| 菲律宾蛤仔糖胺聚糖的纯化及单糖组成的研究 |
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| 引用本文： | 王瑞芳,吴红棉,范秀萍.菲律宾蛤仔糖胺聚糖的纯化及单糖组成的研究[J].广东海洋大学学报,2008,28(6). |
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| 作者姓名： | 王瑞芳 吴红棉 范秀萍 |
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| 作者单位： | 广东海洋大学食品科技学院,广东,湛江,524025 |
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| 基金项目： | 广东海洋大学团队项目
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| 摘 要： | 菲律宾蛤仔中提取的糖胺聚糖(GAGs)粗品,经DEAE-52-离子交换柱层析和Sephadex G-100凝胶柱纯化后,采用Sephadex G-100凝胶柱层析法测定该糖胺聚糖纯品F-1-1的相对分子质量,并以薄板层析(TLC)法对F-1-1的酸水解液进行单糖组成分析。结果表明:两级纯化得到均一的糖胺聚糖,其相对分子质量为4.4×104,单糖组分主要为氨基半乳糖、半乳糖、葡萄糖;对多糖进行两级纯化效果较好,纯品可进行结构分析;TLC法可快速分析水解多糖的单糖组成。
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| 关 键 词： | 糖胺聚糖 相对分子质量 薄层色谱 单糖组成 菲律宾蛤仔 |
Purification and Monosaccharide Composition Analysis of Glycosaminoglycans of Ruditapes philippinarum |
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| WANG Rui-fang,WU Hong-mian,FAN Xiu-ping.Purification and Monosaccharide Composition Analysis of Glycosaminoglycans of Ruditapes philippinarum[J].Journal of Zhanjiang Ocean University,2008,28(6). |
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| Authors: | WANG Rui-fang WU Hong-mian FAN Xiu-ping |
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| Institution: | WANG Rui-fang,WU Hong-mian,FAN Xiu-ping(College of Food Science & Technology,Guangdong Ocean University,Zhanjiang 524025,China) |
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| Abstract: | Crude Glycosaminoglycans(GAGs) was extracted from Ruditapes philippinarum.The product was purified by DEAE-52 cellulose and SephadexG-100 column chromatography respectively,and then the pure Glycosaminoglycans was obtained.The molecular weight of F-1-1 was 4.4×104 calculated by Sephadex G-100 gel chromatography analysis.After acid hydrolysis,Glycosaminoglycans was analyzed by thin-layer chromatography(TLC),the monosaccharide composition of F-1-1 was determinated to be D-Galactosamine、galactose and glucose. |
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| Keywords: | Glycosaminoglycans molecular weight thin-layer chromatography monosaccharide composition Ruditapes philippinarum |
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