| A new identification method for five species of oysters in genus Crassostrea from China based on high-resolution melting analysis |
| |
| 作者姓名： | 王家丰 许飞 李莉 张国范 |
| |
| 基金项目： | Supported by the National Basic Research Program of China （973 Program） （No. 2010CB126402）, the National Natural Science Fotmdation of China （Nos. 40730845, 41206149）, the Shandong Provincial Natural Science Foundation （No. ZR2010DQ024）, the National High Technology Research and Development Program of China （863 Program） （No. 2012AA10A405）, the Earmarked Fund for Modem Ago-Industry Technology Research System （No. CARS-48）, the Taishan Scholar Program of Shandong Province, and the Taishan Scholar Climbing Program of Shandong Province |
| |
| 摘 要： | The high phenotypic plasticity in the shell of oysters presents a challenge during taxonomic and phylogenetie studies of these economically important bivalves. However, because DNA can exhibit marked differences among morphologically similar species, DNA barcoding offers a potential means for oyster identification. We analyzed the complete sequences of the cytochrome oxidase subunit I （COI） of five common Crassostrea species in China （including Hong Kong oyster C. hongkongensis, Jinjiang oyster C. ariakensis, Portuguese oyster C. angulata, Kumamoto oyster C. sikamea, and Pacific oyster C. gigas） and screened for distinct fragments. Using these distinct fragments on a high-resolution melting analysis platform, we developed an identification method that does not rely on species-specific PCR or fragment length polymorphism and is efficient, reliable, and easy to visualize. Using a single pair of primers （Oyster- COI-1）, we were able to successfully distinguish among the five oyster species. This new method provides a simple and powerful tool for the identification of oyster species.
|
| 关 键 词： | 识别方法 牡蛎壳 高分辨率 熔解 中国 物种特异性 细胞色素氧化酶 片段长度多态性 |
A new identification method for five species of oysters in genus Crassostrea from China based on high-resolution melting analysis |
| |
| Jiafeng Wang,Fei Xu,Li Li,Guofan Zhang.A new identification method for five species of oysters in genus Crassostrea from China based on high-resolution melting analysis[J].Chinese Journal of Oceanology and Limnology,2014,32(2):419-425. |
| |
| Authors: | Jiafeng Wang Fei Xu Li Li Guofan Zhang |
| |
| Institution: | 1. National & Local Joint Engineering Laboratory of Ecological Mariculture, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China
2. University of Chinese Academy of Sciences, Beijing, 100049, China
|
| |
| Abstract: | The high phenotypic plasticity in the shell of oysters presents a challenge during taxonomic and phylogenetic studies of these economically important bivalves. However, because DNA can exhibit marked differences among morphologically similar species, DNA barcoding offers a potential means for oyster identification. We analyzed the complete sequences of the cytochrome oxidase subunit I (COI) of five common Crassostrea species in China (including Hong Kong oyster C. hongkongensis, Jinjiang oyster C. ariakensis, Portuguese oyster C. angulata, Kumamoto oyster C. sikamea, and Pacific oyster C. gigas) and screened for distinct fragments. Using these distinct fragments on a high-resolution melting analysis platform, we developed an identification method that does not rely on species-specific PCR or fragment length polymorphism and is efficient, reliable, and easy to visualize. Using a single pair of primers (Oyster-COI-1), we were able to successfully distinguish among the five oyster species. This new method provides a simple and powerful tool for the identification of oyster species. |
| |
| Keywords: | identification cytochrome oxidase subunit I （COl） Crassostrea oyster high-resolution melting （HRM） |
| 本文献已被 CNKI 维普 SpringerLink 等数据库收录！ |
|
