On a new species ofPavlova (Prymnesiophyceae) from China

Pavlova viridis sp. nov. is described on the basis of light and electron microscope observations. The material was collected from the coast of haiyang county of Shandong, China. It is characterized by 1) yellowish green to green chloroplast, 2) rudimentary short flagellum 0.3 μm long and hook-shaped, 3) long flagellum with small spherical knob-scales, 4) absence of pyrenoid and stigma, and 5) marine habitat. This new species andP. salina seem to be most closely related to each other but their colour, the insertion of their three appendages, the shape of their knob scales, and their cell periplasts are quite different from one another. Contribution No. 1879 from the Institute of Oceanology, Academia Sinica     

Development of a Fast ELISA for the Specific Detection of both Leucomalachite Green and Malachite Green

Malachite green(MG), a dye, is an antifungal agent that has been used to treat and prevent fish diseases. It is metabolized into reduced leucomalachite green forms(LMG) that may reside in fish muscles for a long period, thus being harmful to human health. The aim of this study was to develop a competitive and direct enzyme-linked immunosorbent assay(ELISA) to detect MG and LMG specifically. The monoclonal antibody(m Ab) to LMG was generated using a hybridoma technique. The obtained m Ab showed good cross-reactivity(CR) to malachite green(MG), but not to crystal violet(CV) and Brilliant Green(BG). The m Ab was used to develop a fast detecting ELISA of MG and LMG in fish. By introducing the conjugation LMG-HRP, the detection capability was 0.37 ng m L-1 for MG and LMG. The mean recovery from spiked grass carp tissues ranged from 76.2% to 82.9% and the coefficients of variation varied between 1.8% and 7.5%. The stable and efficient monoclonal cell line obtained is a sustainable source of sensitive and specific antibody to MG and LMG.     

Spermiogenesis in the yellowfin porgy (Sparus latus Houttuyn), with emphasis on the associated mitochondrion

Transmission electron microscopy of the yellowfin porgy (Sparus latus Houttuyn) spermatozoa ultrastructure showed the spermatozoon as a primitive type made up of the acrosomeless head, the flagellum, and the midpiece, at the periphery of which was a relatively big mitochondrion with more complex structure. It was found that during spermiogenesis, only one relatively big mitochondrion occurred in both the spermatid and the spermatozoon. This is different from other teleost fishes. During spemiogenesis, the mitochondria number is one, and morphology did not change. All these are different from those of other fishes.     

大连市城区绿地时空特征的热环境效应研究

本文选择大连市沙河口和西岗区为研究区,以Landsat ETM、SPOT等数据为基础,通过遥感解译和反演得到1999年和2013年研究区内绿地及地表温度空间分布及演变特征,结合温度-植被指数（TVX）空间分析法测度城区发展过程中绿色空间减退所引起的热环境效应,以提出更有针对性的城市气候变化适应性规划。研究结果表明：（1）1999-2013年,研究区绿地面积减少43.8%,绿地斑块增加42.9%,城市绿地呈现面积减少、空间分布碎化的演变特征。其中,森林绿地、公园绿地和街区绿地转化为建设用地的贡献率分别为43.8%、42.9%和13.3%,街区绿地因原始面积小且转化率高,被建设用地侵占最为严重。（2）1999-2013年,研究区地表温度最高值上升5.2 K,地表温差增加1.7 K,地温聚集区间由301.6 ~304.7 K上升至306.5~310.9 K,热环境效应加剧。（3）TVX模型追踪结果显示,快速城市化过程中研究区内城市绿地转化为建设用地引起植被覆盖度下降和地表温度上升,其中街区绿地被侵占造成的地表热环境效应大于公园绿地和森林绿地。     

罗氏沼虾溞状幼体的形态特征

罗氏沼虾的幼体变态发育需经过多次蜕皮,每次蜕皮均伴随形态及附肢特征的改变。采用显微观测摄像法对Z1-Z12各期幼体样本30尾进行观察测量,结果显示:Z2与Z1在复眼上存在较大的差异;Z3的主要特征为尾节与第六腹节分界;Z4尾扇分为内肢和外肢,均具羽状刚毛;Z5无腹足,尾节狭长,且末端平直;Z6的腹部出现腹足萌芽;Z7腹肢芽延长分为内外肢,Z7与Z8的分期特征主要位于第1触角的内鞭、外鞭的分枝上;Z8的第1、第2对步足具不完全的螯;Z9第1、2对步足具完全的螯;Z10后额角上缘的齿刻增多,Z11第1触角内鞭和外鞭分节数量比Z10多;Z12的形态与成虾相似,额角上、下缘均有齿刻。     

北京城市绿化的时空动态分析

城市绿化覆盖是城市生态系统的重要组成部分,合理的绿化率和绿化布局可以改善城市环境,提高城市人居适宜性。研究中将2005、2010、2015年北京市土地利用（LUC）数据中的城市居民用地作为城区范围,应用Landsat 5、GF-1影像数据和MODIS产品,利用支持向量机的监督分类方法,提取了2005、2010、2015年的北京市城市绿化覆盖数据,并获取了同期的植被指数（NDVI）数据;继而以城市绿化覆盖率、绿化覆盖均匀度和植被指数为评价指标,在公里栅格和行政区2个尺度上探讨了北京市城市绿化覆盖的空间分布格局和时间变化动态特征。研究表明：① 3个指标在空间和时间2个维度、区县和栅格2个尺度上都表现一致。这反映北京市过去10年中,在绿化面积增加的同时,绿化的空间布局得到优化改善,绿化的质量得到提高。② 2005-2015年,北京市城市绿化覆盖面积由518.93 km² 增加到1405.54 km²,绿化覆盖率由39.9%增加到49.13%,绿化覆盖均匀度由0.598增加到0.653,植被指数由0.42增加至0.5。③ 北京市城市绿化建设存在明显的时空差异。中心城区绿化建设缓慢,成效不明显;重大绿化建设成果主要集中在城市边缘地区和远郊区县。城市绿化改善过程主要发生在2005-2010年。     

SPERMIOGENESIS IN THE YELLOWFIN PORGY (SPARUS LATUS HOUTTUYN), WITH EMPHASIS ON THE ASSOCIATED MITOCHONDRION

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基于改进引力模型的公园绿地空间可达性及供需平衡分析方法

探究高密度城市公园绿地资源空间配置的有效性和供需平衡问题对城市规划和居民生活具有重要意义。已有研究较少从多尺度、多交通出行模式以及结合地图大数据算法的角度对公园绿地可达性进行探究。本文以广州市越秀区为例,利用百度地图多交通出行数据,提出一种结合多类型公园绿地多级可达时间阈值的改进型引力模型,对越秀区公园绿地的空间可达性及其供需平衡特征进行研究。结果表明：① 越秀区各尺度和各类型公园绿地可达性差异显著,城区尺度可达性大体上呈现由北向南逐层递减趋势,街道尺度可达性则表现出明显的“极化”现象,大型公园绿地可达性远高于社区公园和街头游园且在城市整体可达性中占主体地位;② 从空间集聚情况来看,城区尺度公园绿地可达性值呈现明显的空间集聚分布特征,由北向南表现为高值聚类、不显著、低值聚类,街道尺度相比城区尺度高值聚类减少,低值聚类和无统计意义的区域增多;③ 基于供需平衡视角的空间可达性结果显示,公园绿地供给与居民需求在城区尺度和街道尺度分别呈现出整体平衡和局部失衡的现象,局部失衡主要集中在公园绿地资源相对匮乏的街道。     

Evaluation and Influence Factor of Green Efficiency of China's Agricultural Innovation from the Perspective of Technical Transformation

Agricultural innovation is important for the green transformation of agriculture. Based on the perspective of technology transformation, this paper builds a theoretical analysis framework and evaluation index system for green efficiency of agricultural innovation,and discusses the evolution laws and influencing factors of the green efficiency of China's agricultural innovation from 2005 to 2017 utilizing the DEA model, Malmquist index, and Tobit regression analysis. The results show that: 1) The overall green efficiency of China's agricultural innovation is not high, the green efficiency of agricultural innovation in eastern China is mainly driven by pure technical efficiency, while that in central and western China is mainly driven by the scale efficiency. The green efficiency of agricultural innovation shows significant spatial differences, and the low efficiency and relatively low-efficiency regions moved to central and southeastern China. 2) Technical progress is the main force affecting the change of green total factor productivity of China's agricultural innovation, seeing a trend of decrease followed by an increase. Pure technical efficiency and scale efficiency exhibit an increasing-decreasing trend, and gradually transform into key factors that restrict the improvement of the green total factor productivity of agricultural innovation. 3) Agricultural technologies' diffusion, absorption, and implementation are three influencing factors of the green efficiency of agricultural innovation. The local level of informatization, the number of agricultural technicians in enterprises and institutions, average education level of residents, and the level of agricultural mechanization have positive impacts on the promotion of the green efficiency of agricultural innovation, promoting the diffusion, absorption and implementation of agricultural innovation technology can significantly improve the green efficiency of agricultural innovation.     

Mineral Components and Anti-oxidant Activities of Tropical Seaweeds

Seaweeds are known to hold substances of high nutritional value; they are the richest resources of minerals important to the biochemical reactions in the human body. Seaweeds also hold non-nutrient compounds like dietary fiber and polyphenols. However, there is not enough information on the mineral compounds of tropical seaweeds. Also we are interested in the antioxidant activities of seaweeds, especially those in the tropical area. In this study, Indonesian green, brown and red algae were used as experimental materials with their mineral components analyzed by using an atomic absorption spectrophotometer. The catechins and flavonoids of these seaweeds were extracted with methanol and analyzed by high performance liquid chromatography （HPLC） ; the anti-oxidant activities of these seaweeds were evaluated in a fish oil emulsion system. The mineral components of tropical seaweeds are dominated by calcium, potassium and sodium, as well as small amounts of copper, iron and zinc. A green alga usually contains epigallocatechin, gallocatechin, epigallocatechin gallate and catechin. However, catechin and its isomers are not found in some green and red algae. In the presence of a ferrous ion catalyst, all the methanol extracts from the seaweeds show significantly lower peroxide values of the emulsion than the control, and that of a green alga shows the strongest anti-oxidant extract of this alga, which is significantly different from activity. The highest chelation on ferrous ions is also found in the the other methanol extracts in both 3 and 24 h incubations.     

基于城市园林树木景观三维模拟的绿视率估算方法

城市生态景观功能主要是绿色植物对人的视觉作用。绿视率被认为是一种比较好的衡量绿色空间视觉感受的描述因子。目前绿视率的估算主要基于静态的图像或者街景数据,而绿视率是一种动态的量,不同视点有不同的值,且植物是生长变化的。因此,本文提出一种基于数据和模型综合驱动的园林树木三维模拟景观的绿视率计算方法,其利用虚拟地理环境、虚拟植物等技术,通过道路、建筑物等硬质景观数据和树木模型驱动,建立城市园林树木三维景观;根据视觉成像原理,构建虚拟相机,模拟不同视点园林景观的视觉图像,然后识别表征植被信息的像素,从而计算绿视率。本文研发了园林树木景观三维模拟及绿视率估算原型系统,并以城市道路树木景观为例,模拟分析了机动车道中车辆乘客可获得的绿视率,与街景图像提取的绿视率值相近。该方法和系统可用于包含时间变量的不同生长阶段园林树木景观的绿视率评估,支持交互设置不同的视点参数,估算城市任意位置和任意方向的绿视率,评价过去、现在和未来的园林景观绿化质量,以人的视觉感知角度为城市绿地规划提供参考。     

EFFECTS OF DCMU ON CHLOROPHYLL FLUORESCENCE RATIO F685/F735 IN MARINE RED,BROWN AND GREEN ALGAE

The chorophyll fluorescence ratio F685/F735 in vivo can be a useful indicator for stress detection inhigher plants and seaweed. DCMU [3 - (3, 4 - dichlorophenyl ) - 1, 1 - dimethylurea] treatment influencesthis ratio. Th effects of DCMU on F685/F735 of marine red, brown and green algae under excitationlight of different wavelengths were investigted. In the brown algae, Laminaria japonica and Undariapinnatifida,DCMU did not increase this ratio under blue light excitation but increased the ratio slightlyunder excitation by green light. For the red algea, Halymenia sinensis, DCMU increased the ratiomarkedly under both blue and green ligh excitation. The percentage increase could reach 50% (undergreen light excitation) and was due to unequal enhancement at the two emission maxima by DCMU. Afraction of chlorophyll which contributed to fluorescence in the 735 nm region was less sensitive toDCMU and was likely from photosystem I of red algae. In the green alga, Ulva pertusa, DCMU causeda slight increase in F685     

利用伪归一化差异水体指数提取城镇周边细小河流信息

通过对归一化差异水体指数NDWI中的绿波段修正,提出了不依赖于中红外波段的伪归一化差异水体指数FNDWI（False NDWI）。使用NDWI和FNDWI分别在背景地物为城市、城郊、乡镇、村落和山区的遥感影像上进行河流水体提取,实验表明,FNDWI影像中城镇建筑用地与河流水体的可分离性较NDWI有所提升,提升率为116%~335%不等;相关性分析表明,河流宽度与可分离性提升率具有明显的负相关关系,相关系数为-0.82;分类结果显示,在城市和城郊区域,NDWI提取的水体中混杂有较多城镇建筑用地信息,而FNDWI提取的水体中基本未见混杂。总体上,FNDWI提高了2种地物的可分离性,剔除了NDWI影像混入的城镇建筑用地信息,较好地解决了NDWI城镇建筑用地与河流水体的混淆问题,尤其适用于城镇周边的细小河流。     

Green Tourism in Mountain Regions - Reducing Vulnerability and Promoting People and Place Centric Development in the Himalayas

In recent years, mountain regions are attracting great attention to Indian tourists in general and foreign tourists in particular. The potential mountain resources for promoting green tourism are enormous in the form of natural and cultural heritage such as biosphere reserves, flora and fauna, lakes and rivers and traditional rural resources. In order to utilise tourism industry market, uncontrolled numbers of tourists and related haphazard infrastructural facilities in the vulnerable mountain regions pose serious environmental implications. The ecological pressures are threatening land, water and wild life resources through direct and indirect environmental impacts together with generation of solid and liquid wastes, so green tourism is emerging as an important task in order to develop new relationship between communities, government agencies and private sectors. The strategy focuses on ecological understanding, environmental protection and ecodevelopment. The major attributes of the green tourism include environmental conservation and education and distribution of income to local people based on strong partnership. Various knowledge systems go a long way for achieving the goals of the green tourism, which creates awareness about the value of environmental resources. Mountains have ecological, recreational, educational and scientific values, which need to be utilised in sustainable way. Various tourist activities andfacilities need to be diversified in order to achieve multiple benefits including scientific field excursion, recreation in natural and cultural areas, community festivals and sport tourisms. Green tourism considers tourism development as an integral part of a national and regional development. The paper discusses the social, economic and environmental dimensions of the green tourism with particular reference to village tourism development programme taking empirical evidences from the Himalaya. Such programme also minimises biophysical and human vulnerability and risks in mountain regions. The environmental consciousness campaign and introduction of code through multi- purpose Tourist Resource Centres are gaining currency in above context.     

Spatial gradient analysis of urban green spaces combined with landscape metrics in Jinan City of China

1IN T R O D U C T IO NUrban green spaces can be consideredas the outdoorplacesintheurban covered with significanatmounts ofvegetation,naturalor manmade,as opposed to areasthatare paved or having buildingon them(JIM andC HEN,2003;SHIVANAND and SUZANA,2005)…     

一种基于移动窗口的城市绿度遥感度量方法

针对城市绿度空间分布特征难以描述且合理性难以度量的问题,在基于多光谱遥感数据和激光雷达数据（LiDAR）获取高精度城市建筑物与绿地信息的基础上,本文提出一种基于移动窗口的城市绿度遥感度量方法。其通过遍历研究区像元,建立了移动窗口内的城市绿地面积与固定窗口面积之间的关系,计算了中心像元的绿度指数,并与传统的格网法、缓冲区法进行了比较。研究结果表明：该方法建立的绿度指数,能很好地反映城市居民在区域内接触城市绿地的概率及其空间分布特征,与格网法和缓冲区法相比,有效避免了格网法产生的边缘效应,并解决了缓冲区法空间不连续性的问题,实现了区域范围内任意点的城市绿度度量。该方法为城市绿地度量、城市规划、管理提供了一种新视角。     

Contemporary climatic change over the qinghai-xizang plateau and its response to the green-house effect

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QuickBird 影像城市阴影信息的提取与消除

城市绿地信息在城市研究中的重要作用。但由于各种因素的影响,城市绿地信息提取的精度受到很大的限制,其中,城市中建筑物的阴影是城市绿地信息提取的一个重要限制因素。本研究选取呼和浩特市城区的QuickBird影像,在获取最佳波段组合的基础上,利用多种方法对遥感影像的阴影信息进行提取和消除,以期获得最佳的阴影消除方法,高效地提取城市绿地信息。首先,通过比值运算、波段重组,增强处理影像阴影信息,用最佳指数法分析QuickBird影像阴影提取的最佳波段组合;然后,根据阴影在近红外波段的最小亮度值与最大亮度值的范围建立掩膜,成功提取影像的阴影信息;最后,将色彩空间变换分别与同态滤波和Gamma矫正结合以消除影像阴影,并与其他方法进行对比。研究结果表明,QuickBird影像阴影提取的最佳波段组合为3/4、4、2波段组合,最佳亮度值范围为70-165;色彩空间变换与Gamma矫正相结合的方法可更好地消除阴影,并能较好地保留影像的彩色信息,是消除阴影的最佳方案。     

GPS长距离基线精确解算的有关问题探讨

探讨了GPS长距离基线精确解算方法及其解算精度与观测时间的关系.研究表明,充分利用载波相位各组合观测值特点的L5-L3解法比L3解法好;长距离基线解算时,观测时间达到一定长度后,其精度趋于稳定,再观测更长时间精度提高很少,甚至不提高.