| Isolation, cloning and sequencing of AFLP markers related to disease-resistance traits in Fenneropenaeus chinensis |
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| 作者姓名： | 岳志芹 王伟继 孔杰 戴继勋 |
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| 作者单位： | [1]Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Yellow Sea Fisheries Research Institute, Qingdao 266071, China [2]College of Marine Life Sczence, Ocean University of China, Qingdao 266003, China [3]Food Laboratory of Qingdao Entry-Exit Inspection and Quarantine Bureau, Qingdao 266001, China |
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| 基金项目： | This research was supported by special funds from the National Key Basic Research Program （G1999012007） and the National High-Tech Research and Development Program of China （863 Program, 2001AA620105）. |
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| 摘 要： | Amplified fragment length polymorphisms （AFLP） technique was used to analyze the fingerprinting of four successive generations of Fenneropenaeus chinensis to reveal their disease-resistance traits. Some loci showed quite different genetic frequencies due to artificial selection, which implied that these fragments were putative markers related to the disease-resistance trait. We developed a simple and effective method to further characterize these AFLP fragments. Specific AFLP bands were cut directly from polyacrylamide gels, re-amplified, cloned and sequenced. Eight putative genetic markers were sequenced and their sizes ranged from 63 to 209 bp. The sequences were submitted to dbGSS （database of Genome Sequence Survey）; and the BLAST analysis showed low similarity to the function genes, indicating these markers were tightly linked to a disease-resistance trait but were not functional genes.
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| 关 键 词： | AFLP 抗病性 克隆技术 基因序列 分离工艺 渤海 海洋提取物 |
| 收稿时间： | 2003-12-03 |
| 修稿时间： | 2004-07-02 |
Isolation, cloning and sequencing of AFLP markers related to disease-resistance traits in Fenneropenaeus chinensis |
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| YUE Zhiqin, WANG Weiji, KNG Jie, DAI Jixun.Isolation, cloning and sequencing of AFLP markers related to disease-resistance traits in Fenneropenaeus chinensis[J].Chinese Journal of Oceanology and Limnology,2005,23(4):442-447. |
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| Authors: | YUE Zhiqin WANG Weiji KNG Jie DAI Jixun |
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| Institution: | YUE Zhiqin, WANG Weiji, K0NG Jie, DAI Jixun |
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| Abstract: | Amplified fragment length polymorphisms (AFLP) technique was used to analyze the fingerprint- ing of four successive generations of Fenneropenaeus chinensis to reveal their disease-resistance traits. Some loci showed quite different genetic frequencies due to artificial selection, which implied that these fragments were putative markers related to the disease-resistance trait. We developed a simple and effective method to fur- ther characterize these AFLP fragments. Specific AFLP bands were cut directly from polyacrylamide gels, re-amplified, cloned and sequenced. Eight putative genetic markers were sequenced and their sizes ranged from 63 to 209 bp. The sequences were submitted to dbGSS (database of Genome Sequence Survey); and the BLAST analysis showed low similarity to the function genes, indicating these markers were tightly linked to a dis- ease-resistance trait but were not functional genes. |
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| Keywords: | AFLP Fenneropenaeus chinensis disease-resistance trait markers clone sequence |
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