Cloning, expressing, and hemolysis of tdh, trh and tlh genes of Vibrio parahaemolyticus |
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| Authors: | Yonggang Zhao Xiaoqian Tang Wenbin Zhan |
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| Institution: | 1. Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, P. R. China;China Animal Health and Epidemiology Center, Qingdao 266032, P. R. China
2. Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, P. R. China |
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| Abstract: | Vibrio parahaemolyticus (VP) is one of the pathogenic vibrios endangering net-cage cultured Pseudosciaena crocea,Fennerpenaeus chinensis, and shellfish in coastal areas of China. Several types of hemolysins produced by Vp have been characterized as major virulence factors.They are thermostable direct hemolysin (TDH),TDH-related hemolysin (TRH) and thermolabile hemolysin (TLH). In this study, we cloned tdh, trh, and tlh genes from the genome DNA of VP by polymerase chain reaction (PCR).We ligated the three g... |
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| Keywords: | Vibrio parahaemolyticus hemolysin clone expression hemolytic activity |
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