2017年春季季风间期南海中北部浮游植物生长与微型浮游动物摄食

Phytoplankton growth rates and mortality rates were experimentally examined at 21 stations during the 2017 spring intermonsoon(April to early May) in the northern and central South China Sea(SCS) using the dilution technique, with emphasis on a comparison between the northern and central SCS areas which had different environmental factors. There had been higher temperature but lower nutrients and chlorophyll a concentrations in the central SCS than those in the northern SCS. The mean rates of phytoplankton growth(μ_0) and microzooplankton grazing(m) were(0.88±0.33) d~(–1) and(0.55±0.22) d~(–1) in the central SCS, and both higher than those in the northern SCS with the values of μ_0((0.81±0.16) d~(–1)) and m((0.30±0.09) d~(–1)), respectively.Phytoplankton growth and microzooplankton grazing rates were significantly coupled in both areas. The microzooplankton grazing impact(m/μ_0) on phytoplankton was also higher in the central SCS(0.63±0.12) than that in the northern SCS(0.37±0.06). The microzooplankton abundance was significantly correlated with temperature in the surface. Temperature might more effectively promote the microzooplankton grazing rate than phytoplankton growth rate, which might contribute to higher m and m/μ_0 in the central SCS. Compared with temperature, nutrients mainly affected the growth rate of phytoplankton. In the nutrient enrichment treatment,the phytoplankton growth rate(μn) was higher than μ_0 in the central SCS, suggesting phytoplankton growth in the central SCS was nutrient limited. The ratio of μ_0/μn was significantly correlated with nutrients concentrations in the both areas, indicating the limitation of nutrients was related to the concentrations of background nutrients in the study stations.     

厦门港表层水体磷周转的生物学过程研究Ⅰ. 微型浮游动物对浮游植物的摄食

海水中的磷以三种形式存在:颗粒磷(POP)(生命有机体内的磷和有机碎屑的磷)、溶解有机磷(DOP)和溶解无机磷(DIP)。浮游植物不仅可以吸收溶解无机磷,一些种类还可以吸收溶解有机磷[1];不同粒径的浮游植物对磷酸盐的吸收能力也有所不同,单位叶绿素a的浮游植物对磷酸盐的吸收速率:0.2~3μm浮游植物的吸收速率最大,3~20μm浮游植物的居中,20~200μm浮游植物的最小[2]。磷进入浮游植物以后,又因浮游动物的摄食沿着两条不同的途径向更高的营养级传递。     

春季黄海微型浮游动物对不同粒径浮游植物的摄食速率研究

于2005年3月对黄海海域的7个站位应用稀释法研究了浮游植物的生长率和微型浮游动物对浮游植物的摄食压力。结果表明：实验期间,微型浮游动物生长速率范围在0．34～0．95d-1,浮游植物摄食速率范围在0．44～0．94d-1。微型浮游动物对浮游植物的现存量和初级生产力的摄食压力分别为47．76％～63．80％和61．50...     

三亚湾夏秋两季微型浮游动物摄食研究

根据2005年8月和11月的调查资料,利用现场稀释法,以叶绿素a为检测对象,分别对三亚湾海区夏秋两季微型浮游动物的摄食情况进行研究。结果表明,夏秋两季浮游植物瞬时生长率(k)分别为0.9~1.32/d和1.81~3.30/d,而微型浮游动物的摄食率(g)则为0.85~1.79/d和1.29~2.57/d。对浮游植物现存量和初级生产力的摄食压力分别是,夏季为57.26%~83.30%和78.13%~140.38%,秋季为72.47%~92.35%和86.65%~97.90%。秋季微型浮游动物以微型浮游植物为其主要食物来源,其摄食率和对初级生产力的摄食压力最高,平均分别为1.09/d和107.98%;微型浮游植物的瞬时生长率也是最高的,平均为0.94/d,为浮游植物群体的主要组成部分。在三亚湾夏秋两季可把微型浮游动物作为控制浮游植物生长的重要影响因子之一,同时与秋季相比,夏季微型浮游动物在物质循环和能量流动方面起到更显著的作用。     

渤海微型浮游动物及其对浮游植物的摄食压力

１９９７年６月在渤海的５个站位采样分析了微型浮游动物的空间分布,用稀释法研究了浮游植物的生长率和微型浮游动物对浮游植物的摄食压力。结果表明,所研究的微型浮游动物主要是砂壳纤毛虫和桡足类幼虫。Ｃｏｄｏｎｅｌｌｏｐｓｉｓ ｓｐｐ．是砂壳纤毛虫的绝对优势种,只在１、３、５号站有分布。表层分布为：１号站９８１ｉｎｄ／Ｌ、５号站２００ｉｎｄ／Ｌ、３号站３０ｉｎｄ／Ｌ;垂直分布为上层多、下层少。桡足类幼虫密度     

厦门杏林虾池夏冬季微型浮游动物对浮游植物的摄食压力

20 0 0年 8月和 2 0 0 1年 2月 ,在杏林虾池用稀释法研究了微型浮游动物对浮游植物的摄食压力 .结果表明 :砂壳纤毛虫、甲壳类无节幼体是微型浮游动物的优势种 ;浮游植物生长率夏季为 0 .40～ 1 .0 1 /d、冬季为 0 .1 8～ 0 .96/d ;浮游动物的摄食率夏季为 0 .578～ 1 .3 2 4/d、冬季为 0 .2 0 4～ 0 .2 55/d ;日摄食率 (以C计 )夏季为 1 9.1 7～89.51mg/(m3·d)、冬季为 3 .3 2～ 7.2 3mg/(m3·d) ,各占浮游植物现存量的 43 .90 %～ 73 .40 %、1 8.43 %～ 2 2 .51 %;对初级生产力的摄食压力夏季为 1 1 5.2 3 %～1 93 .52 %、冬季为 3 7.47%～ 1 1 1 .3 1 %.     

南海北部秋季微型浮游动物摄食和种类组成的初步研究

2004年9月到10月间在南海北部海区对微型浮游动物的种类组成进行了调查,同期运用现场稀释法,以叶绿素a为监测对象,估计了该海区内微型浮游动物摄食率和摄食压力的水平。结果表明,南海北部海区纤毛虫群体中以多膜纲寡毛目为主,有16种,其中寡毛亚目纤毛虫4种,砂壳亚目纤毛虫11种。各站纤毛虫丰度比较低,在9~102ind/m3之间。浮游植物瞬时增长率(k)在0.03/d~2.13/d之间;微型浮游动物的摄食率(g)在0.01/d~1.06/d之间。微型浮游动物对浮游植物现存量的摄食压力(Pi)在0.089%~65.23%之间,对初级生产力的摄食压力(Pp)在33.63%~86.04%之间。微型浮游动物的摄食水平主要受其丰度的影响,同时微型浮游动物对浮游植物现存量和初级生产力的摄食压力显示,在南海北部海区微型浮游动物在初级生产力传递方面具有重要的科学意义和研究价值。     

台湾海峡南部夏季微型浮游动物对浮游植物的摄食压力及其生产力

2004年7~8月在台湾海峡南部的5个站位,用稀释法研究了浮游植物的生长率,微型浮游动物对浮游植物的摄食率及其生产力.微型浮游动物主要为无壳纤毛虫,尤其是急游虫类和侠盗虫类.浮游植物的生长率为0.52~0.72/d,浮游动物的摄食率为0.45~1.33/d,相当于每天摄食浮游植物现存量的36%~74%和初级生产力的88%~141%.微型浮游动物的次级生产力(MP02)为初级生产力的28.5%~58.4%.表明微型浮游动物在台湾海峡夏季海洋生态系统的能量流动中发挥着重要的作用.     

三亚湾珊瑚礁海区微型浮游动物种群组成和摄食研究

采用现场稀释法,以叶绿素 a 为检测对象,利用 2006 年 4 月的调查资料对三亚湾珊瑚礁海区微型浮游动物的种群组成和摄食情况进行了研究.结果表明,区内微型浮游动物的组成以纤毛虫为主.微型浮游动物以 Tintinnopsis compressa, Tintinnopsis cylindrical, Tintinnidium semicilidium 三种纤毛虫为优势种,其中 Tintinnopsis 属纤毛虫是优势种群,有 38 种,占总纤毛虫种数的37.3 %.微型浮游动物的摄食率 ( g ) 在 1.28 ～ 2.37 d-1之间,平均值为 0.945 d-1;浮游植物瞬时增长率 ( k ) 为 0.26 ～ 1.44 d-1,平均值为 1.963 d-1;微型浮游动物对浮游植物现存量和初级生产力的摄食压力分别在 72.20 % ～90.65 % 和 113.31 % ～ 315.34 %,平均值分别为 84.56 % 和 177.05 %.三亚湾珊瑚礁海区内,微型浮游动物的摄食水平主要取决于水体中微型浮游动物和叶绿素a之间的相对数量关系.在控制浮游植物生长和转移浮游植物生产力方面微型浮游动物起着相当重要的作用,可以在一定程度上降低潜在的富营养化威胁.     

海蜇养殖港塭微型浮游动物的摄食和生产力研究

通过对山东省靖海湾海蜇养殖港塭定期采样,采用稀释法研究该海蜇养殖港塭水体中浮游植物的生长率、微型浮游动物对浮游植物的摄食率、摄食压力以及微型浮游动物的生产力。研究结果表明,海蜇养殖港塭微型浮游动物组成比较简单,海蜇养殖期间微型浮游动物丰度低于海蜇捕捞结束期。其中,海蜇养殖期间微型浮游动物的优势种为根状拟铃虫(Tintinnopsis radix),为600~2 600 ind/L,而海蜇捕捞结束后优势种为根状拟铃虫、诺氏麻铃虫(Leprotintinnus nordquisti)和运动类铃虫(Codonellopsis mobilis),丰度分别为3 000~6 000、1 500~3 0001、500~3 000 ind/L。研究期间,该港塭浮游植物生长率为0.05~1.03 d-1。微型浮游动物的摄食率为0.24~2.37 d-1,对浮游植物现存量的摄食压力为21.10%~90.61%,对潜在初级生产力的摄食压力为77.08%~583.68%,而微型浮游动物的次级生产力占初级生产力的22.92%~76.92%。本研究表明微型浮游动物在海蜇养殖港塭生态系统物质和能量流动中起着重要作用。     

Grazing impact of microzooplankton on phytoplankton in the Xiamen Bay using pigment-specific dilution technique

Phytoplankton group-specific growth and microzooplankton grazing were determined seasonally using the dilution technique with high-performance liquid chromatography （HPLC） in the Xiamen Bay, a subtropical bay in southeast China, between May 2003 and February 2004. The results showed that growth rates of phytoplankton ranged from 0.71 to 2.2 d^-1 with the highest value occurred in the inner bay in May. Mierozooplankton grazing rates ranged from 0.5 to 3.1 d^-1 with the highest value occurred in the inner bay in August. Microzooplankton grazing impact ranged from 39% to 95% on total phytoplankton Chl a biomass, and 65% to 181% on primary production. The growth and grazing rates of each phytoplankton group varied, the highest growth rate （up to 3.3 d^-1 ） was recorded for diatoms in August, while the maximum grazing rate （ up to 2.1 d ^-1 ） was recorded for chlorophytes in February in the inner bay. Among main phytoplankton groups, grazing pressure of microzooplankton ranged from 10% to 83% on Chl a biomass, and from 14% to 151% on primary production. The highest grazing pressure on biomass was observed for cryptophytes （83%） in August, while the maximum grazing pressure on primary production was observed for eyanobacteria （up to 151% ） in December in the inner bay. Net growth rates of larger phytoplanktons （diatoms and dinoflagellates） were higher than those of smaller groups （ prasinophytes, chlorophytes and cyanobacteria）. Relative preference index showed that microzooplankton grazed preferentially on prasinophytes and avoided to harvest diatoms in cold seasons （December and February）.     

Preliminary study on different nutrient pools supplies for the phytoplankton growth in the Jiaozhou Bay in China in the fall of 2004

The source and significance of two nutrients, nitrogen and phosphorous, were investigated by a modified dilution method performed on seawater samples from the Jiaozhou Bay, in autumn 2004. This modified dilution method accounted for the phytoplankton growth rate, microzooplankton grazing mortality rate, the internal and external nutrient pools, as well as nutrient supplied through remineralization by microzooplankton. The results indicated that the phytoplankton net growth rate increased in turn from inside the bay, to outside the bay, to in the Xiaogang Harbor. The phytoplankton maximum growth rates and microzooplankton grazing mortality rates were 1.14 and 0.92 d-1 outside the bay, 0.42 and 0.32 d-1 inside the bay and 0.98 and 0.62 d-1 in the harbor respectively. Outside the bay, the remineralized nitrogen (Kr=24.49) had heavy influence on the growth of the phytoplankton. Inside the bay, the remineralized phosphorus(Kr=3.49) strongly affected the phytoplankton growth. In the harbor, the remineralized phosphorus (Kr=3.73) was in larger demand by phytoplankton growth. The results demonstrated that the different nutrients pools supplied for phytoplankton growth were greatly in accordance with the phytoplankton community structure, microzooplankton grazing mortality rates and environmental conditions. It is revealed that nutrient remineralization is much more important for the phytoplankton growth in the Jiaozhou Bay than previously believed.     

Growth and grazing rate dynamics of major phytoplankton groups in an oligotrophic coastal site

There has been more attention to phytoplankton dynamics in nutrient-rich waters than in oligotrophic ones thus requiring the need to study the dynamics and responses in oligotrophic waters. Accordingly, phytoplankton community in Blanes Bay was overall dominated by Prymnesiophyceae, remarkably constant throughout the year (31 ± 13% Total chlorophyll a, Tchl a) and Bacillariophyta with a more episodic appearance (20 ± 23% Tchl a). Prasinophyceae and Synechococcus contribution became substantial in winter (Prasinophyceae = 30% Tchl a) and summer (Synechococcus = 35% Tchl a). Phytoplankton growth and grazing mortality rates for major groups were estimated by dilution experiments in combination with high pressure liquid chromatography and flow cytometry carried out monthly over two years. Growth rates of total phytoplankton (range = 0.30–1.91 d⁻¹) were significantly higher in spring and summer (μ > 1.3 d⁻¹) than in autumn and winter (μ ∼ 0.65 d⁻¹) and showed a weak dependence on temperature but a significant positive correlation with day length. Microzooplankton grazing (range = 0.03–1.4 d⁻¹) was closely coupled to phytoplankton growth. Grazing represented the main process for loss of phytoplankton, removing 60 ± 34% (±SD) of daily primary production and 70 ± 48% of Tchl a stock. Chla synthesis was highest during the Bacillarophyceae-dominated spring bloom (Chl a_synt = 2.3 ± 1.6 μg Chl a L⁻¹ d⁻¹) and lowest during the following post-bloom conditions dominated by Prymnesiophyceae (Chl a_synt = 0.23 ± 0.08 μg Chl a L⁻¹ d⁻¹). This variability was smoothed when expressed in carbon equivalents mainly due to the opposite dynamics of C:chl a (range = 11–135) and chl a concentration (range = 0.07–2.0 μg chl a L⁻¹). Bacillariophyta and Synechococcus contribution to C fluxes was higher than to biomass because of their fast-growth rate. The opposite was true for Prymnesiophyceae.     

Studies on growth rate and grazing mortality rate by microzooplankton of size-fractionated phytoplankton in spring and summer in the Jiaozhou Bay, China

1 Introduction Phytoplankton has been considered as a dom inantprim ary producer in m arine ecosystem s, starting them arine food chain (N ing and V aulot.,2003;Sun etal.,2001; Zhu et al., 2000; N ing and V aulot, 1992). A l-though potentialfates ofphytoplankton include advec-tion,verticalm ixing,sinking and m ortality due to virallysis and grazing (B anse,1994),m ortality due to graz-ing,especially by m icrozooplankton,is generally con- μm m esh to 25-L carboys, then transpo…     

海洋浮游细菌生长率和被摄食的研究综述

海洋浮游细菌利用海水中的溶解有机碳合成自身物质,是海洋浮游生态系统的二次生产者。微型浮游动物是细菌的主要摄食者,也是细菌生产向较高营养级传递的中介。研究海洋浮游细菌的生长率和被(微型浮游动物的)摄食率对理解海洋浮游生态系统的功能具有重要作用。本文综述了利用改变海水中生物类群组成(或功能)的培养方法研究海洋浮游细菌生长率和被摄食率的历程和现状,为我国的同类研究提供借鉴。改变海水中生物类群组成(或功能)进行培养的方法有海水分粒级培养、海水稀释培养和添加选择性抑制剂培养。这些方法各有其局限性,应用并不广泛。细菌及其主要摄食者异养鞭毛虫群落在自然海区和实验室内都有生长周期,鞭毛虫的生长周期落后于细菌,因此细菌的生长率有时会小于被摄食率,有时会大于被摄食率。我国这方面的研究相对落后,应值得引起重视,建议从海水稀释培养法入手开展相关研究。     

台湾海峡南部夏季微型浮游动物对不同色素类群浮游植物的摄食压力

2005年7月在台湾海峡南部4个站位应用“稀释法”结合高效液相色谱(HPLC)色素分析技术研究了不同色素类群浮游植物的生长率及微型浮游动物对其的摄食死亡率.结果表明,不同色素类群浮游植物的生长率(k)和摄食死亡率(g)分别为0.52～ 1.34 d-1和0.25 ～ 1.10 d-1,微型浮游动物对不同色素类群浮游植物的现存量和初级生产力的摄食压力分别为22％～ 66％和40％～ 151％.通过比较不同类群浮游植物的g/k值,发现颗粒较大的浮游植物(硅藻和甲藻)的净生长率要大于那些微型藻类(蓝细菌、隐藻和定鞭金藻等)的净生长率,说明本次研究中微型藻类更易受到微型浮游动物的摄食控制.     

冬季南海北部海域微型浮游动物及其对浮游植物摄食压力研究

2009年1月在南海北部海域的5个站位,采用稀释法和显微分析技术研究了浮游植物生长率及微型浮游动物对浮游植物的摄食压力,同时测定了微型浮游动物的丰度及类群组成.结果表明:南海北部微型浮游动物类群主要以无壳纤毛虫为主,南海北部微型浮游动物类群细胞丰度为33～529个/dm3.南海北部浮游植物生长率为0.45～1.83 d-1,微型浮游动物摄食率为0.44～1.76 d-1,摄食压力占浮游植物现存量的42.6%～82.8%,占初级生产力的97.3%～225.1%.近岸区摄食压力比陆架区高,表明冬季南海近岸区微型浮游动物摄食能够有效的控制浮游植物的生长,而陆架区浮游植物生长率大于摄食率,浮游植物存在着现存量的积累,微型浮游动物并不能完全控制浮游植物的生长.