Culture medium optimization for pigment production with RSM method |
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| Authors: | WU Hongqing TIAN Li ZHANG Jiuming SUN Zhaobin HUANG Leping |
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| Affiliation: | First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China;Department of Oceanic Science and Technology, Hebei University of Technology, Tianjin 300130, China;First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China;Qingdao University of Science and Technology, Qingdao 266042, China;First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China;Southwest Forestry College, Kunming 650224, China;Xinjiang Academy of Agricultural Science, Wulumuqi 83000, China |
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| Abstract: | Response surface methodology was used to optimize a medium for a red-pigmented marine bacterium S-9801 strain (Flavobacterium sp.). In the first optimization step the influence of yeast extract, peptone, glucose and sodium chloride on pigment production was evaluated using a fractional factorial design. Pigment production was positively influenced by glucose and sodium chloride while other components had no significant effect. In the second step the path of steepest ascent was used to approach the optimal region of the medium composition. In the third step the optimal concentration of glucose and sodium chloride was determined by a central composite design and response analysis. The optimized medium allowed pigment production (A535~650) to be increased from 0.137 to 0.559, being 320% higher than the original medium. |
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| Keywords: | response surface methodology red pigment optimization |
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