鄂尔多斯盆地多级次地下水流系统中硝酸盐分布特征及其成因

水资源短缺的鄂尔多斯盆地内地下水遭受硝酸盐(NO^-₃)污染等问题日益突出,识别盆地不同地下水流系统的NO^-₃分布规律及其成因,对地下水资源的合理利用与保护具有重要意义。选取鄂尔多斯盆地北部湖泊集中区白垩系地下水系统为研究对象,基于水化学和聚类-主成分分析划分地下水流系统级次,在此基础上对比分析不同级次地下水流系统中NO^-₃分布特征,综合水化学和环境同位素分析识别多级次地下水流系统中NO^-₃来源及其潜在过程。研究表明：研究区ρ(NO^-₃)超出地下水质量标准(GB/T 14848-2017)Ⅲ类水标准的地下水样品集中在局部-中间地下水流系统,其超标率达到28%;区域地下水流系统中ρ(NO^-₃)均值约为1 mg/L。研究区不同级次地下水流系统中ρ(NO^-₃)分布特征主要与人类活动影响程度有...     

Characterization of proteases from <Emphasis Type="Italic">Planomicrobium</Emphasis> sp. L-2 isolated from the gastrointestinal tract of <Emphasis Type="Italic">Octopus variabilis</Emphasis> (Sasaki)

A crude protease produced from Planomicrobium sp. L-2 is described, and its effectiveness as an additive in liquid detergent evaluated. We isolate the protease-producing Planomicrobium sp. L-2 from the gastrointestinal tract of Octopus variabilis. At least three caseinolytic protease clear bands were observed in zymogram analysis. The crude alkaline protease was highly tolerant of a pH range from 7.0 to 9.0, and temperatures to 50°C after incubation for 1 h. Proteolytic enzymes were stable towards three surfactants (5% Tween 80, 1% Triton X-100 and 0.05% SDS) and an oxidizing agent (1% hydrogen peroxide), in addition to being highly stable and compatible with popular commercial laundry powered detergent brands available in China. Our study demonstrates the potential these proteases have for development into novel classes of detergent additive. This study also suggests that the gastrointestinal tract of Octopus variabilis may be a rich source of commercially valuable strains of enzyme.     

氮肥施用量对冬小麦氮肥利用率及土壤剖面NO － / 3－N动态分布的影响

通过 6个氮水平小区试验 ,研究了不同氮肥用量对冬小麦氮肥利用率和土壤剖面 NO3 -- N动态分布的影响。结果表明 ,氮肥利用率有随施氮量的增加而递减的趋势 ;土壤剖面 NO3 -- N含量则有随氮肥施用量增加而增加的趋势 ,而在同一氮水平下 ,从土壤表层到深层 (10 0 cm) ,则有递减的趋势。在冬小麦生育期中 ,以开花期 80～ 10 0 cm土壤剖面累积的 NO3 -- N量最多 ,因此最有可能淋洗出根层 ,对地下水造成污染     

The selection of alkaline protease-producing yeasts fi＇om marine environments and evaluation of their bioactive peptide production

A total of 400 yeast strains from seawater, sediments, saltern mud, marine fish guts, and marine algae were obtained. The protease activity of the yeast cultures was estimated, after which four strains (HN3.11, N11b, YF04C and HN4.9) capable of secreting extracellular alkaline protease were isolated. The isolated strains were identified as Aureobasidium pullulans, Yarrowia lipolytica, Issatchenkia orientalis and Cryptococcus cf. aureus. The optimal pH of the protease activity produced by strains HN3.11, YF04C, and HN4.9 was 9.0, while that of the protease produced by strain N11b was 10.0. The optimal temperature for protease activity was 45°C for strains HN3.11, N11b, and YF04C, and 50°C for strain HN4.9. After digestion of shrimp (Penaeus vannamei) protein and spirulina (Arthospira platensis) protein with the four crude alkaline proteases, the filtrate from spirulina (Arthrospira platensis) powder digested by the crude alkaline protease of strain HN3.11 was found to have the highest antioxidant activity (61.4%) and the highest angiotensin I converting enzyme (ACE)-inhibitory activities (68.4%). The other filtrates had much lower antioxidant activity and ACE-inhibitory activities.     

Cold-adaptive alkaline protease from the psychrophilic Planomicrobium sp.547: enzyme characterization and gene cloning

A psychrophilic bacterium strain 547 producing cold-adaptive alkaline protease was isolated from the deep sea sediment of Prydz Bay, Antarctica. The organism was identified as a Planomicrobium species by 16S rRNA analysis. The optimal and highest growth temperatures for strain 547 were 15℃ and 30℃, respectively. The extracellular protease was purified by ammonium sulfate precipitation and DEAE cellulose-52 chromatography. The optimal temperature and pH for the activity of the purified enzyme were 35 ℃ and pH 9.0, respectively. The enzyme retained approximately 40% of its activity after 2 h of incubation at 50℃. The enzymatic activity was inhibited by 1 mmol/L phenylmethyl sulfonylfluoride (PMSF) and hydrochloride 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF), indicating that it was a serine protease. The presence of Ca2+ and Mn2+ increased the activity of the enzyme. The protease gene with a size of 1 269 bp was cloned from Planomicrobium sp. 547 using primers designed based on the conserved sequences of proteases in GenBank. The Planomicrobium sp. 547 protease contained a domain belonging to the peptidase S8 family, which has a length of 309 amino acid (AA) residues. The alignment and phylogenetic analysis of the AA sequence indicated that the protease belonged to the subtilisin family.     

罗非鱼鱼鳞胶制备降血压胶原肽的工艺及酶解物活性

以罗非鱼鱼鳞胶为原料,制备对血管紧张素转换酶(ACE)有抑制活性的降血压肽。以水解度(DH)及ACE抑制率为指标,筛选水解鱼鳞胶的最适酶种,并对其水解工艺条件进行优化;采用超滤技术对所制备的降血压肽进行分离纯化,比较不同组分的ACE抑制率,测定高ACE抑制率组分的氨基酸组成,并运用MALDI-TOF-MS法分析其分子质量分布。结果表明,复合蛋白酶是水解鱼鳞胶最适酶种,在50℃、pH7.5、料液质量体积比1∶5、酶与底物质量比(mE/mS)1.5%条件下,酶解6 h,酶解液的ACE抑制作用最强,其水解度DH 73%,ACE的抑制率76.15%。酶解液经超滤分离所获得的分子质量小于2 000 u的组分对ACE抑制率最高,达83.15%;该组分的氨基酸主要含有Gly和Pro,含量分别为22.04%和11.40%;该组分分子质量范围为1 300~2 000 u。     

Screening and characterization of the alkaline protease isolated from PLI-1, a strain of Brevibacillus sp. collected from Indonesia’s hot springs

A total of 69 strains of thermophilic bacteria were isolated from water, soil and sediment samples from three Indonesia’s hot spring areas (Pantai cermin, Kalianda and Banyu wedang) by using Minimal Synthetic Medium (MSM). The extreme thermophile Brevibacillus sp. PLI-1 was found to produce extracellular thermophilic alkaline protease with optimal activity at 70℃ and pH 8.0-9.0. The molecular weight of the protease was estimated to be around 56 kD by SDS-PAGE. The maximum activity of the protease was 26.54 U mL-1. The protease activity did not decrease after 30 min and still retained more than 70% of relative activity after 60 min at 70℃ and pH 8.0. The ion Mg2+ was found to promote protease activity at both low and high concentrations, whereas Cu2+ and Zn2+ could almost completely inhibit the activity. Divalent cation chelator EDTA inhibited the enzyme activity by 55.06% ± 0.27%, while the inhibition caused by PMSF, Leupeptin, Pepstain A and Benzamidine were 66.78% ± 3.25%, 52.37% ± 0.25%, 62.47% ± 2.96% and 50.99% ± 0.24%, respectively. Based on these observations, the enzyme activity was conspicuously sensitive to the serine and cysteine protease inhibitors. All these results indicated that the protease isolated from the strain PLI-1 was a thermophilic protease and had a high-temperature stability and a pH stability.     

复合酸化剂对鲤生长及消化酶活性的影响

在鲤鱼(Cyprinus carpio)基础饲料中,分别添加不同梯度的复合酸化剂(质量分数分别为0、0.2%、0.3%),研究复合酸化剂对其生长和消化酶活性的影响。结果表明:1)添加复合酸化剂显著(P<0.05)提高鲤鱼的相对增重率、特定生长率、饵料转化效率、饲料蛋白质效率以及饲料蛋白质沉积率等生长性能指标;2)添加质量分数0.3%的复合酸化剂时,鲤鱼肝胰脏、前肠和中肠的蛋白酶活性显著提高(P<0.05),酸化剂组的淀粉酶和脂肪酶活性有升高的趋势,但与对照组差异不显著;3)鱼体的粗蛋白质、粗脂肪、粗灰分、水分、肥满度及内脏比等品质指标在各组间差异不显著。综上所述,鲤鱼饲料中添加以柠檬酸、乳酸、磷酸等为主的复合酸化剂,可显著提高鲤鱼肝胰脏、前肠和中肠的蛋白酶活性,促进鲤鱼生长。     

Effects of seasonal variation on soil microbial community structure and enzyme activity in a Masson pine forest in Southwest China

Soil microbial communities and enzyme activities play key roles in soil ecosystems. Both are sensitive to changes in environmental factors,including seasonal temperature, precipitation variations and soil properties. To understand the interactive mechanisms of seasonal changes that affect soil microbial communities and enzyme activities in a subtropical masson pine(Pinus massoniana) forest, we investigated the soil microbial community structure and enzyme activities to identify the effect of seasonal changes on the soil microbial community for two years in Jinyun Mountain National Nature Reserve, Chongqing, China. The soil microbial community structure was investigated using phospholipid fatty acids(PLFAs). The results indicated that a total of 36 different PLFAs were identified, and 16:0 was found in the highest proportions in the four seasons, moreover, the total PLFAs abundance were highest in spring and lowest in winter. Bacteria and actinomycetes were the dominant types in the study area. Seasonal changes also had a significant(P 0.05) influence on the soil enzyme activity. The maximum and minimum values of the invertase and catalase activities were observed in autumn and winter, respectively. However, the maximum and minimum values of the urease and phosphatase acid enzymatic activities were found in spring and winter, respectively. Canonical correspondence analysis(CCA) analysis revealed that the seasonal shifts in soil community composition and enzyme activities were relatively more sensitive to soil moisture and temperature, but the microbial community structure and enzyme activity were not correlated with soil pH in the study region. This study highlights how the seasonal variations affect the microbial community and function(enzyme activity)to better understand and predict microbial responses to future climate regimes in subtropical area.     

Purification and characterization of an alkaline protease from <Emphasis Type="Italic">Micrococcus</Emphasis> sp. isolated from the South China Sea

Protease is wildly used in various fields, such as food, medicine, washing, leather, cosmetics and other industrial fields. In this study, an alkaline protease secreted by Micrococcus NH54PC02 isolated from the South China Sea was purified and characterized. The growth curve and enzyme activity curve indicated that the cell reached a maximum concentration at the 30th hour and the enzyme activity reached the maximum value at the 36th hour. The protease was purified with 3 steps involving ammonium sulfate precipitation, ion-exchange chromatography and hydrophobic chromatography with 8.22-fold increase in specific activity and 23.68% increase in the recovery. The molecular mass of the protease was estimated to be 25 kDa by SDS-PAGE analysis. The optimum temperature and pH for the protease activity were 50°C and pH 10.0, respectively. The protease showed a strong stability in a wide range of pH values ranging from 6.0–11.0, and maintained 90% enzyme activity in strong alkaline environment with pH 11.0. Inhibitor trials indicated that the protease might be serine protease. But it also possessed the characteristic of metalloprotease as it could be strongly inhibited by EDTA and strongly stimulated by Mn²⁺. Evaluation of matrix-assisted laser desorption ionization/time-of-flight MS (MALDI-TOF-TOF/MS) showed that the protease might belong to the peptidase S8 family.     

脂肪水平对厚唇弱棘鯻生长及肠道脂肪酶活性的影响

以鱼油为脂肪源,配制成脂肪水平(质量分数,下同)分别为3.1%、5.8%、9.4%、11.9%和15.1%的5种等氮饲料,探讨饲料脂肪水平对初始体重为(4.05±0.11)g的厚唇弱棘鯻(Hephaestus fuliginosus)生长及肠道脂肪酶活性的影响。结果表明,随着饲料脂肪含量提高,实验用鱼脏体比、肥满度及肌肉粗蛋白含量也随着提高,但各组间差异不显著(P>0.05)。饲料脂肪水平从3.1%增加到9.4%,厚唇弱棘鯻的饲料效率呈升高趋势,相对增重率值由76.42%±5.88%升至102.09%±5.31%;而当饲料脂肪水平从9.4%提高至15.1%,饲料效率呈降低趋势(P<0.05),相对增重率值降为70.54%±2.06%。随着饲料脂肪水平提高,厚唇弱棘鯻肝体比、肌肉粗脂肪含量、肠道脂肪酶活性均呈上升趋势,且各组间差异显著(P<0.05)。表明本实验条件下饲料脂肪含量超过9.4%可导致厚唇弱棘鯻生长和饲料利用下降,对其形体特征、肌肉粗脂肪含量以及肠道脂肪酶活性均有显著影响。     

CUMULATIVE EFFECTS OF DIFFERENT CULTIVATING PATTERNS ON PROPERTIES OF ALBIC SOIL IN SANJIANG PLAIN

1INTRODUCTION Albic soil is one of the main soils with low yield in the Sanjiang Plain, with an area of 200×103ha, accounting for 19% of the total area, among which 88.4×103ha is farmland, being 25.4% of the total area of farmland in this region. The albic soil occupies 44% of total area of farmland in the state-owned farms of Heilongjiang Province.Therearetworeasonsforthe lowyield ofalbic soil. Firstly, the upper layer of the soil is thin, only 10- 20cm for low yield field and 20- 25c…     

几种农作物矿物增效复合专用肥的应用研究

由沸石、膨润土和麦饭石等几种物化性能优越的非金属矿物按一定比例配合并粉碎成一定的粒度,再根据不同农作物生长所需的养分特点,与氮、磷或氮、磷、钾肥和微素（ 肥） 混合制成矿物增效复合专用肥。这种肥料既具有普通复合专用肥的特点,还具有保水、保肥和改良土壤之功能     

Labile organic matter content and distribution as affected by six-year soil amendments to eroded Chinese mollisols

Labile organic carbon （LOC） is a fraction of soil organic carbon （SOC） with rapid turnover time and is affected by soil fertilization. This investigation characterized the SOC content, LOC content and LOC distribution in the treatment plots of surface soil erosion at five levels （0-, 5-, 10-, 20- and 30-cm erosion）. The soil had received contrasting fertilizer treatments （i.e., chemical fertilizer or chemical fertilizer ＋ manure） for 6 years. This study demonstrated that both SOC and various LOC fractions contents were higher in the plots with fertilizer ＋ manure than in those with fertilizer alone under the same erosion conditions. The SOC and LOC contents de- creased as the erosion depth increased. Light fraction organic carbon, particulate organic carbon, easily oxidizable organic carbon （KMnO4-oxydizable organic carbon）, and microbial biomass carbon were 27% 57%, 37%-7%, 20%-25%, and 29%-33% higher respectively in the fertilizer ＋ manure plots, than in the fertilizer alone plots. Positive correlations （p 〈 0.05） between SOC content and different fractions contents were observed in all plots except the correlation between total SOC content and water-soluble organic carbon content in the different fertilization treatments. Obviously, fertilizer ＋ manure treatments would be conducive to the accumulation of LOC and SOC in the Black soil of Northeast China.     

Effects of long-term medieval agriculture on soil properties: A case study from the Kislovodsk basin,Northern Caucasus,Russia

The chemical properties and biological activities of soils were studied in the vicinity of the medieval settlement Podkumskoe-3 in the Kislovodsk basin (Northern Caucasus, Russia). Between the 5th and 8th centuries this area was ploughed regularly, but it was then abandoned up to the present day. It has been established that past human activity leads to soil undergoing significant transformations in terms of microbial communities and enzyme activity, and that such changes are maintained over long periods. Long-term manuring in the middle of the first millennium AD led to an increase in organic carbon content and the accumulation of nitrate nitrogen. Soils of ancient abandoned fields are associated with increases in microbial biomass, number of saprotrophic bacteria, urease activity, and fungal mycelium biomass. The observed changes in the microbiological and biochemical properties of soil were conditioned by secondary anthropogenically induced succession after the abandonment of arable lands.     

氮钾配施对生姜产量和砂姜黑土肥力的效应

研究了淮北平原砂姜黑土地区生姜氮钾配施的增产效应和对土壤养分的影响。结果表 明:氮钾配施可以大幅度提高生姜的产量,施钾的增产幅度,柴姜为15.3%～58.4%,平均增产率 为35.8%;狮子头姜产量提高18.2%～46.9%,平均增产28.7%。增施氮钾肥可有效提高土壤有 机质、全N、有效P、速效K、缓效K、有效Ca和有效Mg含量,降低NO3-盐含量,促使土壤pH趋于 中性,对全P和碱解N含量影响较小。土壤N素和P素大量盈余,不施钾时土壤K亏损严重,施钾 处理土壤钾基本上处于盈余状态,有利于维持和提高土壤肥力。     

土地质量地球化学评价数据库建库方法探讨——以章丘市刁镇和辛寨镇为例

土地质量地球化学评价数据库是以土壤地球化学调查为主,同时开展大气干(湿)沉降物、灌溉水、化肥及农作物等介质的地球化学调查数据和分析数据资料为基础,利用MapGIS软件管理编制成果图件;利用Access数据库管理规范成果数据;利用中国地调局发展研究中心下发的《土地质量地球化学调查与评价数据录入子系统》将数据资料数字化,从而建立评价数据库。数据库内容包括区域地理地质、地形地貌、土壤类型及土地利用等基础空间数据库;包括采样介质的工作区采样布置、单元素地球化学图、土壤元素分级图及大气环境、灌溉水环境、土壤质量、土地质量综合等级图为核心的空间评价数据库;包括土地规划图、特色农产品种植规划建议图、施肥建议图等各种成果图件。数据库实现了对样品及相应的众多的化验分析数据的科学有效的管理,便于数据的统一保存、分析和使用,为评价区土地质量地球化学调查评价和总体综合研究提供了基础资料,为促进农业经济规划调整,现代绿色农业发展和优化、特色土地资源开发与利用提供重要依据。     

Organic carbon losses by eroded sediments from sloping vegetable fields in South China

Soil Organic Carbon(SOC) is the most important component of soil. Though small, it determines soil fertility and prevents soil losses. In this study, we examined relationships between the Particle–Size Distribution(PSD) of the eroded sediment and SOC loss, and evaluated the effects of plant coverage ratios(0%, 15%, 30%, 45%, 60% and 90%), slope lengths(2 m, 4 m), fertilizer treatments(unfertilized control(CK), compound N–P–K fertilizer(CF), and organic fertilizer(OF)) on SOC loss and the SOC enrichment ratio(ERSOC) in the eroded sediments. The experimental results showed that longer slope length and lower surface cover ratios produced larger surface runoff and the eroded sediments, resulting in larger SOC losses. The average SOC loss was greatest in the OF treatment and SOC loss was mainly associated with the eroded sediment. Surface runoff, which causes soil erosion, is a selective transportation process, hence there were more claysized particles(2 μm) and silt-sized particles(2-50 μm) in the eroded sediments than in the original soils. SOC was enriched in the eroded sediments relative to in the original soil when ERSOC 1. ERSOC was positively correlated with ER_(clay)(2 μm)(R~2 = 0.68) and ERfine silt(2–20 μm)(R~2 = 0.63), and from all thesize particle categories of the original soil or the eroded sediments, more than 95% of SOC was concentrated in small-sized particles(50 μm). The distribution of SOC in different-sized particles of the original soil and the eroded sediment is primarily associated with clay-sized particles and fine silt-sized particles, thus we conclude that as the eroded sediment particles became finer, more SOC was absorbed, resulting in more severe SOC loss.     

Crop residue incorporation and nitrogen fertilizer effects on greenhouse gas emissions from a subtropical rice system in Southwest China

Crop residue incorporation has been widely accepted as a way to increase soil carbon (C) sequestration and sustain soil fertility in agroecosystems. However, effect of crop residue incorporation on greenhouse gas (GHG) emissions in rice paddy soils remains uncertain. A field experiment was conducted to quantify emissions of CH₄ and N₂O and soil heterotrophic respiration (R_H) from a paddy rice field under five different crop residue treatments (i.e., 150 kg N ha^-1 of synthetic N fertilizer application only [NF], 150 kg N ha^-1 of synthetic N fertilizer plus 5.3 Mg ha^-1 wheat residue [NF-WR1], 150 kg N ha^-1 of synthetic N fertilizer plus 10.6 Mg ha^-1 wheat residue [NF-WR2], 75 kg N ha^-1 of synthetic N fertilizer plus 10.6 Mg ha^-1 wheat residue [50%NF-WR2] and 150 kg N ha^-1 of synthetic N fertilizer plus 21.2 Mg ha^-1 wheat residue [NF-WR3]) in southwest China. Our results showed that crop residue incorporation treatments (NF-WR1, NF-WR2, 50%NF-WR2, NF-WR3) significantly increased CH₄ emissions by at least 60%, but N₂O emissions were not enhanced and even suppressed by 25% in the NF-WR3 treatment as compared to the NF treatment. Soil R_H emissions were comparable among experimental treatments, while crop residue incorporation treatments significantly increased soil carbon sequestrations relative to the NF treatment. Overall, CH₄ emissions dominated total global warming potentials (GWP) across all experimental treatments. The average yieldscaled GWPs for the NF and NF-WR1 treatments were significantly lower than for the NF-WR2, 50%NFWR2 and NF-WR3 treatments. Given the comparable yield-scaled GWPs between the NF and NF-WR1 treatments, the NF-WR1 treatment could gain net carbon sequestration as compared with the NF treatment with net soil carbon loss. Our findings suggest that the NF-WR1 treatment should be an effective option to sustain rice production while mitigating GHG emissions from the rice field in China.     

Effect of potassium on soil conservation and productivity of maize/cowpea based crop rotations in the north-west Indian Himalayas

Plots under conservation tillage may require higher amount of potassium (K) application for augmenting productivity due to its stratification in upper soil layers, thereby reducing K supplying capacity in a medium or long-term period. To test this hypothesis, a field experiment was performed in 2002-2003 and 2006-2007 to study the effect of K and several crop rotations on yield, water productivity, carbon sequestration, grain quality, soil K status and economic benefits derived in maize (Zea mays L)/cowpea (Vigna sinensis L.) based cropping system under minimum tillage (MT). All crops recorded higher grain yield with a higher dose of K (120 kg K₂O ha^-1) than recommended K (40 kg K₂O ha^-1). The five years’ average yield data showed that higher K application (120 kg K₂O ha^-1) produced 16.4% (P<0.05) more maize equivalent yield. Cowpea based rotation yielded 14.2% (P<0.05) higher production than maize based rotation. The maximum enhancement was found in cowpea-mustard rotation. Relationship between yield and sustainable indices revealed that only agronomic efficiency of fertilizer input was significantly correlated with yield. Similarly, higher doses of K application not only increased the water use efficiency (WUE) of all crops, but also reduced runoff and soil loss by 16.5% and 15.8% under maize and 23.3% and 19.7% under cowpea cover, respectively. This study also revealed that on an average 16.5% of left over carbon input contributed to soil organic carbon (SOC). Here, cowpea based rotation with the higher K application increased carbon sequestration in soil. Potassium fertilization also significantly improved the nutritional value of harvested grain by increasing the protein content for maize (by 9.5%) and cowpea (by 10.6%). The oil content in mustard increased by 5.0% and 6.0% after maize and cowpea, respectively. Net return also increased with the application of the higher K than recommended K and the trend was similar to yield. Hence, the present study demonstrated the potential yield and profit gains along with resource conservation in the Indian Himalayas due to annual additions of higher amount of K than the recommended dose. The impact of high K application was maximum in the cowpea-mustard rotation.