¹³C values (average of species’ means) were observed at the Florida escarpment (−42.8‰). We estimated that on average animal tissues had up to 55% methane‐derived carbon in Florida mats, 31–44% in Florida clam beds and Kodiak clam beds and pogonophoran fields, and 9–23% in Unimak seep habitats. However, some taxa such as hesionid and capitellid polychaetes exhibited tremendous intraspecific δ¹³C variation (>30‰) between patch types. Overall we found few characteristic communities or features common to the three deep‐water seeps (>3000 m), but common properties across habitats (mat, clam bed, pogonophorans), independent of location or water depth. In general, macrofaunal densities were lower (except at Florida microbial mats), community structure was similar, and reliance on chemosynthesis was greater than observed in shallower seeps off California and Oregon.     

Microphytobenthic primary production as C uptake in sublittoral sediments of the Gulf of Trieste (northern Adriatic Sea): Methodological aspects and data analyses

From January 2003 to December 2004 microphytobenthic primary production was estimated both from in situ (MPPs) and in the laboratory (MPPp) ¹⁴C-incubation of slurries collected in a coastal site of the Gulf of Trieste (northern Adriatic Sea). MPPs values varied from −7.54 ± 3.12 to 34.59 ± 7.66 mg C m⁻² h⁻¹ over the whole period. The lowest MPPs were observed in November 2003 and August 2004, while the highest MPPs in July 2003 and May 2004, in correspondence with high PAR at the bottom. Significant correlations between MPPs and the microphytobenthic biomass (BIOM) (r = 0.75, p < 0.001), between MPPs and PAR at the bottom (r = 0.54, p < 0.01) and between MPPs and OXY (r = 0.50, p < 0.05) were revealed. MPPp values were higher than MPPs ones in 15 out of 23 observations, with the highest MPPp recorded in July 2003. At 17 m depth a seasonal pattern of sampling months was revealed by the cluster analysis. The role of abiotic parameters in determining this seasonal pattern was highlighted by the PCA, with the first axis correlated with MPPs and PAR, and the second one with temperature. Applying the fuzzy sets it resulted that spring months showed a higher degree of membership with MPPs, summer months with temperature and autumn–winter months with OXY. The microphytobenthic community did not seem to be photosynthetically active throughout the study period. From August–September to December low or negative MPPs values were recorded. We infer that during these months a shift from the autotrophic to heterotrophic metabolism of the benthic microalgae occurred in correspondence with low PAR and/or high temperature at the bottom. Despite the progressive lowering of the trophy of the study area occurred during the last 20 years, we found higher primary production values than those estimated two decades earlier.     

A preliminary investigation of the fish food web in the Gironde estuary,France, using dietary and stable isotope analyses

Carbon and Nitrogen stable isotopes and stomach contents analyses were used to investigate an estuarine fish food web and identify the contribution of these two methods to the knowledge and understanding of the food web's structure and its functioning. The nine most abundant fish species during the warm period in the Gironde estuary (southwest France, Europe) are examined. Observation of the stomach contents reflects a variety of feeding modes between fish species that consume a diverse assortment of prey, with limited dietary overlap. Nevertheless, when regarding the whole fish community, few prey species dominate the stomach contents. Nitrogen isotope ratios indicate a high intraspecific variability inducing an interspecific covering of the signatures. However, a tendency to δ¹⁵N enrichment according to the trophic position of the species studied was observed. Fish assemblages show a trend towards enrichment of their carbon isotopic signatures from the upper estuary (−20.8 ± 1.8‰) towards the lower estuary (−18.3 ± 1.6‰). But whatever the capture zone considered, most of the individual δ¹³C values for each fish analysed are comprised between −22 and −16‰. Only few specimens, belonging to migratory amphihaline species, have significantly lighter values.     

Effects of upwelling,tides and biological processes on the inorganic carbon system of a coastal lagoon in Baja California

The role of coastal lagoons and estuaries as sources or sinks of inorganic carbon in upwelling areas has not been fully understood. During the months of May–July, 2005, we studied the dissolved inorganic carbon system in a coastal lagoon of northwestern Mexico during the strongest period of upwelling events. Along the bay, different scenarios were observed for the distributions of pH, dissolved inorganic carbon (DIC) and apparent oxygen utilization (AOU) as a result of different combinations of upwelling intensity and tidal amplitude. DIC concentrations in the outer part of the bay were controlled by mixing processes. At the inner part of the bay DIC was as low as 1800 μmol kg⁻¹, most likely due to high water residence times and seagrass CO₂ uptake. It is estimated that 85% of San Quintín Bay, at the oceanic end, acted as a source of CO₂ to the atmosphere due to the inflow of CO₂-rich upwelled waters from the neighboring ocean with high positive fluxes higher than 30 mmol C m⁻² d⁻¹. In contrast, there was a net uptake of CO₂ and HCO₃⁻ by the seagrass bed Zostera marina in the inner part of the bay, so the pCO₂ in this zone was below the equilibrium value and slightly negative CO₂ fluxes of −6 mmol C m⁻² d⁻¹. Our positive NEP and ΔDIC values indicate that Bahía San Quintín was a net autotrophic system during the upwelling season during 2005.     

Particulate suspended matter concentrations in the Bahía Blanca Estuary,Argentina: Implication for the development of phytoplankton blooms

The inner zone of the Bahía Blanca Estuary is shallow, nutrient-rich and turbid. Tidal energy and water turbulence strongly affect the water column resulting in a well-mixed structure and high concentrations of suspended sediment. The phytoplankton community is mostly dominated by diatoms and the annual pattern has been characterized by a recurrent winter-early spring bloom. Here, we investigated to what extent the temporal variations of suspended particulate matter (SPM) regulate the phytoplankton blooms in the head of the estuary by light-limitation. Sampling was done on a fortnightly basis (weekly during the blooming season) at a fixed station in the inner zone of the estuary from January 2007 to February 2008. SPM concentrations and light extinction coefficients (k) in the water column were significantly correlated and showed relatively lower values during the phytoplankton maximal biomass levels. During winter, SPM and k reached values of 23.6 mg l⁻¹ and 0.17 m⁻¹ which were significantly lower than the annual means of 77.6 mg l⁻¹ and 2.94 m⁻¹, respectively. The particulate organic matter (POM) concentration was significantly correlated with the calculated phytoplankton biomass although the contribution of the latter to the total POM was rather low. Both, POM and biomass, had maximal values during winter (21.8 mg l⁻¹ and 393.5 μg C l⁻¹) and mid summer (24.3 mg l⁻¹ and 407.0 μg C l⁻¹), with cell densities up to 8 × 10⁶ cells l⁻¹ and chlorophyll a up to 24.6 μg l⁻¹. Our results suggest that the decrease of SPM concentrations in the water column with a concomitant increase in the penetration of solar radiation seems to be one of the main causes for the development of the phytoplankton winter bloom in the Bahía Blanca Estuary.     

渤海大型底栖动物种类组成与群落结构研究

本文利用2008年8月在渤海23个站位采集的样品对大型底栖动物的丰度、生物量、群落结构及其与环境因子的关系进行了研究。共鉴定出大型底栖动物300种,主要类群包括环节动物多毛类、节肢动物甲壳类、软体动物、棘皮动物等。研究海域大型底栖动物的平均丰度为1 094.7ind/m2,平均生物量为11.78g/m2,其中丰度最高区位于辽东湾,最低区位于渤海湾;生物量最高区位于渤海中部,最低区出现在渤海海峡湾口处。依据丰度和生物量进行了CLUSTER聚类和MDS标序图分析,结果可将研究站位划分为6个站组,均与沉积物底质类型密切相关。所研究海域底质类型包括黏土质粉砂、砂、砂质粉砂、粉砂质砂、砂-粉砂-黏土5种。通过BIOENV分析,发现影响大型底栖动物群落结构和分布的主要环境因子有水深、黏土粉砂含量、脱镁叶绿酸含量、叶绿素含量等。与20世纪90年代历史数据的对比发现,本次研究大型底栖动物的物种数目明显减少,平均丰度也减少,特别是优势物种表现出了小型化趋势,传统的大型种类,如心形海胆、双壳类等,被小个体的多毛类和甲壳类所取代。本研究表明渤海近年来受人类活动影响,出现了不同程度的污染,导致大型底栖动物群落结构发生了变化。     

Distribution of Deep-Sea Benthos in the Proposed Mining Area of Central Indian Basin

Abstract

During the first stage of the project work on the Indian Deep-Sea Environment Experiment (INDEX), the abundance and distribution of deep-sea benthos were surveyed in the Central Indian Basin for the collection of baseline data. The deep-sea community of the sediment was characterized by a moderately high standing crop and diverse fauna. The macrofaunal component was dominated by polychaetes (100% prevalence) and peracarid crustaceans, whereas the meiofauna was dominated by nematodes and harpacticoid cope-pods. The results of this study conform to the general distribution reported elsewhere. The macrofaunal abundance showed an inverse relation to the abundance of polymetallic nodules. However, the relation between meiofaunal vertical distribution and the vertical profile of the total organic matter and total labile matter was positive.     

High-resolution observations of aggregate flux during a sub-polar North Atlantic spring bloom

An aggregate flux event was observed by ship and by four underwater gliders during the 2008 sub-polar North Atlantic spring bloom experiment (NAB08). At the height of the diatom bloom, aggregates were observed as spikes in measurements of both particulate backscattering coefficient (b_bp) and chlorophyll a fluorescence. Optical sensors on the ship and gliders were cross-calibrated through a series of simultaneous profiles, and b_bp was converted to particulate organic carbon. The aggregates sank as a discrete pulse, with an average sinking rate of ∼75 m d⁻¹; 65% of aggregate backscattering and 90% of chlorophyll fluorescence content was lost between 100 m and 900 m. Mean aggregate organic carbon flux at 100 m in mid-May was estimated at 514 mg C m⁻² d⁻¹, consistent with independent flux estimates. The use of optical spikes observed from gliders provides unprecedented coupled vertical and temporal resolution measurements of an aggregate flux event.     

Influence of allochthonous organic matter on bacterioplankton biomass and activity in a eutrophic,sub-tropical estuary

Heterotrophic bacterial and phytoplankton biomass, production, specific growth rates, and growth efficiencies were studied in the Northern region of the Cananéia–Iguape estuarine system, which has recently experienced an intense eutrophication due to anthropogenic causes. Two surveys were carried out during spring and neap tide periods of the dry season of 2005 and the rainy season of 2006. This region receives large freshwater inputs with organic seston and phosphate concentrations that reach as high as 1.0 mg l⁻¹ and 20.0 μM, respectively. Strong decreasing gradients of seston and dissolved inorganic nutrients were observed from the river/estuary boundary to the estuary/coastal interface. Gradients were also observed in phytoplankton and bacterial production rates. The production rates of phytoplankton were 5.6-fold higher (mean 8.5 μg C l⁻¹ h⁻¹) during the dry season. Primary production rates (PP) positively correlated with salinity and euphotic depth, indicating that phytoplankton productivity was light-limited. On the other hand, bacterial biomass (BB) and production rates (BP) were 1.9- and 3.7-fold higher, respectively, during the rainy season, with mean values of up to 40.4 μg C l⁻¹ and 7.9 μg C l⁻¹ h⁻¹, respectively. Despite such a high BP, bacterial abundance remained <2 × 10⁶ cells ml⁻¹, indicating that bacterial production and removal were coupled. Mean specific growth rates ranged between 0.9 and 5.5 d⁻¹. BP was inversely correlated with salinity and positively correlated with temperature, organic matter, exopolymer particles, and particulate-attached bacteria; this last accounted for as much as 89.6% of the total abundance. During the rainy season, BP was generally much higher than PP, and values of BP/PP > 20 were registered during high freshwater input, suggesting that under these conditions, bacterial activity was predominantly supported by allochthonous inputs of organic carbon. In addition, BB probably represented the main pathway for the synthesis of high-quality (low C:N) biomass that may have been available to the heterotrophic components of the plankton food web, particularly nanoheterotrophs.     

The cycling and oxidation pathways of organic carbon in a shallow estuary along the Texas Gulf Coast

The cycling and oxidation pathways of organic carbon were investigated at a single shallow water estuarine site in Trinity Bay, Texas, the uppermost lobe of Galveston Bay, during November 2000. Radio-isotopes were used to estimate sediment mixing and accumulation rates, and benthic chamber and pore water measurements were used to determine sediment-water exchange fluxes of oxygen, nutrients and metals, and infer carbon oxidation rates. Using ⁷Be and ²³⁴Th_XS, the sediment-mixing coefficient (D_b) was 4.3 ± 1.8 cm² y⁻¹, a value that lies at the lower limit for marine environments, indicating that mixing was not important in these sediments at this time. Sediment accumulation rates (S_a), estimated using ¹³⁷Cs and ²¹⁰Pb_XS, were 0.16 ± 0.02 g cm⁻² y⁻¹. The supply rate of organic carbon to the sediment-water interface was 30 ± 3.9 mmol C m⁻² d⁻¹, of which ∼10% or 2.9 ± 0.44 mmol C m⁻² d⁻¹was lost from the system through burial below the 1-cm thick surface mixed layer. Measured fluxes of O₂ were 26 ± 3.8 mmol m⁻² d⁻¹ and equated to a carbon oxidation rate of 20 ± 3.3 mmol C m⁻² d⁻¹, which is an upper limit due to the potential for oxidation of additional reduced species. Using organic carbon gradients in the surface mixed layer, carbon oxidation was estimated at 2.6 ± 1.1 mmol C m⁻² d⁻¹. Independent estimates made using pore water concentration gradients of ammonium and C:N stoichiometry, equaled 2.8 ± 0.46 mmol C m⁻² d⁻¹. The flux of DOC out of the sediments (DOC_efflux) was 5.6 ± 1.3 mmol C m⁻² d⁻¹. In general, while mass balance was achieved indicating the sediments were at steady state during this time, changes in environmental conditions within the bay and the surrounding area, mean this conclusion might not always hold. These results show that the majority of carbon oxidation occurred at the sediment-water interface, via O₂ reduction. This likely results from the high frequency of sediment resuspension events combined with the shallow sediment mixing zone, leaving anaerobic oxidants responsible for only ∼10–15% of the carbon oxidized in these sediments.     

Spatial variability of meiofaunal communities at areas of contrasting depth and productivity in the Aegean Sea (NE Mediterranean)

The abundance and community structure of metazoan meiofauna were studied in deep-sea sediments from the north and south Aegean Sea (Eastern Mediterranean) in summer 1997 and spring 1998. The two areas varied in their surface primary productivity with the northern area being more productive. Meiofaunal densities displayed strong spatial variability while no temporal changes were observed. Total metazoan density ranged from 128 to 1251 ind./10 cm², with significantly higher values in the north. Meiofaunal biomass ranged from 27 to 391 μgC/10 cm² with higher values also in the north. At all stations nematodes dominated the community, comprising on average more than 91% of the total abundance. On a broad scale, meiofaunal densities displayed a positive correlation with food availability (sediment-bound chloroplastic pigments, carbohydrates and lipids); carbon mineralization (an indicator of organic matter turnover) was significantly higher in the northern Aegean, providing evidence of high organic-matter input and intense benthic-pelagic coupling. The spatial structure of the nematode community indicated that the two areas were similar in terms of their dominant genera (Halalaimus, Acantholaimus, and Thalassomonhystera).