| 新疆沙冬青叶片蛋白质的双向电泳技术研究 |
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| 引用本文： | 鲁春芳,尹林克. 新疆沙冬青叶片蛋白质的双向电泳技术研究[J]. 中国沙漠, 2011, 31(1): 96-100 |
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| 作者姓名： | 鲁春芳 尹林克 |
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| 作者单位： | （1.中国科学院 新疆生态与地理研究所 干旱区生物地理与生物资源重点实验室, 新疆 乌鲁木齐 830011; 2.中国科学院 研究生院, 北京 100049） |
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| 基金项目： | 新疆维吾尔自治区“十一五”重大科技专项(200731138-2); 新疆维吾尔自治区自然科学基金项目(200821160); 新疆生物资源基因工程重点实验室开放课题(XJDX0201-2005-09)资助 |
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| 摘 要： | 提取新疆沙冬青叶片中蛋白质受到脂类、酚类等次生代谢产物的干扰,针对这种现象,进行了组分提取优化方法和双向电泳技术的改进。实验分析表明,用乙醚淋洗完整叶片,50 mmol·L-1 Tris-HCl缓冲液,上样量240 μg（7 cm胶条）,考马斯亮蓝染色等方法可很大程度增加提取可溶性蛋白含量,结合丙酮-三氯乙酸的沉降,可较完整地提取蛋白质组。
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| 关 键 词： | 蛋白质组 提取 双向电泳 新疆沙冬青 |
| 收稿时间： | 2010-05-26 |
| 修稿时间： | 2010-09-11 |
Two-dimensional Electrophoresis of Proteins Extraction from Ammopiptanthus nanus(M.Pop.)Cheng f.Leaves |
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| LU Chun-fang,YIN Lin-ke. Two-dimensional Electrophoresis of Proteins Extraction from Ammopiptanthus nanus(M.Pop.)Cheng f.Leaves[J]. ournal of Desert Research, 2011, 31(1): 96-100 |
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| Authors: | LU Chun-fang YIN Lin-ke |
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| Affiliation: | (1.Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China; 2.Graduate University of Chinese Academy of Sciences, Beijing 100049, China) |
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| Abstract: | Proteins extraction from leaves of Ammopiptanthus nanus(M.Pop.) Cheng f.is disturbed by some secondary metabolites,such as lipoidal and phenolic substances.To overcome this problem,method of proteins extraction and two-dimensional electrophoresis protocol of Ammopiptanthus.nanus(M.Pop.)Cheng f.were optimized and ameliorated in this paper.Further,study of preparing method,quantitative loading of samples and staining means were conducted.The results show that washing whole leafs with aether,extracting soluble... |
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| Keywords: | proteome extraction two-dimensional electrophoresis Ammopiptanthus.nanus(M.Pop.)Cheng f. |
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