Distribution of Deep-Sea Benthos in the Proposed Mining Area of Central Indian Basin

During the first stage of the project work on the Indian Deep-Sea Environment Experiment (INDEX), the abundance and distribution of deep-sea benthos were surveyed in the Central Indian Basin for the collection of baseline data. The deep-sea community of the sediment was characterized by a moderately high standing crop and diverse fauna. The macrofaunal component was dominated by polychaetes (100% prevalence) and peracarid crustaceans, whereas the meiofauna was dominated by nematodes and harpacticoid copepods. The results of this study conform to the general distribution reported elsewhere. The macrofaunal abundance showed an inverse relation to the abundance of polymetallic nodules. However, the relation between meiofaunal vertical distribution and the vertical profile of the total organic matter and total labile matter was positive.     

Response of Meiofauna to Immediate Benthic Disturbance in the Central Indian Ocean Basin

Abstract

Twenty-four stations (12 each, during pre- and postdisturbance studies) 5300 to 5330 m deep between 10°01' and 10°03'S latitude and between 75°59' and 76°02'E longitude were sampled to study the effect of benthic disturbance on the distribution of meiofauna in the Central Indian Ocean. Bottom-sampling was conducted with a box corer. Total meiofauna density ranged from 35 to 45 organisms per 10 cm² of bottom area during the predisturbance period and 21 to 32 organisms per 10 cm² during the postdisturbance period in the test and reference sites. Differences between pre- and postdisturbance study results were statistically significant (P < 0.05). Nematoda was the most abundant meta-zoan group, on an average representing >55% of the meiofaunal population. The abundance of nematodes and harpacticoid copepods as well as total meiofauna showed marked decreases during postdisturbance sampling. Vertical distribution of meiofauna in the sediment cores revealed that 75% to 90% of the metazoan population was confined to the top 2-cm layer of the sediment. Recolonization experiments suggest that harpacticoid copepods may take more time for recolonization than the nematodes and are more sensitive to the physical disturbance. These findings suggest that nematodes and harpacticoid copepods can be used as indicator organisms in recolonization experiments.     

胶州湾海域大型底栖生物的调查与研究

根据2003年6月初对胶州湾海域的调查资料,分析了该海域的底栖生物的种类组成及生物量分布。共鉴定了40种底栖生物,平均总生物量为23.93g/m2,平均栖息密度为400个/m2。调查结果表明,生物量受盐度影响,而栖息密度受水深和有机质含量影响。胶州湾由于受污染缘故,多样性指数远低于3,生物群落呈非健康状态,并与20世纪90年代的调查结果相比较发现,生物量呈严重下降趋势,而栖息密度呈上升趋势。     

Restoration of Deep-Sea Macrofauna after Simulated Benthic Disturbance in the Central Indian Basin

ABSTRACT

Macrofaunal communities of the Central Indian Basin (CIB) were sampled with a spade before (June 1997), and immediately after (August 1997), and 44 months (April 2001) after a simulated benthic disturbance for polymetallic nodule mining. The average density recorded down to a sediment depth of 40 cm ranged from 89 to 799 ind·m^?2 (mean: 373 ± 221 SD; n = 12) and 178–1066 ind·m^?2 (mean: 507 ± 489 SD; n = 3) in the test and reference area, respectively. Most of the macrobenthic animals (64%) were concentrated in the upper 0 to 2 cm sediment layers, whereas, sizeable fauna (6%) inhabited the 20–40 cm sediment section and the deepest 5 cm section from 35–40 cm contributed only about 2% to the total population density. The fauna, comprised of 12 groups, were dominated by the nematodes, which constituted 54% of the total population. The macrofaunal density in the test site showed a significant increase (x:400 ind·m^?2) in the 44 months postdisturbance sampling (x:320 ind·m^?2). The population of nematodes and oligochaetes was nearly restored after 44 months, but the polychaetes and crustaceans did not reach the baseline populations measured in June 1997. The top 0–2 cm sediment layer was severely affected by the disturber, and the study suggests that physically disturbed deep-sea macrofauna may require a longer period for restoration and resettlement than normally believed.     

Restoration of Deep-Sea Macrofauna after Simulated Benthic Disturbance in the Central Indian Basin

Macrofaunal communities of the Central Indian Basin (CIB) were sampled with a spade before (June 1997), and immediately after (August 1997), and 44 months (April 2001) after a simulated benthic disturbance for polymetallic nodule mining. The average density recorded down to a sediment depth of 40 cm ranged from 89 to 799 ind·m^-2 (mean: 373 ± 221 SD; n = 12) and 178-1066 ind·m^-2 (mean: 507 ± 489 SD; n = 3) in the test and reference area, respectively. Most of the macrobenthic animals (64%) were concentrated in the upper 0 to 2 cm sediment layers, whereas, sizeable fauna (6%) inhabited the 20-40 cm sediment section and the deepest 5 cm section from 35-40 cm contributed only about 2% to the total population density. The fauna, comprised of 12 groups, were dominated by the nematodes, which constituted 54% of the total population. The macrofaunal density in the test site showed a significant increase (x:400 ind·m^-2) in the 44 months postdisturbance sampling (x:320 ind·m^-2). The population of nematodes and oligochaetes was nearly restored after 44 months, but the polychaetes and crustaceans did not reach the baseline populations measured in June 1997. The top 0-2 cm sediment layer was severely affected by the disturber, and the study suggests that physically disturbed deep-sea macrofauna may require a longer period for restoration and resettlement than normally believed.     

印度洋深海沉积物可培养放线菌分离样品预处理条件的优化

海洋沉积物微生物资源丰富,放线菌是其中的优势类群之一,但是海洋放线菌纯培养难度较大.本文利用响应面法对采自印度洋的深海沉积物样品进行湿热处理条件优化,实验中选取了5个因素设计实验,生长的放线菌经纯化培养后,针对16S rRNA基因进行测序分析,以每种优化条件下培养出的放线菌种类数为响应值,优化结果最终为:湿热处理温度5...     

Benthic Metabolism and Standing Stock in the Central and Northern Deep Red Sea

Abstract. Benthic metabolism and standing stocks were investigated in the deep Red Sea between 21^o and 27^oN, Activity was assessed by the determination of respiration rates with a shipboard method and by calculating oxygen consumption from the activity in the electron transport system. We attempted to compare results from different latitudes within the warm Red Sea and with data from cold Atlantic environments. Our investigations were part of an environmental risk assessment to evaluate future mining of metalliferous sediments from the Atlantis II Deep.     

Microbial Biomass and Organic Nutrients in the Deep-Sea Sediments of the Central Indian Ocean Basin

In order to assess the impact of deep-sea mining on the in situ benthic life, we measured the microbial standing stock and concentration of organic nutrients in the deep-sea sediments of the Central Indian Ocean Basin in the Indian pioneer area. Sediments were collected using box core and grab samples during September 1996. The total bacterial numbers ranged from 10 10 -10 11 cells per g -1 dry weight sediment. There was a marginal decrease in the number of bacteria from surface to 30 cm depth, though the subsurface section registered a higher number than did the surface. The highest numbers were encountered at depths of 4-8 cm. The retrievable number of bacteria were two orders less in comparison with the direct total counts of bacteria. An almost homogeneous distribution of bacteria, total organic carbon, living biomass, and lipids throughout the depth of cores indicates active microbial and benthic processes in the deep sea sediments. On the other hand, a uniform distribution of total counts of bacteria, carbohydrates, and total organic carbon in all the cores indicates their stable nature and suggests that they can serve as useful parameters for long-term monitoring of the area after the benthic disturbance. Further studies on temporal variability in this region would not only verify the observed norms of distribution of these variables but would also help to understand restabilization processes after the simulated benthic disturbance.     

Monitoring the Impact of Simulated Deep-sea Mining in Central Indian Basin

Monitoring of deep-sea disturbances, naturai or man-made, has gained significance due to the associated sediment transport and for the ensuing alterations in environmental conditions. During the Indian Deep-sea Environment Experiment (INDEX), resuspension of deep-sea sediment in the Central Indian Basin (CIB) resulted in an increase and lateral movement of suspended particles, vertical mixing of sediments, changes in sedimentological, biochemical, and geochemical conditions and an overall reduction in benthic biomass. Monitoring the conditions 44 months after the experiment has shown a partial recovery of the benthic ecosystem, with indications of restoration and recolonization.     

Distribution and Origin of Seamounts in the Central Indian Ocean Basin

Approximately 200 seamounts of different dimensions have been identified, from multibeam bathymetry maps of the Central Indian Ocean Basin (CIOB) (9°S to 16°S and 72°E to 80°E), of which 61% form eight chains that trend N-S. The seamounts are clustered above and below 12°S latitude. Area II (9°-12°S) shows a concentration of smaller seamounts (≤400 m height), and area I (12°-15°S) has a mixed population (including both less and more than 400 m height). Inspite of the differences in their height, the seamounts of these eight chains are morphologically (slope angle, flatness, basal width) corelatable. Furthermore, we suggest that height-width ratio could be useful in identifying the style of seamount eruption. The seamount chains in the CIOB probably originated from propagative fractures and were produced between 61 and 52 Ma (chrons A26 to A23) as a result of the interaction between the conjugate crusts of the Central Indian and Southeast Indian Ridges during the Indo-Eurasian collision event.     

Distribution and Origin of Seamounts in the Central Indian Ocean Basin

Approximately 200 seamounts of different dimensions have been identified, from multibeam bathymetry maps of the Central Indian Ocean Basin (CIOB) (9°S to 16°S and 72°E to 80°E), of which 61% form eight chains that trend N-S. The seamounts are clustered above and below 12°S latitude. Area II (9°–12°S) shows a concentration of smaller seamounts (≤400 m height), and area I (12°–15°S) has a mixed population (including both less and more than 400 m height). Inspite of the differences in their height, the seamounts of these eight chains are morphologically (slope angle, flatness, basal width) corelatable. Furthermore, we suggest that height-width ratio could be useful in identifying the style of seamount eruption. The seamount chains in the CIOB probably originated from propagative fractures and were produced between 61 and 52 Ma (chrons A26 to A23) as a result of the interaction between the conjugate crusts of the Central Indian and Southeast Indian Ridges during the Indo-Eurasian collision event.     

Distribution of Grain Size and Clay Minerals in Sediments from the INDEX Area, Central Indian Basin

The granulometry of 21 box core sediments from five selected sites in the Central Indian Basin (CIB) have been studied to understand the sediment size characteristics in the Pioneer area before the benthic disturbance experiment. The sediments are predominantly clayey silt. Statistical parameters were determined to understand the control and variations in the grain size. The mean grain size (M z φ) ranged from 7.0 to 8.6 for the surface and from 6.6 to 8.6 for the subsurface sediments, indicating deposition of very fine fractions during the Neogene period. The sediments were poorly sorted as revealed from the higher σI values (2.7 to 2.9). Higher and negative values of skewness (SK I ) indicated a winnowing action of prevailing currents in the area. Average kurtosis (K G ) values for the surface (0.4 to 0.8) and subsurface (0.5 to 1.2) showed that sediments were very platykurtic and leptokurtic. Clay mineralogical studies showed that smectite had a wider range (from 6% to 55%) and decreased southward because of its terrigenous origin. The terrigenous influence of the sediments could be documented as far as 12°S. Smectite and kaolinite increased eastward and chlorite decreased southward. This observation positively correlated with the mean current direction in the area. However, the influence of Antarctic Bottom Water (AABW) in the area could be predicted from the southward increase in chlorite content.     

Distribution of Grain Size and Clay Minerals in Sediments from the INDEX Area,Central Indian Basin

Abstract

The granulometry of 21 box core sediments from five selected sites in the Central Indian Basin (CIB) have been studied to understand the sediment size characteristics in the Pioneer area before the benthic disturbance experiment. The sediments are predominantly clayey silt. Statistical parameters were determined to understand the control and variations in the grain size. The mean grain size (M_zφ) ranged from 7.0 to 8.6 for the surface and from 6.6 to 8.6 for the subsurface sediments, indicating deposition of very fine fractions during the Neogene period. The sediments were poorly sorted as revealed from the higher ρ₁ values (2.7 to 2.9). Higher and negative values of skewness (SK₁) indicated a winnowing action of prevailing currents in the area. Average kurtosis (K_G) values for the surface (0.4 to 0.8) and subsurface (0.5 to 1.2) showed that sediments were very platykurtic and leptokurtic. Clay mineralogical studies showed that smectite had a wider range (from 6% to 55%) and decreased southward because of its terrigenous origin. The terrigenous influence of the sediments could be documented as far as 12°S. Smectite and kaolinite increased eastward and chlorite decreased southward. This observation positively correlated with the mean current direction in the area. However, the influence of Antarctic Bottom Water (AABW) in the area could be predicted from the southward increase in chlorite content.     

Quantitative Distribution of Deep-water Benthos in Western Central Pacific

Altogether 10 surfaces and 8 cores were sampled quantitatively from the Western Central Pacific(4°00′S—5°00′N, 160°00′—165°00′E and 2°00′—10°00′S, 170°00′—173°20′E) in 1978 and 1979. The results of preliminary analysis show that the faunal composition was quite simlpe, only eight species of macrobenthic invertebrates, four species of Polychaete, two     

Regional Variability in Occurrence and Distribution of Polymetallic Nodules in the Central Indian Basin

Regional scale morphological studies on different-sized polymetallic nodules from the Central Indian Basin show that only 2–5 cm sized nodules have highest occurrence per station (92–94% along latitudes; 88–94% along longitudes). A nodule size shows an inverse relationship with the nodule distribution, grade, and abundance. Interestingly, only 3–4 and 4–5 cm sized nodules have highest grade, abundance, and distribution percentages along longitudes in P₁, first generation mine site and relinquished area, which further implies that the nodule mining along the longitude would be beneficial. Different-sized nodule groups suggest their formation under variable environmental conditions that existed in the Central Indian Basin. The coexistence of different-sized nodules suggests different environmental conditions existed in the Central Indian Basin.     

Changes in Irish Sea Benthos: Possible Effects of 40 years of Dredging

From 1946 to 1951 Dr N. S. Jones sampled the benthos around the south of the Isle of Man from over 200 sites. Multivariate methods have been used here to compare subsets of this historical data with recent data from the same locations: of these locations some have been subject to heavy scallop dredging over the intervening 40 plus years and some to little dredging. Clear changes were apparent regardless of scallop dredging intensity. Some of the changes in the heavily dredged areas were those expected to result from extreme physical disturbance—an increased polychaete mollusc ratio, loss of some fragile species, and an increase in the predominance of scavenger/predator species. However, changes in the lightly dredged areas also included the loss of a number of species including some potentially fragile tube-dwellers. Reasons for these changes were not apparent.     

Distribution pattern and morphochemical relationships of manganese nodules from the Central Indian Basin

In the Central Indian Basin manganese nodule abundance was variable in all sediment types. Mean abundance varied from 1.5 in calcareous ooze to 10.2 kg/m² in terrigenous-siliceous ooze sediments. Nodule grade and growth rates are positively correlated only up to 10 mm/My (million years), and grade shows no distinct relationship with abundance. Relationships between the morphochemical characteristics of the nodules and host sediment types are subtle. Both hydrogenetic and diagenetic nodules (with smooth and rough surfaces respectively) occur on almost all sediments, but in variable proportions. Thus, the overall distribution pattern shows that small nodules (<4-cm diameters) of lower grade (average value Ni+Cu+Co=1.21%) with smooth surfaces are more common on red clay, terrigenous, and terrigenous-siliceous ooze transition-zone sediments. By contrast, large nodules (>4-cm diameters) of higher grade (average value Ni+Cu+Co=1.80%) with rough surfaces are more prevalent on siliceous ooze, siliceous ooze-red clay, and calcareous ooze-red clay transition-zone sediments. This implies an enhanced supply of trace metals from pore waters to rough-surface nodules during early diagenesis.     

Deep-Sea Eukaryote Ecology of the Semi-Isolated Basins Off Japan

The Japanese archipelago is surrounded by the Pacific to the east, the Okhotsk Sea to the north, the Sea of Japan to the west and the Okinawa Trough to the south. The last three seas form semi-isolated deep basins, all with potentially tectonic origin but a different primary energy source as well as hydrographic and faunistic history. The Okhotsk Sea is connected to the Pacific through the deep straits between the Kurile Islands. As a result much of the fauna has links with that fauna found at similar depths in the Pacific. By contrast, the Sea of Japan was isolated from the main Pacific during the last ice age and became anoxic. Even today the link is only through narrow shallow straits. As a result the fauna is impoverished and is believed to be composed of cold-adapted eurybathic species rather than true deep-sea species. The deep-water fauna of both these seas derive their energy from sinking surface primary production. The Okinawa Trough has a much younger tectonic history than the Okhotsk Sea or the Sea of Japan. In the Okinawa Trough the most noticeable fauna is associated with hydrothermal activity and chemosynthesis forms the base of the food chain for the bathyal community. The variable nature of these three basins offers excellent opportunities for comparative studies of species diversity, biomass and production in relation to their ambient environment. This revised version was published online in August 2006 with corrections to the Cover Date.     

Bathymetric patterns of deep-sea gastropod species diversity in 10 basins of the Atlantic Ocean and Norwegian Sea

Bathymetric patterns of macrofaunal species diversity are best documented in the western North Atlantic where diversity is a unimodal function of depth, peaking in the mid-bathyal zone and being depressed in the upper slope and abyss. There are few inter-basin studies of diversity-depth trends that are controlled for taxonomy, sampling gear, and diversity measures. In this paper, we compare gastropod diversity gradients in the North American Basin of the Atlantic to estimates of diversity in 9 other regions: the Norwegian Sea, West European Basin, Guiana Basin, Gambia Basin, Equatorial Mid-Atlantic, Brazil Basin, Angola Basin, Cape Basin and Argentine Basin. All samples were collected with epibenthic sleds, and diversity calculated by the Sanders-Hurlbert normalized expected number of species. While sampling in other regions is generally less complete than in the western North Atlantic, results indicate that a unimodal pattern is not universal. Diversity can increase, decrease or show no relationship with depth. The level of diversity also varies among basins relative to the western North Atlantic, being depressed in the Norwegian Sea, at bathyal depths in the eastern North Atlantic, and below an oxygen minimum zone in the Cape Basin, and generally elevated at tropical latitudes and in abyssal regions where food supply is high. Associations between gastropod diversity and the ecology and geology of basins suggest that productivity, oxygen concentration, hydrographic disturbance and evolutionary-historical processes may be implicated in shaping bathymetric diversity gradients, but specific causes are difficult to discern. Much more intensive sampling, analyses of other major taxa, and more detailed ecological data are necessary to understand deep-sea biogeography at within- and between-basin spatial scales.     

Fungi in deep-sea sediments of the Central Indian Basin

Although a great amount of information is available on bacteria inhabiting deep-sea sediments, the occurrence of fungi in this environment has been poorly studied and documented. We report here the occurrence of fungi in deep-sea sediments from ∼5000 m depth in the Central Indian Basin (9–16°S and 73–76°E). A total of 181 cultures of fungi, most of which belong to terrestrial sporulating species, were isolated by a variety of isolation techniques. Species of Aspergillus and non-sporulating fungi were the most common. Several yeasts were also isolated. Maximum species diversity was observed in 0–2 cm sections of the sediment cores. Direct staining of the sediments with Calcofluor, a fluorescent optical brightener, revealed the presence of fungal hyphae in the sediments. Immunofluorescence using polyclonal antibodies raised against a deep-sea isolate of Aspergillus terreus (# A 4634) confirmed its presence in the form of hyphae in the sub-section from which it was isolated. A total of 25 representative species of fungi produced substantial biomass at 200 bar pressure at 30° as well as at 5 °C. Many fungi showed abnormal morphology at 200 bar/5 °C. A comparison of terrestrial isolates with several deep-sea isolates indicated that the former could grow at 200 bar pressure when growth was initiated with mycelial inocula. However, spores of a deep-sea isolate A. terreus (# A 4634), but not the terrestrial ones, showed germination at 200 bar pressure and 30 °C. Our results suggest that terrestrial species of fungi transported to the deep sea are initially stressed but may gradually adapt themselves for growth under these conditions.