菲降解菌的分离鉴定及其降解性研究

采用微生物富集培养分离法对南洋油田不同油样中的菲降解菌进行了分离鉴定。分离得到2株以菲作为唯一碳源和能源的细菌菌株S17和S28,经革兰氏染色及显微镜形态观察,发现两者都是具有极生单鞭毛的革兰氏阴性、无芽孢杆菌。根据生理生化特性分析,以及16S rDNA序列同源性分析,两者属于假单胞菌属的不同种。菌株S17与食树脂假单孢菌(Pseudomonas resinovorans)序列同源性为97%;菌株S28则为高温假单胞菌(Pseudomonas thermaerum),其序列同源性达到100%。在以菲为唯一底物的条件下,菌株S28的生长速度是S17的2倍多,第4天即达到最大生长量,但是对于菲的降解而言,两者都在第10天达到最大,降解率分别为88.86%和82.02%,但是二者在起始的两天内对菲的降解效率最高,分别达到70.21%和72.74%,因此可用于菲污染的快速治理领域。

Isolation, identification and characteristics of phenanthrene-degrading bacteria

Two bacterial strains,S17 and S28,were isolated and identified using inorganic salt medium from different oil samples of Nanyang oil field,which could be able to use phenanthrene as sole carbon and energy sources.They were G-strains with only one flagellum on one side of the cell and without spore detected using microscope and stainning.The strains were identified as Pseudomonas species on the basis of biochemical tests and 16S rDNA sequences assay.The homology was 97% between the strain S17 and P.resinovorans,and the strain S28 was P.thermaerum.The growth speed of S28 was twice as large as that of S17 and reached maximum concentration within 4 days in the inorganic salt medium with phenanthrene as sole carbon source.The maximum degradation rates of phenenthrene of S17 and S28 were 88.86% and 82.02% respectively within 10 days.