| 不同方法提取马氏珠母贝外套膜胶原蛋白理化性质比较 |
| |
| 引用本文： | 林海生,王雯,秦小明,曹文红,章超桦,高加龙,郑惠娜,陈忆宾,伍彬.不同方法提取马氏珠母贝外套膜胶原蛋白理化性质比较[J].广东海洋大学学报,2022(1). |
| |
| 作者姓名： | 林海生 王雯 秦小明 曹文红 章超桦 高加龙 郑惠娜 陈忆宾 伍彬 |
| |
| 作者单位： | ;1.广东海洋大学食品科技学院;2.广东省水产品加工与安全重点实验室//广东普通高等学校水产品深加工重点实验室//国家贝类加工技术研发分中心(湛江)//南海生物资源开发与利用协同创新中心;3.大连工业大学海洋食品精深加工关键技术省部共建协同创新中心;4.海南盛美诺生物技术有限公司 |
| |
| 基金项目： | 广东海洋大学博士启动项目(R17082);2021年度省科技专项资金(“大专项+任务清单”)项目(2021A201-11);财政部和农业农村部:国家现代农业产业技术体系资助(CARS-49);广东省现代农业产业技术体系创新团队项目(2021 KJ146);广东海洋大学贝类高值化加工利用创新团队(CXTD2021004);广东海洋大学创新强校专项项目(Q18292);湛江市科技计划项目(2014C01019)。 |
| |
| 摘 要： | 【目的】系统分析不同方法提取的马氏珠母贝(Pinctada martensii)外套膜胶原蛋白的理化性质及生物学功能。【方法】分别采用酸-酶法和热水法从外套膜中提取得到两种胶原蛋白(A-PSC(Pm)和HSC(Pm)),对其氨基酸组成、微观结构及热变性温度等理化特性进行比较分析。【结果】HSC(Pm)提取率高于A-PSC(Pm);HSC(Pm)中,Gly、Glu与Arg相对含量较高,分别为20.16%、17.57%和10.07%;而A-PSC(Pm)中,Glu、Asp与Arg相对含量为19.67%、11.63%和10.07%;紫外光谱和红外光谱分别显示,两种胶原蛋白均在220 nm左右有强吸收峰,且具有典型的胶原蛋白特征带(酰胺A、B、Ⅰ、Ⅱ和Ⅲ带);扫描电镜结果显示,A-PSC(Pm)呈致密多孔网状结构,HSC(Pm)则基本为薄片状;DSC分析结果表明,HSC(Pm)具有更好的热稳定性。【结论】酸-酶法及热水法提取的胶原蛋白均具有类V型胶原蛋白特性,其结构特征及其理化性质均存在差异,可根据需求选择特定的胶原蛋白提取方法用以开发不同类型的产品。
|
| 关 键 词： | 马氏珠母贝 外套膜 胶原蛋白 理化性质 提取方法 |
Characterization of Collagens from Mantle of Pinctada martensii by Different Extraction Methods |
| |
| LIN Hai-sheng,WANG Wen,QIN Xiao-ming,CAO Wen-hong,ZHANG Chao-hua,GAO Jia-long,ZHENG Hui-na,CHEN Yi-bin,WU Bin.Characterization of Collagens from Mantle of Pinctada martensii by Different Extraction Methods[J].Journal of Zhanjiang Ocean University,2022(1). |
| |
| Authors: | LIN Hai-sheng WANG Wen QIN Xiao-ming CAO Wen-hong ZHANG Chao-hua GAO Jia-long ZHENG Hui-na CHEN Yi-bin WU Bin |
| |
| Institution: | (College of Food Science and Technology,Guangdong Ocean University,Zhanjiang 524088,China;Guangdong Key Laboratory of Aquatic Products Processing and Safety/Key Laboratory of Aquatic Products Deep Processing of Guangdong Universities/National R&d Center of Shellfish Processing Technology(Zhanjiang)/South China Sea Biological Resources Development and Utilization Collaborative Innovation Center,Zhanjiang 524088,China;Dalian Polytechnic University/Collaborative Innovation Center of Seafood Deep Processing,Dalian 116034,China;Hainan Semnl Biotechnology Co.,LTD,Chengmai 571925,China) |
| |
| Abstract: | 【Objective】Collagen were extracted from the mantle of Pinctada martensii by different methods and their physical and chemical properties were analyzed,with aims to provide theoretical basis for the subsequent development of collagen products.【Methods】Collagens were extracted by acid-pepsic extraction and hot-water extraction,named acid-pepsin soluble collagen(A-PSC(Pm))and hot water soluble collagen(HSC(Pm)).The amino acid composition,microstructure,secondary structure and thermal denature-temperature of both collagens were comparatively analyzed.【Results】The collagen extraction rate by hot water method was higher than that by acid-pepsic method.The contents of Gly,Glu and Arg in HSC(Pm)were the highest,which were 20.16%,17.57%and 10.07%,respectively.The contents of Glu,Asp and Arg were about 19.67%,11.63%and 10.07%respectively in A-PSC(Pm).UV and IR spectrums showed that HSC(Pm)and A-PSC(Pm)had strong absorption peak at 220 nm,and had typical characteristic bands of collagen(amide A,B,Ⅰ,ⅡandⅢ).The results of scanning electron microscopy(SEM)showed that A-PSC(Pm)was presented as dense porous network structure,while HSC(Pm)was basically in laminar.DSC analysis results show that HSC(Pm)has better thermal stability.【Conclusion】These results indicated that both HSC(Pm)and A-PSC(Pm)had V-like collagen properties,and there were differences in both structural characteristics and physicochemical properties.In conclusion,different collagen products could be developed according to pearl oyster collagen extraction methods. |
| |
| Keywords: | Pinctada martensii mantle collagen physicochemical properties extraction method |
| 本文献已被 维普 等数据库收录！ |
|
