| 高效纤维素降解菌系的构建 |
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| 引用本文： | 李平,王焰新,刘琨,王艳红,童蕾.高效纤维素降解菌系的构建[J].地球科学,2009,34(3):533-538. |
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| 作者姓名： | 李平 王焰新 刘琨 王艳红 童蕾 |
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| 作者单位： | 中国地质大学环境学院, 生物地质与环境地质教育部重点实验室, 湖北武汉 430074 |
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| 基金项目： | 国家杰出青年科学基金，世界自然科学基金会资助课题 |
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| 摘 要： | 筛选出能产生不同纤维素酶的10株纤维素降解菌, 系统地分析了各菌株的EG、CBH和BG酶等3类纤维素酶活.经各菌株优化组合、混合培养, 构建了一组由5株细菌(LCB03、LCB12、LCB52、LCD12和LCD51)组成、能协同作用的复合微生物菌系.经生理生化和分子水平鉴定, 这5株细菌分别为Pseudomonas citronellolis(香茅醇假单胞菌)、Stenotrophomonas malto-philia(嗜麦芽寡食单胞菌)、Pseudomonas aeruginosa(铜绿假单胞菌)、Pseudomonas aeruginosa(铜绿假单胞菌)和Flavobacterium mizutaii(水氏黄杆菌).复合菌系的各菌株可产生不同类型的纤维素酶, 且各类酶可以协同作用有效分解天然纤维素, 在纤维素类污染的治理与资源化利用中具有很好的应用前景.
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| 关 键 词： | 纤维素降解 复合菌系 构建 鉴定 |
| 收稿时间： | 2009-01-12 |
Construction of A Microbial System for Efficient Degradation of Cellulose |
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| LI Ping,WANG Yan-xin,LIU Kun,WANG Yan-hong,TONG Lei School of Environmental Studies,MOE Key Laboratory of Biogeology , Environmental Geology,China University of Geosciences,Wuhan ,China.Construction of A Microbial System for Efficient Degradation of Cellulose[J].Earth Science-Journal of China University of Geosciences,2009,34(3):533-538. |
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| Authors: | LI Ping WANG Yan-xin LIU Kun WANG Yan-hong TONG Lei School of Environmental Studies MOE Key Laboratory of Biogeology Environmental Geology China University of Geosciences Wuhan China |
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| Institution: | LI Ping,WANG Yan-xin,LIU Kun,WANG Yan-hong,TONG Lei School of Environmental Studies,MOE Key Laboratory of Biogeology , Environmental Geology,China University of Geosciences,Wuhan 430074,China |
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| Abstract: | Endoglucanase,exoglucanase and cellobiase activities of ten cellulose degradation isolates from piggery sludge and manure were analyzed.A complex microbial system was constructed by five bacteria named as LCB03,LCB12,LCB52,LCD12 and LCD51 respectively with different cellobiase activities from these isolates.Tested from the perspective of physiology and microchemistry as well as molecular level,these five bacteria were identified as Pseudomonas citronellolis,Stenotrophomonas maltophilia,Pseudomonas aeruginos... |
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| Keywords: | cellulose biodegradation complex microbial system construction identification |
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