| 苯并（a）芘诱导下岩虫CYP基因的表达特征 |
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| 引用本文： | 李婉娟,赵欢,Ba Fuyang,李绍娟,孙秀鹏,杨大佐,周一兵. 苯并（a）芘诱导下岩虫CYP基因的表达特征[J]. 海洋学报(英文版), 2019, 38(6): 46-53. DOI: 10.1007/s13131-019-1362-1 |
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| 作者姓名： | 李婉娟 赵欢 Ba Fuyang 李绍娟 孙秀鹏 杨大佐 周一兵 |
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| 作者单位： | 辽宁省海洋资源恢复与生境修复重点实验室, 大连海洋大学, 大连, 116023,辽宁省海洋资源恢复与生境修复重点实验室, 大连海洋大学, 大连, 116023,Marine and Fisheries Technology Center of Pan Shan Country, Panjin 124000, China,辽宁省海洋资源恢复与生境修复重点实验室, 大连海洋大学, 大连, 116023,辽宁省海洋资源恢复与生境修复重点实验室, 大连海洋大学, 大连, 116023,辽宁省海洋资源恢复与生境修复重点实验室, 大连海洋大学, 大连, 116023,辽宁省海洋资源恢复与生境修复重点实验室, 大连海洋大学, 大连, 116023 |
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| 基金项目： | The Dalian Science Fund for Distinguished Young Scholars under contract No. 2016RJ06; the Natural Science Foundation of Liaoning Province under contract No. 201602107; the Superior College Science Technology Research Project of Liaoning Province under contract No. ZL201701; the National Natural Science Foundation of China under contract No. 41306138. |
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| 摘 要： | Rapid amplification of cDNA ends(RACE) and real-time polymerase chain reaction(RT-PCR) were carried out to analyze the CYP4 gene expression in polychaete Marphysa sanguinea exposed to benzo[a]pyrene(BaP) in this study. The full length of MsCYP4 cDNA was 2 470 bp, and it encoded 512 amino acids. The deduced amino acid sequence showed 47% identity with CYP4 F from frog Xenopus tropicalis and shared high homology with other known CYP4 sequences. To analyse the role of CYP4 in protecting M. sanguinea from BaP exposure, three BaP groups were established: 0.5, 5 and 50 μg/L. Polychaetes were sampled after 3, 7 and 12 d. At 0.5 μg/L, the effect of BaP on MsCYP4 gene expression increased with time prolonged. MsCYP4 gene expression curve showed Ushaped trend with time in 5 and 50 μg/L BaP groups. Therefore, MsCYP4 gene may play an important role in maintaining the balance of cellular metabolism and protecting M. sanguinea from BaP toxicity.
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| 关 键 词： | 岩虫 CYP4 苯并(a)芘 毒性效应 |
| 收稿时间： | 2017-06-06 |
mRNA expression of CYP4 in marine polychaete Marphysa sanguinea in response to benzo[a]pyrene |
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| Li Wanjuan,Zhao Huan,Ba Fuyang,Li Shaojuan,Sun Xiupeng,Yang Dazuo and Zhou Yibing. mRNA expression of CYP4 in marine polychaete Marphysa sanguinea in response to benzo[a]pyrene[J]. Acta Oceanologica Sinica, 2019, 38(6): 46-53. DOI: 10.1007/s13131-019-1362-1 |
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| Authors: | Li Wanjuan Zhao Huan Ba Fuyang Li Shaojuan Sun Xiupeng Yang Dazuo Zhou Yibing |
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| Affiliation: | 1.Key Laboratory of Marine Bioresource Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Dalian 116023, China2.Marine and Fisheries Technology Center of Pan Shan Country, Panjin 124000, China |
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| Abstract: | Rapid amplification of cDNA ends (RACE) and real-time polymerase chain reaction (RT-PCR) were carried out to analyze the CYP4 gene expression in polychaete Marphysa sanguinea exposed to benzo[a]pyrene (BaP) in this study. The full length of MsCYP4 cDNA was 2 470 bp, and it encoded 512 amino acids. The deduced amino acid sequence showed 47% identity with CYP4F from frog Xenopus tropicalis and shared high homology with other known CYP4 sequences. To analyse the role of CYP4 in protecting M. sanguinea from BaP exposure, three BaP groups were established: 0.5, 5 and 50 μg/L. Polychaetes were sampled after 3, 7 and 12 d. At 0.5 μg/L, the effect of BaP on MsCYP4 gene expression increased with time prolonged. MsCYP4 gene expression curve showed U-shaped trend with time in 5 and 50 μg/L BaP groups. Therefore, MsCYP4 gene may play an important role in maintaining the balance of cellular metabolism and protecting M. sanguinea from BaP toxicity. |
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| Keywords: | Marphysa sanguinea CYP4 benzo[a]pyrene toxicity effect |
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