不同盐碱环境中宁夏枸杞叶生理特征和RAPD分析

调查了生长在不同盐碱地宁夏枸杞(LyciumbarbarumL.)的生理特征。研究发现,次生盐碱地对枸杞的影响要大于原生盐碱地,光合色素含量、脯氨酸含量、蛋白质和核酸均表现为生长在次生盐碱地的枸杞体内含量高,但在幼株和成年植株体内的变化各不相同。可溶性蛋白凝胶电泳显示,对不同年龄的植株在同样的生境中谱带条数和着色深浅基本一样,但不同生境中有两条谱带的着色深浅有明显的不同。分析表明,光合色素含量、脯氨酸含量和蛋白质代谢的这种变化可能是植物的一种适应性反应,它有利于植物抵抗盐胁迫的伤害。RAPD分析表明宁夏枸杞在生长发育过程中,为了适应环境,在代谢发生了变化后,其遗传物质DNA也发生了一定的变异。

Physiological Features and RAPD Analysis of Lycium Barbarum Leaves in Different Saline Habitats

The physiological features of Lycium barbarum, growing in different saline habits by its origin, primary or secondary, were compared and analyzed in this paper. The results show that the impact of secondary saline habitat on Lycium barbarum is stronger than that of primary saline habitat; the amount of photosynthetic pigment, proline, protein and nucleic acid in the leaves of the plants lived in secondary saline habitat is larger than that of living in primary saline habitats but varies in juvenile and adult plants. The experiment of electrophoresis of soluble protein gel indicated that the number and coloring of the spectrum belts of uneven-aged Lycium barbarum planted in same sites are similar, while two belts with distinctly different color appear in the spectrum of the plants situated in different environment. The changes of the content levels of photosynthetic pigment, proline and the protein metabolism may imply an adaptive response of the plants, which favors the resisting of plants to salt stress. RAPD analysis showed that DNA variation, which followed metabolism modification in the plant, occurred to be adapted to the salt environment with the growth and development of Lycium barbarum.