Prélèvements systématiques et caractérisation des réflecteurs acoustiques: nouvelle étape dans la compréhension de la géologie de la Méditerranée occidentale

A sampling survey in the Ligurian Sea and north of the Balearic Islands (Mediterranean Sea) brought back samples containing the following elements: (1) lithified Quaternary crust and deep-lying corals; (2) Early Pliocene marls corresponding to reflector level M in some cases; (3) red sediments tentatively assimilated with the Messinian continental equivalent; (4) rocks coming from the basement; (5) volcanic rocks.For the first time, basalts with olivine were discovered in the Algero-Provencal Abyssal Plain.A volcano 500 m high was sampled by dredging north of Minorca.     

Influence de la circulation à long terme sur la répartition des organismes zooplanctoniques dans la Baie de Campeche,Mexique

A space-time approach is adopted in an attempt to understand the long-term influence of currents on the spatial pattern distribution of zooplankton, ichthyoplankton, and fish larvae communities in the Bay of Campeche, Mexico.The analysis is performed using results of a hydrodynamic numerical model, and zooplankton and ichthyoplankton abundances.Three fish larvae communities were identified in the Bay, on the basis of its physiography, i.e.: “neritic fluvio lagoon influence”, “neritic” and “oceanic” communities.A cyclonic eddy is formed in the Bay and drifts westward, facilitating transport and distribution of planktonic organisms. The eddy provides larvae transport from oceanic areas to the coastal areas and vice-versa. The seasonal distribution of fish larvae is highly influenced by the position of the eddy.The biomass pattern and fish larvae density observed in the coastal area off the Grijalva-Usumacinta delta are governed by the coastal front.     

Phases d'érosion—comblement de la vallée de la Papenoo et volcanisme subrécent à Tahiti,en relation avec l'évolution des iles de la Société (Pacifique Sud)

A detailed mapping and data from borings support the conclusion that there is a late lava flow inside the Papenoo valley, the main valley of Tahiti island. This last volcanic manifestation which took place 400,000 years ago came after the phase of erosion corresponding to the Illinoian glacial period. It is one of the four phases of erosion followed by filling observed in the cross section of the Papenoo valley.     

Méthodologie pour une simulation des transformations granulométriques de sables de la zone d'avant-côte

The grain size of mature coastal sands changes rapidly in response to the variation of the energy regime which provokes either erosion or deposition of fractions of the textural assemblage. Whatever the dynamic conditions, questions of paramount importance such as the size of grain populations transported simultaneously, the most common size of the grains in movement and the grain-size range of the sediment fraction involved are still poorly understood. The present paper deals with an attempt to describe this fraction without any consideration of the processes responsible for the observed changes. A major problem results from the fact that the distribution of the mobilized fraction cannot be known and described by direct comparison between the initial and the final grain-size curves. The only way to characterize the mobile fraction between two subsequent grain-size states is to simulate various kinds of removal or deposition. The first question is to express the shape of the actual distributions of the sediments mathematically. The simple Gaussian approximation being irrelevant, distributions can be expressed using advanced computer programmes which can provide mathematical expressions for all distributions. The cubic-spline approximation was chosen. The mobile fraction was assumed to have a Gaussian distribution. Various tests were made in order to simulate qualitative phenomena observed in nature (bimodality, grain-size parameter changes…) and to assess the three parameters which define the characteristics of the fraction deposited or removed: modal value, dispersion of the mobile population and relative amplitude of the change. An application was carried out on the Senegalese coast offshore from the Senegal delta and on the Mediteranean coast in the Gulf of Lions. It suggests that the dynamic agents have a great selectivity. Further developments are considered.     

Survie et maintien de la virulence de Salmonella Typhimurium VNC exposée simultanément à trois facteurs stressants expérimentaux

The release of enteric pathogenic bacteria in aquatic environments poses the problem of the fate of these bacteria under the effects of environmental factors (solar radiation, salt concentration, temperature, nutrient level, pH, competition). Frequently, these bacterial cells, potentially pathogenic, enter into a non-culturable state on routine bacteriological plating media. However, the use of direct detection methods DAPI stained cells allows the visualization of these Viable but Non Culturable cells VNC. But, beyond the characterization of the viability of the cells electron transport activity, metabolic activity, membrane integrity, structure and/or quantity of DNA, what happens with the virulence of these cells? This problem was experimentally investigated according to the bacterial model Salmonella Typhimurium. The virulence of this strain, which is the agent of the murine typhoid, was evaluated on a mouse model. Experimentally, the effects of some environmental factors on the survival and on the maintenance of virulence of Salmonella Typhimurium were measured in microcosms exposed to UV radiation (four germicidal lamps 8 mW s⁻¹ cm⁻², wave length: 254 nm, salt concentration (Sea Salt Sigma, 37, nutrient starvation. The microcosms were simultaneously submitted to these three factors, with variable exposure times. For each of those times, the viability of the nonculturable cells which became nonculturable because of the exposure to the three factors was measured through different physiological states notable in the cells, after using different fluorescent dyes. The stained cells were observed by epifluorescence microscopy and analysed by image cytometry. So, the cellular populations are characterised by enumeration of respiring bacteria CTC, [39], metabolising bacteria YEK, [22] modified, bacteria owning an undamaged cytoplasmic membrane LD, Live/Dead BacLight Viability Kit. Molecular Probes Inc.; we also determined the quantity and/or structure of DNA of the cells fluorescence level of DAPI stained cells), After exposure to the three factors for one hour 13.56 J cm⁻², while the plate count cell density rapidly decreased from #10⁷ cells mL⁻¹ to0.1 cell mL⁻¹, physiological states of these viable but non-culturable cells are similar to those of non-exposed cells. On the other hand, after exposure for three hours, only 10 % of the cells deposit a CTC formazan-crystal and 20 % are substrate responsive cells enlarged cells in presence of Yeast Extract and Cephalexin: YEC. Half of the cellular population presents an undamaged cytoplasmic membrane and the level of fluorecence of DAPI stained cells is close to 85 %, which shows that the DNA of these cells is weakly damaged. After exposure to the three experimental factors for24 hours 315 J cm⁻², weak replies to the physiological tests used in this study to characterize the viability of the non-culturable cellular population are observed CTC: 4 %; YEC: 2 %; LD: 11.8 % while the fluorescence level of DAPI stained cells remains firm at 80 %. At the same time, the virulence expression of VNC cells of Salmonella Typhimurium, evaluated by intraperitoneal injection to the mouse route which excludes uncontrolled parameters, unlike the per os route does not seem to be correlated with the cellular viability such as it has been evaluated in this study. A 30 min exposure (6.73 J cm⁻²) to the three environmental factors, leading to the non-culturability of almost the entire exposed cell population 0.08 culturable cell mL⁻¹ whereas the level of viability of those culturable cells is closed to the one of non-exposed cells. The injection of 1000 of those cells 0.001 culturable cells in 100 μL inoculated into the mouse a group of ten mice does not cause any mortality four weeks post-inoculation, whereas the injection of the same dose of non-exposed cells leads to the death of all mice in the group one week post-inoculation. According to our preliminary experiments on Salmonella Typhimurium, the loss of the state of culturability and the loss of virulence towards mice by intra-peritoneal route, because of the exposure to associated effects of UV irradiation 254 nm, salinity 37 and nutrient starvation, seem to be concomitant.     

Structures géologiques de la marge continentale à l'ouest du Spitzberg

Résumé L'étude de plusieurs profils de sismique réflexion, magnétisme et gravimétrie a permis de dégager les grands traits de la structure de la marge continentale à l'ouest du Spitzberg. Cette marge est représentée au niveau du talus continental par un bassin sédimentaire dont la largeur se réduit en allant vers le Nord. Ce bassin est limité à l'Ouest par les premiers contreforts de la dorsale d'Atka qui a joué un rôle de barrage pour les apports sédimentaires. Les données de la sismique réflexion et du magnétisme indiquent que la vallée d'Atka est sans doute une zone actuelle d'expansion. Ces résultats sont en bon accord avec le modèle récent d'ouverture de la mer de Norvège. De plus, la dorsale d'Atka semble avoir subi au pliocène un réajustement ayant modifié les processus sédimentaires au niveau de la marge.

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The main structural features of the continental margin off West Spitzberg are outlined. Seaward the Atka ridge has acted like a dam for the eastward-flowing sediments and thus helped to build up a large sedimentary basin, narrowing towards the North. Magnetic and seismic data show that the Atka Valley is probably an active accretion rift valley. These results are in good agreement with the last hypothesis on the opening of the Norwegian Sea. During the Pliocene, a tectonic event may have affected Atka ridge and modified the subsequent sedimentation.

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Contribution No. 258 du Department Scientifique du Centre Océanologique de Bretagne.     

Absorption et régénération de l'azote dans le système brassé de la Manche: productions nouvelle et régénérée

Nitrate and ammonium uptake rates were measured in spring and summer in deep and shallow wellmixed waters of the English Channel during different cruises between 1986 and 1994. In the deep waters, nitrate uptake was relatively low during phytoplankton development and ammonium uptake represented more than 70 % of the total uptake irrespective of the season. In the shallow waters, nitrate uptake during spring phytoplankton growth was high and represented about 75 % of the total uptake. Ammonium uptake became substantial towards the end of spring and summer. The high contribution of ammonium to the nitrogenous nutrition of phytoplankton over the whole of the well-mixed waters is related to a high rate of recycling of nitrogen in the water column. Ammonium regeneration by microheterotrophs can satisfy between 62 % and the totality of the phytoplankton nitrogen requirements. The high primary production in deep well-mixed waters is not supported by allochthonous nitrogen supply but by an intense in situ regeneration of nitrogen. The situation is similar in shallow well-mixed waters, except during the spring bloom.     

Biodiversité et dynamique des peuplements ichtyiques de la mangrove de Guaratuba,Brésil

The Bay of Guaratuba, whose area is approximately 45 km², is a basin, situated to the south of Brazil (25° 52′ S; 48° 39′ W) and connected to the Atlantic ocean. In its northern part, a region of mangrove is found, under the influence of seasonal fluvial contributions, dominated by Laguncularia racemosa. The depth exceeds 6 m in some places. According to the period of the year, salinity varies between 3 and 37, and temperature between 15 and 28 °C. Several of the present fish species are exploited by commercial and recreational fisheries. This work describes characteristics of the fish populations occupying this mangrove, and temporal and spatial evolution of populations of the main species from available data on their biology. Between September 1993 and August 1996, fish samplings have been undertaken monthly by trawl, according to the same fishing effort (20 min), in channels of this ecosystem. Size, numerical abundance and weight of each individual of species were measured, as well as surface and bottom temperatures and bottom salinity. Species richness, Shannon-Weaver's diversity index were calculated and abundances were processed by the multivaried analysis. The global species richness is 61 species, distributed in 28 families; the better represented species are Sciaenidae (10), Gerreidae (5), Carangidae (5), Ariidae (4), Haemulidae (3), Serranidae (3), Bothidae (3). Each year, the number of species varies between 41 and 44, and a group of eight species represents between 80 to 90 % of the density and 75 % of the biomass. The constancy of this dominance is the main characteristic of this fish assemblage. The most represented species in abundance is Pomadasys corvinaeformis is the first two years, then Stellifer rastrifer. The group of eight main species is shared by 15 species in total, of which 13 for the density and 14 for the biomass. The most important monthly catches take place in winter season, during which one observes a decrease of water temperature and an increase of salinity. These species, however, being likely to be replaced by others according to the year or the season; one never finds the same composition in the assemblage which preserves always the same structures. Seasonal differences between seasons under marine (winter) and continental (summer) influences play a major role on the dynamics of fish in the Guaratuba mangrove. This ecosystem plays a functional role in the achievement of the life cycle of many permanent or temporary species. Those having succeeded to reach high levels of abundance are not represented in a uniform way in the course of the year, even when they reside permanently in the ecosystem. Periodic displacement strategies for self regulation of stocks abundance, are necessary mechanisms for these populations, which thus success to coexist in occupying the mangrove.     

Données géomorphologiques sur la région de Fresh Creek,Ile Andros (Bahama)

A geomorphological study of the east coast of Andros (Fresh Creek area) shows the existence of a paleotopography represented by low-altitude hills (few metres). This paleotopography is protected by the presence of a calcitic Quaternary crust which covers Pleistocene calcarenite.In the western part of the area, there are long woody axes, oriented NE-SW, parallel to the channels of the creek. They end at two kilometres from the coast, along which is a second kind of lower hills, orthogonal to the first.The first axes can be interpreted as megaripples as seen at the present time on modern deposits (on the Great Bahama Bank) and fossilized by the upper crust. The second direction is made by accretion ripples along the coast.The surface of the Bahamian calcarenite has been studied. The Bahamian karst presents two topographical forms: “blue holes” like those outside the island, which are 60–80 m in diameter and both sparse and deep; and “washtub” dolines; these are numerous and shallow, and, from low altitude, exhibit a honeycombed aspect on the surface. This karstic topography with dolines and blue holes is also seen through the water of the Creek the hard bottom of which is covered only here and there with a few centimetres of sediments. Hence, there is a submerged karstic topography, made of the same elements as the aerial karst, but submerged by the Holocene transgression. The present karstic relief, in relation with the different eustatic levels of the Quaternary, has begun 120,000 years ago, according to the isotopic ages, and might be composed by different steps, difficult to show now, in the topography.The blue holes in the interior of the island of young and little evolved karst, were formed more by solution than by collapse of the karstic caves, because of the absence of a real river to drain the Andros shelf at the time of low sea levels. Blue holes of the inside of the island, as they are called, with submarine openings, have the same salinity as the water of the creek (17.5 g/l). The dolines with very low salinity (0.7 g/l to 3.8 g/l) are filled with stromatolites and charophytes, slowly forming sediments made up essentially of high-magnesian calcite.It seems that the Andros Island karst can be compared with that of the Yucatan, where there are round and deep open pits, called cenote, of which the Bahamian equivalent would be the blue holes which were drowned by the Holocene transgression.     

Comparaison de méthodes d'évaluation de la production secondaire d'une population de l'annélide polychète Ditrupa arietina (O.F. Muller)

We compared the ability of three direct methods (increment summation, loss summation and instantaneous growth rate coefficient), the size-frequency method, and eight indirect methods (Banse and Mosher, Benke, Brey, Edgar, Morin and Bourassa, Plante and Downing, Robertson and Schwinghamer et al.) to measure or to predict the secondary production of a Mediterranean population of the polychaete Ditrupa arietina. This comparison was carried out both on the 1994 cohort during its two-year lifespan and on the entire population during the two years of the study (1994–1996). Our results showed that all three direct methods are more or less equivalent. Results referring to the year 1994–1995 showed a strong overestimation of the actual production by the size frequency method. This is attributed to the existence of an average individual maximal size below which the actual production equals zero. During the same year, the results obtained using indirect methods were highly variable, partly, but not solely, due to the type of environment for which the regression models were built. The incorporation of temperature as an independent variable within the most recent regression models did not contribute to improving the quality of the predictions. Results referring to the year 1995–1996 confirm the incapacity of both the size frequency and the indirect methods to predict changes in the P/B ratio in relation with age structure and recruitment irregularities. These results are discussed in the context of inferring productivities both at the population and the community level.     

Variabilité du panache turbide de la Gironde par télédétection. Effets des facteurs climatiques

The Gironde estuary (France) discharges to the ocean an important amount of suspended particulate matter in the form of turbid plumes. The surface plume is more particularly studied from coastal oceanographic surveys and NOAA/AVHRR satellite data collected during a French programme of coastal oceanography (PNOC-Atlantic). The AVHRR reflectances are atmospherically corrected according to an algorithm based on the clear water concept. The comparison with suspended sediment concentrations are realised by direct and indirect calibrations. The correlations obtained show that at a same concentration the reflectance varies, probably because of varying particle (floc) size and composition. The shape and the surface measured from low resolution (4 km × 4 km) and high resolution (1 km × 1 km) AVHRR data are then compared to the forcings introduced by the fluvial output, the tide, neap and spring tide and the wind variations. The latter have an important effect on the orientation of the distal part of the turbid plume.     

Bioaccumulation des métaux chez le mytilidae hydrothermal Bathymodiolus sp. de la ride médio-atlantique

The hydrothermal environment is characterised, among other things, by a high metal concentration, related to the convective sea-water circulation inside the oceanic crust and its interaction with basaltic rocks. The biological communities associated with the hydrothermal system can survive in this toxic environment owing to their ability to regulate their intracellular metal levels by excretion or accumulation of metal ions in non-toxic forms. Various detoxification processes have been previously studied within an hydrothermal organism: immobilisation and precipitation of the metal in lysosomal systems, or binding to specific and soluble ligands such as metallothioneins. The later are heat stable and characterised by a high content of cysteine. The quantification of Fe, Zn, Cu, Mn, Cd, Ag, Ba and Sr in the tissues of the vent mussel Bathymodiolus sp. indicates a high accumulation of these metals in two target organs, the gill and the digestive gland. This accumulation is in relation with high concentrations of metallothioneins, but the subcellular distribution of metals indicates a higher contribution of the insoluble compartment for detoxification processes. These results are compared with the literature data about metal bioaccumlation in Bathymodiolus thermophilus collected at the Galapagos Rift.     

Modélisation sur vingt ans (1976–1995) de la production phytoplanctonique en baie de Seine

This paper presents an ecological modelling of the Bay of Seine (Eastern Channel) over the last twenty years, chosen as a typical case of eutrophication in a river plume. In the physical sub-model, the Bay is divided into 42 boxes and water fluxes between them are calculated automatically using Ifremer's “Elise” software. A two-layer, vertical thermohaline model is then linked with the horizontal circulation scheme in order to take vertical stratification into account. The biological submodel deals with two chemical elements, nitrogen and silicon, and splits phytoplankton into diatoms and flagellates. Results from this ecological model point out the spatial concordance of highest phytoplanktonic concentrations with the river plume spreading in the bay. Contrary to diatoms, flagellate production appears to be mainly confined to the eastern bay, due to the vertical haline stratification in front of the river mouth. As far as the whole bay is concerned, the interannual fluctuations of diatom production are related to the level of spring insolation, whereas silicon inputs regulate diatom production in the river plume. The flagellate summer production in the plume is enhanced by high water temperature and high N/Si ratios, which appear during dry years with low discharge regimes. Finally, interannual increase of flagellate production could be related to gradual increase of nitrogen loadings, contrasting with silicon loadings, which remained stable for twenty years.     

Relations milieu-ressources: impact de la conchyliculture sur un environnement lagunaire méditerranéen (Thau)

Shellfish farming leaves its mark on the environment in which it has developed, and the men who depend upon it. These changes have altogether balanced the lagoon cycle and have caused disastrous episodic events. Increased water clarity caused by the uptake of particulate material by shellfish farming allows seagrass to grow in deeper areas of the lagoon (down to five metres). Shellfish farming nutrient transformations increase ecosystem productivity, even if the filtration pressure keeps phytoplankton biomass at a low level. Storage of phosphorus and nitrogen in animal tissue limits eutrophication in this ecosystem. Transfer of oysters from growout facilities increases animal and vegetal specific diversity. The presence of large amounts of shellfish allows for the development of a masive benthos, while organic enrichment from biodeposition changes the specific composition of soft-bottom benthos. In the deeper areas, (less than six metres), where summer thermoclines limit oxygen transfer from surface water, the organically enriched substrate induces oxygen depletion and ammonium and nitrogen sulfide accumulation in the water column. This ecosystem dysfunction kills benthic populations, and sometimes reaches pelagic populations and affects the shellfish farming economy.     

Sur l'existence d'une topographie antérécifale dans la région de Tuléar (côte sud ouest de Madagascar)

The outline of the Pleistocene coastal geomorphology in the Tuléar area (Madagascar) can be redrawn from the analysis of the setting and characteristics of present-day coral reefs. Initial outer reef tracts, which grew in Tatsimian times (according to Battistini's terminology), followed in their development the river pattern, since they settled upon the divides between valleys instead of growing uniformly on the outer shelf. These primary reef chains coalesced subsequently. Inner reefs and coral banks also followed, although not so closely, the river pattern.     

Etude de la variabilité des teneurs en Hg,Cu, Zn et Pb de trois espèces de mollusques de l'étang de Berre (France)

The bioaccumulation of Hg, Cu, Zn and Pb ions was studied in three mollusc species at three sites in the Berre lagoon (Marseille, France), which have been selected according to their hydrodynamic conditions. The degree of the contamination of these mollusc populations was calculated in order to determine the levels of the metal ions present. The uptake of metal ions was regularly monitored for two successive years; the causes of the high variability was observed throughout the two years for all the species studied are discussed.     

La dispersion des apports de la Gironde sur le plateau continental. Données in situ,satellitales et numériques

Coastal waters are generally a product of mixing between continental and oceanic originated water masses. Near river mouth areas, as in the Gironde estuary, oceanic waters are modified by the influence of freshwater discharge that introduces suspended and dissolved substances, including pollutants. Within PNOC (Programme National d'Oceanographie Côtière) our objective is to determine the extent of the influence of the Gironde estuary on to the Aquitanian continental shelf. Several cruises for collecting hydrological data have been carried out in this area. The data of every cruise were coupled with a NOAA-11 satellite passage. The recorded images have been analysed and were compared with the results given by a 3D mathematical model. The interaction among different dynamical factors (density, wind-driven and tidal circulation) controls the mixing and the transport of low salinity waters.     

Caractéristiques du feutrage algal des récifs coralliens de Polynésie française soumis à différentes intensités de bioérosion

This study deals with the colonisation of experimental Porites blocks by epilithic and endolithic algal community in two atoll lagoons and two high-island lagoons in French Polynesia, exposed to different environmental conditions during a 5-year period. A qualitative analysis of the community (cyanobacteria and micro-algae) was carried out, and the relationship between the epilithic and endolithic strata observed. HPLC analysis of chlorophyll pigments was used to quantitatively determine the plant biomass, which was then compared to that of the algal turf of neighbouring reefs. The results show that these blocks are colonised according to two factors: the reef structure (atoll or high-island) and the degree of human disturbance. The epilithic and endolithic population dynamics differ.The density of the epilithic algal community and the species diversity increase with the degree of eutrophication and the amount of borers (sea-urchins, parrotfish), while the proportion of endolithic species remains low (34 %–59 %). This is especially marked in the high-island lagoons, where leaching has caused enhanced nutrient levels. An extreme case is the station at Faaa, a highly polluted harbour, where increased sea-urchin levels (44 ind. m⁻²) meant that the blocks disappeared completely in less than five years due to bioerosion. In the oligotrophic waters of the atoll lagoons, the losses of CaCO₃ by bioerosion do not exceed 35 % per block, and the algal community is made up mainly of endolithic species (66 %–85 %). The algal biomass is lowest in the Takapoto atolls (10–11 μg cm⁻²) and maximum in Tikehau atoll (23 and 56 μg cm⁻²). The first microborers to settle in the ecological succession of the community are the cyanobacteria. These are gradually replaced by Ostreobium (Chlorophyceae borers), which become dominant in the blocks exposed to the lowest bioerosion rate. The richness and the density of the algal turf reflect the convergence of many ecological factors: it seems that the covering of epilithic algae or the richness of endolithic flora could be used as a global indicator of the quality and the health of a reef.     

Croissance de juvéniles de Nereis diversicolor nourris avec des détritus d’halophytes

The comparative effect of the uptake of fresh and degraded detritus of halophytic plants, harvested from salt marches of the Mont Saint-Michel Bay (France), on the growth of a juvenile population of the annelid polychaete N. diversicolor (L.) was studied under experimental conditions in summer 1993. Fresh and degraded detritus of Spartina anglica, Halimione portulacoides and Salicornia europeae, as well as the green algae Enteromorpha sp. were distributed separately to homogenous set of juveniles for a 75 d period. The highest growth rate was obtained for worms fed with Enteromorpha sp. Administration of fresh detritus of a given halophyte species to N. diversicolor juveniles always leads to a significantly higher growth rate than did degraded detritus. This is probably due to a great microbial biomass occurring on the fresh detritus, which is moreover, much better assimilated than the detritus they colonize.