鲍苗硅藻饵料培养池中的危害性纤毛虫及其优势种群研究

应用纤毛虫的定性和定量方法,研究了规模化鲍苗硅藻饵料培养池中危害性纤毛虫的种类组成和各种类的种群变化过程,共观察到纤毛虫33种,斯坦盾纤虫Aspidiscasteini和三齿盾纤虫Aspidisca tridentata为优势种,在藻类培养过程中,危害性纤毛虫种类数量呈先增加后减少,最后时有25种,随着培养的藻类数量增加,纤毛虫数量也有所增加,在培养的第13天达到数量的最大值,其最大总密度可达21．28和21．55个.cm^-2,优势种斯坦盾纤虫和三齿盾纤虫最大密度达到5．3－7．2个.cm^-2,此外,大突口虫Condylostomamagnum,扇形游仆虫Euplotes vannus,具棘旋毛虫Dysteriaspinifera和双眉虫属Diophrys在数量上具有较高的比例,与藻类不同的是,纤毛虫的密度在达到最大后逐渐下降。     

鲍鱼幼苗培养池附板上的危害性纤毛虫及其优势类群研究

应用纤毛虫的定性和定量方法,研究了人工养殖幼鲍培养池中危害性纤毛虫的种类组成和数量变化过程及优势类群的种群动力学变化。共观察到16目、46属、68种纤毛虫,锐利Shun纤虫Aspidisca lynicaster、钟虫Vorticella sp.和双眉虫Diophrys histrix是优势种。还对幼鲍培养过程中危害性纤毛虫的密度、种类与幼苗附板上藻类密度、鲍苗密度等关系进行了分析。     

鲍养成阶段养殖环境中的浮游纤毛虫

在使用表层海水作养殖水的杂色鲍(Haliotis diversicolor)养成池,整个养成阶段共检测到浮游纤毛虫33种,其中寡毛目(Oligotrichida)的种类最多,为14种。在一个使用沙井水作养殖水的杂色鲍养成池周年研究中,共观察到浮游纤毛虫24种。2类养成池的纤毛虫优势种都是尾丝虫(Uronema sp．)、钟虫(Vorticella sp．)和具沟急游虫(Strombidium sulcatum)。虽然后者养殖用水为沙井水,但养殖密度较大,水体交换量稍低,养殖水体中有机质含量高,嗜污性纤毛虫得以大量繁殖,其密度较前者高一倍。在养殖用水取水海区——红海湾近岸表层海水中,共检测到浮游纤毛虫27种,其中砂壳亚目(Tintinnina)纤毛虫16种。受水环境中二氧化碳浓度变化影响,养成池与取水海区砂壳虫的种类组成和密度有较大区别。     

海洋贝类的病害性纤毛虫研究Ⅱ.嗜污亚目盾纤类纤毛虫

报道山东沿海贝类鳃表及外套腔中共栖的 2种盾纤类纤毛虫 ,含 1新种 :大粘叶虫(Myxophyllum magnum n.sp.)及 1种国内新记录 :双壳吸触虫 (Thigmophrya bivalviorum)。作者依据活体观察及蛋白银染色对其活体形态学、纤毛图式进行了综合描述 ,并对粘叶虫属和吸触虫属属的定义做了新的修订。     

一种纤毛虫的分类及形态研究 ——寄生于牙鲆体表溃烂组织中的指状拟舟虫

报道从牙鲆（Paralichthys olivaceus）体表溃烂组织分离的一种纤毛虫，通过活体观察、扫描电镜、蛋白银染色法、银浸法，对其形态学及纤毛图式做了研究。经鉴定，该纤毛虫为指状拟舟虫（Paralembus digitiformis Kahl，1933），隶属于寡膜纲、盾纤目、嗜污科、拟舟虫属。这是指状拟舟虫在牙鲆上兼性寄生的首次报道。     

海洋贝类的病害性纤毛虫研究Ⅲ.触毛亚目盾纤类纤毛虫

报道山东沿海贝类鳃表及外套腔中共栖的 3种触毛亚目盾纤类纤毛虫 :厚鱼钩虫(Ancistrum crassum)、日本鱼钩虫 (Ancistrum jap onicum)及亚桶形后口虫 (Boveriasubcylindrica)。文中依据活体观察、蛋白银及银浸法染色对其活体形态学、纤毛图式及银线结构进行了综合描述 ,对采自 4种不同宿主的厚鱼钩虫种群进行了比较研究。该文系后口虫属纤毛虫在我国的首次报道。     

海水养殖水体中危害性纤毛虫原生动物研究Ⅰ.盾纤目习见类群的口器结构及鉴别特征

对发现自我国北方海区养殖环境中危害性盾纤目纤毛虫的常见类群 (16属 )之口器结构、一般形态学与鉴别特征以及属种模式做了概要性介绍。同时涉及了生活类型、已知种的分布范围、纤毛图式水平的示差鉴别及属级定义并借助模式图例为该类广泛分布而又不易识别的病害纤毛虫类群提供了一份简明的病原鉴定资料     

热带西太平洋浮游纤毛虫的垂直分布

于2014年12月至2015年1月在热带西太平洋沿台湾南部到雅浦海山一个断面和雅浦海山海区研究了浮游纤毛虫丰度和生物量的垂直分布。浮游纤毛虫丰度变化范围为0—635ind./L,生物量范围为0—1.53μg C/L,丰度和生物量高值分布于200m以浅,其中,砂壳纤毛虫丰度为0—45ind./L,占总纤毛虫丰度的比例在0—14.62%之间。浮游纤毛虫丰度垂直分布均呈现"双峰型"模式:在表层和叶绿素极大值层(DCM)出现高值。共鉴定出砂壳纤毛虫33属76种。雅浦海山海区优势种为纤弱细瓮虫、卢氏真铃虫、管状真铃虫、膨大波膜虫,西太平洋海区优势种为纤弱细瓮虫、海勒斯真铃虫、酒杯类管虫、尖锐号角虫。有些种类分布在100m以浅,有的种类分布在100m以深,说明砂壳纤毛虫在100m水深左右种类发生变化。     

海洋纤毛虫巨大拟阿脑虫的实验生态学研究:初始密度及食物对其种群生长的影响

采用实验生态学方法，就不同初始种群密度及食物密度等因素对海洋纤毛虫巨大拟阿脑虫（隶属纤毛门，盾纤目）的种群自然增长率（r)及世代时间（G）的影响进行了探讨。结果表明，初始种群密度与r值呈负相关，与G则呈正相关，初始密度为1ind/ml时，r值达最大值2．61/d，G值则为最小值为0．29d;r值随食物密度的增大而增大，当食物密度为2．8E＋08ind/ml时，r值达最大值为2．07。同时讨论了种群自然增长率和世代时间之计算方法在实验中的应用。     

鲍人工养成阶段养殖环境中的周丛纤毛虫

应用定性和定量方法,研究了人工养成阶段杂色鲍Haliotis diversicolor外壳的周丛纤毛虫的种类组成和优势类群演变。共观察到3纲9目35属58种纤毛虫,缘毛类Peritrichida的种类最多,有23种。在养成阶段的早期,周丛纤毛虫的优势类群为钟虫类;在养成阶段的中、后期,周丛纤毛虫的优势类群演变为聚缩虫类,其中交替聚缩虫Zoothamnium alternans占绝对优势;文中还对各种环境因子对养殖环境中的周丛纤毛虫的影响进行了分析。     

Microbial Biomass and Organic Nutrients in the Deep-Sea Sediments of the Central Indian Ocean Basin

In order to assess the impact of deep-sea mining on the in situ benthic life, we measured the microbial standing stock and concentration of organic nutrients in the deep-sea sediments of the Central Indian Ocean Basin in the Indian pioneer area. Sediments were collected using box core and grab samples during September 1996. The total bacterial numbers ranged from 10 10 -10 11 cells per g -1 dry weight sediment. There was a marginal decrease in the number of bacteria from surface to 30 cm depth, though the subsurface section registered a higher number than did the surface. The highest numbers were encountered at depths of 4-8 cm. The retrievable number of bacteria were two orders less in comparison with the direct total counts of bacteria. An almost homogeneous distribution of bacteria, total organic carbon, living biomass, and lipids throughout the depth of cores indicates active microbial and benthic processes in the deep sea sediments. On the other hand, a uniform distribution of total counts of bacteria, carbohydrates, and total organic carbon in all the cores indicates their stable nature and suggests that they can serve as useful parameters for long-term monitoring of the area after the benthic disturbance. Further studies on temporal variability in this region would not only verify the observed norms of distribution of these variables but would also help to understand restabilization processes after the simulated benthic disturbance.     

海上大直径钢管桩打桩过程中桩周土强度弱化研究

海上大直径钢管桩打桩过程中,桩周土体受到强烈扰动而发生强度弱化,掌握桩周土体强度弱化规律对于准确预测打桩过程、保证工程安全具有重要意义。为研究土体强度弱化规律,开展了环剪试验模拟打桩对桩周土体的扰动,测试土体强度随剪切速率的变化规律,建立了描述土体强度弱化规律的拟合公式,引入到打桩分析软件中。研究结果表明：土体的强度折减程度不仅与土体本身的性质有关还受到土体的埋深和剪切速率的影响,埋深越深土体强度折减程度越低,剪切速率越高土体强度折减越高,在打桩分析中可采用这里推荐的线性折减方法来模拟不同深度处土体强度的折减规律。     

Geoacoustic Inversion via Genetic Algorithm and Its Application to Manganese Sediment Identification

An acoustic inversion method using a wide-band signal and two near field receivers is proposed and applied to multiple layered seabed models including a manganese sediment. The inversion problem can be formulated into a probabilistic model comprised of signals, a forward model, and additive noise. The forward model simulates wide-band signals, such as chirp signals, and is chosen to be the source-waveletconvolution plane wave modeling method. The wavelet matching technique, using weighted least-squares fitting, estimates the sediment sound-speed and thickness on which determination of the possible numerical ranges for a priori uniform distribution is based. The genetic algorithm is applied to a global optimization problem to find a maximum a posteriori solution for determined a priori search space. Here the object function is defined by an L 2 norm of the difference between measured and modeled signals. Not only the marginal pdf but also its statistics are calculated by numerical evaluation of integrals using the samples selected during importance sampling process of the genetic algorithm.     

Phylogenetic diversity and phenotypic characterization of cultivable bacterioplankton isolated from polar oceans

A set of 27 marine planktonic bacteria isolated from the polar regions was characterized by 16S rDNA sequencing and physiological and biochemical testing. More than half of these bacteria were positive for caseinase, gelatinase and 13-glucosidase, and could utilize glucose, maltose or malic acid as carbon source for cell growth. Twelve isolates expressed nitrate reduction activities. Except for one antarctic isolate BSwlO175 belonging to Actinobacteria phylum, these isolates were classified as γ-Proteobacteria, suggesting that γ-Proteobacteria dominated in cultivable marine bacterioplankton at both poles. Genus Pseudoalteromonas was the predominant group in the Chukchi Sea and the Bering Sea, and genus ShewaneUa dominated in cultivable bacterioplankton in the Prydz Bay. With sequence similarities above 97%, genus Psychrobacter was found at both poles. These 27 isolates were psychrotolerant, and significant 16S rDNA sequence similarities were found not only between arctic and antarctic marine bacteria （ 〉 99% ）, but also between polar marine bacteria and bacteria from other aquatic environments （ ≥ 98.8% ）, including temperate ocean, deep sea, pond and lake, suggesting that in the polar oceans less temperature-sensitive bacteria may be cosmopolitan and have a bipolar, even global, distribution at the species level.     

Deep-Sea Sediment Compression Curves: Some Controlling Factors, Spurious Overconsolidation, Predictions, and Geophysical Reproduction

Oedometer tests have been carried out on 70 undisturbed surficial clays (at approximately 250 mm below the mudline), mostly collected by free-fall corers from sites widely scattered throughout the deep-sea North Atlantic. Acoustic measurements were also made, initially on contiguous samples and ultimately on the same sample using a geophysically instrumented oedometer which also collected electrical resistivity data. Apart from those quiescent areas below the carbonate compensation depth, such as north of the West Indies where very fine clays exist, most of the samples are silty clays whose geotechnical-geophysical properties are dependent on the type of clay minerals present (and their ability to take in moisture), the sand-size fraction, and the quantity of carbonate present. Thus the pure clays have high compressibilities which decrease on the addition of coarse particles, while the converse is true for the acoustic parameters, these increasing with the sand fraction. Using the notion of the intrinsic compression line for all samples, and comparison to it of the measured compression curves, it is clear that, contrary to some previously held ideas, most deep-sea clays are normally consolidated; the addition of carbonate has the effect of creating an open, stronger sediment skeleton. Interestingly, where information is available, the variation with depth of a sample's acoustic velocity follows the void ratio pressure relationship of the compression curve. This allows the construction of an in-situ sediment compression curve using the in-situ geophysical observations.     

INFORMATION FOR AUTHORS

<正>Acta Oceanologica Sinica(AOS)is a comprehensive academic journal edited by the Editorial Committee of Acta Oceanologica Sinica and is designed to provide a forum for important research papers of the marine scientific community which reflect the information on a worldwide basis.The journal publishes scholarly papers on marine science and technology,including physics,chemistry,biology,     

Evaluation of the Risk of Marine Slope Instability: A Pseudo-3D Approach for Application to Large Areas

This article presents a methodology developed to evaluate the instability of submarine slopes that extend over a large area. Special attention was paid to (1) the complex geometry (bathymetry) and the expanse of the slope, (2) the heterogeneity of the sediment, and (3) the distribution of the pore pressure. The safety factor was considered as a spatially varying quantity. The General Formulation (GLE, Fredlund and Krahn 1977), which fully satisfies equilibrium conditions, was used for evaluating the stability of the marine slope. The submarine slope failure, which occurred on 16 October 1979 during the construction of the new Nice airport, was studied in order to test the developed model. Geotechnical parameters were taken from experimental tests carried out by IFREMER on 19 cores extracted at different depths (from 27 m to 1300 m) (Cochonat, Bourillet, and Savoye, 1993; Mulder et al., 1994). Many scenarios were proposed in order to explain the cause of the Nice slope failure (Habib, 1994). In this article, two of those scenarios were tested. Simulation results are presented and discussed.