海洋真菌ZJ27 次级代谢产物的抑菌活性的研究

对从南海海藻内生真菌ZJ27次级代谢产物中分离得到的5种化合物进行抑菌活性的研究,采用96孔板法测定化合物对蜡状芽孢杆菌、枯草芽孢杆菌、大肠杆菌、白色葡萄球菌、藤黄八叠球菌、金黄色葡萄球菌等6种细菌的抑制活性,并用生长速率法测定化合物对黑曲霉、尖孢镰刀菌、立枯丝核菌和白色念珠菌等4种真菌的抑制活性。化合物对上述6种细菌均有不同的抑制作用,其中化合物1对枯草芽孢杆菌、白色葡萄球菌和藤黄八叠球菌有很好的抑制作用,MIC值分别为0.1、0.2、0.2μmol/mL。化合物1和2对黑曲霉、尖孢镰刀菌、立枯丝核菌和白色念珠菌也有很好的抑制作用,化合物1对这4种真菌的IC50分别为15.06、3.09、3.68、8.19μmol/mL,化合物2相应的IC50为22.28、21.32、8.18、21.75μmol/mL。化合物1和2作为天然抗菌物质,具有广谱抑菌效果,具有较高的应用潜力和综合开发前景。

Antibacterial activity of secondary metabolites of marine fungi ZJ27

The antibacterial activities of five compounds isolated from secondary metabolites of seaweed endophytic fungi ZJ27 were studied in Bacillus cereu,Bacillus subtilis,Escherichia coli,Staphylococcus albus,Sarcina luteus,and Staphylococcus aureus and the antifungal activities were tested in Aspergillus niger,Fusarium oxysporum,Rhizoctonia solani,and Candida albicans by the growth rate method.Different compounds had diffident antibacterial activities in the six bacteria.Compound 1 showed remarkable inhibition of B.subtilis,S.albus and S.luteus with an MIC of 0.1,0.2,and 0.2 μmol/mL,respectively.Compounds 1 and 2 had notable inhibition of A.niger,F.oxysporum,R.solani and C.albicans,with the IC50 values of compound 1 being 5.06,3.09,3.68,and 8.19 μmol/mL and those of compound 2 were 22.28,21.32,8.18,and 21.75 μmol/mL.As natural antibacterial substances,Compounds 1 and 2 have a great range of potential utilities and a prospect of development and are worth further study.