| 原油生物降解模拟实验及其定量化评价 |
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| 引用本文： | 向廷生,黑花丽.原油生物降解模拟实验及其定量化评价[J].现代地质,2010,24(2):259-267. |
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| 作者姓名： | 向廷生 黑花丽 |
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| 作者单位： | 1.长江大学 油气资源与勘探技术教育部重点实验室,湖北 荆州434023；
2.长江大学 地球化学系,湖北 荆州434023 |
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| 摘 要： | 利用筛选到的优势烃降解复合菌(I菌)对大庆油田3口不同油井的油(西5-P10、三元后和G1131-262)进行不同时间的降解实验和全油GC-MS定量分析，探讨饱和烃、芳烃化合物分布情况变化。实验研究表明I菌为高效烃降解菌；相同微生物对此3种不同原油的降解能力存在明显的差异，所以烃污染现场生物修复试验需要根据不同原油性质选择不同的高效降解菌；对于饱和烃和芳烃生物降解的顺序既有对过去结论的验证又提出新的看法。藿烷的降解在重排甾烷之后，萘比菲先开始降解,三甲基萘比三甲基菲更早开始遭受生物降解，三甲基和四甲基萘在深度生物降解后会达到一个平衡，之后的降解速度减慢。当生物降解到一定阶段，抗生物降解能力强的多环芳烃富集会加重对环境的毒害，因此，多环芳烃降解菌或萘、菲降解菌等特效菌是未来烃污染环境修复工作的重点。
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| 关 键 词： | 生物降解 复合菌 GC-MS 饱和烃 芳烃 |
A Biodegradation Experiment of Crude Oils in Laboratory and Quantitative Evaluation |
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| XIANG Ting-sheng,HEI Hua-li.A Biodegradation Experiment of Crude Oils in Laboratory and Quantitative Evaluation[J].Geoscience——Journal of Graduate School,China University of Geosciences,2010,24(2):259-267. |
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| Authors: | XIANG Ting-sheng HEI Hua-li |
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| Institution: | 1.MEO Key Laboratory of Petroleum Resources and Explortion Technology, Yangtze University, Jingzhou, Hubei 434023, China;
2. Department of Geochemistry，Yangtze University,Jingzhou, Hubei434023,China |
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| Abstract: | In this study,effective hydrocarbon degrading mixed bacteria(I strain) has been obtained by screening,and three kinds of oil(Xi 5-P10,Sanyuanhou,G1131-262) from three different wells respectively in Daqing oilfield have been degraded by experiments with different time intervals.Then GC-MS analysis has been carried out on the oils.The change of the distribution of the saturated hydrocarbons and aromatic hydrocarbons of the oil is probed into by quantitative analysis.The results show that strain I is a kind o... |
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| Keywords: | GC-MS |
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