Cavitron extraction of xylem water suggests cryogenic extraction biases vary across species but are independent of tree water stress

Cryogenic vacuum distillation (CVD) is a widely used technique for extracting plant water from stems for isotopic analysis, but concerns about potential isotopic biases have emerged. Here, we leverage the Cavitron centrifugation technique to extract xylem water and compare its isotopic signature to that of CVD-extracted bulk stem water as well as source water. Conducted under field conditions in tropical northern Australia, our study spans seven tree species naturally experiencing a range of water stress levels. Our findings reveal a significant deuterium bias in CVD-extracted bulk stem water when compared to xylem water (median bias −14.9‰), whereas xylem water closely aligned with source water (median offset −1.9‰). We find substantial variations in deuterium bias among the seven tree species (bias ranging from −19.3‰ to −9.1‰), but intriguingly, CVD-induced biases were unrelated to environmental factors such as relative stem water content and predawn leaf water potential. These results imply that inter-specific differences may be driven by anatomical traits rather than tree hydraulic functioning. Additionally, our data highlight the potential to use a site-specific deuterium offset, based on the isotopic signature of local source water, for correcting CVD-induced biases.     

Comparison of root water uptake functions to simulate surface energy fluxes within a deep‐rooted desert shrub ecosystem

Root water uptake (RWU) is a unique process whereby plants obtain water from soil, and it is essential for plant survival. The mechanisms of RWU are well understood, but their parameterization and simulation in current Land Surface Models (LSMs) fall short of the requirements of modern hydrological and climatic modelling research. Though various RWU functions have been proposed for potential use in LSMs, none was proven to be applicable for dryland ecosystems where drought was generally the limiting factor for ecosystem functioning. This study investigates the effect of root distribution on the simulated surface energy fluxes by incorporating the observed vertical root distribution. In addition, three different RWU functions were integrated into the Common Land Model (CLM) in place of the default RWU function. A comparison of the modified model's results with the measured surface energy fluxes measured by eddy covariance techniques in a Central Asian desert shrub ecosystem showed that both RWU function and vertical root distribution were able to significantly impact turbulent fluxes. Parameterizing the root distribution based on in‐situ measurement and replacing the default RWU function with a revised version significantly improved the CLM's performance in simulating the latent and sensible heat fluxes. Sensitivity analysis showed that varying the parameter values of the revised RWU function did not significantly impact the CLM's performance, and therefore, this function is recommended for use in the CLM in Central Asian desert ecosystems and, possibly, other similar dryland ecosystems. Copyright © 2013 John Wiley & Sons, Ltd.     

Using stable isotopes to determine the water sources in alpine ecosystems on the east Qinghai‐Tibet plateau,China

To investigate the water circulation of eastern Qinghai‐Tibet plateau during rainy season, water samples of precipitation, throughfall, fog, soil, litter and xylem were collected for stable isotope analysis. The results showed that precipitation mainly originated as a result of the East Asian Monsoon, and the secondarily evaporated water from subalpine ecosystem was an important part in local atmospheric water cycle. The deuterium excess of rainfall in the alpine meadow was evidently higher than the precipitation in the Dengsheng stations. This suggests that a large part of precipitation in alpine meadow was derived from secondarily evaporated water and the mean contribution was 39·57%, about 3·65 mm produced shortly after rain events. Through the contrast of delta (d)‐excess value in different water samples, it could be concluded that the water in subalpine shrubland and transpiration of subalpine dark coniferous forest were the main source of secondarily evaporated water that transferred to alpine meadow. Hence, the precipitation on the east Qinghai‐Tibet plateau was doubly controlled by monsoon and local water circulation in alpine ecosystems. Copyright © 2010 John Wiley & Sons, Ltd.     

Water isotopic composition traces source and dynamics of water supply in a semi-arid agricultural landscape

Changes in seasonality and form of precipitation alter the structure and function of grassland and steppe ecosystems and pose challenges for land management and crop production in regions like the Northern Great Plains, North America. This research uses isotopic composition of water (δ¹⁸O and δ²H) to explore the sources and fate of soil water in lower-elevation agricultural areas of the Judith River watershed, in the headwaters of the Missouri River, USA. Extensive non-irrigated cereal crop production in this area occurs on well-drained soils and depends on careful water management. Our observations indicate that colder precipitation contributes isotopically distinct water to cultivated terrace soils relative to downgradient groundwaters and streams. Riparian waters also exhibit a higher fraction of contributions from colder precipitation relative to terrace groundwaters and streams. Apparent contributions from colder precipitation in terrace and riparian soil waters suggest that snowmelt is a key component of the water supply to these systems. Riparian waters also show evidence of evaporation suggesting that water spends sufficient time in some ponds and open channels in the riparian corridor to reflect fractionation by evaporation. The evolution of water isotopic composition from soils to shallow aquifers to stream corridors indicates source water partitioning as precipitation moves through this semi-arid agricultural landscape. The apparent mixing processes evident in this evolution reveal source water dynamics that are necessary to understand plant transpiration, solute processing, and contaminant leaching processes.     

Impact of floodwaters on vertical water fluxes in the deep vadose zone of an alluvial aquifer in a semi-arid region

The spatial and temporal variation of moisture distribution, overall water balance and quantity of infiltrated water in the vadose zone of the Sidi Bouzid Plain (Tunisia) during successive flooding events is quantified in this study. The variation in water content in response to environmental factors such as evaporation and water root uptake is also highlighted. One-dimensional flow simulations in the deep vadose zone were conducted at three spreading perimeters located near Wadi El Fekka. The hydraulic boundary conditions of a time-dependent water blade applied to the soil surface were determined from measured flood hydrographs. For the chosen wet year, the successive flooding events contributed to a significant artificial recharge of the natural groundwater. Although the soil hydraulic parameters did not vary strongly in space, flow simulations showed significant differences in the overall water balance of approximately 9–16% for the various spreading perimeters.     

The effects of soil organic matter on soil water retention and plant water use in a meadow of the Sierra Nevada,CA

Tuolumne Meadows is a groundwater dependent ecosystem in the Sierra Nevada of California, USA, that is threatened by hydrologic impacts that may lead to a substantial loss of organic matter in the soil. In order to provide a scientific basis for management of this type of ecosystem, this paper quantifies the effect of soil organic content on soil water retention and water use by plants. First, we show a substantial dependence of soil water retention on soil organic content by correlating Van Genuchten soil water retention parameters with soil organic content, independent of soil texture. Then, we demonstrate the impact of organic content on plants by simulating the degree to which root water uptake is affected by soil water retention with the use of a physically based numerical model of variably saturated groundwater flow. Our results indicate that the increased water retention by soil organic matter contributes as much as 8.8 cm to transpiration, or 35 additional water‐stress free days, during the dry summer when plants experience increased water stress.     

Dependence of shrub canopy water storage on raindrop size in revegetated desert

Abstract

The aim of this study was to evaluate canopy water storage (CWS) of the co-dominant shrubs in the revegetation of sand dunes in northwest China. Our results indicated that CWS differed among the xerophyte taxa studied. The average CWS increased exponentially with decreased raindrop size. The time course of CWS in terms of leaf area indicated that Artemisia ordosica attains its peak value of 0.48 mm within 170 min. The corresponding values for Caragana korshinskii and Hedysarum scoparium were 0.38 mm and 178 min, and 0.32 mm and 161 min, respectively, implying that A. ordosica had a higher CWS than C. korshinskii and H. scoparium. Dry biomass was a desirable predictor for estimation of CWS for C. korshinskii and H. scoparium, and shrub volume for A. ordosica. Our results show that the dependence of CWS on raindrop size varied in accordance with the shrub canopy structure.

Editor Z.W. Kundzewicz     

Varying water utilization of Haloxylon ammodendron plantations in a desert‐oasis ecotone

Haloxylon ammodendron is a desert shrub used extensively in China for restoring degraded dry lands. An understanding of the water source used by H. ammodendron plantations is critical achieving sustainable vegetation restoration. We measured mortality, shoot size, and rooting depth in 5‐, 10‐, 20‐, and 40‐year‐old H. ammodendron plantations. We examined stable isotopic ratios of oxygen (δ¹⁸O) in precipitation, groundwater, and soil water in different soil layers and seasons, and in plant stem water to determine water sources at different shrub ages. We found that water acquisition patterns in H. ammodendron plantations differed with plantation age and season. Thus, the main water source for 5‐year‐old shrubs was shallow soil water. Water sources of 10‐year‐old shrubs shifted depending on the soil water conditions during the season. Although their tap roots could absorb deep soil water, the plantation main water sources were from soil water, and about 50% of water originated from shallow and mid soil. This pattern might occur because main water sources in these plantations were changeable over time. The 20‐ and 40‐year‐old shrubs acquired water mainly from permanent groundwater. We conclude that the main water source of a young H. ammodendron plantation was soil water recharged by precipitation. However, when roots reached sufficient depth, water originated mainly from the deep soil water, especially in the dry season. The deeply rooted 20‐ and 40‐year‐old shrubs have the ability to exploit a deep and reliable water source. To achieve sustainability in these plantations, we recommend a reduction in the initial density of H. ammodendron in the desert‐oasis ecotone to decelerate the consumption of shallow soil water during plantation establishment.     

Water use characteristics of coexisting sand-binding vegetation in two typical soil habitats in the desert steppe of China

Understanding the water use characteristics and water relationship of coexisting vegetation in a mixed-species plantation of trees and shrubs is crucial for the sustainable restoration of degraded arid areas. This study investigated the water use characteristic of coexisting sand-binding vegetation combinations in the sierozem habitat (Populus przewalskii Maxim namely Populus-S and Caragana liouana) and aeolian sandy soil habitats (Populus przewalskii Maxim namely Populus-A and Salix psammophila) of the desert steppe. By analysing the δ²H and δ¹⁸O isotopes in xylem, soil water, groundwater and precipitation, a Bayesian MixSIAR model was employed to quantitatively assess the water utilization characteristics of plants. Throughout the growing season, in the sierozem habitat, C. liouana exhibits the highest efficiency in utilizing soil moisture above 60 cm (53.45%) and displays adaptable water use strategies. In contrast, Populus-A predominantly relies on deep soil moisture below 60 cm plus groundwater (63.89%). In the aeolian sandy soil habitat, both Populus-A and S. psammophila similarly favour deep soil moisture below the 60 cm soil plus groundwater (66.77% and 67.60%, respectively). During the transition period from the dry to the wet seasons, although both Populus-A and S. psammophila in the aeolian sandy soil habitat shifted their water sources from deeper to shallower ones, there was considerable overlap in the water sources used by Populus-A and S. psammophila. This overlap led to competition for water resources and exacerbated the depletion of deep soil moisture in both seasons. Conversely, in the sierozem habitat, the partitioning of water sources between Populus-S and C. liouana facilitated the allocation and utilization of water resources between the two species. The findings highlight the need for species-specific consideration in water resource allocation within mixed-species plantations of trees and shrubs, which is crucial for sustainable vegetation restoration in sand-binding ecosystems.     

Effects of surface characteristics on infiltration patterns in an arid shrub desert

Precipitation is often the sole source of water replenishment in arid and semi‐arid areas and, thus, plays a pertinent role in sustaining desert ecosystems. Revegetation over 40 years using mainly Artemisia ordosica and Caragana korshinskii at Shapotou Desert Experimental Research Station near Lanzhou, China, has established a dwarf‐shrub and microbiotic soil crust cover on the stabilized sand dunes. The redistribution of infiltrated moisture through percolation, root extraction, and evapotranspiration pathways was investigated. Three sets of time‐domain reflectometry (TDR) probes were inserted horizontally at 5, 10, 15, 20, 30 and 40 cm depths below the ground surface in a soil pit. The three sets of TDR probes were installed in dwarf‐shrub sites of A. ordosica and C. korshinskii community with and without a microbiotic soil crust cover, and an additional set was placed in a bare sand dune area that had neither vegetation nor a microbiotic soil crust present. Volumetric soil moisture content was recorded at hourly intervals and used in the assessment of infiltration for the different surface covers. Infiltration varied greatly, from 7·5 cm to more than 45 cm, depending upon rainfall quantity and soil surface conditions. In the shrub community area without microbiotic soil crust cover, infiltration increased due to preferential flow associated with root tunnels. The microbiotic soil crust cover had a significant negative influence on the infiltration for small rainfall events (～10 mm), restricting the infiltration depth to less than 20 cm and increasing soil moisture content just beneath the soil profile of 10 cm, whereas it was not as strong or clear for larger rainfall events (～60 mm). For small rainfall events, the wetting front depth for the three kinds of surface cover was as follows: shrub community without microbiotic soil crust > bare area > shrub community with microbiotic soil crust. In contrast, for large rainfall events, infiltration was similar in shrub communities with and without microbiotic soil crust cover, but significantly higher than measured in the bare area. Soil water extraction by roots associated with evapotranspiration restricted the wetting front penetration after 1 to 3 h of rainfall. Copyright © 2006 John Wiley & Sons, Ltd.     

Water uptake by saltcedar (Tamarix ramosissima) in a desert riparian forest: responses to intra‐annual water table fluctuation

There is considerable interest in naturalizing flow regime on managed rivers to slow the spread of saltcedar (Tamarix ramosissima) invasion in southwestern USA or to preserve riparian forests dominated by saltcedar and other species in northwestern China. However, little is known about the responses of established saltcedar in water sources to frequent intra‐annual fluctuation of water table resulting from this new, more dynamic flow regime. This study investigates how saltcedar at a riparian site in the middle reaches of the Heihe River, northwest China, responds in water sources use to intra‐annual water table fluctuations. Stable oxygen isotope was employed to determine accurate depth at which saltcedar obtains its water supply, and soil moisture monitoring was used to determine sources of plant‐available soil water. We found that the primary zone of water uptake by saltcedar were stable at 25–60 cm depth, but the water sources used by saltcedar switched between groundwater and soil moisture with the water table fluctuations. Saltcedar derived its water from groundwater when water table was at depth less than 60 cm but switched to soil moisture at 25–60 cm depth when water table declined. It is supposed that the well‐developed clay layer at 60–80 cm depth constrained lateral roots of saltcedar to the soil layers above 60 cm, while the fine‐textured soils at this site, which were periodically resaturated by rising groundwater before the stored soil moisture had become depleted, provided an important water reservoir for saltcedar when groundwater dropped below the primary zone of fine roots. The root distribution of saltcedar may also be related to local groundwater history. The quick decline in water table in the early 1980s when the riparian saltcedar had established may strand its roots in the shallow unsaturated zone. We suggested that raising the water table periodically instead of maintaining it invariably above the rooting depth could sustain desired facultative phreatophytes while maximizing water deliveries. Copyright © 2015 John Wiley & Sons, Ltd.     

Remote sensing and ground‐based measurements of evapotranspiration in an extreme cold Patagonian desert

Accurate estimates of seasonal evapotranspiration (ET) at different temporal and spatial scales are essential for understanding the biological and environmental determinants of ecosystem water balance in arid regions and the patterns of water utilization by the vegetation. For this purpose, remote sensing ET estimates of a Patagonian desert in Southern Argentina were verified with field measurements of soil evaporation and plant transpiration using an open top chamber. Root distribution and seasonal variation in soil volumetric water content were also analysed. There was a high correlation between remote sensing and field measurements of ecosystem water fluxes. A substantial amount of the annual ET occurred in spring and early summer (73.4 mm) using winter rain stored in the soil profile and resulting in water content depletion of the upper soil layers. A smaller amount of annual ET was derived from few rainfall events occurring during the mid or late summer (41.4 mm). According to remote sensing, the 92.9% of the mean annual precipitation returns to the atmosphere by transpiration or evaporation from the bare soil and by canopy interception. Only 7.1% infiltrates to soil layers deeper than 200 cm contributing to the water table recharge. Fourier time series analysis, cross‐correlation methods and multiple linear regression models were used to analyse 11 years of remote sensing data to assess determinants of water fluxes. A linear model predicts well the variables that drive complex ecosystem processes such as ET. Leaf area index and air temperature were not linearly correlated to ET because of the multiple interaction among variables resulting in time lags with ET variations and thus these two variables were not included in the linear model. Soil water content, the fraction of photosynthetic active radiation and precipitation explained 86% of the ET monthly variations. The high volumetric water content and the small seasonal variations at 200‐cm depth were probably the result of little water uptake from deeper soil horizons by roots with low hydraulic conductivity. Copyright © 2016 John Wiley & Sons, Ltd.     

Identifying the origin of groundwater for water resources sustainable management in an arid oasis,China

ABSTRACT

Many oases are experiencing severe groundwater depletion due to increased population, expanding agriculture and economic development. For sustainable development, quantifying groundwater recharge resources are fundamentally important. In this study, stable isotope techniques were employed to identify recharge sources of groundwater and quantitatively evaluate their contribution ratios in the Dunhuang Oasis, northwest China. Our findings indicate that heavy isotopes in shallow groundwater are more negative than those in deep groundwater, which is attributed to shallow groundwater that was modern and deep groundwater that was old. Irrigated return water and lateral groundwater flow from the Qilian Mountains are considered as the two main sources of shallow groundwater, accounting for 35% and 65% of the total recharge, respectively. Thus, as the main groundwater source of the Dunhuang Oasis, the Qilian Mountain Front should be protected against over-exploitation. Our results provide not only fundamental knowledge for groundwater management of aquifers of the Oasis, but also valuable water management information for other similar arid oases worldwide.     

Characterizing spatial and temporal variation in 18O and 2H content of New Zealand river water for better understanding of hydrologic processes

Time series of hydrogen and oxygen stable isotope ratios (δ²H and δ¹⁸O) in rivers can be used to quantify groundwater contributions to streamflow, and timescales of catchment storage. However, these isotope hydrology techniques rely on distinct spatial or temporal patterns of δ²H and δ¹⁸O within the hydrologic cycle. In New Zealand, lack of understanding of spatial and temporal patterns of δ²H and δ¹⁸O of river water hinders development of regional and national-scale hydrological models. We measured δ²H and δ¹⁸O monthly, together with river flow rates at 58 locations across New Zealand over a two-year period. Results show: (a) general patterns of decreasing δ²H and δ¹⁸O with increasing latitude were altered by New Zealand's major mountain ranges; δ²H and δ¹⁸O were distinctly lower in rivers fed from higher elevation catchments, and in eastern rain-shadow areas of both islands; (b) river water δ²H and δ¹⁸O values were partly controlled by local catchment characteristics (catchment slope, PET, catchment elevation, and upstream lake area) that influence evaporation processes; (c) regional differences in evaporation caused the slope of the river water line (i.e., the relationship between δ²H and δ¹⁸O in river water) for the (warmer) North Island to be lower than that of the (cooler, mountain-dominated) South Island; (d) δ²H seasonal offsets (i.e., the difference between seasonal peak and mean values) for individual sites ranged from 0.50‰ to 5.07‰. Peak values of δ¹⁸O and δ²H were in late summer, but values peaked 1 month later at the South Island sites, likely due to greater snow-melt contributions to streamflow. Strong spatial differences in river water δ²H and δ¹⁸O caused by orographic rainfall effects and evaporation may inform studies of water mixing across landscapes. Generally distinct seasonal isotope cycles, despite the large catchment sizes of rivers studied, are encouraging for transit time analysis applications.     

Deep soil water dynamics in an undisturbed primary forest in central Amazonia: Differences between normal years and the 2005 drought

Understanding how Amazonian rainforests deal with extended droughts is critical in the face of changing climate. This research analyze the physical properties and the soil water dynamics of a deep soil profile in an area of primary forest in central Amazonia to elucidate these processes under drought and nondrought conditions. Physical soil properties derived from soil cores exhibited a distinctive layer between 480 and 880 cm deep, characterized by higher microporosity and low plant water availability. In situ soil moisture measurements collected during the period from January 2003 through February 2006 and for depths ranging from 10 to 1,430 cm suggest that, in the study site, the top 480 cm of the soil profile satisfied most of the transpirational demands in normal climatological years. However, during exceptionally dry periods, such as the 2005 drought, root uptake occurs below 480 cm. As concluded by previous studies, most of the uptake is concentrated in the first meter of the soil profile: More than 40% of the total demand for transpiration is supplied by the top meter of soil. Because deep root uptake occurred at greater depths than normal during the 2005 drought, our results suggest that this is a fundamental mechanism to cope with prolonged droughts.     

Variation and attribution of water use efficiency in sunflower and maize fields in an irrigated semi-arid area

Water use efficiency (WUE) links carbon and water exchanges between farmlands and the atmosphere. Understanding the variation and attribution of WUE is essential to reveal the physiological and ecological adaptation mechanisms of crops to the changing environment, and to better allocate, regulate and conserve water resources. However, few studies on the variation and attribution of WUE have been conducted in irrigated arid or semi-arid farmlands. Therefore, in this study, water and carbon fluxes were measured using eddy covariance systems in two farmlands (one sunflower field and one maize field) in a semi-arid irrigation district in China. It was found that the average WUE of sunflower during its full growth period was 1.72 g C kg⁻¹ H₂O, much lower than that of maize (4.07 g C kg⁻¹ H₂O). At each growth stage, the WUE of both crops were negatively correlated with vapour pressure deficit (VPD), net radiation (R_n) and soil water content (SWC). The negative correlations could be attributed to the arid meteorological condition and the relatively abundant soil moisture due to irrigation and shallow groundwater levels. VPD was the main factor affecting WUE, followed by R_n and SWC. It was also found that the response of WUE to crop leaf area index (LAI) and to canopy conductance (g_c) depended on the VPD ranges: when VPD increased, the response of WUE to LAI and to g_c decreased. Our findings could improve the understanding of the coupling effect of water and carbon fluxes over farmland ecosystems in arid and semi-arid irrigation areas and help improve agricultural production and save water resources in such areas.     

How do non-halophyte locust trees thrive in temperate coastal regions: A study of salinity and multiple environmental factors on water uptake patterns

Understanding plant water use patterns is crucial for comprehending the dynamics of the soil–plant-atmosphere continuum and evaluating the adaptability of plants across diverse ecosystems. However, there remains a gap in our comprehension of non-halophyte plants' water uptake patterns and driving factors in temperate coastal regions. For this reason, we used locust trees (a widely planted non-halophyte tree species in northern China) as a study subject. We collected water isotope data (δ²H and δ¹⁸O) for locust trees xylem and soil over two consecutive growing seasons. The MixSIAR model was used along with five distinct sets of input data (single isotopes, uncorrected dual isotopes, and corrected dual isotopes incorporating δ²H data obtained by soil water line or cryogenic vacuum distillation methods) to infer water utilization patterns. The results indicated that locust trees primarily absorb shallow soil water (0–20 cm, 29.4% ± 16.9%) and deep soil water (120–180 cm, 24.7% ± 5.8%). Pearson's correlation analysis revealed the key driving factors behind water uptake patterns were vegetation transpiration and soil salinity. Remarkably, the build up of salts in the lower soil layer (60–120 cm) hinders the absorption of water by plants. To prevent high salt concentrations from affecting water uptake in non-halophyte plants, we recommend implementing sufficient irrigation from March to April each year to meet the water needs of plant growth and regulate the accumulation of salts in various soil layers. This study reveals the dynamic water utilization strategy of non-halophyte plants in temperate coastal regions, offering valuable information for water resources management.     

Seasonal changes in the water use strategies of three co‐occurring desert shrubs

Water is a major limiting factor in desert ecosystems. In order to learn how plants cope with changes in water resources over time and space, it is important to understand plant–water relations in desert region. Using the oxygen isotopic tracing method, our study clarified the seasonal changes in the water use strategies of three co‐occurring desert shrubs. During the 2012 growing season, δ¹⁸O values were measured for xylem sap, the soil water in different soil layers between 0 and 300 cm depth and groundwater. Based on the similarities in δ¹⁸O values for the soil water in each layer, three potential water sources were identified: shallow soil water, middle soil water and deep soil water. Then we calculated the percentage utilization of potential water sources by each species in each season using the linear mixing model. The results showed that the δ¹⁸O values of the three species showed a clear seasonal pattern. Reaumuria songarica used shallow soil water when shallow layer was relatively wet in spring, but mostly took up middle soil water in summer and autumn. Nitraria tangutorum mainly utilized shallow and middle soil water in spring, but mostly absorbed deep soil water in summer and autumn. Tamarix ramosissima utilized the three water sources evenly in spring and primarily relied on deep soil water in summer and autumn. R. songarica and N. tangutorum responded quickly to large rainfall pulses during droughts. Differential root systems of the three species resulted in different seasonal water use strategies when the three competed for water. Copyright © 2013 John Wiley & Sons, Ltd.