~(14)C真空玻璃系统的技术改造

本文提出了~(14)C真空玻璃系统的一些技术关键。并详细论述了应用这些技术关键对本实验室原有系统的改进方法。经实验证明改进后的系统性能良好,结构简单,操作使用方便,是一种实用性很强的真空玻璃系统。本改进方法对于其他炎似真空玻璃系统也具有一定的参考价值。     

基于感应电流测量的海峡水通量海底缆线观测系统设计

首先回顾了基于海底缆线的海峡水通量观测理论方法的发展历程,对基于电压测量的水通量反演方法进行了梳理,重点阐述了其中的关键观测要素和主要误差来源。为了提升系统可靠性、摒弃外界因素干扰,提出了一种基于感应电流测量的观测方法。对比传统方法,分析了本方法的优缺点,评估了基于本方法的海洋观测系统建设需求和可能性。最后应用本系统建立的一个理想海域——青岛胶州湾湾口各类海洋要素的典型取值范围,通过合理的特征尺度估算和量纲分析,估计了基于电流测量的观测系统中的各类设备的参数要求,其中关键测量器材——电流表至少应满足观测量程覆盖10~(–1)~10~1 m A,观测精度大于1μA,取样频率大于1/60 Hz。在电学测量仪器中,适应本系统要求的器材较普遍,可选择的测量仪器种类较丰富。     

500m深度拖曳系统的设计与试验

针对500 m波浪式拖曳系统的技术要求,介绍了拖体、流线型拖缆、电控绞车和总控程序4个关键部分的设计;根据海上的试验情况,分析了拖体的缆深比和定深拖曳等数据,表明本系统达到预期要求,可以为上层海洋学的调查与观测提供良好的平台。     

侏儒蛤TRAF基因家族的研究

双壳软体贝类缺乏获得性免疫,因此先天免疫防御系统在其应对外界病原刺激时发挥着至关重要的作用.其中,肿瘤坏死因子受体相关因子(TRAF)作为保守的参与先天免疫反应的关键蛋白,可通过与TLR、TNFR等不同关键跨膜受体相结合,特异性识别不同病原并启动相应的免疫应答.本研究以侏儒蛤(Mulinia lateralis)为研究...     

MG－1型海缆埋设系统总体设计

ＭＧ－１型海缆埋设系统是专为埋设中日海底光缆研制的。本系统装备于“邮电１号”敷缆船上，是一个经济、高效、具有现代化测控系统的犁埋式作业系统。１９９３年仅用１３个小时就顺利地完成了中日海底光缆中国侧９０ｋｍ的埋设工作。本系统于１９９４年６月通过技术鉴定。１９９５年获得邮电部三等奖。ＭＧ－１系统除可用于埋设海底通信电缆外，也可用于埋设其它海底电缆。本文对系统总体设计中考虑的问题、系统的设计结果以及试验和施工概况作了介绍     

多波束声纳计量测试系统设计

为满足多波束声纳量值溯源与传递需求,设计多波束声纳关键参数的计量测试系统。基于大比尺原型深水港池和多维运行控制机构,通过水下横向测距代替垂向测深的方式对多波束声纳进行了测深准确度与有效条带宽度的计量测试。阐述了测试系统的主要组成和测试方法,给出了本测试系统测深结果的扩展不确定度。参考标准值与示值(或标称值)比对结果表明,被检多波束声纳测深示值误差小于0.2%,条带宽度误差小于7%。为规范水下声纳设备技术指标校准,指导多波束声纳计量标准建设提供参考。     

深海照相系统

从美国本萨斯公司进口的377型深海照相系统,于85年3月底在天津海洋技术研究所验收完毕。该型深海照相系统,在国际上有一定声誉,被很多国家采用,并在锰结核的调查中发挥了一定作用。本系统由下述部分组成:377型调查照相机二台,383型高强度闪光灯二只,391主电源一台,392辅助电源一台,     

海洋无脊椎动物酚氧化酶的研究进展

海洋无脊椎动物缺乏真正意义上的抗体,没有免疫记忆,只能靠非特异性免疫系统防御和抵抗病原体的感染.酚氧化酶原激活系统是海洋无脊椎动物非特异性免疫系统中至关重要的一员,而酚氧化酶作为该系统末端的一种含铜金属酶,则通过催化黑化反应在海洋无脊椎动物非特异性免疫防御中发挥了关键的作用.本文结合作者已有的研究成果并综合本领域的大量参考文献对酚氧化酶原激活系统和酚氧化酶的免疫学功能、激活机制、生化性质与酶性质以及基因与分子结构等方面的研究进展进行综述.     

大西洋鲑在封闭循环水养殖模式下的生长和性腺发育研究

本研究探讨了在封闭循环水养殖模式下大西洋鲑(Salmosalar)的生长发育模式,对封闭循环水系统养殖的大西洋鲑重要生长数量性状进行生物学测量,并跟踪性腺发育状况。本研究自2012年1月通过封闭循环水系统进行大西洋鲑养殖,经过周年养殖,体质量平均达到1194.42g,体长平均达到417.00 mm,体高平均达到100.58 mm,体宽平均达到53.52 mm,少数个体在养殖10个月后性腺发育较快,性腺质量达到9.76 g,性腺指数达到1.31%。本研究的研究结果为建立大西洋鲑标准化循环水养殖系统积累资料。     

利用面向对象的技术开发海洋环境预报发布系统

本文对面向对象的技术作了简单介绍,详细地叙述了本台海洋环境预报发布系统的性能结构和实现方法,介绍面向对象的技术在本系统中的应用。     

Daily variability of dissolved inorganic radiocarbon in Sargasso Sea surface water

Surface water samples were collected daily in June 2000 at a site in the Sargasso Sea to observe variability of Δ¹⁴C values in dissolved inorganic carbon (DIC). Temperature, salinity, DIC concentration, alkalinity, and δ¹³C and Δ¹⁴C values of DIC were measured in the samples. Ten Δ¹⁴C measurements averaged 81 ± 8‰ and had a range of 24‰ over the sixteen-day cruise. Δ¹⁴C values were more variable during the latter half of the cruise. Salinity and temperature measurements in the mixed layer throughout the cruise indicate that there were changes in water mass that occurred at our site. We conclude that the daily range of DIC Δ¹⁴C values in the surface ocean at our site is several times greater than the annual change in surface waters in the Sargasso Sea during the last two decades of the 20th century. This points to the importance of obtaining multiple measurements of the surface ocean to adequately define the true variability of DIC Δ¹⁴C measurements.     

In vitro O2 fluxes compared with 14C production and other rate terms during the JGOFS Equatorial Pacific experiment

We report rates of gross and net O₂ production measured in vitro during JGOFS cruises in the equatorial Pacific in spring and fall, 1992. We scale O₂ productivities to net and gross C production. We then compare the calculated rates with ¹⁴C production and with new/export production measured by various techniques. ¹⁴C productivities in samples incubated for 24 h are about 45% of gross carbon production rates calculated from gross O₂ production. The difference is compatible with expected rates of the Mehler reaction, photorespiration, excretion, and community mitochondrial respiration. ¹⁴C production rates are similar to net carbon production rates in the upper half of the euphotic zone. At lower irradiances, where net C production can be zero or less, ¹⁴C productivities lie between net community production and gross primary production. Net carbon production rates in vitro are a factor of =4–20 times greater than estimates from drifting sediment trap and tracer transport studies. This difference probably reflects anomalous accumulation of POC in bottles because of the exclusion of grazers.     

Constraining the propagation of bomb-radiocarbon through the dissolved organic carbon (DOC) pool in the northeast Pacific Ocean

This study extends the 1991-1995 records of marine dissolved organic carbon (DOC) concentrations and Δ¹⁴C values at hydrographic Station M (34°50′N, 123°00′W) with new measurements from a frozen (-20 °C) archive of samples collected between April 1998 and October 2004. The magnitudes and synchronicity of major Δ¹⁴C anomalies throughout the time-series imply transport of DOC from the surface ocean to depths of at least 450 m on the timescale of months. Keeling plots of all measurements at Station M predict a continuum of possible background DOC compositions containing at least 21 μM of -1000‰ (i.e., ≥57,000 ¹⁴C years) DOC, but are more consistent with mean deep DOC (38 μM, -549‰; i.e., 6,400 ¹⁴C years). These results and coral records of surface dissolved inorganic carbon (DIC) Δ¹⁴C were used to estimate pre-bomb DOC Δ¹⁴C depth profiles. The combined results indicate that bomb-¹⁴C has penetrated the DOC pool to depths of ≥450 m, though the signal at that depth is obscured by short-term variability.     

MEASUREMENT OF PRIMARY PRODUCTION IN AN ENCLOSED WATER COLUMN

A CEE (2.5 m by 16 m) has been used to study the budget of organic production by measuring the changes in POC/N, oxygen and 14CO, uptake through three-week period. 14C primary production measurements were conducted with 4-hours and 24-hours incubation periods, and with size fractionation. Different types and sizes of bottle effects were examined. Results showed that the oxygen method production was highest, followed by the 14CO2 uptake method, and POC gain showed the lowest. The value of PQ should be more than 1.7. The ratio of 4-hour incubation to 24-hour incubation was 2.42±0.22, indicating that net daily production is equal to 9.7±0.9 h of illuminated growth. Different types and sizes of bottles had little effect on primary production.     

Photochemical changes in chemical markers of sedimentary organic matter source and age

Several chemical markers of organic matter source and age are shown to be susceptible to light-induced alteration. To test for the photochemical lability of markers previously employed for sediments from the Louisiana coastal zone, we subjected sediments under resuspension conditions to simulated sunlight, and monitored changes in C:N and Br:OC ratios, δ¹³C, Δ¹⁴C, and lignin composition. Markers of terrigenous origin (high C:N, lignin) decreased and δ¹³C became enriched in sediments containing primarily terrigenous organic matter, while a marker of marine organic matter (Br:OC) decreased in samples containing significant contributions from this source. Preferential loss of ¹⁴C from all sediments indicated enhanced photochemical lability of organic matter of relatively recent origin, consistent with the changes in chemical markers. Most, but not all, experimental alterations are consistent with field distributions of these markers. Relatively small experimental changes in the markers in combination with confounding processes in the environment, however, prevent these parallel trends from providing any more than a consistency test for the importance of photochemical reactions in this region.     

Molecular and isotopic constraints on the sources of suspended particulate organic carbon on the northwestern Atlantic margin

The abundance, carbon isotopic composition (Δ¹⁴C and δ¹³C), and lipid biomarker (alkenones and saturated fatty acids) distributions of suspended particulate organic matter were investigated at three stations centered on the 2000, 3000, and 3500 m isobaths over the New England slope in order to assess particulate carbon sources and dynamics in this highly productive and energetic region. Transmissometry profiles reveal that particle abundances exhibit considerable fine structure, with several distinct layers of elevated suspended particulate matter concentration at intermediate water depths in addition to the presence of a thick bottom nepheloid layer at each station. Excluding surface water samples, the Δ¹⁴C values of particulate organic carbon (POC) indicated the presence of a pre-aged component in the suspended POC pool (Δ¹⁴C<+38‰). The Δ¹⁴C values at the 3000 m station exhibited greater variability and generally were lower than those at the other two stations where the values decreased in a more systematic matter with increasing sampling depth. These lower Δ¹⁴C values were consistent with higher relative abundances of terrigenous long-chain fatty acids at this station than at the other two stations. Two scenarios were considered regarding the potential provenances of laterally transported POC: cross-shelf transport of shelf sediment (Δ¹⁴C=?140‰) and along-slope transport of the slope sediment proximal to the sampling locations (Δ¹⁴C=?260‰). Depending on the scenario, isotopic mass balance calculations indicate allochthonous POC contributions ranging between 15% and 54% in the meso- and bathy-pelagic zone, with the highest proportions at the 3000 m station. Alkenone-derived temperatures recorded on suspended particles from surface waters closely matched in-situ temperatures at each station. However, alkenone-derived temperatures recorded on particles from the subsurface layer down to 250 m were lower than those of overlying surface waters, especially at the 3000 m station, implying supply of phytoplankton organic matter originally produced in cooler surface waters. AVHRR images and temperature profiles indicate that the stations were under the influence of a warm-core ring during the sampling period. The low alkenone-derived temperatures in the subsurface layer coupled with the lower Δ¹⁴C values for the corresponding POC suggests supply of OC on resuspended sediments underlying cooler surface waters distal to the study area, possibly further north or west. Taken together, variations in Δ¹⁴C values, terrigenous fatty acid abundances, and alkenone-derived temperatures among the stations suggest that input of laterally advected OC is a prominent feature of POC dynamics on the NW Atlantic margin, and is spatially heterogeneous on a scale smaller than the distance between the stations (<150 km).     

Daily variability of dissolved inorganic radiocarbon at three sites in the surface ocean

We report radiocarbon measurements of dissolved inorganic carbon (DIC) in surface water samples collected daily during cruises to the central North Pacific, the Sargasso Sea and the Southern Ocean. The ranges of Δ¹⁴C measurements for each cruise (11–30‰) were larger than the total uncertainty (7.8‰, 2-sigma) of the measurements. The variability is attributed to changes in the upper water mass that took place at each site over a two to four week period. These results indicate that variability of surface Δ¹⁴C values is larger than the analytical precision, because of patchiness that exists in the DIC Δ¹⁴C signature of the surface ocean. This additional variability can affect estimates of geochemical parameters such as the air–sea CO₂ exchange rate using radiocarbon.     

Unreliable 14C dating of long-stored deep-sea sediments due to bacterial activity

Unreliability of radiocarbon ages obtained on the organic-carbon fraction of marine sediments stored wet and over a long period is explained as a consequence of bacterial up-take of recent CO₂. Deep-freezing, drying or immediate processing of samples intended for ¹⁴C age determination is recommended.     

Determination of volatile fatty acid turnover rates in organic-rich marine sediments

Volatile fatty acid (VFA) apparent turnover rates in organic-rich marine sediments were determined by measuring whole sediment VFA concentration and the corresponding first-order reaction rate constants. In order to measure VFA concentrations, bulk wet sediment samples were basified, freeze-dried, extracted with methanol, derivatized to form methyl esters of the VFAs, and analyzed by packed-column gas chromatography using hexanoic acid as an internal standard. The detection limits for acetate, propionate, iso-butyrate and butyrate were 1.0, 0.4, 0.2 and 0.2 μmol l^?1_s, respectively, for 600 ml samples. Rate constants for acetate and propionate were determined by anaerobically incubating samples at in-situ temperatures with tracer levels of ¹⁴C-labelled VFAs. Metabolized label was recovered as CO₂, CH₄, cellular material, water-soluble material, and VFA (ether-soluble) fractions. Apparent turnover rates measured during summer and winter in anoxic Cape Lookout Bight, North Carolina (U.S.A.) sediments were in the range of 19–330 μmol l^?1_s h^?1 for acetate and 0.7–7.0 μmol l^?1_s h^?1 for propionate.     

Impact of land use on the faecal microbial quality of hill‐country streams

Faecal contamination of rural streams is of increasing concern in New Zealand. This study assessed hill‐country streams in the Whatawhata district that were impacted by pastoral farming, indigenous forest, or Pinus radiata forest; by measuring Escherichia coli bacteria at 14 sampling sites fortnightly for 2 years. E. coli concentrations were highest in streams flowing through grazed pasture. In both years there was a noticeable seasonal pattern in all streams irrespective of land use, with highest bacterial concentrations in summer and autumn and lowest in winter and early spring. There was no obvious correlation between E. coli concentration and rainfall or stream flow. In those streams impacted by a change in land use from pastoral to pines during the study, E. coli concentration fell rapidly and remained at levels lower than those in streams impacted by either indigenous or 7‐year pine forests. As E. coli was detected in all but two samples, the water in these streams is not suitable for human consumption. The pastoral streams consistently failed to meet stock drinking‐water guidelines (median concentration not greater than 100 E. coli 100 ml^–1) and the forest streams failed to do so in summer. Twenty‐eight percent of pastoral samples, 25% of indigenous forest samples, 14% of 7‐year pine forest samples, and 5% in New Pines stream samples (after planting) had E. coli concentrations associated with a high level of risk for contact recreation (>500 E. coli 100 ml^‐1) and the high concentrations usually occurred in summer.