生物甲烷气实验的外加菌源(沼液)中厌氧菌群测定

考虑到煤本源微生物的分离、纯化及培养相对困难,且耗时较长,采用沼液作为外加菌源,并在进行生物甲烷气实验之前,对所采集沼液中的主要厌氧菌群进行微生物特征及数量的测定。结果表明沼液中的厌氧发酵菌(Anaerobicfermentative bacteria)、厌氧纤维素分解菌(Anaerobic cellulose-decomposing bacteria)、硫酸盐还原菌(Sulfate-reducingbacteria)和产甲烷菌(Methanogens)4类厌氧菌群,均具有杆状、球状及链状等多种形态;染色后发现其中革兰氏阴性菌占主要优势;经最大或然数(MPN)计数后表明,沼液中所含厌氧菌的数量足以进行后续的生物甲烷气的产出实验。

Determination of Anaerobic Bacterial Consortia in Added Microbial Sources (Biogas Slurry) for Biogenic Methane Experiment

Given that indigenous microbial consortia of raw coal samples are in great difficulty being separated, purificated and cultured, which are invariably at a big cost of time, biogas slurry was considered to be added as the microbial sources, of which the morphological characteristics and the amount of dominant anaerobic bacteria were determined prior to the conduction of biogenic-methane-production experiment. The results show that four types of anaerobic microbial consortia including fermentative bacteria, cellulolytic bacteria, sulfate-reducing bacteria and methanogens were separately indentified and observed with such recognizable morphological features as rod-shaped, spherical and streptobacteria; the gram-negative bacteria were found to be at a notable advantage after gram stain; further the content of microbial consortia determined by most probable number (MPN) counting method was deemed to be enough for following biogenic-methane-production experiment.