Changes in microbial community composition in the leaf litter of successional communities after volcanic eruptions of Mount Usu,northern Japan

Changes in the fungal and bacterial biomass and community structure in litter after the volcanic eruptions of Mount Usu, northern Japan were investigated using a chronosequence approach, which is widely used for analyzing vegetation succession. The vegetation changed from bare ground(10 years after the eruptions) with little plant cover and poor soil to monotonic grassland dominated by Polygonum sachalinense with undeveloped soil(33 years) and then to deciduous broad-leaved forest dominated by Populus maximowiczii with diverse species composition and well-developed soil(100 years). At three chronosequential sites, we evaluated the compositions of phospholipid fatty acids(PLFAs), carbon(C) and nitrogen(N) contents and the isotope ratios of C(δ13C) and N(δ15N) in the litter of two dominant species, Polygonum sachalinense and Populus maximowiczii. The C/N ratio, δ13C and δ15N in the litter of these two species were higher in the forest than that in the bare ground and grassland. The PLFAs gradually increased from the bare ground to the forest, showing that microbial biomass increased with the development of the soil and/or vegetation. The fungi-to-bacteria ratio of PLFA was constant at 5.3 ± 1.4 in all three sites, suggesting that fungi were predominant. A canonical correspondence analysis suggested that the PLFA composition was related tothe successional ages and the developing soil properties(P 0.05, ANOSIM). The chronosequential analysis effectively detected the successional changes in both microbial and plant communities.     

Characterization of microbial community structure in rhizosphere soils of Cowskin Azalea (Rhododendron aureum Georgi) on northern slope of Changbai Mountains,China

The vegetation and soil are mutual environmental factors, soil characteristics, such as chemical properties and microorganism that affect the vegetation occurrence, development and succession speed. In this study, we evaluated the structure of microbial communities of rhizosphere of Cowskin Azalea(Rhododendron aureum Georgi) populations and compared with non-rhizosphere soils at four sample sites of the Changbai Mountains, China, and analyzed the correlation between chemical properties of soil and microbial communities. The results showed that microbial structure and soil chemical properties are significant superior to non-rhizosphere at all four sample sites. The rhizosphere microorganisms are mainly composed of bacteria, actinomycetes, followed by fungi least. The principal component analysis(PCA) biplot displayed that there are differences between rhizosphere and non-rhizosphere soils for microflora; Through correlation analysis, we found that the bacteria is clearly influenced by p H on the Changbai Mountains, besides p H, other soil features such as NO3–-N. These data highlight that R. aureum as the dominant vegetation living in the alpine tundra is a key factor in the formation of soil microorganism and improving soil fertility, and is of great significance for the maintenance of alpine tundra ecosystem.     

Electrochemical properties of electrodes with different shapes and diffusion kinetic analysis of microbial fuel cells on ocean floor

Microbial fuel cell(MFC) on the ocean floor is a kind of novel energy-harvesting device that can be developed to drive small instruments to work continuously.The shape of electrode has a great effect on the performance of the MFC.In this paper,several shapes of electrode and cell structure were designed,and their performance in MFC were compared in pairs:Mesh(cell-1) vs.flat plate(cell-2),branch(cell-3) vs.cylinder(cell-4),and forest(cell-5) vs.disk(cell-6) FC.Our results showed that the maximum power densities were 16.50,14.20,19.30,15.00,14.64,and 9.95 mWm-2 for cell-1,2,3,4,5 and 6 respectively.And the corre-sponding diffusion-limited currents were 7.16,2.80,18.86,10.50,18.00,and 6.900 mA.The mesh and branch anodes showed higher power densities and much higher diffusion-limited currents than the flat plate and the cylinder anodes respectively due to the low diffusion hindrance with the former anodes.The forest cathode improved by 47% of the power density and by 161% of diffusion-limited current than the disk cathode due to the former’s extended solid/liquid/gas three-phase boundary.These results indicated that the shape of electrode is a major parameter that determining the diffusion-limited current of an MFC,and the differences in the elec-trode shape lead to the differences in cell performance.These results would be useful for MFC structure design in practical applica-tions.     

Comparative study of two carbon fiber cathodes and theoretical analysis in microbial fuel cells on ocean floor

Cathode activity plays an important role in the improvement of the microbial fuel cells on ocean floor(BMFCs). A comparison study between Rayon-based(CF-R) and PAN-based carbon fiber(CF-P) cathodes is conducted in the paper. The two carbon fibers were heat treated to improve cell performance(CF-R-H CF-P-H), and were used to build a new BMFCs structure with a foamy carbon anode. The maximum power density was 112.42 mW m-for CF-R-H, followed by 66.6 mW m-2 for CF-R, 49.7 mW m-2 for CF-P-H and 21.6 mW m-2 for CF-P respectively. The higher specific area and deep groove make CF-R have a better power output than with CF-P. Meanwhile, heat treatment of carbon fiber can improve cell power, nearly two-fold higher than heat treatment of plain fiber. This improvement may be due to the quinones group formation to accelerate the reduction of oxygen and electron transfer on the fiber surface in the three phase boundary after heat treatment. Compared to PAN-based carbon fiber, Rayon-based carbon fiber would be preferentially selected as cathode in novel BMFCs design due to its high surface area, low cost and higher power. The comparison research is significant for cathode material selection and cell design.     

Molecular detection of microbial communities associated with Microcystis vs Synechococcus dominated waters in Tianjin,China

Harmful cyanobacterial blooms cause many ecological disasters worldwide. During the development of cyanobacterial blooms, the diversity and domination of cyanobacterial taxa are of a particular concern. In this study, the microbial community structure within a water system, such as in Yuqiao Reservoir and Haihe River in Tianjin City, China, was compared by using next-generation sequencing. A total of 5 001 operational taxonomic units were obtained and clustered from filtered 16 S rDNA V3–V4 region sequences. The cyanobacterial and microbial structures greatly differed in these two water areas. Microcystis was dominant in Yuqiao, whereas Synechococcus was dominant in Haihe. Proteobacteria species were dominant among all detected samples. The relative abundances of Bacteroidetes and Planctomycetes were higher in Yuqiao Reservoir than in Haihe River, whereas Firmicutes and Verrucomicrobia were relatively abundant in Haihe River. Further analyses indicated that the domination of both cyanobacteria was strongly related to several environmental factors, such as total nitrogen, total phosphorus, and dissolved oxygen, reflecting the role of trophic states in shaping the dominance of cyanobacterial taxa. The present study provided the example for Microcystis and Synechococcus dominance along a cyanobacterial bloom in north China. Applying highthroughput sequencing could offer a wide field of vision in analyzing microbial community structures.     

Characteristics of rock fragments in different forest stony soil and its relationship with macropore characteristics in mountain area,northern China

Rock fragments have major effect on soil macropores and water movement. However, the characteristics of rock fragments and their relationship with macropore characteristics remain elusive in forest stony soils in northern mountainous area of China. The objectives of this study are to (1) use Industrial Computed Tomography (CT) scanning to quantitatively analyze rock fragment characteristics in intact soil columns in different forest lands and (2) identify the relationship between characteristics of rock fragments and that of the macropores. Intact soil columns that were 100 mm in diameter and 300 mm long were randomly taken from six local forest stony soils in Wuzuolou Forest Station in Miyun, Beijing. Industrial CT was used to scan all soil column samples, and then the scanned images were utilized to obtain the three-dimensional (3D) images of rock fragments and macropore structures. Next, the parameters of the rock fragments and macropore structure were measured, including the volume, diameter, surface area, and number of rock fragments, as well as the volume, diameter, surface area, length, angle, tortuosity and number of macropores. The results showed that no significant difference was found in soil rock fragments content in the 10-30 cm layer between mixed forest and pure forest, but in the 0-10 cm soil layer, the rock fragments in mixed forest were significantly less than in pure forest. The number density of macropores has significant negative correlation with the number of rock fragments in the 0-10 cm soil layer, whereas this correlation is not significant in 10-20 cm and 20-30 cm soil layers. The volume density of macropore was not correlated with the volume density of rock fragments, and there is no correlation between the density of macropore surface area and the density of rock fragment surface area. Industrial CT scanning combined with image processing technology can provide a better way to explore 3D distribution of rock fragments in soil. The content of rock fragments in soil is mainly determined by parent rocks. The surface soil (0-10 cm) of forest contains fewer rock fragments and more macropores, which may be caused by bioturbation, root systems, gravitational settling and faunal undermining.     

Morphological characteristics and environmental implications of phytoliths in topsoils from different vegetation zones on northern slope of Changbai Mountains,China

The Changbai Mountains,located in the temperate monsoon climate zone of East Asia,is an ideal loca-tion for the research on timberline response to global changes.In this study,the topsoils were collected from different vertical vegetation zones on the northern slope of the Changbai Mountains,Northeast China in August 2009,and phytoliths in the soil samples were extracted by using wet oxidation method and identified with Motic 2.0 microscope in laboratory.The results show that phytoliths are abundant in the ...     

Selective feeding of bay scallop Argopecten irradians on phytoplankton community revealed by HPLC analysis of phytopigments in Bohai Sea,China

Understanding the feeding selectivity on phytoplankton by shellfish is currently a big challenge.In order to investigate the feeding behavior of bay scallop(Argopecten irradians)on phytoplankton,we compared its compositions of phytopigments in digestive glands with those in the surrounding seawater,and conducted five consecutive investigations between July and November 2016 in a bay scallop culture area along coast of Qinghuangdao City,northwest of the Bohai Sea,China.Phytopigments in four-size fractionated phytoplankton of seawater(micro-(20-200 μm);nano(L)-[10-20 μm];nano(S)-[2.7-10 μm],and pico-[2.7 μm])and digestive glands of A.irradians were examined to investigate the selective feeding of A.irradians.Results show that fucoxanthin and peridinin constituted the major part of taxonomically diagnostic carotenoids(TDCs)in the micro-and nano(L)-phytoplankton in seawater.Compared with total phytoplankton biomass of seawater(TPB,sum of the four sizes),a substantial decrease of fucoxanthin proportion to total DCs in digestive glands was observed while that of peridinin,19'-butanoyloxyfucoxanthin,alloxanthin and 19'-hexanoyloxy-fucoxanthin showed an obvious increase when those pigments were mainly confined to micro-sized phytoplankton(20-200 μm).However,zeaxanthin and prasinoxanthin were mainly confined to nano(s)-and pico-phytoplankton,of which the proportions in digestive glands were usually lower in TPB.The contribution of lutein to total DCs in digestive glands(with an average of 7.23%)increased compared with TPB of seawater(with an average of 0.63%)during all five sampling times.     

Response of fuzzy clustering on different threshold determination algorithms in spectral change vector analysis over Western Himalaya,India

Change detection is a standard tool to extract and analyze the earth's surface features from remotely sensed data. Among the different change detection techniques, change vector analysis(CVA) have an exceptional advantage of discriminating change in terms of change magnitude and vector direction from multispectral bands. The estimation of precise threshold is one of the most crucial task in CVA to separate the change pixels from unchanged pixels because overall assessment of change detection method is highly dependent on selected threshold value. In recent years, integration of fuzzy clustering and remotely sensed data have become appropriate and realistic choice for change detection applications. The novelty of the proposed model lies within use of fuzzy maximum likelihood classification(FMLC) as fuzzy clustering in CVA. The FMLC based CVA is implemented using diverse threshold determination algorithms such as double-window flexible pace search(DFPS), interactive trial and error(TE), and 3×3-pixel kernel window(PKW). Unlike existing CVA techniques, addition of fuzzy clustering in CVA permits each pixel to have multiple class categories and offers ease in threshold determination process. In present work, the comparative analysis has highlighted the performance of FMLC based CVA overimproved SCVA both in terms of accuracy assessment and operational complexity. Among all the examined threshold searching algorithms, FMLC based CVA using DFPS algorithm is found to be the most efficient method.     

Comparative analysis of influence factors on arable land use intensity at farm household level: A case study comparing Suyu District of Suqian City and Taixing City, Jiangsu Province, China

Using data from the farm household survey conducted in 2009,arable land use intensity(ALUI)and its influence factors at farm household level were investigated by the Tobit model.Suyu District of Suqian City and Taixing City of Jiansu Province,China were chosen as the regions for comparison.The results show that:1)On the average, the ALUI,labor intensity,yield-increasing input,and labor-saving input are 15 238.14 yuan(RMB)/ha,192 d/ha, 7233.01 yuan/ha,and 2451.32 yuan/ha in the less economically developed Suyu District,and 13 020.65 yuan/ha,181 d/ha,5871.82 yuan/ha,and 2625.97 yuan/ha in more economically developed Taixing City.The figures indicate that Suyu District has higher ALUI and labor intensity input but lower labor-saving input.2)Comparing all the influence factors,the total arable land area in available and average plot size have bigger effects on arable land intensive use;to a small degree,family′s non-farm income affects labor intensity,yield-increasing input,and labor-saving input;the yield-increasing input decreases significantly when the householder has higher education attainment;the commercialization rates of agricultural products and the planting proportion of cash crops both have unstable influence on ALUI; the share of arable land rented in has few impacts on labor intensity,yield-increasing input,and labor-saving input. 3)There are no differences found in the internal impact mechanism of influence factors on the arable land intensive use behaviors of farm households.However,there are conspicuous disparities in the impact degrees and statistical significance based on varying economic levels.4)Using the results as bases,this study proposes that the government should implement land management and agricultural policies according to local condition.And these policies should decrease land fragmentation to promote scale management of land and arable land use intensification.     

不同物源体系致密储层微观结构特征及成因分析:以陇东地区樊家川和南梁长6段为例

鄂尔多斯盆地致密油气资源丰富,其中陇东地区长6储层的发育受多物源体系的控制,成为当前研究热点,但目前对不同物源控制的砂体微观结构特征差异等缺乏深入了解,制约了该地长6储层的勘探与评价。通过铸体薄片鉴定、镜下观察、X衍射及恒速压汞分析等实验手段,开展了不同物源体系控制下的储层微观结构特征研究,对比分析了沉积微相对储层发育的影响,建立了对应的孔隙演化模型,并探讨了主要成岩作用对储层致密化的控制。结果表明：(1)喉道是控制储层渗透率的主要因素,北东部物源沉积区较西部物源沉积区的喉道发育状况更好,渗透率更高;(2)随着渗透率升高,受北东部物源控制的南梁地区半径大于0.50μm的较大喉道对渗透率的贡献明显增加;(3)沉积微相是控制不同物源体系砂体物性的重要因素,受北东部物源控制的南梁地区的砂质碎屑流和浊流砂体物性均好于受西部物源控制的樊家川地区;压实作用是造成储层致密化的主要原因,受不同物源体系储层伊利石和绿泥石含量差异的影响,北东部物源沉积区压实减孔量(19.29%)小于西部物源沉积区(22.32%)。该研究成果对不同物源体系致密油的后期评价具有指导意义。     

Historical relationships of the Mesoamerican highlands,with emphasis on tropical montane cloud forests: a temporal cladistic biogeographical analysis

The historical relationships of nine areas of endemism of the tropical montane cloud forests(TMCFs) were analysed based on a temporal cladistic biogeographical approach.Three cladistic biogeographical analyses were conducted based on 29cladograms of terrestrial taxa by partitioning them into three time-slices, namely, Miocene, Pliocene, and Pleistocene. The results showed different area relationships over time. For the Miocene and Pliocene time slices, the Isthmus of Tehuantepec acted as a geogr...