| Bioflocculant Produced by Klebsiella sp. MYC and Its Application in the li＇eatment of Oil-field Produced Water |
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| 作者姓名： | YUE Lixi MA Chunling CHI Zhenming |
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| 作者单位： | UNESCO Chinese Center of Marine Biotechnology , （~an University of China, Qingdao 266003, P. R. China |
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| 基金项目： | Acknowledgements All the authors cordially them Shengli 0il Company in Dongying for its financial support. |
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| 摘 要： | Seventy-nine strains of bioflocculant-producing bacteria were isolated from 3 activated sludge samples. Among them, strain MYC was found to have the highest and stable flocculating rate for both kaolin clay suspension and oil-field produced water. The bacterial strain was identified as Klebsiella sp. MYC according to its morphological and biochemical characteristics and 16SrDNA sequence. The optimal medium for bioflocculant production by this bacterial strain was composed of cane sugar 20 g L^-1, KH2PO4 2 g L^-1, K2HPO4 5 g L^- 1, （ NH4）2SO4 0.2 g L^-1, urea 0.5 g L^- 1 and yeast extract 0.5 g L^- 1, the initial pH being 5.5. When the suspension of kaolin clay was treated with0.5% of Klebsiella sp. MYC culture broth, the flocculating rate reached more than 90.0% in the presence of 500mg L^-1 CaCI2, while the flocculating rate for oil-field produced water was near 80.0% in a pH range of 7.0 - 9.0 with the separation of oil and suspended particles from the oil-field produced water under similar conditions. The environment-friendly nature of the bioflocculant and high flocculating rate of the strain make the bioflocculant produced by Klebsiella sp. MYC an attractive bioflocculant in oil-field produced water treatment.
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| 关 键 词： | 生物凝聚剂 油田废水 废水处理 活性污泥 细菌分离 克雷伯氏菌属 |
| 收稿时间： | 2006-03-14 |
| 修稿时间： | 2006-09-07 |
Bioflocculant produced by Klebsiella sp. MYC and its application in the treatment of oil-field produced water |
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| YUE Lixi MA Chunling CHI Zhenming.Bioflocculant produced by Klebsiella sp. MYC and its application in the treatment of oil-field produced water[J].Journal of Ocean University of China,2006,5(4):333-338. |
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| Authors: | Lixi Yue Chunling Ma Zhenming Chi |
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| Institution: | (1) UNESCO Chinese Center of Marine Biotechnology, Ocean University of China, 266003 Qingdao, P. R. China |
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| Abstract: | Seventy-nine strains of bioflocculant-producing bacteria were isolated from 3 activated sludge samples. Among them, strain
MYC was found to have the highest and stable flocculating rate for both kaolin clay suspension and oil-field produced water.
The bacterial strain was identified as Klebsiella sp. MYC according to its morphological and biochemical characteristics and 16SrDNA sequence. The optimal medium for bioflocculant
production by this bacterial strain was composed of cane sugar 20gL−1 KH2PO4 2g L−1, K2HPO45gL−1, (NH4)2SO4 0.2gL−1, urea 0.5 gL−1 and yeast extract 0.5 gL−1, the initial pH being 5.5. When the suspension of kaolin clay was treated with 0.5% of Klebsiella sp. MYC culture broth, the flocculating rate reached more than 90.0% in the presence of 500mgL1 CaCl2, while the flocculating rate for oil-field produced water was near 80.0% in a pH range of 7.0–9.0 with the separation of
oil and suspended particles from the oil-field produced water under similar conditions. The environment-friendly nature of
the bioflocculant and high flocculating rate of the strain make the bioflocculant produced by Klebsiella sp. MYC an attractive bioflocculant in oil-field produced water treatment. |
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| Keywords: | bioflocculant Klebsiella sp MYC oil-field produced water |
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