STUDY ON ~(210) Po IN MARINE ORGANISMS IN NORTH CHINA WATERS

INTRODUCTIONWiththedevelopmentofcoastalnuclearpowerandmarinenuclearestablishments,peoplepaymoreandmoreattentiontothedistribution ,behaviorandpathwaysofsomeman maderadionuclidesinmarineenvironment.Thedischargedartificialradionuclidesoverlieabackgroundofnaturallyoccurringradionu clideswhicheitheralreadyexistedinmarineenvironmentorwasenhancedbysomeotherhumanactivi ties.Goodunderstandingofsomeman incurredradionuclides’impactsonpeopleandmarineorganismsrequiresknowledgeofsomecriticalnaturalradi…     

Seasonal export fluxes of size-fractionated particulate derived from polonium-210: A case study in Xiamen Bay

Size-fractionated ²¹⁰Po and ²¹⁰Pb, in the size fractions >0.4 μm, >2 μm and >10 μm, were examined to determine the seasonal variability of particulate fluxes in Xiamen Bay. Good correlations between ²¹⁰Po and particulate organic carbon (POC) or non-Particulate Organic Matter (nPOM) suggested that ²¹⁰Po can be used to trace the export fluxes of POC and nPOM. Both steady-state (SS) model and nSS model were used to evaluate fluxes of size-fractionated ²¹⁰Po, results showed that nSS model was better than the SS model in coastal areas. Based on the nSS model, size-fractionated POC fluxes decreased with increasing particle size. For the particle size studied, maximum POC fluxes occurred in autumn, followed by spring, winter, and summer. Fluxes of nPOM were an order of magnitude higher than the corresponding size-fractionated POC fluxes. Differences between size-fractionated nPOM fluxes indicated that hydrodynamic conditions were the main factor regulating transportation of particulate out of the inner Bay. In winter most particulates, including >10 μm particles, were transported under the strongest hydrodynamic conditions. In contrast, only a fraction of the <2 μm particulates were transported from the inner Bay in spring. This study suggested that ²¹⁰Po is a powerful tracer of seasonal particulate export in coastal seas.     

Modern sedimentation rates and sedimentation feature in the Huanghe River estuary based on210Pb technique

Based on²¹⁰Pb technique, sedimentation rates of 11 cores collected from the Huanghe River estuary were determined. CaCO₃ distribution of 4 cores chosen from among these was measured. Profiles of²¹⁰Pb in the cores showed that the distribution of²¹⁰Pb activity decayed with depth, appeared in stages and in more than one segment. The sedimentation rates,²¹⁰Pb and CaCO₃ data have similar distribution trend in the 4 cores. The profiles of²¹⁰Pb and CaCO₃ were used to study sedimentation intensity, transportation trend of material and sedimentation features in the corring area. Contribution No. 2230 from the Institute of Oceanology, Academia Sinica. This research was supported by the National Natural Science Foundation of China.     

Growth inhibition and oxidative stress in two species of marine diatoms exposed to 1-phenylethanol

1-phenylethanol(1-PEA)is a flavor extensively used in the production of co smetics,beverages,and food.The release of 1-PEA into coastal environments has aroused great concern.However,its potential effects on marine organisms are still unknown.In order to provide a better understanding of the ecological risks of 1-PEA in marine environments,this study determined the toxic effects of 1-PEA on two marine diatoms(Phaeodactylum tricornutum and Skeletonema costatum).The diatoms were grown in culture medium containing different concentrations of 1-PEA for 96 h.The contents of chlorophyll a,chlorophyll c,glutathione(GSH),malondialdehyde(MDA),and the activities of superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GPx),were measured at the end of the exposure period.1-PEA was shown to significantly inhibit the growth of diatoms,with 96-h EC_(50) values of 257.14 mg/L and 126.46 mg/L in P.tricornutum and S.costatum,respectively.In P.tricornutum,the levels of SOD,CAT,GPx,GSH,and MDA were stimulated only when 1-PEA concentrations were close to or greater than the 96-h EC_(50)value.However,in S.costatum,the activities of SOD and CAT,and the syntheses of two chlorophylls were inhibited even at an exposure concentration below the 96-h EC_(50) value.Taken together,these findings indicate a potential ecological risk by discharging 1-PEA into coastal areas and its species-specific toxic effects on marine organisms.     

Seasonal distribution of bioaerosols in the coastal region of Qingdao

Bioaerosols were collected by using a six-stage bioaerosols sampler from September 2007 to August 2008 in the coastal region of Qingdao, China. The terrestrial and marine microbes(including bacteria and fungi) were analyzed in order to understand the distribution features of bioaerosols. The results show that the average monthly concentrations of terrestrial bacteria, marine bacteria, terrestrial fungi and marine fungi are in the ranges of 80–615 CFU m-3, 91–468 CFU m-3, 76–647 CFU m-3 and 231–1959 CFU m-3, respectively. The concentrations of terrestrial bacteria, marine bacteria, terrestrial fungi, marine fungi and total microbes are the highest in each microbial category during fall, high in spring, and the lowest in the summer and winter. The bacterial particles are coarse in spring, autumn and winter. The sizes of fungal particle present the log-normal distribution in all the seasons.     

Bioaccumulation of Pb<Superscript>2+</Superscript> and its effects on growth,morphology and pigment contents of <Emphasis Type="Italic">Spirulina</Emphasis> (<Emphasis Type="Italic">Arthrospira</Emphasis>) <Emphasis Type="Italic">platensis</Emphasis>

A laboratory experiment was conducted to assess the bioaccumulation of Pb²⁺ and its effects on growth, morphology and pigment contents of Spirulina (Arthrospira) platensis. The specimen cultured in Zarrouk liquid medium was treated with various initial metal concentrations (0, 5, 10, 30, 50 and 100 μg mL⁻¹). The growth of S. platensis was adversely affected by Pb²⁺ at high concentrations (30, 50 and 100 μg mL⁻¹). However, at low concentrations (5 μg mL⁻¹), Pb²⁺ could stimulate its growth slightly. The pigment contents (chlorophyll α and β carotene) were decreased in a dose-dependent manner. The highest reductions (67% and 53% respectively in chlorophyll α and β carotene) were observed in 100 μg mL⁻¹ treatment group. The LC₅₀ (96 h) of Pb²⁺ was measured as 75.34 μg mL⁻¹. Apart from a few cases of filament breakages at elevated concentrations (50 and 100 μg mL⁻¹), morphological abnormalities are not specific. Metal bioaccumulation increased with Pb²⁺ concentrations, but decreased with exposure time. The maximum accumulated amount was 188 mg g⁻¹ dry weight. The bioconcentration factor (BCF) reached to a peak at day 2, followed by a gradual reduction for all the exposure concentrations. S. platensis is able to tolerate considerably high Pb²⁺ concentrations. Consequently it can be used as a potential species to remove heavy metal from contaminated waters.     

Reconstructing environmental changes of a coastal lagoon with coral reefs in southeastern Hainan Island

Coastal lagoons with small catchment basins are highly sensitive to natural processes and anthropogenic activities. To figure out the environmental changes of a coastal lagoon and its contribution to carbon burial, two sediment cores were collected in Xincun Lagoon, southeastern Hainan Island and (210) ~Pb activities, grain size parameters, total organic carbon(TOC), total nitrogen(TN), total inorganic carbon(TIC) and stable carbon isotopes(δ~(13)C) were measured. The results show that in 1770–1815, the decreasing water exchange capacity with outer open water, probably caused by the shifting and narrowing of the tidal inlet, not only diminished the currents and fined the sediments in the lagoon, but also reduced the organic matter of marine sources. From 1815 to 1950, the sedimentary environment of Xincun Lagoon was frequently influenced by storm events. These extreme events resulted in the high fluctuation of sediment grain size and sorting, as well as the great variation in contributions of terrestrial(higher plants, soils) and marine sources(phytoplankton, algae, seagrass). The extremely high content of TIC, compared to TOC before 1950 could be attributed to the large-scale coverage of coral reefs. However, with the boost of seawater aquaculture activities after 1970, the health growth of coral species was severely threatened, and corresponding production and inorganic carbon burial flux reduced. The apparent enhanced inorganic carbon burial rate after 1990 might result from the concomitant carbonate debris produced by seawater aquaculture. This result is important for local government long-term coastal management and environmental planning.     

Acute Toxicity of Formalin to the Red-tide Dinoflageilate Prorocentrum minimum Schiller （Protozoa, Mastigophora）

The acute toxic levels of formalin to a marine red-tide dinoflagellate Prorocentrum minimum Schiller (Mastigophora, Dinoflagellida) were determined by linear regression analysis. The data obtained revealed that there is a close regression relationship between logarithmic concentrations of formalin and mortality probit scales of the organism when exposure times are 1, 2, 6, 12, 24 h. The median lethal concentrations (LC50 values) obtained from correlation analysis for these exposure times (with 95% confidence intervals), were 11.83, 6.76, 4.37, 4.27 and 3.98mgL^-1 respectively. A toxicity curve was obtained to connect the exposure time and LC50 value from the correlation between them. The results indicated that P. minimum could be killed or fixed by formalin in the concentration range of 4-12mgL^-1 within 1-24h.     

Effects of Na+/K+ ratio of groundwaters on the gill ion-transport enzyme activity, plasma osmolality and growth of Cynoglossus semilaevis juveniles

The effects of environmental Na⁺/K⁺ ratio on the gill ion-transport enzyme activity, plasma osmolality and growth of Cynoglossus semilaevis juveniles were investigated. The results showed that, plasma osmolality was similar among flounder adapted to different Na⁺/K⁺ ratios of saline groundwaters (P>0.05), while the growth, gill Na⁺, K⁺-ATPase and HCO₃ ⁻-ATPase activities were affected by Na⁺/K⁺ ratio significantly (P<0.05). The gill Na⁺, K⁺-ATPase activity reached its maximum on day 3, then decreased gradually from day 3 to day 9 and remained constant from day 9 to day 15. The peak values of gill Na⁺, K⁺-ATPase activity were detected on day 3 for all Na⁺/K⁺ ratios of saline groundwaters, then the enzyme activities descended, and on day 9 the enzyme activities achieved steady state, and the gill HCO₃ ⁻-ATPase activity increased rapidly and achieved steady state after one day. During steady state, the gill Na⁺, K⁺-ATPase and HCO₃ ⁻-ATPase activity of Na⁺/K⁺ ratios 20 and 40 treatments were significantly higher than those in the control group (Na⁺/K⁺ ratio 27.5), while there were no significant differences between the Na⁺/K⁺ ratio 30 treatment and the control group; the gill Na⁺, K⁺-ATPase activity of Na⁺/K⁺ ratio 20 and 40 treatments were significantly higher than that for ratio 30 treatment, but there were no significant differences of gill HCO₃ ⁻-ATPase activity among these treatments. At the end of the 15-day experiment, the weight gain (%) and specific growth rate (SGR) of flounders maintained in seawater were significantly higher than those in groundwaters; significant differences also occurred among the treatments; Na⁺/K⁺ ratio 30 treatment had the highest values (33.7% and 1.94 respectively), which were significantly higher than those under Na⁺/K⁺ ratios 20 and 40 treatments. Therefore, for the saline groundwater used in this experiment, it is suggested that the Na⁺/K⁺ ratio be adjusted to approximately 30, i.e., as close to that of natural seawater as possible in the culture of flounder.     

Distribution and accumulation characteristics of heavy metals in sediments in southern sea area of Huludao City, China

The southern sea area of the Huludao City, Liaoning Province might be polluted by heavy metals because it is close to the Jinzhou Bay, one of the heaviest sea area polluted by heavy metals in China. The undisturbed modern sediment core can be used to analyze the accumulation and source of the pollutants using 137 Cs and 210 Pb ex . Thirty-five samples of surface sediment and two core sediments were collected from the southern sea area of Huludao City. The concentrations of copper (Cu), lead (Pb), chrome (Cr), zinc (Zn), arsenic (As) and mercury (Hg) in the surface sediments as well as Cu, Pb, Zn, Cr, 137 Cs and 210 Pb ex in the core sediments were determined to research the spatial distribution and accumulation characteristics, and to analyze the sources and the potential risks of heavy metals. The results show that the pollution levels of Zn and Hg are serious, and 26 stations are at moderate or heavy ecological risks. The concentrations of the heavy metals increase from east to west, as well as from open sea to offshore marine area. The concentrations of heavy metals are not high in the sediments adjacent to the Jinzhou Bay, and the influence caused by the seawater exchange with the Jinzhou Bay is little. The concentrations of the heavy metals in the core sediments show low-high-low characteristic, and it coincides with the pollution history of Huludao City. The atmospheric deposition of heavy metals from the Huludao Zinc Plant is likely to be the main source of pollution without direct discharge of wastewater. The high concentrations of heavy metals appear on the upper sediment of 20 cm. The shallow sediment with high heavy metal contents might be exposed to surface when it was disturbed by the ocean engineering and big storm surge, then cause risk to the safety of aquaculture and human healthy.     

Short- and long-term sediment transport in western Bohai Bay and coastal areas

Sediment cores (∼40–100 cm) were collected at 12 locations in the western Bohai Bay, the Haihe River estuary, the Yongding River estuary and the Tianjin Harbor, China, during 24–26 July 2007, and analyzed for ⁷Be and ²¹⁰Pb activities. Due to localized hydrodynamic patterns and frequent disturbance from dredging activities, steady-state sedimentation features were not observed in this study. As demonstrated in the ⁷Be and ²¹⁰Pb profiles, the temporal and spatial variations of these radionuclides support a non-steady state depositional environment in the study area. By comparing ⁷Be and ²¹⁰Pb inventories in the sediments with those of the atmospheric source, we found that: 1) sediments dredged from the Tianjin Harbor or eroded from nearby estuarine and coastal areas are retained in the western Bohai Bay for relatively short intervals (several months), as reflected in the relatively high ⁷Be inventories in the western Bohai Bay; 2) over the long-term (years to decades), ²¹⁰Pb inventories in the sediments imply that there is a net on-shore transport of sediments, and the sediments are mass-balanced in the entire study area. Overall, our results suggest that the sediments are retained in the estuaries and the western Bohai Bay despite local variability in sediment dynamics and disturbance due to human activities.     

The influence of Fe^2＋ on growth and development of cells enzymatically isolated from Porphyra yezoensis blades

Fe^2＋ acted as an accessorial factor for many cellular enzymatic reactions is very important for seaweed growth and development, but the Fe^2＋ requirement in nori had not been seen. Porphyra yezoensis cells were separated enzymatically and cultured in a series of sterilized seawater media containing various concentrations of Fe^2＋. The growth development and cell were investigated in this work. Through this experiment, two biologically-meant concentration scales were found, one is low concentrations, 12.1-102.1μg/L, 10-100 times than that in seawater, favoring the development of isolated cells of Porphyra and the other was high concentrations, more than 10mg/L inhibiting the cell growth, leading to the deformity and shrinkage of the cells. At the concentration of 50 mg/L, the cells stopped growing and died eventually.     

Origins of terrestrial organic matter in surface sediments of the East China Sea shelf

Terrestrial organic matter(TOM) is an important component of marine sedimentary OM, and revealing the origins and transport mechanisms of TOM to the East China Sea(ECS) is important for understanding regional carbon cycle. A novel approach combining molecular proxies and compound-specific carbon isotopes is used to quantitatively constrain the origins and transport mechanisms of TOM in surface sediments from the ECS shelf. The content of terrestrial biomarkers of(C_(27)+C_(29)+C_(31)) n-alkanes(52 to 580 ng g~(-1)) revealed a seaward decreasing trend, the δ~(13)CTOC values(-20.6‰ to-22.7‰) were more negative near the coast, and the TMBR(terrestrial and marine biomarker ratio) values(0.06 to 0.40) also revealed a seaward decreasing trend. These proxies all indicated more TOM(up to 48%) deposition in the coastal areas. The Alkane Index, the ratio of C_(29)/(C_(29)+C_(31)) n-alkanes indicated a higher proportion of grass vegetation in the coastal area; While the δ13C values of C_(29) n-alkane(-_(29).3‰ to-33.8‰) indicated that terrestrial plant in the sediments of the ECS shelf were mainly derived from C_3 plants. Cluster analysis afforded detailed estimates of different-sourced TOM contributions and transport mechanisms. TOM in the Zhejiang-Fujian coastal area was mostly delivered by the Changjiang River, and characterized by higher %TOM(up to 48%), higher %C_3 plant OM(68%-85%) and higher grass plant OM(56%-61%); TOM in the mid-shelf area was mostly transported by aerosols, and characterized by low %TOM(less than 17%), slightly lower C_3 plant OM(56%-72%) and lower grass plant OM(49%-55%).     

Effects of salinity fluctuation frequency on the osmolarity, Na+-K+-ATPase activity and HSP70 expression in juvenile chinese shrimp, Fenneropenaeus chinensis

Experiments were conducted to examine the effects of salinity fluctuation frequency on the osmolarity, Na⁺-K⁺-ATPase activity and HSP70 of Chinese shrimp Fenneropenaeus chinensis with initial wet body weight of 1.460 g ± 0.091 g. The salinity in the control group (D0) was 28 throughout the experiment, whereas treatments D2, D4, D6 and D8 were subjected to different salinity fluctuation frequencies of 2, 4, 6 and 8 d, respectively. The salinity in treatments D2, D4, D6 and D8 was kept at 28 for 2, 4, 6 and 8 d, respectively, decreased abruptly to salinity 24, lasted for another 2 d, and then was raised to its initial value 28. This was a complete salinity fluctuation cycle that afterwards repeated itself. After 32 days, the osmolarity in treatments D2, D4, D6 and D8 was significantly lower than that in treatment D0 (P<0.05). There were significant differences in both muscle and eyestalks HSP70 expression among groups. The HSP70 expressions in muscle and eyestalks in group D4 were 61.4% and 57.0% higher, respectively, than that in the control group D0 (P<0.05). There were, however, no significant differences in gill or hepatopancreas Na⁺-K⁺-ATPase activity between the treatments and the control.     

Acute toxicities of potassium permanganate, formalin, and Lugol’s iodine solution to a marine ciliate, Pleuronema coronatum (ciliophora, scuticociliatida)

Acute toxicities of potassium permanganate, formalin, and Lugol’s iodine solution to a commonly occurred marine ciliate Pleuronema coronatum (Ciliophora, Scuticociliatida) were measured. Linear regression analysis of the results highlighted the close relationships between doses of the medicines and mortalities of the organisms, thus providing a capability to predict toxicity effects from the dose. Toxic effects of the medicines on the ciliates were described in the present paper, and the median lethal concentrations (LC₅₀ values) were given. Results of measurements indicated that 2 h-LC₅₀ and 12 h-LC₅₀ values of formalin on P. coronatum were 59.00×10⁻⁶ and 43.57×10⁻⁶, while those of Lugol’s solutions were 90.13 and 67.84×10⁻⁶ respectively. The tolerance of P. coronatum to formalin is apparently lower than that to Lugol’s iodine solution and potassium permanganate is a suitable medicine to kill ciliates in short time.     

MODERN CLIMATIC SIGNALS DEDUCED FROM STABLE ISOTOPE IN SHELLS IN XINGCUO LAKE SEDIMENTS,EAST TIBETAN PLATEAU,CHINA

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Distribution of heavy metals and nutrients in rainwater in coastal regions between the southern Yellow Sea and East China Sea

Rainwater samples were collected in series in Qianliyan Island (southern Yellow Sea) and Shengsi Archipelago (East China Sea) between May 2000 and May 2002, chemical analysis for pH values,concentrations of heavy metals (Cu, Pb, Zn and Cd) and nutrients (NH4^-, NO3^-, PO43^-, SiO32^-) were performed.Results indicate that concentrations of most of the heavy metals and nutrients in rainwater show clear seasonal variation, i.e. high level in winter and low level in summer. Regionally, concentrations are higher in the southern Yellow Sea than in the East China Sea, but the annual input of heavy metals into oceans by wet deposition is similar in both stations. However, the input of nutrients by wet deposition in the East China Sea is 2-3 times higher than that in the southern Yellow Sea. In individual, Pb and PO4^3- are input to the sea mainly by dry deposition; whereas Cu, Zn, Cd and N compounds are input dominantly by wet deposition, the N/P ratios in the rainwater from two stations are much higher than those in seawater, showing a significant impact of atmospheric wet deposition on marine production and biogeochemical circulation of nutrients in these sea regions.     

Antifouling effect of bioactive compounds from selected marine organisms in the Obhur Creek,Red Sea

Three species of sponges and a tunicate were collected from Obhur creek of Jeddah coast for this bioactivity study. In order to assess the antifouling efficacy of selected marine organisms, methanolic extracts of these organisms were tested against different fouling bacterial forms and II-instar stage of the barnacle, Balanus amphitrite. Antibiosis, bioactivity and followed by multivariate analyses were carried out to check the efficacy of antifouling effect of the selected marine organisms. Principal component analysis revealed the exemplary antifouling efficacy of the sponge extracts of Stylissa sp. observed followed by Hyrtios sp. against bacterial forms in the laboratory study. De-trended correspondence analysis confirmed that the contribution of antifouling efficacy of the selected sponge extracts was observed to be more towards Bacillus sp., Vibrio sp. and Alteromonas sp. Moreover, the efficacy of Hyrtios sp. extract(20.430 μg m L~(-1)) followed by Stylissa sp.(30.945 μg m L~(-1)) showed higher against barnacle instar compared with other extracts in the bioactivity assay. Bray-Curtis cluster analysis under paired linkage categorized all the sponge extracts into one major cluster with 75% similarity, and one outlier tunicate. More than 80% similarity observed between Hyrtios sp. and Stylissa sp. Fourier transform infrared spectroscopy(FTIR) showed that the contribution of major peaks found in the marine organisms were towards sulfones, sulfoxides, cyanates and ketones.     

Toxic effects of Zn2+, Cu2+, Cd2+ and NH3 on Chinese prawn

This study deals with the toxic effects of Zn²⁺, Cu²⁺, Cd²⁺ and NH₃ on Chinese prawn (Penaeus orientalis). The median lethal concentrations of the toxic matters on Chinese prawn were determined. Cd²⁺>Cu²⁺>Zn²⁺>NH₃-Nm is the toxic order of these chemicals to Chinese prawn. The toxic order of concentrated Zn²⁺ and Cd²⁺ in tissues and organs of Chinese prawn is in the order. gill>shell+appendage>viscera>muscle.     

A polysaccharide-degrading marine bacterium Flammeovirga sp. MY04 and its extracellular agarase system

Bacteria of the genus Flammeovirga can digest complex polysaccharides (CPs), but no details have been reported regarding the CP depolymerases of these bacteria. MY04, an agarolytic marine bacterium isolated from coastal sediments, has been identified as a new member of the genus Flammeovirga. The MY04 strain is able to utilize multiple CPs as a sole carbon source and grows well on agarose, mannan, or xylan. This strain produces high concentrations of extracellular proteins (490 mg L-1 ± 18.2 mg L-1 liquid culture) that exhibit efficient and extensive degradation activities on various polysaccharides, especially agarose. These proteins have an activity of 310 U mg-1 ± 9.6 U mg-1 proteins. The extracellular agarase system (EAS) in the crude extracellular enzymes contains at least four agarose depolymerases, which are with molecular masses of approximately 30-70 kDa. The EAS is stable at a wide range of pH values (6.0-11.0), temperatures (0-50℃), and sodium chloride (NaCl) concentrations (0- 0.9 mol L-1). Two major degradation products generated from agarose by the EAS are identified to be neoagarotetraose and neoagarohexaose, suggesting that β-agarases are the major constituents of the MY04 EAS. These results suggest that the Flammeovirga strain MY04 and its polysac-charide-degradation system hold great promise in industrial applications.