| Development of a SCAR marker for male gametophyte of Gracilariopsis lemaneiformis based on AFLP technique |
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| 作者姓名： | 周伟 丁弘叶 隋正红 王钟霞 王津果 |
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| 基金项目： | Supported by the ＂Twelfth Five-Year-Plan＂ of National Science and Technology for the Rural Development in China （No. 2012AA10A411） and the Public Welfare Project of the Ministry of Agriculture of China （No. 200903030） |
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| 摘 要： | The red alga Gracilariopsis lemaneiformis （Bory） is an economically valuable macroalgae. As a means to identify the sex of immature Gracilariopsis lemaneiformis, the amplified fragment length polymorphism （AFLP） technique was used to search for possible sex- or phase-related markers in male gametophytes, female gametophytes, and tetrasporophytes, respectively. Seven AFLP selective amplification primers were used in this study. The primer combination E-TG/M-CCA detected a specific band linked to male gametophytes. The DNA fragment was recovered and a 402-bp fragment was sequenced. However, no DNA sequence match was found in public databases. Sequence characterized amplified region （SCAR） primers were designed from the sequence to test the repeatability of the relationship to the sex, using 69 male gametophytes, 139 female gametophytes, and 47 tetrasporophytes. The test results demonstrate a good linkage and repeatability of the SCAR marker to sex. The SCAR primers developed in this study could reduce the time required for sex identification of Gracilariopsis lemaneiformis by four to six months. This can reduce both the time investment and number of specimens required in breeding experiments.
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| 关 键 词： | AFLP技术 SCAR标记 雄配子体 龙须菜 片段长度多态性 DNA片段 发育 序列匹配 |
Development of a SCAR marker for male gametophyte of Gracilariopsis lemaneiformis based on AFLP technique |
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| Wei Zhou,Hongye Ding,Zhenghong Sui,Zhongxia Wang,Jinguo Wang.Development of a SCAR marker for male gametophyte of Gracilariopsis lemaneiformis based on AFLP technique[J].Chinese Journal of Oceanology and Limnology,2014,32(3):522-526. |
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| Authors: | Wei Zhou Hongye Ding Zhenghong Sui Zhongxia Wang Jinguo Wang |
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| Institution: | 1. Key Laboratory of Marine Genetics and Breeding of Ministry of Education, Ocean University of China, Qingdao, 266003, China
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| Abstract: | The red alga Gracilariopsis lemaneiformis (Bory) is an economically valuable macroalgae. As a means to identify the sex of immature Gracilariopsis lemaneiformis, the amplified fragment length polymorphism (AFLP) technique was used to search for possible sex- or phase-related markers in male gametophytes, female gametophytes, and tetrasporophytes, respectively. Seven AFLP selective amplification primers were used in this study. The primer combination E-TG/M-CCA detected a specific band linked to male gametophytes. The DNA fragment was recovered and a 402-bp fragment was sequenced. However, no DNA sequence match was found in public databases. Sequence characterized amplified region (SCAR) primers were designed from the sequence to test the repeatability of the relationship to the sex, using 69 male gametophytes, 139 female gametophytes, and 47 tetrasporophytes. The test results demonstrate a good linkage and repeatability of the SCAR marker to sex. The SCAR primers developed in this study could reduce the time required for sex identification of Gracilariopsis lemaneiformis by four to six months. This can reduce both the time investment and number of specimens required in breeding experiments. |
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| Keywords: | Gracilariopsis lemaneiformis amplified fragment length polymorphism （AFLP） male gametophyte sequence characterized amplified region （SCAR） marker |
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