| The comparison between allozyme and RAPD makers for the population genetic structure analysis of scallop Chlamys farreri |
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| 作者姓名： | 刘保忠 尤锋 董波 相建海 |
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| 作者单位： | Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China，Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China，Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China，Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China |
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| 基金项目： | Supported by Chinese Basic Research Project (G1999012007) and Na-tional Natural Science Foundation of China (No. 39700017). |
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| 摘 要： | 1INTRODUCTION Since the first use in human genetic research in1966,allozyme has become the most popular genetic marker for population genetics studies for almost all groups of animals and plants(Lewontin and Hubby,1966;Murphy et al.,1996).In the past seve…
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| 关 键 词： | 异型酶 RAPD 遗传结构 分子标记 海洋生物 |
| 收稿时间： | 2004-07-26 |
| 修稿时间： | 2005-03-16 |
The comparison between allozyme and RAPD makers for the population genetic structure analysis of scallopChlamys farreri |
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| Liu Baozhong,You Feng,Dong Bo,Xiang Jianhai.The comparison between allozyme and RAPD makers for the population genetic structure analysis of scallop Chlamys farreri[J].Chinese Journal of Oceanology and Limnology,2006,24(3):295-299. |
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| Authors: | Liu Baozhong You Feng Dong Bo Xiang Jianhai |
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| Institution: | (1) Institute of Oceanology, Chinese Academy of Sciences, 266071 Qingdao, China |
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| Abstract: | To compare genetic markers for population genetics analysis, allozyme electrophoresis and random amplified polymorphic DNA (RAPD) were used to detect the genetic structure of scallop Chla- mys farreri population. Thirteen enzymes (MDH, ME, IDH, GPI, PGM, PEP-LG, PEP-PP, ACP, AK, PK, AAT, SOD, EST) in three buffer systems (TC, pH6.9; TMME, pH 7.4; and EBT, pH8.9) were selected and 22 loci were used for the analysis, among them 7 loci (Gpi, Pgm, Pep-LG-1, Pep-PP, Aat-2, Est-2, Est-3) were polymorphic which attributed 31.82% to the total. The average of heterozygosity was 0.113 and most of the studied loci showed heterozygote deficiencies. The same specimens were investigated using 10 arbitrarily selected primers (10-base). Twenty two of 54 RAPD fragments were polymorphic with av- erage heterozygosity of 0.194. The result indicated that the two types of markers reflected a consistent trend in the parameter values of genetic diversity of the population, but RAPD revealed more information of genetic variation than allozyme electrophoresis. |
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| Keywords: | Allozyme RAPD Chlamys farreri population genetic structure |
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