| 糖原含量与糖原磷酸化酶基因(GPH)和己糖激酶基因(HK)的表达在福建牡蛎生殖周期中的相关性分析 |
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| 引用本文： | 曾臻,倪健斌,柯才焕. 糖原含量与糖原磷酸化酶基因(GPH)和己糖激酶基因(HK)的表达在福建牡蛎生殖周期中的相关性分析[J]. 海洋学报(英文版), 2015, 34(6): 66-76. DOI: 10.1007/s13131-015-0639-2 |
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| 作者姓名： | 曾臻 倪健斌 柯才焕 |
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| 作者单位： | 厦门大学近海海洋环境科学国家重点实验室, 厦门 361005;厦门大学海洋与地球学院, 厦门 361005,厦门大学近海海洋环境科学国家重点实验室, 厦门 361005;厦门大学海洋与地球学院, 厦门 361005;国家海洋局海洋减灾中心, 北京 100093,厦门大学近海海洋环境科学国家重点实验室, 厦门 361005;厦门大学海洋与地球学院, 厦门 361005 |
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| 基金项目： | The National Basic Research Program (973 program) of China under contract No. 2010CB126403; the Program for Changjiang Scholars and Innovative Research Team of Xiamen University under contract No. IRT0941; the Earmarked Fund for Modern Agro-industry Technology Research System under contract No. nycytx-47; the Programme of Introducing Talents of Discipline to Universities under contract No. B07034. |
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| 摘 要： | Glycogen, a polymer of glucose, is an important means of storing energy. It is degraded by glycogen phosphorylase (GPH) and hexokinase (HK), glycogen phosphorylase, and hexokinase cDNAs (Ca-GPH andCa-H...
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| 关 键 词： | 福建牡蛎 糖原磷酸化酶基因 己糖激酶基因 糖原代谢 生殖周期 |
| 收稿时间： | 2014-02-20 |
| 修稿时间： | 2014-07-04 |
Glycogen content relative to expression of glycogen phosphorylase (GPH) and hexokinase (HK) during the reproductive cycle in the Fujian Oyster, Crassostrea angulata |
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| ZENG Zhen,NI Jianbin and KE Caihuan. Glycogen content relative to expression of glycogen phosphorylase (GPH) and hexokinase (HK) during the reproductive cycle in the Fujian Oyster, Crassostrea angulata[J]. Acta Oceanologica Sinica, 2015, 34(6): 66-76. DOI: 10.1007/s13131-015-0639-2 |
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| Authors: | ZENG Zhen NI Jianbin KE Caihuan |
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| Affiliation: | 1.State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China;College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, China2.State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China;College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, China;National Marine Hazard Mitigation Service, Beijing 100194, China |
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| Abstract: | Glycogen, a polymer of glucose, is an important means of storing energy. It is degraded by glycogen phosphorylase (GPH) and hexokinase (HK), glycogen phosphorylase, and hexokinase cDNAs (Ca-GPH and Ca-HK, respectively), which encode the primary enzymes involved in glycogen use, cloned and characterized and used to investigate the regulation of glycogen metabolism at the mRNA level in Crassostrea angulata. Their expression profiles were examined in different tissues and during different reproductive stages. Full-length cDNA of GPH was 3 078 bp in length with a 2 607 bp open reading frame (ORF) predicted to encode a protein of 868 amino acids (aa). The full-length HK cDNA was 3 088 bp long, with an ORF of 1 433 bp, predicted to encode a protein of 505 aa. Expression levels of both genes were found to be significantly higher in the gonads and adductor muscle than in the mantle, gill, and visceral mass. They were especially high in the adductor muscle, which suggested that these oysters can use glycogen to produce a readily available supply of glucose to support adductor muscle activity. The regulation of both genes was also found to be correlated with glycogen content via qRT-PCR and in situ hybridization and was dependent upon the stage of the reproductive cycle (initiation, maturation, ripeness). In this way, it appears that the expression of Ca-GPH and Ca-HK is driven by the reproductive cycle of the oyster, reflecting the central role played by glycogen in energy use and gametogenic development in C. angulata. It is here suggested that Ca-GPH and Ca-HK can be used as useful molecular markers for identifying the stages of glycogen metabolism and reproduction in C. angulata. |
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| Keywords: | Crassostrea angulate GPH HK glycogen metabolism reproduction |
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