Sporormiella as a proxy for non-mammalian herbivores in island ecosystems

Spores of the dung-fungi Sporormiella are routinely used as a proxy for past megaherbivore biomass and to pinpoint the timing of extinctions. Further ecological insight can also be gained into the impacts that followed initial human arrival in a region through correlation of spore abundance with other proxies (e.g. pollen, charcoal). Currently, the use of Sporormiella as a palaeoecological proxy has been restricted to landmasses where large-herbivore guilds are dominated by mammals. Here, we use New Zealand as a case study to show that the method can also be applied effectively to islands dominated by large avian herbivores. We examine 44 dung samples from 7 localities to show that Sporormiella spores were widely distributed in the dung of endemic avian herbivores (South Island takahe (Porphyrio hochstetteri), kakapo (Strigops habroptilus), and several species of extinct moa, identified by ancient DNA analysis). In addition, we show that Sporormiella spores in a forest soil core from the Murchison Mountains, South Island, accurately trace the post-settlement decline of native avian herbivores, and combined with high-resolution radiocarbon dating reveal severely reduced local herbivore populations by the late 17th Century AD. The spores also trace the subsequent spread of Red deer (Cervus elaphus) introduced to the area in the early 20th Century AD. Our results suggest Sporormiella spores may provide a useful new tool for examining extinctions on numerous islands where terrestrial herbivore guilds were dominated by birds or reptiles. Our findings also highlight the need to consider entire herbivore communities (including birds and reptiles) when examining Late Pleistocene continental Sporormiella records, where the focus has often been tracing the decline of populations of large mammals.     

A test of Sporormiella representation as a predictor of megaherbivore presence and abundance

Spores of the dung fungus Sporormiella have been suggested to indicate the presence, perhaps also the abundance of past megaherbivore populations. Nonetheless, basic studies demonstrating a correlation between Sporormiella concentration in lacustrine sediments and modern herbivore abundance are lacking. This study of Sporormiella representation in grazed and ungrazed landscapes provides supporting evidence for the application of Sporormiella as an indicator of megaherbivore presence and abundance in ancient landscapes. However, Sporormiella representation is spatially sensitive to the distance from the dung source. In lakes where Sporomiella are abundant in shoreline sediments, they decline sharply with increasing distance from the lake edge. Although this study provides supporting evidence for the application of Sporormiella as a proxy for herbivore presence and abundance, independent proxies should be applied in conjunction with Sporormiella to control for changes in lake size.     

What does the occurrence of Sporormiella (Preussia) spores mean in Australian fossil sequences?

Understanding the loss of the final few species of Australian megafauna is beset by a paucity of data on human arrival, well‐provenanced megafauna, human/megafauna population range and distribution (coexistence and interaction), and the range, scale and impact of environmental changes spanning the human–megafauna period. To overcome these shortcomings, the occurrence and decline of coprophilous fungal spores of Sporormiella in sediments have been used as a proxy for extinct megaherbivores. The Sporormiella evidence is presented as the key indicator of extinction timing and these reports are often from locations where there is no known archaeological record or megafauna remains. However, interpreting fungal spore occurrence is not straightforward, as demonstrated by studies investigating taphonomy, taxonomy and the types of animal dung where Sporormiella occurs. No detailed studies on these problems exist for Australia and no evidence supporting the use of Sporormiella as a valid proxy has been reported. Here we examine the occurrence of Sporormiella spores from Cuddie Springs in south‐eastern Australia. Despite a well‐preserved suite of megafauna fossils, Sporormiella occurrence is sporadic and frequencies are low. We conclude that using Sporormiella alone as an indicator for the presence of megafauna is premature for the Australian context.     

Wetland soil moisture complicates the use of Sporormiella to trace past herbivore populations

Spores of the dung‐fungi Sporormiella provide a proxy for assessing past changes in herbivore abundance, and for temporally linking these with shifts in vegetation communities (pollen) and fire frequency (charcoal). Although the proxy is now widely used, potential influences of local environmental conditions are poorly understood. Here we assess the reliability of Sporormiella as a proxy of herbivores by presenting five new spore records from the South Island of New Zealand; a land where the herbivore extinction/introduction chronology is well established, and where extinctions occurred independently of major climate change. We show that Sporormiella spores from relatively dry soils provide a good proxy for herbivore extinctions and introductions. However, spore abundances from saturated soil sites appear to fluctuate in close correlation with changes in local hydrology (represented by wetland herb pollen, testate amoebae and moisture sensitive mosses). Our results suggest Sporormiella records derived from wetlands require cautious interpretation, and that proxies for hydrological conditions should also be considered when interpreting such records. Copyright © 2011 John Wiley & Sons, Ltd.     

Late Pleistocene climate change,nutrient cycling,and the megafaunal extinctions in North America

This study proposes an ecological mechanism for the terminal Pleistocene population collapse and subsequent extinction of North American megafauna. Observations of modern ecosystems indicate that feedback mechanisms between plant nutrient content, nitrogen cycling, and herbivore–plant interactions can vary between a nutrient accelerating mode favoring increased herbivore biomass and a nutrient decelerating mode characterized by reduced herbivore biomass. These alternate modes are determined largely by plant nitrogen content. Plant nitrogen content is known to be influenced by atmospheric CO₂ concentrations, temperature, and precipitation. It is argued that Lateglacial climate change, particularly increases in atmospheric CO₂, shifted herbivore–ecosystem dynamics from a nutrient accelerating mode to a nutrient decelerating mode at the end of the Pleistocene, leading to reduced megafaunal population densities. An examination of Sporormiella records – a proxy for megaherbivore biomass – indicates that megafaunal populations collapsed first in the east and later in the west, possibly reflecting regional differences in precipitation or vegetation structure. The fortuitous intersection of the climatically driven nitrogen sink, followed by any one or combination of subsequent anthropogenic, environmental, or extra-terrestrial mechanisms could explain why extinctions took place at the end of the Pleistocene rather than during previous glacial–interglacial cycles.     

Four thousand years of environmental change and human activity in the Cochabamba Basin, Bolivia

The Cochabamba Basin (Bolivia) is on the ancient road network connecting Andean and lowland areas. Little is known about the longevity of this trade route or how people responded to past environmental changes. The eastern end of the Cochabamba valley system constricts at the Vacas Lake District, constraining the road network and providing an ideal location in which to examine past human-environmental interactions. Multi-proxy analysis of sediment from Lake Challacaba has allowed a c. 4000 year environmental history to be reconstructed. Fluctuations in drought tolerant pollen taxa and calcium carbonate indicate two periods of reduced moisture availability (c. 4000-3370 and c. 2190-1020 cal yr BP) compared to adjacent wetter episodes (c. 3370-2190 and c. 1020 cal yr BP-present). The moisture fluctuations broadly correlate to El Niño/Southern Oscillation variations reported elsewhere. High charcoal abundance from c. 4000 to 2000 yr ago indicates continuous use of the ancient road network. A decline in charcoal and an increase in dung fungus (Sporormiella) c. 1340-1210 cal yr BP, suggests that cultural changes were a major factor in shaping the modern landscape. Despite undisputable impacts of human populations on the Polylepis woodlands today, we see no evidence of woodland clearance in the Challacaba record.     

Late quaternary insects of Rancho La Brea and McKittrick,California

Asphalt-impregnated sediments at Rancho La Brea (Los Angeles County) and McKittrick (Kern County) in California provide a rich Quaternary insect record. Ages of various sites at Rancho La Brea range from more than 40,000 ¹⁴C yr B.P. to modern. McKittrick insects studied by W. D. Pierce are not contemporaneous with the late Pleistocene vertebrate fauna, but are only about 7000 ¹⁴C yr old. The major paleoecological groupings are: (1) ground dwellers, (2) aquatics. (3) scavengers, and (4) miscellaneous. Contrary to conclusions of earlier authors, most specimens represent modern species. Only two apparent terminal Pleistocene extinctions are recognized. both dung beetles (Scarabaeidae).     

Pleistocene oceanographie changes indicated by deep sea benthic foraminifera in the northern Indian Ocean

An attempt has been made to understand the Pleistocene bottom water history in response to the paleoclimatic changes in the northern Indian Ocean employing quantitative analyses of deep sea benthic foraminifera at the DSDP sites 219 and 238. Among the 150 benthic foraminifera recorded a few species show dominance with changing percent frequencies during most of the sequence. The dominant benthic foraminiferal assemblages suggest that most of the Pleistocene bottom waters at site 219 and Early Pleistocene bottom waters at site 238 are of North Indian Deep Water (NIDW) origin. However, Late Pleistocene assemblage at site 238 appears to be closely associated with a water mass intermediate between North Indian Deep Water (NIDW) and Antarctic Bottom Water (AABW). Uvigerina proboscidea is the most dominant benthic foraminiferal species present during the Pleistocene at both the sites. A marked increase in the relative abundance ofU. proboscidea along with less diverse and equitable fauna during Early Pleistocene suggests a relative cooling, an intensified oceanic circulation and upwelling of nutrient rich bottom waters resulting in high surface productivity. At the same time, low sediment accumulation rate during Early Pleistocene reveals increased winnowing of the sediments possibly due to more corrosive and cold bottom waters. The Late Pleistocene in general, is marked by relatively warm and stable bottom waters as reflected by low abundance ofU. proboscidea and more diverse and equitable benthic fauna. The lower depth range for the occurrence ofBulimina aculeate in the Indian Ocean is around 2300 m, similar to that of many other areas.B. aculeata also shows marked increase in its abundance near the Pliocene/Pleistocene boundary while a sudden decrease in the relative abundance ofStilostomella lepidula occurs close to the Early/Late Pleistocene boundary.     

Southern dispersal and Palaeoecological implications of woolly rhinoceros (Coelodonta antiquitatis): review of the Iberian occurrences

Cold-adapted large mammal populations spread southward during the coldest and driest phases of the Late Pleistocene reaching the Iberian Peninsula. Presence of woolly rhinoceros (Coelodonta antiquitatis) can be identified from 23 Iberian sites, which is compiled and analyzed herein, and the fossil specimens from seven of these sites are described here for first time.Morphological and biometrical analyses demonstrate that the Iberian woolly rhinoceros did not significantly differ from individuals of other European populations, but represent the westernmost part of a continuous Eurasian belt of distribution.The first presence of woolly rhino in the Iberian Peninsula has been identified during the late Middle Pleistocene and early Late Pleistocene. However, the highest abundance of this species is recorded during MIS 3 and 2. The latest Iberian occurrences can be dated around 20 ka BP. The presence of woolly rhinoceros in the Iberian Peninsula correlates with periods of extreme dry and cold climatic conditions documented in Iberian terrestrial and marine sediment sequences.From a palaeobiogeographic point of view, the maximum southern spread of C. antiquitatis on the Iberian Peninsula was registered during the late Middle Pleistocene or early Late Pleistocene, reaching the latitude of Madrid (about 40°N). Subsequently, during MIS 3 and 2, all Iberian finds were restricted to the Northern regions of Iberia (Cantabrian area and Catalonia). The southern expansion of C. antiquitatis during the Late Pleistocene in the Iberian Peninsula reached similar latitudes to other Eurasian regions.The ecological composition of fossil assemblages with presence of woolly rhinoceros was statistically analyzed. Results show that temperate ungulate species are predominant at Iberian assemblages, resulting in a particular mixture of temperate and cold elements different of the typical Eurasian cold-adapted faunal associations. This particular situation suggests two possible explanations: a) Eventual migrations during the coldest time spans, resulting in a mixing of cold and temperate faunas, instead a faunal replacing; b) Persistence of woolly rhinoceros populations in the Iberian Peninsula during interglacial episodes confined at cryptic southern refugia.     

Dung, diet, and the paleoenvironment of the extinct shrub-ox (Euceratherium collinum) on the Colorado Plateau, USA

Fossil remains of Euceratherium collinum (extinct shrub-ox) have been found throughout North America, including the Grand Canyon. Recent finds from the Escalante River Basin in southern Utah further extend the animal's range into the heart of the Colorado Plateau. E. collinum teeth and a metapodial condyle (foot bone) have been recovered in association with large distinctively shaped dung pellets, a morphology similar to a ‘Hershey's Kiss’ (HK), from a late Pleistocene dung layer in Bechan Cave. HK dung pellets have also been recovered from other alcoves in the Escalante River Basin including Willow and Fortymile canyons. Detailed analyses of the HK pellets confirmed them to be E. collinum and indicate a browser-type diet dominated (> 95%) by trees and shrubs: Artemisia tridentata (big sagebrush), Acacia sp. (acacia), Quercus (oak), and Chrysothamnus (rabbit brush). The retrieval of spring and fall pollen suggests E. collinum was a year-round resident in the Escalante River Basin.     

Characterisation of organic matter and microbial communities in contrasting arsenic-rich Holocene and arsenic-poor Pleistocene aquifers,Red River Delta,Vietnam

High concentrations of geogenic As in the groundwaters of south and SE Asia, which are used as drinking waters, are causing severe health impacts to the exposed human populations. It is widely accepted that As mobilisation from sediments into these shallow reducing groundwaters requires active metal-reducing microbes and electron donors such as organic matter (OM). Although OM in such Holocene aquifers has been characterised, there is a dearth of data on Pleistocene aquifers from the same areas. Reported here are preliminary studies of OM and microbial communities present in two aquifers, one of Pleistocene and one of Holocene age, with contrasting concentrations of As (viz. Pleistocene: low As <10 μg/L; Holocene: high As up to 600 μg/L) from Van Phuc village in the Red River Delta, Vietnam. Results revealed OM inputs from multiple sources, including potential contributions from naturally occurring petroleum seeping into the shallow aquifer sediments from deeper thermally mature source rocks. Although concentrations vary, no noticeable systematic differences in biomarker distribution patterns within the OM were observed between the two sites. Microbial analyses did not show a presence of microbial communities previously associated with As mobilisation. All clone libraries were dominated by α-, β-, and γ-Proteobacteria not known to be able to reduce Fe(III) or sorbed As(V). Furthermore, representatives of the Fe(III)-reducing genus Geobacter could only be detected at very low abundance by PCR, using highly selective 16S rRNA gene primers, supporting the hypothesis that metal reduction is not a dominant in situ process in these sediments. No correlation between As concentration in groundwater and OM composition nor microbial community in the host sediments was found. This suggests that either (i) As is not being significantly mobilised in situ in these sediments, instead As appears to be mobilised elsewhere and transported by groundwater flow to the sites or (ii) sorption/desorption processes, as implicated by geochemical data from the cores, play a critical role in controlling As concentrations at these sites.     

加利福尼亚洋流北区早更新世以来似太平洋年代际振荡的微体化石记录

加利福尼亚洋流系统(CCS)是东北太平洋一股十分重要的边界流,在全球海气交换过程中具有重要地位。CCS的海洋-大气变化尤其是涌升流的强弱变化,在年代和多年代时间尺度上的变化主要受太平洋年代际震荡(PDO,20~30年周期)的控制。为了解CCS北区早更新世以来涌升流强弱变化规律及其主要控制机理,本文分析了国际大洋钻探1020B(CCS北区南部41°N)和1245B(北部44°N)钻孔钙质超微化石和硅藻化石等记录。结果显示,多半时间段的记录具有冰期和间冰期旋回的变化;其中超微化石的高含量见于间冰期,而硅藻高含量对应于冰期。这两类化石在沉积物中的含量变化显示出时间尺度长约0.5 Ma的3个阶段:早更新世(1.5~1.1 Ma)两个孔以超微化石含量高为特征;中更新世(1.1~0.65 Ma)两孔中的超微化石和硅藻化石含量相对较高,但它们在两个孔的变化趋势不同;晚更新世(0.65 Ma)以来,南部1020B孔超微化石含量高且出现频率高于硅藻化石,而北部1245B孔以硅藻化石含量较高且持久出现为特征。本文提出了该区涌升流受长周期“似太平洋年代际振荡模式”和“似北太平洋环流振荡模式”控制的假设。早更新世(1.5~1.1 Ma)主要受到持续的似PDO正位相 (似+PDO,表层海水温暖)的控制,涌升流弱,海水中碳酸盐离子丰富,利于颗石藻骨骼钙化形成颗石和颗石产量高,沉积物中超微化石含量高。中更新世过渡期(MPT,1.1~0.65 Ma)受到似PDO负位相(似-PDO,海水表层水体冷、涌升流强、海水pH值低,硅藻生产力高,沉积物中硅藻化石含量高)与似+PDO的交替影响。晚更新世(0.65 Ma)以来,南部1020站位受到似-PDO/似-NPGO?的交替控制,但似-NPGO?的强度和出现频率均大于似-PDO,因此超微化石含量和出现时期比硅藻多;北部1245站位则仅受似-PDO的持续性控制,涌升流较强且持续时间长、沉积物中硅藻化石几乎持续出现且含量较高。MPT时期以硅藻化石含量在1.1 Ma突然增加(南部增加3倍,北部5倍)为起始点,以0.65 Ma时超微化石含量突变(南部增加3倍,北部降3倍)为终止点。本文认为MPT起止点化石含量的突变,是古海洋和气候环境突变的结果,也意味着长时间尺度的似PDO的正负位相之间的转换变化可能是突变过程。MPT时期CCS北部区域内出现了南部和北部站位硅质和钙质两类化石含量变化趋势相反的记录,指示着这一时期该区的古海洋和气候条件出现了地理上的南北分异,并且自0.65 Ma以来南北两个站位控制模式不同。本文的这些记录为CCS区现代海洋-大气过程和生物地理分区大致以40°N为界的研究提供了早更新世以来的历史记录。     

Carbon and oxygen stable isotope compositions of late Pleistocene mammal teeth from dolines of Ajoie (Northwestern Switzerland)

Fossils of megaherbivores from eight late Pleistocene ¹⁴C- and OSL-dated doline infillings of Ajoie (NW Switzerland) were discovered along the Transjurane highway in the Swiss Jura. Carbon and oxygen analyses of enamel were performed on forty-six teeth of large mammals (Equus germanicus, Mammuthus primigenius, Coelodonta antiquitatis, and Bison priscus), coming from one doline in Boncourt (~ 80 ka, marine oxygen isotope stage MIS5a) and seven in Courtedoux (51–27 ka, late MIS3), in order to reconstruct the paleoclimatic and paleoenvironmental conditions of the region. Similar enamel δ¹³C values for both periods, ranging from − 14.5 to − 9.2‰, indicate that the megaherbivores lived in a C₃ plant-dominated environment. Enamel δ¹⁸O_PO4 values range from 10.9 to 16.3‰ with a mean of 13.5 ± 1.0‰ (n = 46). Mean air temperatures (MATs) were inferred using species-specific δ¹⁸O_PO4–δ¹⁸O_H2O-calibrations for modern mammals and a present-day precipitation δ¹⁸O_H2O-MAT relation for Switzerland. Similar average MATs of 6.6 ± 3.6°C for the deposits dated to ~ 80 ka and 6.5 ± 3.3°C for those dated to the interval 51–27 ka were estimated. This suggests that these mammals in the Ajoie area lived in mild periods of the late Pleistocene with MATs only about 2.5°C lower than modern-day temperatures.     

Activity,Abundance, and Diversity of Nitrifying Archaea and Denitrifying Bacteria in Sediments of a Subtropical Estuary: Bahía del Tóbari,Mexico

Fixed nitrogen (N) removal from estuaries via coupled nitrification–denitrification plays a significant role in the global N cycle and the biogeochemistry of individual estuaries. Much of our understanding of these processes is drawn from temperate estuaries, yet tropical and subtropical estuaries may respond differently to N inputs. I tested the hypothesis that nitrification is limited within subtropical estuaries by comparing nitrification and denitrification potentials, and the abundance of archaeal ammonia monooxygenase (amoA) and bacterial nitrite reductase (nirS) genes, across five sites in Bahía del Tóbari, Mexico. Sampling was conducted when agricultural runoff supplied substantial quantities of N (ca. 20–80 μM ammonium), yet nitrification was detected at a single site. Denitrification was measured at four sites, and three displayed nitrate uptake rather than net nitrification—indicating a N sink within these sediments. Bacterial nirS genes uniformly outnumbered archaeal amoA genes (3- to 49-fold) and were more abundant in the northern part of the estuary. Patterns of community similarity among different sites were also different for nirS and archaeal amoA: similarities between sites based on nirS were often greater than for amoA, and sites were more rarely statistically different from each other. While amoA abundance was inversely related to temperature, neither amoA nor nirS was correlated with nitrification or denitrification potentials. My results are broadly consistent with known and proposed patterns of nitrification and denitrification in subtropical estuarine sediments, including the idea that nitrification is limited within subtropical estuarine sediments.     

Pleistocene landscapes in central Iberia inferred from pollen analysis of hyena coprolites

New pollen data from hyena coprolites from central Spain are presented. The fossil faecal material has been recovered from two karstic systems in different localities, Villacastín and Los Torrejones, which are both around 1000 m a.s.l. The combined findings of bone remains and coprolites in both locations suggest the following chronology: late Middle Pleistocene for Villacastín and early Upper Pleistocene for Los Torrejones. The environments inferred from pollen are broadly in keeping with evidence from associated vertebrate fossil remains, and include a shifting mosaic of open and wooded habitats with abundant pine and juniper species, steppe‐grassland areas with composites and chenopods, and enclaves with mixed oak forests. However, Los Torrejones appears to have been less forested than Villacastín. The abundance of oaks in Villacastín may imply the presence of refugia within an interconnected network of several enclaves during the glacial stages in the Upper Pleistocene. A possible explanation for the patchiness of the landscape may be in the role of herbivores, although the long distances and variety of habitats that hyenas had to roam through could be another explanation for the heterogeneous pollen contents in their dung. Copyright © 2006 John Wiley & Sons, Ltd.     

Pre-glacial and interglacial pollen records over the last 3 Ma from northwest Canada: Why do Holocene forests differ from those of previous interglaciations?

We synthesize pollen spectra from eleven dated stratigraphic sections from central and northern Yukon. Palaeomagnetic and tephra dating indicates the earliest assemblages, representing closed canopy Pinus and Picea forest, are middle-late Pliocene age. More open forest conditions, indicated by increased Poaceae and with evidence of permafrost, are dated at ca 3 Ma. While Pinus pollen is abundant at 3 Ma, it is reduced in records after 2.6 Ma, and subsequent Pleistocene interglacial forest records are repeatedly dominated by Picea, along with Alnus and small but significant amounts of Abies. Surface sample comparisons indicate that Abies was more widespread and abundant in past interglaciations than at present and that Middle-Pleistocene Picea–Abies forest grew in the northern Yukon Porcupine Basin, 500 km beyond modern Abies limits. In contrast, Pinus, which occurs today in southern and central Yukon, was not a significant component of these Pleistocene interglacial forests. Late-Holocene pollen assemblages with rare Abies and high Pinus are the most distinct in the past 2.6 Ma. Possible factors driving Holocene difference are paleoclimate, paludification, changes in megafaunal herbivory and an unusual fire regime. Anthropogenic burning is a factor unique to the Holocene, and if it is shown to be important in this case, it would challenge our notion of what constitutes boreal wilderness.     

Ruffordia goeppertii (Schizaeales,Anemiaceae) – A common fern from the Lower Cretaceous Crato Formation of northeast Brazil

Extraordinarily well preserved fern macrofossils of Ruffordia goeppertii (Dunker) Seward (Schizaeales, Anemiaceae) are described from the Lower Cretaceous (late Aptian) Nova Olinda Member of the Crato Formation, northeast Brazil. The identification is based on the morphology of macrofossils and in situ spores, taken from organically preserved material. This extinct, relatively small fern exhibits dimorphic fronds with sterile and fertile pinnules and schizaeoid sporangia, including cicatricose spores. The growth form with clearly differentiated sterile and fertile pinnae may be interpreted as an ancestral state in the phylogeny of the extant genus Anemia. The abundance of this fern reflects its role as ground cover in at least partly (dry) sunny areas, possibly in fern savannah-like habitats, with adaptations to survive drought stress. Finds of Ruffordia in northern Gondwana extend the wide palaeogeographic range of this taxon.     

Quaternary records of the dire wolf, Canis dirus, in North and South America

The dire wolf was an important large, late Pleistocene predator in North and South America, well adapted to preying on megaherbivores. Geographically widespread, Canis dirus is reported from 136 localities in North America from Alberta, Canada, southward and from three localities in South America (Muaco, Venezuela; Talara, Peru; and Tarija, Bolivia). The species lived in a variety of environments, from forested mountains to open grasslands and plains ranging in elevation from sea level to 2255 m (7400 feet). Canis dirus is assigned to the Rancholabrean land mammal age of North America and the Lujanian land mammal age of South America and was among the many large carnivores and megaherbivores that became extinct in North and South America near the end of the Pleistocene Epoch.     

The Sima de los Huesos (Burgos,northern Spain): palaeoenvironment and habitats of Homo heidelbergensis during the Middle Pleistocene

Interpreting how environmental dynamics respond to global climate change and how this has affected human evolution and dispersal is an on-going topic of debate. During the early Middle Pleistocene (～0.6–0.4 Ma), as compared to earlier, environmental conditions were relatively more stable, with longer climatic cycles alternating between open and forested landscapes. During this interval, humans spread successfully providing an important number of fossil sites where fossils or tools are reported. The Atapuerca-Sima de los Huesos (Burgos, northern Spain) site (Atapuerca-SH) is one of the earliest localities with hominin evidence in the European Middle Pleistocene, with the most important accumulation of Homo heidelbergensis so far. We have analyzed the abundant faunal record from Sima de los Huesos, which is mainly comprised of carnivores, in order to approach an interpretation of the palaeoenvironmental circumstances where these hominids inhabited within the Sierra. Other sites from Sierra de Atapuerca referred to the same Faunal Unit (FU 6), are roughly contemporaneous, and include important ungulates, which are here analyzed with Atapuerca-SH. Additional information provided by isotopic analysis helps elucidate the ancient ecology of taxa present in Sima de los Huesos allowing for an accurate portrayal of the setting in which humans lived. The timing of the spread of Homo heidelbergensis is dominated by a relative climatic and environmental stability and points to a landscape dominated by savannah-like open woodland.