西太平洋暖池核心区Ontong Java 海台古菌多样性研究
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Archaeal diversity in the Ontong Java plateau from center of the Western Pacific Warm Pool
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    摘要:

    为了研究西太平洋暖池核心区表层古菌群落结构及多样性,应用16S rDNA文库技术对KX08-973-22站位(取名为CWP)水体样构建基因文库,得到了561个克隆,经分析处理后得到50个OUT (Operational Taxonimic Units)。通过对16SrRNA基因序列的同源性比较, 构建系统发育树分析及统计学分析,得出的结果:本次研究中的古菌类群都属于广古菌(Euryarchaeota)和泉古(Crenarchaeota)。广古菌以marine group II(MG II)(49.0%)为主,marine group III(MG III)占一小部分(5.7%)。泉古菌以Marine Group I(MG I)为主(45.1%),Miscellaneous Crenarchaeotic Group(MCG)占极少数(0.2%)。得出结论是本次研究的西太平洋暖池核心区表层古菌多样性不高,群落结构在小尺度上存在垂直变化。

    Abstract:

    To study the archaeal community structure and diversity, clone libraries were constructed from water samples of the KX08-973-22 site(named CWP)in the core area of west Pacific Warm Pool by using the 16S rDNA library technology. Finally, 561 clones were obtained and 50 operational taxonomic units (OTUs) were identified after analysis. By homologous comparing for the 16S rRNA gene sequences, constructing phylogenetic tree and statistical analysis, we obtained the following results: all the archaeal phylotypes in this study were affiliated with Euryarchaeota and Crenarchaeota, respectively. For the Euryarchaeota, Marine Group II (MGII, 49.0%) were the most predominant phylotypes and marine group III (MGIII) only accounted for 5.7% of the libraries. For the Crenarchaeota, marine group I(MGI) dominated the clone libraries, and Miscellaneous Crenarchaeotic Group (MCG, 0.2%) constituted a small proportion of the libraries. Our study suggested that archaeal phylotypes from the core surface area of the west Pacific Warm Pool were not high diversity and community structure showed stratification within small vertical distances

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春梅,萨仁高娃,李铁刚,莫内,李保华,于心科.2011.西太平洋暖池核心区Ontong Java 海台古菌多样性研究[J].地质学报,85(8):1368-1376.
chunmei, sarengaowa, litiegang, monei, libaohua, yuxinke.2011. Archaeal diversity in the Ontong Java plateau from center of the Western Pacific Warm Pool[J]. Acta Geologica Sinica,85(8):1368-1376.

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  • 收稿日期:2010-12-03
  • 最后修改日期:2011-04-15
  • 录用日期:2011-04-19
  • 在线发布日期: 2011-08-09