Gene cloning and prokaryotic expression of recombinant flagellin A from Vibrio parahaemolyticus |
| |
| Authors: | Ye Yuan Xiuli Wang Sheping Guo Yang Liu Hui Ge Xuemei Qiu |
| |
| Institution: | (1) Joint Sino-US Food Safety Research Center & Bor Luh Food Safety Center, School of Agriculture & Biology, Shanghai Jiao Tong University, 800 Dongchuan Rd., Shanghai, 200240, China;(2) National Engineering Center for Biochip at Shanghai, 151 Libing Rd., Shanghai, 201203, China;(3) Shanghai Entry–Exit Inspection and Quarantine Bureau, 1208 Minsheng Rd., Shanghai, 200135, China;(4) Department of Food Science and Technology, Shanghai Jiao Tong University, 800 Dongchuan Rd., Shanghai, 200240, China; |
| |
| Abstract: | The Gram-negative Vibrio parahaemolyticus is a common pathogen in humans and marine animals. Bacteria flagellins play an important role during infection and induction
of the host immune response. Thus, flagellin proteins are an ideal target for vaccines. We amplified the complete flagellin
subunit gene (flaA) from V. parahaemolyticus ATCC 17802. We then cloned and expressed the gene into Escherichia coli BL21 (DE3) cells. The gene coded for a protein that was 62.78 kDa. We purified and characterized the protein using Ni-NTA
affinity chromatography and Anti-His antibody Western blotting, respectively. Our results provide a basis for further studies
into the utility of the FlaA protein as a vaccine candidate against infection by Vibrio parahaemolyticus. In addition, the purified FlaA protein can be used for further functional and structural studies. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
