草鱼、团头鲂对伊乐藻的消化与利用

本文分析了伊乐藻的生化成分和能值,测定了草鱼、团头鲂对伊乐藻生化成分的消化率,草鱼对伊乐藻的最大摄食量、蛋白质利用率和饲料系数。结果表明,伊乐藻含水率为89.8％,干物质中粗蛋白质、粗脂肪、粗纤维素和无氮浸出物含量分别为23.52％、2.88％、26.71％和29.27％,含有17种常见氨基酸,总量为198.04mg/g(干重)。在平均水温20.4℃的室外条件下,草鱼,团头鲂对伊乐藻的干物质消化率分别为56.02％、54.97％,对粗蛋白质消化率分别为79.05％、74.96％,对氨基酸总量的消化率均为77.00％,对粗脂肪消化率分别为73.96％,64.63％,对碳水化合物消化率分别为58.46％、51.36％。在伊乐藻饲养试验中,草鱼每天最大摄食量(Y)与试验鱼体重(X)相关显著,相关系数为0.9154,关系式为Y=3.9449X~(0.8357)。在网箱中饲养草鱼,伊乐藻的粗蛋白质利用率为17.1％,饲料系数为48.2。在小型围栏中饲养草鱼,伊乐藻的粗蛋白质利用率为13.1％,饲料系数为63.4。     

Calcium isotope fractionation in coccoliths of cultured Calcidiscus leptoporus, Helicosphaera carteri, Syracosphaera pulchra and Umbilicosphaera foliosa

Four species of marine calcifying algae, the coccolithophores Calcidiscus leptoporus, Helicosphaera carteri, Syracosphaera pulchra and Umbilicosphaera foliosa were grown in laboratory cultures under temperatures varying between 14 and 23 °C, and one species, C. leptoporus, under varying [CO₃²⁻], ranging from 105 to 219 μmol/kg. Calcium isotope compositions of the coccoliths resemble in both absolute fractionation and temperature sensitivity previous calibrations of marine calcifying species e.g. Emiliania huxleyi (coccolithophores) and Orbulina universa (planktonic foraminifera) as well as inorganically precipitated CaCO₃, but also reveal small species specific differences. In contrast to inorganically precipitated calcite, but similar to E. huxleyi and O. universa, the carbonate ion concentration of the medium has no statistically significant influence on the Ca isotope fractionation of C. leptoporus coccoliths; however, combined data of E. huxleyi and C. leptoporus indicate that the observed trends might be related to changes of the calcite saturation state of the medium. Since coccoliths constitute a significant portion of the global oceanic CaCO₃ export production, the Ca isotope fractionation in these biogenic structures is important for defining the isotopic composition of the Ca sink of the ocean, one of the key parameters for modelling changes to the marine Ca budget over time. For the present ocean our results are in general agreement with the previously postulated and applied mean value of the oceanic Ca sink (Δ_sed) of about − 1.3‰, but the observed inter- and intra-species differences point to possible changes in Δ_sed through earth history, due to changing physico-chemical conditions of the ocean and shifts in floral and faunal assemblages.     

黑藻(Hydrilla verticillata)对铜绿微囊藻(Microcystis aeruginosa)抑制作用

采用黑藻(Hydrilla verticillata)和铜绿微囊藻(Microcystis aeruginosa)共同培养的实验方法,追踪测定微囊藻的生长量、光合速率等相关生理生化指标以及观察藻细胞超微结构变化,研究黑藻对铜绿微囊藻的抑制作用及其机制．结果表明,黑藻对铜绿微囊藻的生长有明显的抑制作用,表现为藻细胞生长量显著降低,细胞超微结构进行性损伤直至细胞解体,藻体叶绿素a含量和光合速率急剧下降,呼吸速率、超氧物歧化酶(SOD)活性均呈现先升高后下降的趋势,膜脂过氧化产物丙二醛(MDA)的积累量与膜的损伤程度相一致．在黑藻养殖水中初步分离得到黑藻分泌的抑藻物质．     

路南海菜花的核型分析及其海菜花属的演化探讨

海菜花属(Ottelia Pers.)为沉水单子叶植物,只生长于淡水生境中,具有重要的生态价值、环境指示作用以及植物系统学研究意义.本文首次报道了特产于我国云南石林湖泊中的路南海菜花(Ottelia acuminata var.lunanensis H.Li)的体细胞染色体数目为22条,核型属"1B"型,核型公式2n=2x=22=18m+4sm.结合已研究的花部特征和核型资料讨论海菜花属种间的亲缘关系及演化,为海菜花(Ottelia)的利用和保护提供细胞生物学证据.     

Effect of mixed toxic diets (Microcystis and Chlorella) on the rotifers Brachionus calyciflorus and Brachionus havanaensis cultured alone and together

Brachionus havanaensis is a common planktonic rotifer sometimes found together with Brachionus calyciflorus in Microcystis-infected waterbodies in México. Here, we evaluate the impact of mixed diets (dry weight basis, 0%, 25%, 50%, 75% and 100% Microcystis or Chlorella) on the growth of and competition between B. calyciflorus and B. havanaensis. In general, a higher proportion of Microcystis aeruginosa in the diet resulted in decreased population growth rate of both rotifer species. Whether grown separately or together, B. havanaensis was always numerically more abundant than B. calyciflorus, regardless of the proportion of Microcystis in the diet. However, when both species were grown together, the impact of M. aeruginosa in the diet had a more adverse effect on B. calyciflorus than on B. havanaensis. When grown alone, both rotifer species showed better population growth on a diet of 75% Chlorella and 25% Microcystis than either 100% Chlorella or Microcystis.     

水华微囊藻(Microcystis flos-aquae)生长周期中腐殖酸的释放特性

由于严重的水体富营养化,太湖长期暴发蓝藻水华,这些蓝藻在代谢及消亡过程中大量释放包括腐植酸在内的有机物,不仅影响供水水质,并且进一步加重了水华程度.以太湖水华优势藻种——水华微囊藻(Microcystis flos-aquae T34)为研究对象,对藻株生长周期中的藻细胞、浮游细菌进行计数,并使用TOC仪与三维荧光光谱对培养液中提取的腐殖酸进行定量与定性分析,探究水华微囊藻在生长过程中藻细胞密度、浮游细菌密度与腐殖酸浓度的变化规律.结果表明,培养液中腐殖酸浓度的变化趋势与细菌数量变化趋势一致,但与细菌数量曲线拐点相比,腐殖酸浓度曲线拐点出现两周的延迟.腐殖酸产量在藻细胞对数期较低,当水华微囊藻进入稳定期与衰亡期后,腐殖酸大量产生,其浓度迅速增加,最高可达到28.6 mg/L.根据三维荧光光谱分析,水华微囊藻所产生的腐殖酸特征峰出现在(235~245 nm)/(380~425 nm),属于类富里酸荧光峰.本研究初步探究蓝藻与水体腐殖酸之间的关系,为水体腐殖酸来源的研究提供了新的思路.     

铜绿微囊藻和四尾栅藻光合利用碳酸氢盐探讨

为了探讨羹类光合过程对碳酸氧盐的利用,研究不同pH值下铜绿微囊藻和四尾栅藻的净光合速率,并着重研究这两株藻的碳酸氢盐摄取速率.结果表明,从弱酸性到弱碱性,四尾栅藻的净光合速率都比铜绿微囊藻高.由pH值超过pH转折点后的无机碳摄取速率计算得到的碳酸氢盐摄取速率对铜绿微囊藻和四尾栅藻分别为142.14和314.08μmo1DIC/(g·h,FW),故这两株藻都可利用碳酸氢盐作为碳源进行光合,且均为强碳酸氧盐利用者;但是,四尾栅藻对碳酸氲盐的利用能力强于铜绿微囊藻.另外,与四尾栅藻相比,铜绿微囊藻的碳酸氧盐摄取速率更易受抑制剂的抑制.     

The empirical relationships between M s, m b and M L for China and vicinity

Data from 753 earthquakes are used to determine a relationship between surface-wave magnitude (M _s) and bodywave magnitude (m _b), and from 541 earthquakes to determine a relationship between surface-wave magnitude (M _s) and local magnitude (M _L) for China and vicinity: M _s=0.9883 m _b-0.0420, M _s=0.9919 M _L-0.1773. The relationship of M _s versus m _b is obtained for 292 events occurred in the Chinese mainland in the time period from 1964 to 1996, 291 events occurred in Taiwan in the time period from 1964 to 1995 and 170 events occurred in the surrounding area. Standard deviation of the fitting is 0.445. Relationship of M _s versus M _L is obtained for 36 events occurred in the Chinese mainland, 293 events occurred in Taiwan, China and 212 events occurred in the surrounding area. The total amount is 541 events. Standard deviation of the fitting is 0.4673. The uncertainties of the converted M _s in different magnitude intervals can be estimated using complementary cumulative distribution function (CCDF). In the relationship of M _s versus m _b, taking ±0.25 as a range of uncertainties, in magnitude interval m _b 4.0–4.9, the probabilities for the converted M _s taken value less than (M _s-0.25) and more than (M _s+0.25) are 17% and 27% respectively. Similarly, we have probabilities for m _b 5.0–5.9 are 34% and 20% and that for m _b 6.0–6.9 are 11% and 47%. In the relationship of M _s versus M _L, if the range of uncertainties is still taken as ±0.25, the corresponding probabilities for magnitude interval M _L 4.0–4.9 are 22% and 38%, for M _L 5.0–5.9 are 20% and 15% and for magnitude interval M _L 6.0–6.9, are 15% and 29%, respectively. The relationships developed in this paper can be used for the conversion of one magnitude scale into another magnitude scales conveniently. The estimation of uncertainties described in this paper is more accurate and more objective than the usual estimation expressed by deviation. The estimations described in this paper indicate various dispersions in different magnitude intervals of original data. The estimations of uncertainties described by probabilities can be well connected with the total estimations of uncertainties in seismic hazard assessment.     

河蚬(Corbicula fluminea)对霍甫水丝蚓(Limnodrilus hoffmeisteri)生物扰动的抑制效应

近年来太湖局部区域水体富营养化仍有加剧趋势,这可能与底栖动物生物扰动促进沉积物营养盐释放有关.耐污种霍甫水丝蚓(Limnodrilus hoffmeisteri)与常被用于生态修复的河蚬(Corbicula fluminea)均为太湖底栖动物优势种类,本文设计了双因素4组处理(对照组、河蚬组、霍甫水丝蚓组和混合组(霍甫水丝蚓和河蚬))的室外受控实验,研究在太湖2种种群分布交错区,河蚬能否对霍甫水丝蚓的生物扰动产生抑制效应.结果表明:霍甫水丝蚓扰动能显著增加水体总氮、总磷浓度,同时促进浮游植物生长;河蚬通过滤食和生物沉降作用显著降低水体总氮、总磷和悬浮物浓度,抑制浮游植物生长,从而促进了底栖藻类的生长;河蚬对霍甫水丝蚓生物扰动效应有抑制作用.     

同形溞(Daphnia similis)对幽蚊幼虫反捕食策略的模拟研究

枝角类溞会由于捕食者的存在而发生形态、行为、生活史等的变化.通过为期10 d对太湖春、夏季优势种同形溞(Daphnia similis)在有幽蚊幼虫(CL)、无幽蚊幼虫(CK)及培养过幽蚊幼虫的过滤水(FL)3个环境水平的模拟实验,发现同形溞在有幽蚊幼虫和培养过幽蚊幼虫的过滤水处理下平均体长、累积产仔数及平均产仔数均显著大于无幽蚊幼虫组,表明幽蚊幼虫释放的信息素能改变同形溞的体长、产仔数等生活史参数,这种改变会降低同形溞被捕食的风险.说明在自然环境下,幽蚊幼虫等无脊椎捕食者能通过直接捕食和信息素的间接作用共同影响枝角类种群.     

小球藻和铜绿微囊藻的高浓度Chl-a高光谱定量模型

本研究将地物高光谱遥感技术应用于室内实验,从而得到小球藻和铜绿微囊藻的高光谱特征.通过多种半经验方法,如单波段、波段比值和微分法,建立了两藻种最优的Chl-a高光谱定量模型,并与室外情况进行了对比.结果表明:小球藻的最优定量模型为Chl-a=174.6 1138292(R703) 2.3(109[(R703)]2(p＜0.01),相应的方法适宜性为:一阶微分法＞单波段法＞波段比值法;铜绿微囊藻的最优定量模型为Chl-a=5299164(R757)1.9773(p＜0.01),相应的方法适宜性为:单波段法＞波段比值法＞一阶微分法;从高光谱特征来看,小球藻在540 nm和700 nm附近存在明显的特征波峰,其位置随Chl-a浓度增大而向长波方向偏离,铜绿微囊藻在530 nm、660 nm和700 nm附近存在3个较强的特征波峰,在610 nm和680 nm附近存在明显的波谷;与以往室外研究不同的是铜绿微囊藻的反射率在400-500 nm之间的R值并不低,是因为没有非藻类颗粒物的影响,总吸收明显降低.     

水网藻附着对亚洲苦草光合特性的影响

运用水下饱和脉冲荧光仪(Diving-PAM)测定附着了水网藻的亚洲苦草(Vallisneria asiatica)叶片的荧光参数和快速光响应曲线.在水网藻主要附着的苦草叶片上部,15 d后Fv/Fm显著下降,光系统(PS)的光化学效率显著降低;附着水网藻的叶片通过热的形式耗散掉的能量极显著增加(P＜0.01),有效荧光产量、光化学淬灭系数和相对光合电子传递速率(rETR)、Chl.a、类胡萝卜素(Car)、Chl.a/Chl.b和Car/Chl.a均显著降低;在快速光响应曲线中,当光照强度高于104 μmol/(m2·s)后,随光照强度的增加,附着水网藻的苦草叶片的rETR显著低于对照,光合作用能力下降;且其饱和光强为248μmol/(m2·s),显著低于对照叶片的685 μmol/(m2·s),表明水网藻附着苦草叶片后导致叶片光响应能力显著下降.     

太湖短吻银鱼的胚胎发育

椭圆卵囊藻培养轮虫时,轮虫平均产卵量均低于４．０个,随着食物浓度增加,后代混交雌体百分率降至１２．５０％．投喂蛋白核小球藻,轮虫的平均产卵量从６．００增加到１１．４０个,后代混交雌体百分率降至４．５８％．小球藻和卵囊藻混合培养轮虫,轮虫平均产卵量少于５．００个,后代个体混交百分率下降．当小球藻浓度增加到８．０＊１０（６）ｃｅｌｌｓ／ｍｌ时,平均产卵量下降至８．３３个,混交百分率也明显升高。     

太湖鲚鱼和鲢、鳙鱼的食物组成及相互影响分析

通过分析和比较太湖鲚鱼、鲢、鳙鱼的食物组成,研究了它们间的食物关系;并结合太湖历年鱼类渔获量资料,对渔获量变化间可能存在的相互影响进行了相关分析.结果表明:在9-12月份期间,鲢鱼、鳙鱼食物中微囊藻约占体积比的90%以上、浮游动物不足10%;用Pianka提出的公式计算鲢鱼和鳙鱼间的食物重叠系数为＞0.99,相同食物组成比例为95.40%.而同期调查0 鲚鱼食物中微囊藻约占体积的15%-35%;如不计算微囊藻仅计算浮游动物的个数组成比则为:枝角类占89.77%±13 69%,桡足类占7.84%±11.53%,轮虫占2.39%±4.95%.而简单相关、偏相关分析表明:鲚鱼和鲢、鳙鱼数量变化间的负相关关系均达到显著水平.分析认为:在富营养化条件下,太湖的鲢鱼和鳙鱼以微囊藻为主要食物;而鲚鱼和鲢、鳙鱼食物中浮游动物的种类基本相同,可能存在对浮游动物资源的激烈竞争;它们种群数量变化间的相互影响是负向的,可能存在互相抑制.     

震级、震源距对地震信号H/V影响分析

本文采用喜马拉雅科学探测台阵四川省境内10个台站,计算了所有地震信号0.5—15 Hz的H/V谱比,分析了震级、震源距对H/V谱比的影响,并与各台站场地地脉动H/V谱比进行了比较. 结果表明: 震级对各台站地震信号的H/V谱比振幅值以及峰值频率几乎没有影响;震源距对谱峰明显的H/V谱比曲线的谱形和峰值频率影响不大,但对谱峰不明显的H/V谱比曲线的峰值频率有一定影响;H/V谱峰明显的台站,地震信号与地脉动信号的H/V谱比曲线的峰值频率具有很高的一致性,H/V谱峰不明显的台站,二者的峰值频率有一定差异.      

The Effects of Mercury Exposure on the Surface Morphology of Gill Filaments in Perna perna (Mollusca: Bivalvia)

This study investigated the possibility that changes in the surface morphology of mussel (Perna perna) gill filaments may be used to indicate the relative toxicity of pollutants in the marine environment. Healthy, adult P. perna were collected and immersed in 2 free-flow tanks. Mercury was added to seawater as it entered tank 1 to achieve a constant level of 50 μg/l⁻¹ over 24 days. Uncontaminated seawater was circulated over the mussels in tank 2 (control) for the same period. A 25 mm² area of gill filament was removed from each of the 5 specimens before and after 24 days immersion in tank 2, and after immersion for 1, 2, 4, 8, 16 and 24 days in tank 1. These were examined using a scanning electron microscope. The remaining soft tissues from each animal were analysed for mercury using an atomic absorption spectrometer. Mercury concentration increased from 0.13 (pre-immersion) to 87 μg/g after day 24. Surface morphology remained normal for all animals in tank 2 and for those exposed to Hg for up to 8 days. However, from 16 to 24 days exposure there was a gradual increase in the diameters of microvilli, a depletion of abfrontal cilia, an increase in abnormal, perhaps necrotic cells and an unusual increase in the number of cilia on the lateral surfaces. These results confirm that P. perna is an efficient bio-accumulator and suggest that their gill pathomorphology may be a useful indicator of toxicity.     

苦草(Vallisneria spiralis)释放的酚酸类物质对铜绿微囊藻(Microcystis aeruginosa)的化感作用

采用广谱性固相萃取小柱富集以10 g( FW)/L的密度培养三天后的苦草(Vallisneria spiralis)种植水,不同溶剂洗脱得到的各组分对铜绿微囊藻(Microcystis aeruginosa)的生长表现出不同程度的抑制,其中甲醇洗脱组分抑藻活性最强.去除该组分中的酚酸后,其抑藻活性下降了22.8％,表明...     

强震前b值与C(n)值变化的对比研究

对比研究了中国西部地区发生的６次中强地震前ｂ值与Ｃ（ｎ）值异常变化特征．６次地震前,ｂ值和Ｃ（ｎ）值都有异常显示．ｂ值异常一般出现较早,最早出现在震前１年左右;Ｃ（ｎ）值异常大多在震前半年至２～３个月出现．ｂ值曲线异常形态一般不太典型,而Ｃ（ｎ）值异常形态较典型,表现为峰值异常．地震前２种异常同时出现．还对计算上述参数的空间范围的选取及窗长和步长的选取进行了讨论．