| Polyploidy induction by hydrostatic pressure shock and embryo development of sea cucumber Apostichopus japonicus |
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| 作者姓名： | 丁君 常亚青 王子臣 宋坚 |
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| 作者单位： | [1]School of Envtronmental ＆ Biological Science ＆ Technology, Dalian University Technology, Dalian 116024, China [2]Key Laboratory of Mariculture and Biotechnology, Ministry of Agriculture, Dalian Fisheries University, Dalian 116023 China |
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| 基金项目： | 国家高技术研究发展计划(863计划);大连自然科学基金 |
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| 摘 要： | The manipulation of the chromosome set for commercially valuable marine animals is important for enhancing aquacultural production. In this study, triploid and tetraploid sea cucumber Apostichopus japonicus were induced by hydrostatic pressure shock, and the conditions of appropriate induction were tested with different starting times, and hydrostatic pressure intensities and durations. The highest rate of triploid induction reached 20% and that of tetraploid was 60%. In consideration of the survival rate and hatch rate, the appropriate treatment for triploid was 55 Mpa of hydrostatic pressure for 5 min at 55 min after fertilization (a.f.), while for tetraploid it was 60 Mpa for 5 min at 61 min a.f. The triploid of the sea cucumber could survive through the pelagic larval stage and attachment stage, and develop like the control group of the experiment. The tetraploid, however, could not survive the attachment stage.
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| 关 键 词： | 海参 多倍体 诱发因素 流体静力学压力冲击 胚胎发育 |
| 收稿时间： | 2005-06-20 |
| 修稿时间： | 2006-08-19 |
Polyploidy induction by hydrostatic pressure shock and embryo development of sea cucumber <Emphasis Type="Italic">Apostichopus japonicus</Emphasis> |
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| Ding?Jun,Chang?Yaqing,Wang?Zichen,Song?Jian.Polyploidy induction by hydrostatic pressure shock and embryo development of sea cucumber Apostichopus japonicus[J].Chinese Journal of Oceanology and Limnology,2007,25(2):184-190. |
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| Authors: | Ding Jun Chang Yaqing Wang Zichen Song Jian |
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| Institution: | (1) School of Environmental & Biological Science & Technology, Dalian University Technology, Dalian, 116024, China;(2) Key Laboratory of Mariculture and Biotechnology, Ministry of Agriculture, Dalian Fisheries University, Dalian, 116023, China |
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| Abstract: | The manipulation of the chromosome set for commercially valuable marine animals is important for enhancing aquacultural production. In this study, triploid and tetraploid sea cucumber Apostichopus japonicus were induced by hydrostatic pressure shock, and the conditions of appropriate induction were tested with different starting times, and hydrostatic pressure intensities and durations. The highest rate of triploid induction reached 20% and that of tetraploid was 60%. In consideration of the survival rate and hatch rate, the appropriate treatment for triploid was 55 Mpa of hydrostatic pressure for 5 min at 55 min after fertilization (a.f.), while for tetraploid it was 60 Mpa for 5 min at 61 min a.f. The triploid of the sea cucumber could survive through the pelagic larval stage and attachment stage, and develop like the control group of the experiment. The tetraploid, however, could not survive the attachment stage. |
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| Keywords: | triploid tetraploid hydrostatic pressure shock sea cucumber Apostichopus japonicus |
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